• BMB Reports
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• 제38권5호
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• pp.526-532
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• 2005

A lectin with in-vitro anticancer activity against established human cancer cell lines has been purified by affinity chromatography on asialofetuin-linked amino activated silica beads from the tubers of Arisaema tortuosum, popularly known as Himalayan Cobra lily, a monocot plant from the family Araceae. The bound Arisaema tortuosum lectin (ATL) was eluted with glycine-HCl buffer, pH 2.5. ATL was effectively inhibited by asialofetuin, a complex desialylated serum glycoprotein as well as by N-acetyl-D-lactosamine, a disaccharide. It gave a single band corresponding to a subunit molecular weight of 13.5 kDa in SDS-PAGE, pH 8.8 both under reducing and non reducing conditions. When subjected to gel-filtration on Biogel P-200, it was found to have a molecular weight of 54 kDa, suggesting a homotetramer structure, in which individual polypeptides are not bound to each other with disulfide bonds. ATL is a glycoprotein with 0.9% carbohydrate content, stable up to $55^{\circ}C$ and at pH 2 to 10. The lectin had no requirement for divalent metal ions i.e. $Ca^{2+}$ and $Mn^{2+}$ for its activity. However, as reported for other monocot lectins, ATL gave multiple bands in isoelectric focusing and Native PAGE, pH 8.3. The lectin was found to inhibit in vitro proliferation of human cancer cell lines HT29, SiHa and OVCAR-5.

• 한국물환경학회지
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• 제24권6호
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• pp.682-689
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• 2008

The purpose of this study was to find out the variation between molecular size distribution (MSD) of natural organic matter (NOM) in raw waters after different water treatment processes like conventional process (coagulation, flocculation, filtration) followed by advanced oxidation process (ozonation, GAC adsorption). The MSD of NOM of Suji pilot plant were determined by Liquid Chromatography-Organic Carbon Detection (LC-OCD) which is a kine of high-performance size-exclusion chromatography (HPSEC) with nondispersive infrared (NDIR) detector and $UV_{254}$ detector. Five distinct fractions were generally separated from water samples with the Toyopearl HW-50S column, using 28 mmol phosphate buffer at pH 6.58 as an eluent. Large and intermediate humic fractions were the most dominant fractions in surface water. High molecular weight (HMW) matter was clearly easier to remove in coagulation and clarification than low molecular weight (LMW) matter. Water treatment processes removed the two largest fractions almost completely shifting the MSD towards smaller molecular size in DW. No more distinct variation of MSD was observed by ozone process after sand filtration but the SUVA value were obviously reduced during increase of the ozone doses. UVD results and HS-Diagram demonstrate that ozone induce not the variation of molecular size of humic substance but change the bond structure from aromatic rings or double bonds to single bond. Granular activated carbon (GAC) filtration removed 8~9% of organic compounds and showed better adsorption property for small MSD than large one.

• 한국HCI학회논문지
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• 제1권1호
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• pp.45-52
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• 2006

본 논문은 GPU를 활용한 이미지 공간 실시간 충돌 검사 기법을 설명한다. 닫힌 물체들이 충돌하지 않는 경우, 뷰잉 레이를 따라 물체의 앞면과 뒷면이 번갈아 가며 나타나는 것을 확인 할 수 있다. 그러나 물체 간 충돌이 일어나는 경우 이 현상이 깨어지게 된다. 이러한 특성에 기반하여 본 논문은 충돌 검사에 필요한 최소한의 표면 정보만 텍스쳐에 기록하여 충돌 검사를 수행하는 기법을 제안한다. 이 기법은 GPU의 framebuffer object 와 vertex buffer object, 그리고 occlusion query 등의 기능을 활용한다. 이러한 GPU의 기능을 이용하면 통상적인 이미지 기반 충돌검사에서 사용하는 multi-pass rendering 과 context switch 부하를 줄일 수 있다. 즉 기존의 이미지 기반 충돌 검사에 비해 적은 렌더링 횟수와 적은 렌더링 부하를 가진다. 본 논문에서 제안된 알고리즘은 변형체나 복잡한 물체에도 적용이 가능하며, 3D 게임이나 가상현실과 같은 실시간 어플리케이션에 적용될 수 있는 성능을 발휘한다.

• 한국지리정보학회지
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• 제11권2호
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• pp.1-12
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• 2008

버스와 지하철 등 대중교통수단은 승용차가 없는 시민들에게는 유일한 이동수단으로써 생활필수 서비스에 속한다. 또한 스마트성장을 비롯한 바람직한 도시성장유형들은 예외없이 대중교통수단 위주의 개발 (transit-oriented development)을 권장하고 있다. 그러므로 대중교통서비스는 모든 시민들에게 가용한 기본서비스가 되어야 함과 동시에 도시정책의 주요수단이 된다. 본 연구는 대중교통서비스 취약지역을 파악하고 그 분포특성을 분석함으로써 도시 대중교통정책 수립에 기여함을 목적으로 한다. 이를 위해 대구광역시를 사례지역으로 하여 통계청의 기초단위구 자료와 위성영상을 이용하여 분석단위를 세분화하고 이를 기준으로 버스 및 지하철 정류장과의 거리를 계산하였다. 그리고 정류장으로부터 300m이상 격리된 지역을 대중교통서비스 취약지역으로 간주하였다. 이 기준에 따라 대중교통서비스 취약지역의 특성을 면적, 인구, 주택유형 등의 측면에서 파악하였다. 분석결과 대중교통서비스는 지역별로 큰 차이가 나는 가운데 특히 교외지역과 농촌지역에서 취약계층이 많음이 입증되었다. 특히 본 연구는 이동성이 낮은 노인인구중 대중교통서비스를 이용하기 쉽지 않는 계층을 파악하고 공간적 분포를 제시하였다.

• Progress in Superconductivity
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• 제8권1호
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• pp.75-80
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• 2006

[ $Yb_2O_3$ ] films were successfully deposited on a cube-textured Ni and(100) $SrTiO_3$(STO) single crystal substrates by metal organic chemical vapor deposition(MOCVD) method using $H_2O$ vapor as an oxidant. $H_2O$ vapor was used in order to avoid the oxidation of Ni substrate. The working pressure and Ar flow rate were 10 Ton and 600 sccm, respectively. $Yb_2O_3$ films on STO were formed at high temperatures above $900^{\circ}C$. While XRD peaks from $Yb_2O_3$ were hardly detected at $900^{\circ}C$, the $Yb_2O_3$(400) texture was developed fur the films grown at deposition temperatures above $950^{\circ}C$. The AEM surface roughness of $Yb_2O_3$ film, grown on STO, was in the range of $6{\sim}10nm$ for the film deposited at $950^{\circ}C$ with a $H_2O$ vapor partial pressure of 5.5 Ton and deposition times of 3 and 5 mins. For cube-textured Ni substrate, both $Yb_2O_3$(222) and $Yb_2O_3$ (400) textures were developed textures at deposition temperatures above $850^{\circ}C$.

• 생명과학회지
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• 제27권10호
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• pp.1145-1151
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• 2017

세제에 의하여 대장균의 periplasm에서 penicillin G amidase (PGA)를 방출하는 방법을 연구하였다. 결과적으로 세제와 lysozyme의 혼합 작용이 효과적인 것으로 나타났다. 세포 투과성의 최적 조건을 알아보기 위하여 세제의 종류, 농도, pH, 반응 시간, 온도 등의 영향을 살펴보았다. 그리하여 대장균에서 재조합 PGA를 periplasm에서 방출하는 모델을 만들 수 있었고 방출된 PGA를 농축할 수 있었다. 실리카 구슬을 이용한 고정화 시스템으로 PGA 용액을 농축할 수 있었으며, 더 이상의 정제 과정 없이 순수하게 추출 할 수 있었다. 고정화된 PGA는 penicillin G 생성의 원료인 6-APA를 생산하는데 사용할 수 있었다. 이 방법은 대장균으로부터 재조합 단백질을 추출하는 간단한 방법이며 고정화 PGA를 이용하여 ${\beta}-lactam$ 항생물질의 산업적 생산 이용될 수 있을 것으로 사료된다.

• Journal of Microbiology and Biotechnology
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• 제23권7호
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• pp.942-952
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• 2013

Monoliths are functional porous materials with a three-dimensional continuous interconnected pore structure in a single piece. A monolith with uniform shape based on poly(${\gamma}$-glutamic acid) (PGA) has been prepared via a thermally induced phase separation technique using a mixture of dimethyl sulfoxide, water, and ethanol as solvent. The morphology of the obtained monolith was observed by scanning electron microscopy and the surface area of the monolith was evaluated by the Brunauer Emmett Teller method. The effects of fabrication parameters such as the concentration and molecular mass of PGA and the solvent composition have been systematically investigated. The PGA monolith was cross-linked with hexamethylene diisocyanate to produce the water-insoluble monolith. The addition of sodium chloride to the phase separation solvent affected the properties of the cross-linked monolith. The swelling ratio of the cross-linked monolith toward aqueous solutions depended on the buffer pH as well as the monolith fabrication condition. Copper(II) ion was efficiently adsorbed on the cross-linked PGA monolith, and the obtained copper-immobilized monolith showed strong antibacterial activity for Escherichia coli. By combination of the characteristic properties of PGA (e.g., high biocompatibility and biodegradability) and the unique features of monoliths (e.g., through-pore structure, large surface area, and high porosity with small pore size), the PGA monolith possesses large potentials for various industrial applications in the biomedical, environmental, analytical, and separation fields.

• Restorative Dentistry and Endodontics
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• 제41권4호
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• pp.246-254
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• 2016

Objectives: The aim of this investigation was to give insights into the impact of carbohydrate-electrolyte drinks on the likely capacity of enamel surface dissolution and the influence of human saliva exposure as a biological protective factor. Materials and Methods: The pH, titratable acidity (TA) to pH 7.0, and buffer capacity (${\beta}$) of common beverages ingested by patients under physical activity were analyzed. Then, we randomly distributed 50 specimens of human enamel into 5 groups. Processed and natural coconut water served as controls for testing three carbohydrate-electrolyte drinks. In all specimens, we measured surface microhardness (Knoop hardness numbers) and enamel loss (profilometry, ${\mu}m$) for baseline and after simulated intake cycling exposure model. We also prepared areas of specimens to be exposed to human saliva overnight prior to the simulated intake cycling exposure. The cycles were performed by alternated immersions in beverages and artificial saliva. ANOVA two-way and Tukey HDS tests were used. Results: The range of pH, TA, and ${\beta}$ were 2.85 - 4.81, 8.33 - 46.66 mM/L and 3.48 - $10.25mM/L{\times}pH$, respectively. The highest capacity of enamel surface dissolution was found for commercially available sports drinks for all variables. Single time human saliva exposure failed to significantly promote protective effect for the acidic attack of beverages. Conclusions: In this study, carbohydrate-electrolyte drinks usually consumed during endurance training may have a greater capacity of dissolution of enamel surface depending on their physicochemical proprieties associated with pH and titratable acidity.

• Archives of Pharmacal Research
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• 제27권6호
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• pp.662-669
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• 2004

The highly water-soluble monomethoxypoly(ethyleneglycol) (mPEG) prod rugs of cyciosporin A(CsA) were synthesized. These prod rugs were prepared by initially preparing intermediate in the form of carbonate at the 3'-positions of CsA with chloromethyl chloroformate, in the pres-ence of a base to provide a 3'-carbonated CsA intermediate. Reaction of the CsA intermediate with mPEG derivative in the presence of a base provides the desired water-soluble prod rugs. As a model, we chose molecular weight 5 kDa mPEG in the reaction with CsA to give water soluble prodrugs. To prove that the prod rug is decomposed in the body to produce CsA, the enzymatic hydrolysis test was conducted using human liver homogenate at $37^{\circ}C$. The prodrug was decomposed in human liver homogenate to produce the active material, CsA, and the hydrolysis half-life ($t_{1/2}$) of the prodrug, KI-306 was 2.2 minutes at $37^{\circ}C$. However, a demon-stration of non-enzymatic conversion in pH 7.4 phosphate buffer was provided by the fact that the half-life ($t_{1/2}$) is 21 hours at 37$^{\circ}C$. The hydrolysis test in rat whole blood was also conducted. The hydrolysis was seen with half-life ($t_{1/2}$) of about 9.9, 65.0, 14.2, 3.4, 2.1 9.5, and 1.6 minutes for KI-306, 309, 312, 313, 315, 316, and 317, respectively. This is the ideal for CsA prodrug. The pharmacokinetic study of the prodrug, KI-306, in comparison to the commer-cial product (Sandimmune Neoral Solution) was also carried out after single oral dose. Each rat received 7 mg／kg of CsA equivalent dose. Especially, the prodrug KI-306 exhibits higher AUC and $C_{max}$ than the conventional Neoral. The AUC and $C_{max}$ were increased nearly 1.5 fold. The kinetic value was also seen with $T_{max}$ of about 1.43 and 2.44 hours for KI-306 and Neoral, respectively.

• 대한전자공학회논문지SD
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• 제48권12호
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• pp.45-51
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• 2011

본 논문에서는 $0.13{\mu}m$ CMOS 공정을 사용하여 800MHz~5.8GHz 대역 내 다양한 무선통신 표준을 포함하는 광대역 저잡음 증폭기(wideband-LNA)를 구현하였다. 저잡음 특성을 개선하기 위하여 제작한 LNA는 두 단으로 구성되었으며, 입력캐스코드 단 및 잡음신호만을 상쇄시키는 출력 버퍼단으로 구성하였다. 또한, 피드백 저항을 이용함으로써, 광대역 임피던스 매칭 효과 및 넓은 대역폭을 구현하였다. 측정결과, 811MHz~5.8GHz의 주파수 응답과 대역폭 내에서 최대 11.7dB의 전력이득 및 2.58~5.11dB의 잡음지수(NF)를 얻었다. 제작한 칩은 $0.7{\times}0.9mm^2$의 면적을 가지며 1.2V의 전원전압에서 12mW의 낮은 전력을 소모 한다.