• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.34 no.3
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• pp.250-265
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• 2008

Distraction osteogenesis is a well-established clinical treatment for limb length discrepancy and skeletal deformities. Appropriate mechanical tension-stress is believed not to break the callus but rather to stimulate osteogenesis. In contrast to fracture healing, the mode of bone formation in distraction osteogenesis is primarily intramembranous ossification. Although the biomechanical, histological, and ultrastructural changes associated with distraction osteogenesis have been widely described, the basic biology of the process is still not well known. Moreover, the molecular mechanisms in distraction osteogenesis remain largely unclear. Recent studies have implicated the growth factor cascade is likely to play an important role in distraction. And current reserch suggested that mechanical tension-stress modulates cell shape and phenotype, and stimulates the expression of the mRNA for bone matrix proteins. The purpose of this study is to examine the pattern of expression of growth factors($TGF-{\beta}1$, IGF-I, bFGF) and extracellular matrix proteins(osteoclacin, osteonectin) related to osteogenesis by osteodistraction of the mandible in rabbits. 24 rabbits is used for this experiment. Experimental group are gradual distraction(0.7mm, twice/day), acute distraction(1.4mm, twice/day) and control group is only osteotomized. After 5 days latency, osteotomic site is distracted for each 7 days and 3.5 days. Consolidation period is 28 days. The animal is sacrificed at the 3th, 7th, 14th, 28th. The distracted bone is examined by immunohistochemical analysis and RT-PCR analysis. The results obtained from this study were as follow : No significant difference was found on clinical examination according to distraction rate, but gradual distraction was shown to improve regenerate bone formation on radiographic and histologic examination. Growth factors and extracelluar matrix proteins expression increased in distraction group than control group. From these results, it could be stated that graudal distraction is shown to improve and accelerate bone formation and mechanical stress like distraction has considerable effects on osteogenesis related factors. And rabbit is the most appropriate animal model for further reseach on the molecular mechanisms that mediate osteodistraction. It is believed that understanding the biomolecular mechanisms that mediate distraction osteogenesis may guide the development of targeted strategies designed to improve distraction osteogenesis and accelerate bone healing.

• Journal of Animal Science and Technology
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• v.45 no.6
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• pp.891-900
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• 2003

Cellular FOS(c-fos) protein is a transcription factor that forms heterodimers mostly with c-jun family and stimulates the transcription of genes containing AP-1 regulatory elements. This c-fos expression can control growth and differentiation of various precursor cells including myoblasts. The controls by c-fos gene have been identified for affecting skeletal muscle fiber traits which are the key determinants of meat quality in pigs. As a first step for identifying the relationship between c-fos gene and meat quality traits in cattle, we fully sequenced 1,443 bp of Hanwoo c-fos mRNA and analyzed expression patterns from various organs and muscle tissues. The sequence identities of Hanwoo c-fos with that of human, pig and mouse showed 89.8%, 93.3% and 87%, respectively. Analyses of the northern blot showed high c-fos expressions were obtained in spleen and rib muscle from 7 organs and 9 different parts of muscles investigated. These results presented here can be used as a valuable marker for meat quality related traits in cattle with further verification.

• The korean journal of orthodontics
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• v.40 no.6
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• pp.411-420
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• 2010

Objective: The purpose of this study was to assess the changes in height and inclination of the articular eminence during the growth period. Methods: One hundred and sixty subjects (71 males and 89 females) with a normal skeletal pattern and TMJ function, ranging in age from 5.9 to 19.7 years were divided according to their chronological age into six groups. Lateral individualized corrected TMJ tomograms were taken of all subjects, and the height and inclination of the articular eminence were measured. UNIANOVA was used to compare the differences between the age groups. Mann-Whitney test was used to compare the differences between male and female subjects. Results: The height and the inclination of the articular eminence were increased and became steeper with age, and the height and the inclination were larger in male than in female subjects. Conclusions: Dynamic changes in the height and the inclination of the articular eminence were observed during the growth period, therefore a full understanding of the growth of the articular eminence is important for orthodontic and orthopedic treatment in this period.

• The korean journal of orthodontics
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• v.32 no.6 s.95
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• pp.413-424
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• 2002

Preadolescent children with deficient maxillae are suitable candidates for the maxillary protraction appliance(MPA). The theoretical effect of the MPA is protraction or anterior displacement of the maxilla. However, it is known that complex effects such as anterior displacement of the maxillary teeth, downward and backward rotation of the mandible, linguoversion of the mandibular anterior incisors, are known to play a role in improving the Cl III malocclusion. There have been much studies with regard to maxillary protraction, but the different effects of MPAs depending on the vertical facial pattern are not known precisely. This study was based on 67 patients (31 males, 36 females) aged from 6 years 6 months to 13 years 3months, who visited the Dept. of Orthodontics at Yonsei Univ., Dental Hospital and diagnosed as skeletal Class III with maxillary deficiency. They were divided into 3 groups (low, average, high angle groups) depending on genial angle and the SNMP (Go-Gn) angle, respectively. Pretreatment and post-treatment lateral cephalograms were used to compare the effects of MPA and the following conclusions were obtained: 1) A significantly large amount of backward movement of the B point was observed in patients with a low SNMP angle. Those with a high SNMP angle had significant forward movement at A point. 2) The patients with low genial angle had the least forward movement at the A point, and those with a high angle had more forward movement. 3) In comparing the arcTan of the A point, the high angle group showed more horizontal movement while the low angle group showed more vertical movement. 4) There was no significance between the treatment duration of the SNMP and the Genial angle groups.

• Journal of the korean academy of Pediatric Dentistry
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• v.44 no.2
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• pp.129-137
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• 2017

The aim of this study was to enhancing the panoramic radiograph's clinical use for assessing mandibular measurements and formulating a function of those measurements from panoramic radiographs and lateral cephalograms in children. The panoramic radiographs and lateral cephalograms of 99 former orthodontic patients with skeletal class III malocclusion were selected. In each radiograph, gonial angles, ramus heights, and distance between lower incisors and symphysis were measured. The values of the studied parameters were compared by paired t-test, Pearson's correlation test and regression analysis. The mean value of the gonial angle in panoramic radiographs was $125.49^{\circ}$, and the value in lateral cephalograms was $127.50^{\circ}$. The Pearson's correlation coefficient (${\rho}$) between mean values of gonial angle in each radiograph was 0.945 (p < 0.001). The relationship between the gonial angle measurements obtained from each radiographs was represented as 'Gonial angle (Lateral cephalograms) = 0.920 ${\times}$ Average gonial angle (Panoramic radiographs) + 12.072' in the linear function. The coefficients of ramus heights, and distance between lower incisors and symphysis portrayed weaker correlations than gonial angles. A panoramic radiograph could be used to determine the gonial angle as accurately as a lateral cephalogram, and each gonial angle showed a strong positive relation. A panoramic radiograph is a useful tool for examining vertical growth pattern of patients, as well as a lateral cephalogram.

• The korean journal of orthodontics
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• v.25 no.1 s.48
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• pp.1-12
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• 1995

The purpose of this study was to predict the respose to the chincap therapy from the initial cephalometric measurements and to obtain the indication of chincap therapy. 40 patients selected for this study were classified into two groups by the occlusal stability after completion of permanent dentition and the improvement of facial profile, after chincap therapy. One was good response group which consisted of 25 children and the other was poor response group with 15 patients. Various measurements of the craniofacial structure in the initial lateral cephalogram were calculated and analyzed by t-test and discriminant analysis. The results were as follows: 1. Good response group had more horizontal growth pattern in initial stage of treatment, and the contributing measurements were $Bj\ddot{o}rk$ sum anterior-posterior facial height ratio, genial angle, lower genial angle and occlusal plane to AB plane angle. 2. The critical points and predictive values of the influential skeletal measurements were calculated. 3. The discriminant function was obtained from three major influential measurements; $Bj\ddot{o}rk$ sum, genial angle and occlusal plane to hn plane angle, and this function could discreminate correctly in $85\%$ of this samples.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.3
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• pp.313-318
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• 2009

TIAF1 is a TGF-${\beta}$1-induced anti-apoptotic factor that plays a critical role in blocking TNF (tumor necrosis factor) cytotoxicity in mouse fibroblasts and participates in TGF-${\beta}$-mediated growth regulation. In this study, we obtained the full-length cDNA sequence of the porcine TIAF1 gene. Real-time PCR further revealed that the TIAF1 gene was expressed at the highest level in liver and kidney with prominent expressions detected in uterus, and lower levels detected in heart, spleen, lung, stomach, small intestine, skeletal muscle and fat of Large White pigs. Sequence analysis indicated that a 6 base-pair deletion mutation existed in the exon of the TIAF1 gene between Meishan and Large White pigs. This mutation induced deletion of Gln and Val amino acids. PCR-RFLP was used to detect the polymorphism in 394 pigs of a "Large White${\times}$Meishan" $F_{2}$ resource population and four purebred pig populations. The frequencies of the A allele (with a 6 bp deletion) were dominant in Chinese Meishan and Bamei pigs, and the frequencies of the B allele (no 6 bp deletion) were dominant in Large White and Landrace pigs. Association analyses revealed that the deletion mutation had highly significant associations (p<0.01) with meat marbling score of the thorax-waist longissimus dorsi (LD) muscle (MM1) and intramuscular fat percentage (IMF), and significant associations (p<0.05) with carcass length (CL). The results presented here supply evidence that the 6 bp deletion mutation in the TIAF1 gene affects porcine meat quality and provides useful information for further porcine breeding.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.3
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• pp.398-407
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• 2020

Objective: The Yip1 domain family (YIPF) proteins were proposed to function in endoplasmic reticulum (ER) to Golgi transport and maintenance of the morphology of the Golgi, which were homologues of yeast Yip1p and Yif1p. YIPF3, the member 3 of YIPF family was a homolog of Yif1p. The aim of present study was to investigate the expression and regulation mechanism of porcine YIPF3. Methods: Quantitative realtime polymerase chain reaction (qPCR) was used to analyze porcine YIPF3 mRNA expression pattern in different tissues and pig kidney epithelial (PK15) cells stimulated by polyinosine-polycytidylic acid (poly [I:C]). Site-directed mutations combined with dual luciferase reporter assays and electrophoretic mobility shift assay (EMSA) were employed to reveal transcription regulation mechanism of porcine YIPF3. Results: Results showed that the mRNA of porcine YIPF3 (pYIPF3) was widely expressed with the highest levels in lymph and lung followed by spleen and liver, while weak in heart and skeletal muscle. Subcellular localization results indicated that it expressed in Golgi apparatus and plasma membranes. Upon stimulation with poly (I:C), the level of this gene was dramatically up-regulated in a time- and concentration-dependent manner. pYIPF3 core promoter region harbored three cis-acting elements which were bound by ETS proto-oncogene 2 (ETS2), zinc finger and BTB domain containing 4 (ZBTB4), and zinc finger and BTB domain containing 14 (ZBTB14), respectively. In which, ETS2 and ZBTB4 both promoted pYIPF3 transcription activity while ZBTB14 inhibited it, and these three transcription factors all played important regulation roles in tumorigenesis and apoptosis. Conclusion: The pYIPF3 mRNA expression was regulated by ETS2, ZBTB4, and ZBTB14, and its higher expression in immune organs might contribute to enhancing ER to Golgi transport of proteins, thus adapting to the immune response.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.23 no.1
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• pp.48-58
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• 2001

After performing mandibular setback surgery, we found some changes in patterns and organs of speech. This investigation was undertaken to investigate the aspect and degree of speech patterns according to the amount of surgical setback in mandibular prognathic patients. Thirteen patients with skeletal Class III malocclusion were studied preoperative and postoperative over 6 months. They had undergone the mandible setback operation via bilateral sagittal split ramus osteotomy(BSSRO). We split the patients into two groups. Group 1 included patients whose degree of mandibular setback was 6mm or less, and Group 2 above 6mm. Control group was two adults wish normal speech patterns. A phonetician performed narrow phonetic transcriptions of tape-recorded words and sentences produced by each of the patients and the acoustic characteristics of the plosives, fricatives, and flaps were analyzed with a phonetic computer program (Computerized Speech Lab(CSL) Model 4300B(USA)). The results are as follows: 1. Generally, Patients showed longer closure duration of plosives, shorter VOT(voice onset time) and higher ratio of closure duration against VOT. 2. Patients showed more frequent diffuse distribution than the control group in frication noise energy of fricatives. 3. In fricatives, frequency of compact from were higher in group 1 than in group 2. 4. Generally, a short duration of closure for /ㄹ/ was not realized in the patient's flaps. Instead, it was realized as fricatives, sonorant with a vowel-like formant structure, or trill type consonant. 5. Abnormality of the patient's articulation was reduced, but adaptation of their articulation after surgery was not perfect and the degree of adaptation was different according to the degree of surgical setback.

• Journal of the korean academy of Pediatric Dentistry
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• v.32 no.1
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• pp.109-118
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• 2005

In the field of pediatric dentistry, comparison and analysis of cephalogram values of children are important fir evaluation of growth and development, and are essential to evaluate the craniofacial form and growth pattern for early diagnosis of malocclusion. For this, cephalographic norm values are important, but not many studies on the primary dentition exist. To compare the past norm values of normal occlusion in the primary dentition with current norms, preschool children, 4 to 5 years of age, with normal occlusion in the primary dentition who visited our hospital were examined. Among these children, 46 children with normal facial form and developmental status were chosen for evaluation of cephalogram values. The following results were as follows: 1. For skeletal values, the angular values showed no significant differences between males and females, and the linear values were generally greater in males than females. 2. SNA was $81.3^{\circ}$, SNB was $76.6^{\circ}$ and ANB difference was $4.7^{\circ}$. 3. The ratio for Mandibular body length to Anterior cranial base length was 0.9 : 1 for both male and female and the ratio for posterior facial height to anterior facial height was 61.4 % for male, 62.0 % for female. 4. For dental values, IMPA was $84.2^{\circ}$ and UA to SN was $90.8^{\circ}$. 5. The upper lip to Ricketts esthetic line was positioned 2.6 mm anteriorly, and the lower lip to Ricketts esthetic line was positioned 2.5 mm anteriorly.