• Title/Summary/Keyword: Single sequence

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An Assembly Sequence Planning of a Chip Mounter Using Transportation Algorithm (수송알고리즘에 의한 칩마운터의 조립순서계획)

  • Park, Tae-Hyung;Kim, Cheol-Han
    • Journal of Institute of Control, Robotics and Systems
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    • v.6 no.9
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    • pp.836-843
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    • 2000
  • A sequence planning method is proposed to reduce the assembly time of gantey-type chip mounters with single head. The overall path of the chip mounter is divided into forward and backward path, and formulate the optimization problem is formulated as an transpoetation problem and an Euler's tour problem. The transportation alforithm is applied to find optimal backward path, and Euler's tour algorithm used to generate an assembly sequence. Simulation results are presented to verify the usefulness of the proposed method.

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A Fault Section Detection Method for Ungrounded System Based on Phase Angle Comparison of Zero-Sequence Current (비접지 배전계통에서 영상전류 위상 비교에 의한 고장구간 검출 방법)

  • Yang, Xia;Choi, Myeon-Song;Lee, Seung-Jae
    • Proceedings of the KIEE Conference
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    • 2007.07a
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    • pp.31-32
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    • 2007
  • In this paper, a fault section detection method is proposed for ungrounded system in the case of a single line-to-ground fault. A conventional method is used for faulted feeder selection according to the angular relationship between zero-sequence currents of the feeders and zero-sequence voltage of the system. Fault section detection is based on the comparison of phase angle of zero-sequence current. Proposed method has been testified in a demo system by Matlab/Simulink simulations. Based on Distribution Automation System(DAS), Feeder Remote Terminal Unit(FRTU) is used to collect those necessary data, at present a demo system is under developing using Manufacturing Message Specification (MMS) in IEC61850 standard.

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Video Haze Removal Method in HLS Color Space (HLS 색상 공간에서 동영상의 안개제거 기법)

  • An, Jae Won;Ko, Yun-Ho
    • Journal of Korea Multimedia Society
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    • v.20 no.1
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    • pp.32-42
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    • 2017
  • This paper proposes a new haze removal method for moving image sequence. Since the conventional dark channel prior haze removal method adjusts each color component separately in RGB color space, there can be severe color distortion in the haze removed output image. In order to resolve this problem, this paper proposes a new haze removal scheme that adjusts luminance and saturation components in HLS color space while retaining hue component. Also the conventional dark channel prior haze removal method is developed to obtain best haze removal performance for a single image. Therefore, if it is applied to a moving image sequence, the estimated parameter values change rapidly and the haze removed output image sequence shows unnatural glitter defects. To overcome this problem, a new parameter estimation method using Kalman filter is proposed for moving image sequence. Experimental results demonstrate that the haze removal performance of the proposed method is better than that of the conventional dark channel prior method.

Complete genome sequence of Paenibacillus konkukensis sp. nov. SK3146 as a potential probiotic strain

  • Jung, Hae-In;Park, Sungkwon;Niu, Kai-Min;Lee, Sang-Won;Kothari, Damini;Yi, Kwon Jung;Kim, Soo-Ki
    • Journal of Animal Science and Technology
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    • v.63 no.3
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    • pp.666-670
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    • 2021
  • Paenibacillus konkukensis sp. nov., SK3146 is a novel strain isolated from a pig feed. Here, we present complete genome sequence of SK3146. The genome consists of a single circular genome measuring 7,968,964 bp in size with an average guanine + cytosine (G+C) content of 53.4%. Genomic annotation revealed that the strain encodes 151 proteins related to hydrolases (EC3), which was higher than those in Bacillus subtilis and Escherichia coli. Diverse kinds of hydrolases including galactosidase, glucosidase, cellulase, lipase, xylanase, and protease were found in the genome of SK3146, coupled with one bacteriocin encoding gene. The complete genome sequence of P. konkukensis SK3146 indicates the immense probiotic potential of the strain with nutrient digestibility and antimicrobial activity functions.

Complete chloroplast genome sequence of Clematis calcicola (Ranunculaceae), a species endemic to Korea

  • Beom Kyun PARK;Young-Jong JANG;Dong Chan SON;Hee-Young GIL;Sang-Chul KIM
    • Korean Journal of Plant Taxonomy
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    • v.52 no.4
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    • pp.262-268
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    • 2022
  • The complete chloroplast genome (cp genome) sequence of Clematis calcicola J. S. Kim (Ranunculaceae) is 159,655 bp in length. It consists of large (79,451 bp) and small (18,126 bp) single-copy regions and a pair of identical inverted repeats (31,039 bp). The genome contains 92 protein-coding genes, 36 transfer RNA genes, eight ribosomal RNA genes, and two pseudogenes. A phylogenetic analysis based on the cp genome of 19 taxa showed high similarity between our cp genome and data published for C. calcicola, which is recognized as a species endemic to the Korean Peninsula. The complete cp genome sequence of C. calcicola reported here provides important information for future phylogenetic and evolutionary studies of Ranunculaceae.

Procaryotic Expression of Porcine Acid-Labile Subunit of the 150-kDa Insulin-like Growth Factor Complex (미생물에서 돼지 150-kDa Insulin-Like Growth Factor Complex의 Acid-Labile Subunit 발현)

  • Lee, C. Young;Kang, Hye-Kyeong;Moon, Yang-Soo
    • Journal of Animal Science and Technology
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    • v.50 no.2
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    • pp.177-184
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    • 2008
  • Acid-labile subunit(ALS) is a 85-kDa glycosylated plasma protein which forms a 150-kDa ternary complex with 7.5-kDa insulin-like growth factor(IGF) and 40~45-kDa IGF-binding protein-3. In a previous study, the present authors prepared a porcine ALS(pALS) expression construct by inserting a pALS coding sequence into a plasmid vector following synthesis of the sequence by reverse transcription-polymerase chain reaction(RT-PCR). The expression construct, however, was subsequently found to have a mis-sense mutation at two bases of the pALS coding sequence which is presumed to have occurred through a PCR error. In the present study, the correct coding sequence was synthesized by the site-directed mutagenesis and inserted into the pET-28a(+) plasmid expression vector containing the His-tag sequence flanking the last codon of the insert DNA. After induction of the expression construct in E. coli BL21(DE3) cells, the resulting presumptive recombinant peptide was purified by the Ni-affinity chromatography. Upon SDS- PAGE, the affinity-purified peptide was resolved as a single band at a 66-kDa position which is consistent with the expected molecular mass of the presumptive recombinant pALS. Collectively, results indicate that a recombinant pALS peptide was successfully expressed and purified in the present study.

Study on a Noble Methodology for the Automatic Decision of Optimal Launch Angle Sequence under Multi-Target Engagement (다수 표적 연속교전 상황에서의 최적 발사각 Sequence 결정 개념 연구)

  • Ryu, Sunmee
    • Journal of the Korea Society for Simulation
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    • v.25 no.3
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    • pp.133-146
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    • 2016
  • To engage multiple missiles in single launcher against multiple targets, launcher system has to operate for optimized launch angle to each target sequentially. If the launch angle sequence is simply defined according to the target assignment order only, overall engagement time would be increased, and even in some engagement scenarios, it could be possible to miss some moving targets being out of proper engagement area. Therefore, the study on methodology for a real-time decision of optimized launch angle sequence is necessary. In this paper, the automatic decision model of launch angle sequence was suggested to minimize total engagement time by analyzing the simulation results of all engagement sequence set for multiple moving target scenario. Performance of proposed methodology for decision of optimal launch angle sequence was verified by comparing with the optimal or suboptimal sequence obtained from simulation results.

Replication and packaging of Turnip yellow mosaic virus RNA containing Flock house virus RNA1 sequence

  • Kim, Hui-Bae;Kim, Do-Yeong;Cho, Tae-Ju
    • BMB Reports
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    • v.47 no.6
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    • pp.330-335
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    • 2014
  • Turnip yellow mosaic virus (TYMV) is a spherical plant virus that has a single 6.3 kb positive strand RNA as a genome. In this study, RNA1 sequence of Flock house virus (FHV) was inserted into the TYMV genome to test whether TYMV can accommodate and express another viral entity. In the resulting construct, designated TY-FHV, the FHV RNA1 sequence was expressed as a TYMV subgenomic RNA. Northern analysis of the Nicotiana benthamiana leaves agroinfiltrated with the TY-FHV showed that both genomic and subgenomic FHV RNAs were abundantly produced. This indicates that the FHV RNA1 sequence was correctly expressed and translated to produce a functional FHV replicase. Although these FHV RNAs were not encapsidated, the FHV RNA having a TYMV CP sequence at the 3'-end was efficiently encapsidated. When an eGFP gene was inserted into the B2 ORF of the FHV sequence, a fusion protein of B2-eGFP was produced as expected.

A comparative analysis on Blind Adaptation Algorithms performances for User Detection in CDMA Systems (CDMA System에서 사용자 검파를 위한 Blind 적용 알고리즘에 관한 성능 비교 분석)

  • 조미령;윤석하
    • Journal of the Korea Computer Industry Society
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    • v.2 no.4
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    • pp.537-546
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    • 2001
  • Griffth's and LCCMA which are Single-user detection adaptive algorithm are proposed for mitigate MAI(multiple access interference) and the near-far problem in direct-sequence spread-spectrum CDMA system and MOE Algorithm is proposed for MMSE(Minimum Mean-Square Error). This paper pertains to three types of Blind adaptive algorithms which can upgrade system functionality without the requirements from training sequence. It goes further to compare and analyze the functionalities of the algorithms as per number of interfering users or data update rate of the users. The simulation results was that LCCMA algorithm was superior to other algorithms in both areas. Blind application enabled a more flexible network design by eliminating the necessity of training sequence.

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Nucleotide Sequence Analysis of the RNA-dependent RNA Polymerase Gene of Infectious Pancreatic Necrosis Virus DRT Strain

  • Lee, Hyung-Hoan;Chung, Hye-Kyung;Lee, Seong-Hun
    • Journal of Microbiology and Biotechnology
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    • v.4 no.4
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    • pp.264-269
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    • 1994
  • To determine the nucleotide sequence of the ds RNA segment B containing the RNA dependent RNA polymerase (RdRp) gene of the DRT strain of infectious pancreatic necrosis virus (lPNV), the cDNA of the ds RNA segment B of the DRT strain of IPNV was synthesized using the reverse transcriptase (RT)-polymerase chain reaction (PCR) and its cDNA nucleotide sequence was determined. The DRT segment B was 2, 783 bp long and contained only a single long open reading frame (ORF) of 2, 535 bp in length. This ORF nucleotides encoded the VPl protein, the putative RdRp of IPNV. The VPl protein comsisted of 845 amino acids. The molecular weight of the RdRp, as deduced from the nucleotide sequence, is 94, 426. The nucleotide sequence of the ORF of the DRT showed 89.7% homology to the Jasper strain, but 80.8% to the Sp strain. The amino acid sequence of the ORF of the DRT sho.wed 97.6% homology to the Jasper strain, but 88.7% to the Sp strain. The conserved GTP-binding motif was detected in VPl protein.

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