• Korean Journal of Plant Taxonomy
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• v.44 no.4
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• pp.250-256
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• 2014

This study was carried out to understand the genetic relationship of three Aloe spp. cultivated in Korea, A. saponaria, A. vera and A. arborescens and a new variant in Korea based on three plastid (matK, trnL-F, rbcL) and one nuclear (ITS regions) DNA barcode markers. A total of 2,420 bp sequence was amplified. Two indels were detected in the trnL region, and also several species specific nucleotide loci were detected in all 29 parsimonious informative sites, and 148 variable sites were detected among four taxa studied while 170 variable and 75 parsimonious sites were detected when other Aloe spp. in worldwide were used. An UPGMA phenogram with 10,000 bootstrap replication showed that the new variant was closest to A. vera. The variant was not morphologically and genetically concurrent with any reported species so far. The clustering of Aloe species were broadly in agreement with previously reported results.

• Journal of Korea Multimedia Society
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• v.15 no.9
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• pp.1165-1173
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• 2012

One of the most popular technologies in Web2.0 is tagging, and it widely applies to Web content as well as multimedia data such as image and video. Web users have expected that tags by themselves would be reused in information search and maximize the search efficiency, but wrong tag by irresponsible Web users really has brought forth a incorrect search results. In past papers, we have gathered various information resources and tags scattered in Web, mapped one tag onto other tags, and clustered these tags according to the corelation between them. A 3-tag based search algorithm which use the clustered tags of past papers, is proposed in this paper. For performance evaluation of the proposed algorithm, our algorithm is compared with image search result of Flickr, typical tag based site, and is evaluated in accuracy and recall factor.

• Weed & Turfgrass Science
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• v.3 no.1
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• pp.50-55
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• 2014

This study is aimed at classifying the syntaxa of Ceratopteris thalictroides dominant community on the Nakdong River, and to collect basic data for research of habitat. The communities were carried out by using the Z.-M. School's method and numerical classification technique. The result of syntaxa was classified three communities such as Persicaria japonica-Ceratopteris thalictroides community, Lindernia procumbens-Ceratropteris thalictroides community, and Limnophila indica-Ceratopteris thalictroides community. The ordination analysis displayed the vegetation types with respect to complex environmental gradients. After ordination and clustering analysis, the effective humidity, soil stability, trampling effects, anthropogenic effects and flooding frequency were identified as the important factors deciding the vegetation pattern. It was pointed out to establish a long-term ecological site for protecting such vulnerable vegetation against overexploitation and global climate change.

• Proceedings of the Korea Crystallographic Association Conference
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• 2002.11a
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• pp.4-4
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• 2002

PDZ domains bind to short segments within target proteins in a sequence-specific fashion. GRIP/ABP family proteins contain six to seven PDZ domains and interact via its sixth PDZ domain (class Ⅱ) with the C-termini of various proteins, including liprin-α. In addition the PDZ456 domain mediates the formation of homo- and heteromultimers of GRIP proteins. To better understand the structural basis of peptide recognition by a class Ⅱ PDZ domain and DZ-mediated multimerization, we determined the crystal structures of the GRIPI PDZ6 domain, alone and in complex with a synthetic C-terminal octapeptide of human liprin-α, at resolutions of 1.5 Å and 1.8 Å, respectively. Remarkably, unlike other class Ⅱ PDZ domains, Ile736 at αB5 rather than conserved Leu732 at αB1 makes a direct hydrophobic contact with the side chain of the Tyr at the -2 position of the ligand. Moreover, the peptide-bound structure of PDZ6 shows a slight reorientation of helix αB, indicating that the second hydrophobic pocket undergoes a conformational adaptation to accommodate the bulkiness of the Tyr's side chain, and forms an antiparallel dimer through an interface located at a site distal to the peptide-binding groove. This configuration may enable formation of GRIP multimers and efficient clustering of GRIP-binding proteins.

• Aerospace Engineering and Technology
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• v.12 no.1
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• pp.87-93
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• 2013

The conceptual design lunar lander demonstrator has been developed to use as a test bed for advanced spacecraft technologies and to test a prototype planetary lander capable of vertical takeoff and landing. Size of the lunar lander demonstrator is the same as that of lunar lander conceptually designed, however, the weight of lunar lander demonstrator is designed in 1/6 scale in consideration of gravity difference between moon and earth. The thruster clustering and virtual flight test were performed in the demonstrator fixed on the ground. The demonstrator ground test has been conducted for two months in the test site for the solid motor combustion of the Goheung Flight Center. The purposes of ground test of demonstrator are to demonstrate and verify essential electronics, propulsion system, control algorithm, embedded software, structure and system operation technologies before developing the flight model lander. This paper is described about the virtual flight test including test configuration, test aims and test facilities

• Journal of Microbiology and Biotechnology
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• v.30 no.9
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• pp.1355-1366
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• 2020

Sediment bacterial communities are critical to the biogeochemical cycle in river ecosystems, but our understanding of the relationship between sediment bacterial communities and their specific input streams in rivers remains insufficient. In this study, we analyzed the sediment bacterial community structure in a local river receiving discharge of urban domestic sewage by applying Illumina MiSeq high-throughput sequencing. The results showed that the bacterial communities of sediments samples of different pollution types had similar dominant phyla, mainly Proteobacteria, Actinobacteria, Chloroflexi and Firmicutes, but their relative abundances were different. Moreover, there were great differences at the genus level. For example, the genus Bacillus showed statistically significant differences in the hotel site. The clustering of bacterial communities at various sites and the dominant families (i.e., Nocardioidaceae, and Sphingomonadaceae) observed in the residential quarter differed from other sites. This result suggested that environmentally induced species sorting greatly influenced the sediment bacterial community composition. The bacterial co-occurrence patterns showed that the river bacteria had a nonrandom modular structure. Microbial taxonomy from the same module had strong ecological links (such as the nitrogenium cycle and degradation of organic pollutants). Additionally, PICRUSt metabolic inference analysis showed the most important function of river bacterial communities under the influence of different types of domestic sewage was metabolism (e.g., genes related to xenobiotic degradation predominated in residential quarter samples). In general, our results emphasize that the adaptive changes and interactions in the bacterial community structure of river sediment represent responses to different exogenous pollution sources.

• The Journal of Internal Korean Medicine
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• v.42 no.4
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• pp.628-644
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• 2021

Objectives: This study analyzed, through network analysis and data mining analysis, the relationship between herbs used in osteoporosis prescriptions, diversified the analysis of osteoporosis-related prescriptions, and analyzed the combination of herbs used in osteoporosis-related prescriptions. Methods: The prescriptions used in osteoporosis treatment and experiments were established by conducting a full survey of the papers published by the OASIS site. A database for osteoporosis-related prescriptions was established, herbs were extracted, and the frequency of frequent herbs and prescriptions were investigated using Excel (MS offices ver. 2013). Using the freeware R version 4.0.3 (2020-10-10), igraph, and arules package, network analysis was performed in the first second of prescription composition. Results: Among the osteoporosis-related prescriptions, the most studied prescriptions are as follows.: Yukmijihwang-tang (六味地黃湯) and Samul-tang (四物湯). In the osteoporosis prescription network, herbs with connection centrality, proximity centrality, mediation centrality, and eigenvector centrality appeared in the order of Rehmanniae Radix Preparata, Angelicae Gigantis Radix, Poria Sclerotium, Paeoniae Radix, and Glycyrrhizae Radix et Rhizoma. After extracting the herbal combination network, including the corresponding herbs, and clustering it, it can be divided into drugs of the Yukmijihwang-tang (六味地黃湯) series and the Samul-tang (四物湯). Conclusions: This study could assist researchers in diversifyingy formula analysis in future studies. Moreover, the herbal combination used in osteoporosis prescriptions could be used to search for osteoporosis prescriptions in other databases or to create a new prescription.

• The Korean Journal of Applied Statistics
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• v.33 no.6
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• pp.833-842
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• 2020

Global platforms such as Netflix, Amazon, and YouTube have developed a precise recommendation system based on various information from large set of customers and many of the items recommended here are leading to actual purchases. In this paper, a cluster analysis was conducted according to the attribute of the content, expecting that there would be a difference in user preferences according to the attribute of the recommended content. Gower distance was used for use regardless of the type of variables. In this paper, using the data of movie rating site 'The Movie Dataset', the users were grouped hierarchically and recommended movies based on genre, director and actor variables. To evaluate the recommended systems proposed, user group was divided into train set and test set to examine the precision. The results showed that proposed algorithms have far higher precision than UBCF.

• Journal of Korean Society on Water Environment
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• v.39 no.4
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• pp.316-328
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• 2023

In this study, the characteristics of nonpoint pollutant outflow and contribution rate of pollution in Songya-stream mainstream and tributaries were analyzed. Further, water pollution management and improvement measures for pollution-oriented rivers were proposed. An on-site investigation was conducted to determine the inflow of major pollutants into the basin, and it was found that pollutants generated from agricultural land and livestock facilities flowed into the river, resulting in a high concentration of turbid water. Based on the analysis results of the pollution load data calculated through actual measurement monitoring (flow and water quality) and the occurrence and emission load data calculated using the national pollution source survey data, the S3 and S6 were selected as the concerned pollution tributaries in the Songya-stream basin. Results of cluster analysis using Pearson correlation coefficient evaluation and Density based spatial clustering of applications with noise (DBSCAN) technique showed that the S3 and S6 were most consistent with the C2 cluster (a cluster of Songya-stream mainstream owned area) corresponding to the mainstream of Songya-stream. The analysis results of the major pollutants in the concerned pollution tributaries showed that livestock and land pollutants were the major pollutants. Consequently, optimal management techniques such as fertilizer management, water gate management in paddy, vegetated filter strip and livestock manure public treatment were proposed to reduce livestock and land pollutants.

• Clinical and Experimental Vaccine Research
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• v.13 no.2
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• pp.155-165
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• 2024

Purpose: Pertussis bacteria have many pathogenic and virulent antigens and severe adverse reactions have occurred when using inactivated whole-cell pertussis vaccines. Therefore, inactivated acellular pertussis (aP) vaccines and genetically detoxified recombinant pertussis (rP) vaccines are being developed. The aim of this study was to assess the safety profile of a novel rP vaccine under development in comparison to commercial diphtheria-tetanus-acellular pertussis (DTaP) vaccines. Materials and Methods: The two positive control DTaP vaccines (two- and tri-components aP vaccines) and two experimental recombinant DTaP (rDTaP) vaccine (two- and tri-components aP vaccines adsorbed to either aluminum hydroxide or purified oat beta-glucan) were used. Temperature histamine sensitization test (HIST), indirect Chinese hamster ovary (CHO) cell cluster assay, mouse-weight-gain (MWG) test, leukocytosis promoting (LP) test, and intramuscular inflammatory cytokine assay of the injection site performed for safety assessments. Results: HIST results showed absence of residual pertussis toxin (PTx) in both control and experimental DTaP vaccine groups, whereas in groups immunized with tri-components vaccines, the experimental tri-components rDTaP absorbed to alum showed an ultra-small amount of 0.0066 IU/mL. CHO cell clustering was observed from 4 IU/mL in all groups. LP tests showed that neutrophils and lymphocytes were in the normal range in all groups immunized with the two components vaccine. However, in the tri-components control DTaP vaccine group, as well as two- and tri-components rDTaP with beta-glucan group, a higher monocyte count was observed 3 days after vaccination, although less than 2 times the normal range. In the MWG test, both groups showed changes less than 20% in body temperature and body weight before the after the final immunizations. Inflammatory cytokines within the muscle at the injection site on day 3 after intramuscular injection revealed no significant response in all groups. Conclusion: There were no findings associated with residual PTx, and no significant differences in both local and systemic adverse reactions in the novel rDTaP vaccine compared to existing available DTaP vaccines. The results suggest that the novel rDTaP vaccine is safe.