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http://dx.doi.org/10.11110/kjpt.2014.44.4.250

Genetic relationship of Aloe vera 'Saengjang', a new forma, based on cpDNA and ITS sequence variation  

Srikanth, Krishnamoorthy (Division of Science Education, Chonbuk National University)
Jang, Seon Il (Department of Health & Science, Jeonju University)
Whang, Sung Soo (Division of Science Education, Chonbuk National University)
Publication Information
Korean Journal of Plant Taxonomy / v.44, no.4, 2014 , pp. 250-256 More about this Journal
Abstract
This study was carried out to understand the genetic relationship of three Aloe spp. cultivated in Korea, A. saponaria, A. vera and A. arborescens and a new variant in Korea based on three plastid (matK, trnL-F, rbcL) and one nuclear (ITS regions) DNA barcode markers. A total of 2,420 bp sequence was amplified. Two indels were detected in the trnL region, and also several species specific nucleotide loci were detected in all 29 parsimonious informative sites, and 148 variable sites were detected among four taxa studied while 170 variable and 75 parsimonious sites were detected when other Aloe spp. in worldwide were used. An UPGMA phenogram with 10,000 bootstrap replication showed that the new variant was closest to A. vera. The variant was not morphologically and genetically concurrent with any reported species so far. The clustering of Aloe species were broadly in agreement with previously reported results.
Keywords
Aloe vera 'Saengjang'; ITS & cpDNA regions; genetic relationship;
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