• Journal of Microbiology and Biotechnology
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• v.20 no.10
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• pp.1403-1414
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• 2010

Aspergillus awamori BTMFW032, isolated from sea water, produced tannase as an extracellular enzyme under submerged culture conditions. Enzymes with a specific activity of 2,761.89 IU/mg protein, a final yield of 0.51%, and a purification fold of 6.32 were obtained after purification through to homogeneity, by ultrafiltration and gel filtration. SDS-PAGE analyses, under nonreducing and reducing conditions, yielded a single band of 230 kDa and 37.8 kDa, respectively, indicating the presence of six identical monomers. A pI of 4.4 and a carbohydrate content of 8.02% were observed in the enzyme. The optimal temperature was found to be $30^{\circ}C$, although the enzyme was active in the range of $5-80^{\circ}C$. Two pH optima, pH 2 and pH 8, were recorded, although the enzyme was instable at a pH of 8, but stable at a pH of 2.0 for 24 h. Methylgallate recorded maximal affinity, and $K_m$ and $V_{max}$ were recorded at $1.9{\times}10^{-3}$M and 830 ${\mu}Mol$/min, respectively. The impacts of a number of metal salts, solvents, surfactants, and other typical enzyme inhibitors on tannase activity were determined in order to establish the novel characteristics of the enzyme. The gene encoding tannase, isolated from A. awamori, was found to be 1.232 kb, and nucleic acid sequence analysis revealed an open reading frame consisting of 1,122 bp (374 amino acids) of one stretch in the -1 strand. In silico analyses of gene sequences, and a comparison with reported sequences of other species of Aspergillus, indicate that the acidophilic tannase from marine A. awamori differs from that of other reported species.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.12 no.1
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• pp.275-281
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• 2012

Community network is a communication environment where heterogeneous devices can access and communicate with each other at any time and at any space to share information. To do so, mobile devices are required to be self-configured even in absence of communication infrastructures. Semi-infrastructured wireless ad-hoc network is a promising solution to meet with such a requirement. This paper proposes the VTC(virtual topology coordinator) system as an evaluation tool for examining network protocols that are intended to be deployed in the semi-infrastructured ad-hoc networks. VTC emulates multi-hops wireless network topology virtually using a mechanism of selective receiving MAC frame in a small area, where only a single hop communication is available. VTC system cannot consider all properties introduced in real wireless network, but do more wireless properties than verification through simulation.

• Journal of Korean Foot and Ankle Society
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• v.7 no.2
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• pp.151-156
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• 2003

Purpose: Proximal crescentic metatarsal osteotomy(PMO) is one of the most common procedures for correcting moderate to severe degree hallux valgus deformity. Although screw fixation is used for osteotomy site stability, loss of reduction can occur. The purpose of this study is to compare the sagittal plane stability of the conventional crescentic PMO fixed with a single screw with that of the crescentic PMO fixed with 1 screw and 2 supplemental K -wires. Material and Methods: Ten matched pairs of cadaveric foot specimens were used for the proximal crescentic metatarsal osteotomy. For one foot specimen of each pair, crescentic osteotomy was fixed with 4mm long threaded cannulated screw, while the matched pair was prepared by adding two axial 1.6mm K-wires to the conventionally fixed 4mm screw. The extensometer was used to measure the osteotomy gap as the metatarsal head was loaded continuously until failure using a servohydraulic MTS Mini Bionix test frame. The strength of fixation was normalized with the bone mineral density (BMD) of the paired specimen $(N{\times}cm^{2}/gm)$, Result: The average strength of the crescentic PMO with axial K-wire fixation ($458.8cm^{2}/gm$, S.D. 434.3) was significantly higher than the standard crescentic PMO ($367.5cm^{2}/gm$, S.D. 397,9) (p=0.05). Conclusion: Supplemental fixation with two axial K-wires can be added to the crescentic PMO to enhance the initial fixation stability to prevent the loss of reduction or dorsal malunion.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2013.10a
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• pp.1015-1017
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• 2013

Object tracking, in general, is a challenging problem. Difficulties in tracking objects can arise due to abrupt object motion, changing appearance patterns of both the object and the scene, non-rigid object structures, object-to-object and object-to-scene occlusions, and camera motion. Tracking is usually performed in the context of higher-level applications that require the location or the shape of the object in every frame. This paper describes an object tracking system based on active vision with two cameras, into algorithm of single camera tracking system an object active visual tracking and object locked system based on Extend Kalman Filter (EKF) is introduced, by analyzing data from which the next running state of the object can be figured out and after the tracking is performed at each of the cameras, the individual tracks are to be fused (combined) to obtain the final system object track.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.39C no.6
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• pp.503-511
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• 2014

In this paper, a novel, real-time video stitching algorithm in an H.264/AVC compressed domain is proposed. This enables viewers to watch multiple video contents using a single device. The basic concept of this paper is that the server is asked to combine multiple streams into one bit-stream based in a compressed domain. In other words, this paper presents a new compressed domain combiner that works in boundary macroblocks of input videos with re-calculating intra prediction mode, intra prediction MVD, a re-allocation of the coefficient table, and border extension methods. The rest of the macroblocks of the input video data are achieved simply by copying them. Simulation experiments have demonstrated the possibility and effectiveness of the proposed algorithm by showing that it is able to generate more than 103 frames per second, stitching four 480p-sized images into each frame.

• Computational Structural Engineering
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• v.10 no.4
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• pp.281-290
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• 1997

Current seismic design code is based on the assumption that the designed structures would be behaved inelastically during a severe earthquake ground motion. For this reason, seismic design forces calculated by seismic codes are much lower than the forces generated by design earthquakes which makes structures responding elastically. Present procedures for calculating seismic design forces are based on the use of elastic spectra reduced by a strength reduction factors known as "response modificaion factor". Because these factors were determined empirically, it is difficult to know how much inelastic behaviors of the structures exhibit. In this study, lateral strength required to maintain target ductility ratio was first calculated from nonlinear dynamic analysis of the single degree of freedom system. At the following step, base shear foeces specified in seismic design code compare with above results. If the base shear force required to maintain target ductility ratio was higher than the code specified one, the lack of required strength should be filled by overstrength and/or redundancy. Therefore, overstrength of moment resisting frame structure will be estimated from the results of push-over analysis.

• Proceedings of KOSOMES biannual meeting
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• 2007.05a
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• pp.169-174
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• 2007

The purpose in having a control rod an a buoy system is to control the motion of it. The system may be composed entirely af a single circular cylinder finder and a lang mooring anchor cable. A control rod has one function to perform in meeting its purpose, and that is to develop a control force in consequence of its orientation and movement relative to the water. The forces and moments generated as a result of the effects of mutual interference, then determine the stability characteristics of the body. In this paper, the study of control-rod-attached buoy's 2-dimensional section was accomplished. model tests and numerical simulations had been carried out with different diameters of control rods. and varying the Reynolds number $Re=5,000{\sim}25,000$ based an the cylinder diameter(D=50mm) to. predict the performance af the body and the 2 frame particle tracking method had been used to obtain the velocity distribution in the flaw field 50mm circular cylinder had been used during the whale experiments and measured results had been compared with each other.

• Journal of the Korea Society of Computer and Information
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• v.15 no.5
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• pp.57-65
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• 2010

In this paper, we propose a new real-time algorithm detecting the flame and smoke in digital CCTV images. Because the forest fire causes the enormous human life and damage of property, the early management according to the early sensing is very important. The proposed algorithm for monitoring forest fire is classified into the flame sensing and detection of smoke. The flame sensing algorithm detects a flame through the conditional test at YCbCr color model from the single frame. For the detection of smoke, firstly the background range is set by using differences between current picture and the average picture among the adjacent frames in the weighted value, and the pixels which get out of this range and have a gray-scale are detected in the smoke area. Because the proposed flame sensing algorithm is stronger than the existing algorithms in the change of the illuminance according to the quantity of sunshine, and the smoke detection algorithm senses the pixel of a gray-scale with the smoke considering the amount of change for unit time, the effective early forest fire detection is possible. The experimental results indicate that the proposed algorithm provides better performance than existing algorithms.

• Journal of the Earthquake Engineering Society of Korea
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• v.12 no.5
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• pp.23-29
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• 2008

This study investigates the dynamic instability of strength-limited bilinear single degree of freedom (SDF) systems under seismic excitation. The strength-limited bilinear hysteretic model best replicates the hysteretic behavior of the steel moment resisting frames. To estimate the dynamic instability of SDF systems, the collapse strength ratio is used, which is the yield-strength reduction factor when collapse occurs. Statistical studies are carried out to estimate median collapse strength ratios and those dispersions of strength-limited bilinear SDF systems with given natural periods, hardening stiffness ratios, post-capping stiffness ratios, ductility and damping ratios ranging from 2 to 20% subjected to 240 earthquake ground motions recorded on stiff soil sites. Equations to calculate median and standard deviation of collapse strength ratios in strength-limited bilinear SDF systems are obtained through nonlinear regression analysis. By using the proposed equations, this study estimated the probabilistic distribution of collapse strength ratios, and compared this with the exact values from which the accuracy of the proposed equations was verified.

• Journal of the Korean Society of Marine Environment & Safety
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• v.23 no.2
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• pp.200-207
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• 2017

The purpose in having a control surface on ships is to control the motion of the ship. The control surface may be composed entirely of a single movable surface or of a combination of fixed and movable portions. A control surface has one sole function to perform in meeting its purpose, and that is to develop a control force in consequence of its orientation and movement relative to the water. The forces and moments generated as a result of this rotation and angle of attack then determine the manoeuvring characteristics of the ship. In this paper, two-dimensional flow characteristics of a flapped rudder and a water-blowing control rudder were accomplished respectively by PIV method in a circulating water channel. Model test has been carried out with different angles of attack of main foil (NACA 0012) and flap's deflection angles to predict the performance of the flapped rudder and the water-blowing control rudder. The 2-frame particle tracking method has been used to obtain the velocity distribution in the flow field. $Re{\fallingdotseq}3.0{\times}10^4$ has been used during the whole experiments and measured results have been compared with each other.