Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Isolation, Expression Pattern, Polymorphism and Association Analysis of Porcine TIAF1 Gene

  • Wang, Y. (Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture and Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University) ;
  • Xiong, Y.Z. (Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture and Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University) ;
  • Ren, Z.Q. (Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture and Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University) ;
  • Zuo, B. (Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture and Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University) ;
  • Lei, M.G. (Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture and Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University) ;
  • Deng, C.Y. (Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture and Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University)
  • Received : 2008.07.20
  • Accepted : 2008.10.27
  • Published : 2009.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

TIAF1 is a TGF-${\beta}$1-induced anti-apoptotic factor that plays a critical role in blocking TNF (tumor necrosis factor) cytotoxicity in mouse fibroblasts and participates in TGF-${\beta}$-mediated growth regulation. In this study, we obtained the full-length cDNA sequence of the porcine TIAF1 gene. Real-time PCR further revealed that the TIAF1 gene was expressed at the highest level in liver and kidney with prominent expressions detected in uterus, and lower levels detected in heart, spleen, lung, stomach, small intestine, skeletal muscle and fat of Large White pigs. Sequence analysis indicated that a 6 base-pair deletion mutation existed in the exon of the TIAF1 gene between Meishan and Large White pigs. This mutation induced deletion of Gln and Val amino acids. PCR-RFLP was used to detect the polymorphism in 394 pigs of a "Large White${\times}$Meishan" $F_{2}$ resource population and four purebred pig populations. The frequencies of the A allele (with a 6 bp deletion) were dominant in Chinese Meishan and Bamei pigs, and the frequencies of the B allele (no 6 bp deletion) were dominant in Large White and Landrace pigs. Association analyses revealed that the deletion mutation had highly significant associations (p<0.01) with meat marbling score of the thorax-waist longissimus dorsi (LD) muscle (MM1) and intramuscular fat percentage (IMF), and significant associations (p<0.05) with carcass length (CL). The results presented here supply evidence that the 6 bp deletion mutation in the TIAF1 gene affects porcine meat quality and provides useful information for further porcine breeding.

Keywords

References

  1. Bachem, M. G., K. M. Sell, R. Melchior, J. Kropf, T. Eller and A. M. Gressner. 1993. Tumor necrosis factor alpha (TNF alpha) and transforming growth factor beta 1 (TGF beta 1) stimulate fibronectin synthesis and the transdifferentiation of fat-storing cells in the rat liver into myofibroblasts. Virchows. Arch. (B). 63:123-130 https://doi.org/10.1007/BF02899251
  2. Bonewald, L. F. 1999. Regulation and regulatory activities of transforming growth factor beta. Crit. Rev. Eukaryot. Gene Expr. 9:33-44
  3. Chang, N. S., J. Mattison, H. Cao, N. Pratt, Y. Zhao and C. Lee. 1998. Cloning and characterization of a novel transforming growth factor-b1-induced TIAF1 protein that inhibits tumor necrosis factor cytotoxicity. Biochem. Biophys. Res. Commun. 253:743-749 https://doi.org/10.1006/bbrc.1998.9846
  4. Chang, N. S. 2003. TIAF1 participates in the TGF-beta signaling by interacting with Smad proteins. Proceedings of the American Association for Cancer Research Annual Meeting 44:1038 (Abstr.)
  5. Chang, N. S. 2004. Inhibition of nuclear translocation of serine 15-phosphorylated p53 by silencing TIAF1. FASEB J. 18(Suppl.S):556(Abstr.)
  6. Chen, J. F., L. H. Dai, J. Peng, J. L. Li, R. Zheng, B. Zuo, F. E. Li, M. Liu, K. Yue, M. G. Lei, Y. Z. Xiong, C. Y. Deng and S. W. Jiang. 2008. New evidence of alleles (V199I and G52S) at the PRKAG3 (RN) locus affecting pork meat quality. Asian-Aust. J. Anim. Sci. 21:471-477
  7. Dai, L. H., Y. Z. Xiong, C. Y. Deng, S. W. Jiang, B. Zuo, R. Zheng, F. E. Li and M. G. Lei. 2006. Association of the A-G polymorphism in porcine adiponectin gene with fat deposition and carcass traits. Asian-Aust. J. Anim. Sci. 19:779-783
  8. Gong, W. H., Z. L. Tang, J. L. Han, S. L. Yang, H. Wang, Y. Li and K. Li. 2008. Mapping, tissue distribution and polymorphism of porcine retinol binding protein genes (RBP5 and RBP7). Asian-Aust. J. Anim. Sci. 21:1544-1550
  9. Hellerbrand, C., B. Stefanovic, F. Giordano, E. R. Burchardt and D. A. Brenner. 1999. The role of TGF$\beta$1 in initiating hepatic stellate cell activation in vivo. J. Hepatol. 30:77-87 https://doi.org/10.1016/S0168-8278(99)80010-5
  10. Khera, S. and N. S. Chang. 2003. TIAF1 participates in the transforming growth factor $\beta$1-mediated growth regulation. Ann. N. Y. Acad. Sci. 955:11-21 https://doi.org/10.1111/j.1749-6632.2002.tb02761.x
  11. Lawrence, D. A. 1996. Transforming growth factor-beta: a general review. Eur. Cytokine. Netw. 7:363-374 https://doi.org/10.1136/thorax.56.12.907
  12. Liang, P. and A. B. Pardee. 1992. Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Sci. 257:967-970 https://doi.org/10.1126/science.1354393
  13. Liu, B.H. 1998. Statistical genomics: linkage, mapping and QTL analysis. CRC Press, LLC
  14. Liu, G. Y., Y. Z. Xiong, C. Y. Deng, B. Zuo and J. H. Zhang. 2004. Comparison of gene expression patterns in Longissimus dorsi of pigs between the high-parent heterosis cross combination Landrace×Large White and the mid-parent heterosis cross combination Large White×Meishan. Asian-Aust. J. Anim. Sci. 17:1192-1196
  15. Ren, Z. Q., Y. Z. Xiong, C. Y. Deng and M. G. Lei. 2006. Cloning and identification of porcine SMPX differentially expressed in F1 crossbreds and their parents. Acta Biochim. Biophys. Sin. 38:753-758 https://doi.org/10.1111/j.1745-7270.2006.00229.x
  16. SAS. 1999. Statistical Analysis System institute, Incorporation. Cary, NC, USA
  17. Serra, J. J., M. Ellis and C. S. Haley. 1992. Genetic components of carcass and meat quality traits in Meishan and Large White pigs and their reciprocal crosses. Anim. Prod. 54:117-127 https://doi.org/10.1017/S0003356100020638
  18. Schultz, L., S. Khera, D. Sleve, J. Heath and N. S. Chang. 2004. TIAF1 and p53 functionally interact in mediating apoptosis and silencing of TIAF1 abolishes nuclear translocation of serine 15-phosphorylated p53. DNA Cell Biol. 23:67-74 https://doi.org/10.1089/104454904322745943
  19. Shull, M. M., I. Ormsby, A. B. Kier, S. Pawlowski, R. J. Diebold, M. Yin, R. Allen, C. Sidman, G. Proetzel and D. Calvin. 1992. Targeted disruption of the mouse transforming growth factorbeta1 gene results in multifocal inflammatory disease. Nature 359:693-699 https://doi.org/10.1038/359693a0
  20. ten Dijke, P., K. Miyazono and C. H. Heldin. 2000. Signaling inputs converge on nuclear effectors in TGF-beta signaling. Trends Biochem. Sci. 25:64-70 https://doi.org/10.1016/S0968-0004(99)01519-4
  21. van der Leij, J., A. van den Berg, E. W. Albrecht, T. Blokzijl, R. Roozendaal, A. S. Gouw, K. P. de Jong, C. A. Stegeman, H. van Goor, N. S. Chang and S. Poppema. 2003. High expression of TIAF-1 in chronic kidney and liver allograft rejection and in activated T-helper cells. Transplantation 75:2076-2082 https://doi.org/10.1097/01.TP.0000069829.71088.88
  22. Wang, T., L. Chang, Y. Z. Xiong, C. Y. Deng, B. Zuo, H. T. Xie and D. Q. Xu. 2007. Isolation and cloning of porcine SLC27A2 gene and detection of its polymorphism associated with growth and carcass traits. Asian-Aust. J. Anim. Sci. 20:1169-1173
  23. Xiong, Y. Z. and C. Y. Deng. 1999. Technical testing rule of lean pig. Chinese Agricultural Press, Beijing