Browse > Article
http://dx.doi.org/10.5483/BMBRep.2014.47.6.187

Replication and packaging of Turnip yellow mosaic virus RNA containing Flock house virus RNA1 sequence  

Kim, Hui-Bae (Department of Biochemistry, Chungbuk National University)
Kim, Do-Yeong (Department of Biochemistry, Chungbuk National University)
Cho, Tae-Ju (Department of Biochemistry, Chungbuk National University)
Publication Information
BMB Reports / v.47, no.6, 2014 , pp. 330-335 More about this Journal
Abstract
Turnip yellow mosaic virus (TYMV) is a spherical plant virus that has a single 6.3 kb positive strand RNA as a genome. In this study, RNA1 sequence of Flock house virus (FHV) was inserted into the TYMV genome to test whether TYMV can accommodate and express another viral entity. In the resulting construct, designated TY-FHV, the FHV RNA1 sequence was expressed as a TYMV subgenomic RNA. Northern analysis of the Nicotiana benthamiana leaves agroinfiltrated with the TY-FHV showed that both genomic and subgenomic FHV RNAs were abundantly produced. This indicates that the FHV RNA1 sequence was correctly expressed and translated to produce a functional FHV replicase. Although these FHV RNAs were not encapsidated, the FHV RNA having a TYMV CP sequence at the 3'-end was efficiently encapsidated. When an eGFP gene was inserted into the B2 ORF of the FHV sequence, a fusion protein of B2-eGFP was produced as expected.
Keywords
Encapsidation; FHV RNA1; Flock house virus; Recombinant virus; TYMV;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Shin, H.-I., Kim, H. Y. and Cho, T.-J. (2010) The Pro/Hel Region is indispensable for packaging non-replicating Turnip Yellow Mosaic Virus RNA, but not replicating viral RNA. Mol. Cells 29, 463-469.   과학기술학회마을   DOI   ScienceOn
2 Miller, D. J., Schwartz, M. D. and Ahlquist, P. (2001) Flock house virus RNA replicates on outer mitochondrial membranes in Drosophila cells. J. Virol. 75, 11664-11676.   DOI   ScienceOn
3 Cho, T.-J. and Dreher, T. W. (2006) Encapsidation of genomic but not subgenomic Turnip yellow mosaic virus RNA by coat protein provided in trans. Virology 356, 126-135.   DOI   ScienceOn
4 Shin, H.-I., Chae, K.-H. and Cho, T.-J. (2013) Modification of Turnip yellow mosaic virus coat protein and its effect on virion assembly. BMB Rep. 46, 495-500.   과학기술학회마을   DOI   ScienceOn
5 Shin, S. Y., Kim, C. G. and Lee, Y. H. (2013) Egr-1 regulates the transcription of the BRCA1 gene by etoposide. BMB Rep. 46, 92-96.   과학기술학회마을   DOI   ScienceOn
6 Prod'homme, D., Jakubiec, A. Tournier, V., Drugeon, G. and Jupin, I. (2003) Targeting of the Turnip Yellow Mosaic Virus 66K replication protein to the chloroplast envelope is mediated by the 140K protein. J. Virol. 77, 9124-9135.   DOI   ScienceOn
7 Chen, J., Li, W. X., Xie, D., Peng, J.R. and Ding, S.W. (2004) Viral virulence protein suppresses RNA silencing-mediated defense but upregulates the role of microRNA in host gene expression. Plant Cell 16, 1302-1313.   DOI   ScienceOn
8 Yildiz, I., Shukla, S. and Steinmetz, N. F. (2011) Applications of viral nanoparticles in medicine. Curr. Opin. Biotechnol. 22, 901-908.   DOI   ScienceOn
9 Shin, H.-I., Kim, I.-C. and Cho, T.-J. (2008) Replication and encapsidation of recombinant Turnip yellow mosaic virus RNA. BMB Rep. 41, 739-744.   과학기술학회마을   DOI   ScienceOn
10 Ball, L. A. (1995) Requirements for the self-directed replication of Flock house virus RNA1. J. Virol. 69, 720-727.
11 Li, H., Li, W. X. and Ding, S. W. (2002) Induction and suppression of RNA silencing by an animal virus. Science 296, 1319-1321.   DOI   ScienceOn
12 Selling, B. H., Allison, R. F. and Kaesberg, P. (1990) Genomic RNA of an insect virus directs synthesis of infectious virions in plants. Proc. Natl. Acad. Sci. 87, 434-438.   DOI
13 Price, B. D., Ruekert, R. R. M. and Ahlquist, P. (1996) Complete replication of an animal virus and maintenance of expression vectors derived from it in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. 93, 9465-9470.   DOI   ScienceOn
14 Annamalai, P., Rofail, F., DeMason, D.A. and Rao, A.L.N. (2008) Replication-coupled packaging mechanism in positive-strand RNA viruses: Synchronized coexpression of functional multigenome RNA components of an animal and a plant virus in Nicotiana benthamiana cells by agroinfiltraion. J. Virol. 82, 1484-1495.   DOI   ScienceOn
15 Johnson, K. L. and Ball, L. A. (1999) Induction and maintenance of autonomous Flock house virus RNA1 replication. J. Virol. 73, 7933-7942.
16 Perrotta, A. T., Shih, I. and Been, M. D. (1999) Imidazole rescue of a cytosine mutation in a self-cleaving ribozyme. Science 286, 123-126.   DOI   ScienceOn
17 Schirawski, J., Voyatzakis, A., Zaccomer, B., Bernardi, F. and Haenni, A. L. (2000) Identification and functional analysis of the turnip yellow mosaic tymovirus subgenomic promoter. J. Virol. 74, 11073-11080.   DOI
18 Dreher, T. W. (2004) Turnip yellow mosaic virus: transfer RNA mimicry, chloroplasts and a C-rich genome. Mol. Plant Pathol. 5, 367-375.   DOI   ScienceOn
19 Price, B. D., Roeder, M. and Ahlquist, P. (2000) DNA-directed expression of functional flock house virus RNA1 derivatives in Saccharomyces cerevisiae, heterologous gene expression, and selective effects on subgenomic mRNA synthesis. J. Virol. 74, 11724-11733.   DOI