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Identification of sex-specific SNPS in burbot Lota lota using RAD sequencing: conservation and management applications

  • Vu, Ninh V.;Eardley, Daniel L.;Delomas, Thomas A.;Campbell, Matthew R.
    • Fisheries and Aquatic Sciences
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    • v.22 no.8
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    • pp.18.1-18.5
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    • 2019
  • The development of sex-specific genetic assays in a species provides both a method for identifying the system of sex determination and a valuable tool to address questions of conservation and management importance. In this study, we focused on the identification of single nucleotide polymorphisms (SNPs) that differentiate genetic sex in burbot Lota lota. Burbot are the only true freshwater representative of the cod family and a species of conservation and management importance throughout Eurasia and North America. To identify sex-specific SNPs, we utilized restriction site-associated DNA sequencing (RADseq) to interrogate thousands of SNPs in burbot samples of known phenotypic sex. We discovered 170,569 biallelic SNPs, none of which fit the pattern expected under female heterogamety. However, we identified 22 SNPs that fit the pattern expected under male heterogamety (males heterozygous XY, females fixed XX) and, from these, developed two genetic assays that robustly (~ 97% genotyping success) and accurately (> 99% correct) sexed burbot samples. These sex-specific genetic assays will benefit growing conservation aquaculture programs for this species and allow future assessments of sex-specific migration, growth, and mortality.

The Study on the Priority of First Person Shooter game Elements using Delphi Methodology (FPS게임 구성요소의 중요도 분석방법에 관한 연구 1 -델파이기법을 이용한 독립요소의 계층설계와 검증을 중심으로-)

  • Bae, Hye-Jin;Kim, Suk-Tae
    • Archives of design research
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    • v.20 no.3 s.71
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    • pp.61-72
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    • 2007
  • Having started with "Space War", the first game produced by MIT in the 1960's, the gaming industry expanded rapidly and grew to a large size over a short period of time: the brand new games being launched on the market are found to contain many different elements making up a single content in that it is often called the 'the most comprehensive ultimate fruits' of the design technologies. This also translates into a large increase in the number of things which need to be considered in developing games, complicating the plans on the financial budget, the work force, and the time to be committed. Therefore, an approach for analyzing the elements which make up a game, computing the importance of each of them, and assessing those games to be developed in the future, is the key to a successful development of games. Many decision-making activities are often required under such a planning process. The decision-making task involves many difficulties which are outlined as follows: the multi-factor problem; the uncertainty problem impeding the elements from being "quantified" the complex multi-purpose problem for which the outcome aims confusion among decision-makers and the problem with determining the priority order of multi-stages leading to the decision-making process. In this study we plan to suggest AHP (Analytic Hierarchy Process) so that these problems can be worked out comprehensively, and logical and rational alternative plan can be proposed through the quantification of the "uncertain" data. The analysis was conducted by taking FPS (First Person Shooting) which is currently dominating the gaming industry, as subjects for this study. The most important consideration in conducting AHP analysis is to accurately group the elements of the subjects to be analyzed objectively, and arrange them hierarchically, and to analyze the importance through pair-wise comparison between the elements. The study is composed of 2 parts of analyzing these elements and computing the importance between them, and choosing an alternative plan. Among these this paper is particularly focused on the Delphi technique-based objective element analyzing and hierarchy of the FPS games.

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A Study on Switching Intention of Broadcasting Service to MCN Service by Migration Theory (새로운 방송 서비스로의 이전 : 이주 이론을 통한 MCN으로 전환의도 연구)

  • Kim, Yonghee
    • The Journal of Industrial Distribution & Business
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    • v.10 no.1
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    • pp.59-67
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    • 2019
  • Purpose - The Millennial Generation, which grew in the wake of the spread of the Internet and rapid changes in the media environment, is rapidly moving from the traditional broadcasting environment to the Internet-broadcasting environment in terms of content acceptance. With the emergence of UGC (User-generated content), the change in the status of single-person content creators enables the growth of multi-channel networks (MCN), a new content-distribution platform and an agency concept for single creators. Youtube-based MCN produces multiple single star producers and casts and provides its own video series through Youtube. It is also emerging as a major M&A target for global media providers in terms of providing content to a wide range of consumers with the same interests and consumption characteristics. In addition, for the Millennials generation, which are part of their lives, MCN is becoming the most suitable media for TGIF (Twitter, Google, i-phone, Facebook). Accordingly, this study defines newly emerging MCNs and analyzes the factors for accepting MCN-produced content based on the push-pull-mooring (PPM) model. Research design, data, and methodology - An empirical analysis is performed through a questionnaire survey. For this purpose, 204 people who have experience of watching MCN were studied. Collected data is processed through analysis of a structural equation model using R to test the hypothesis. Results - For the MCN service to become an alternative to existing media, it is necessary to continuously promote cultural diversity and diversity of attempts that conventional media cannot provide. It is the attractiveness of the alternative that has the greatest influence on the intention to switch to a MCN service. When we look at MCN content so far, certain patterns such as game progress, introduction, food, and chat rooms have already appeared. We need to overcome this and develop a completely new conceptual content that we have never seen before. This requires a more generous viewer perception of the topics covered. For diversity, linguistic and verbal violence should be tolerant in common sense to provide a foundation for securing cultural diversity. Conclusions - In this study, we tried to develop a comprehensive approach to the substitution effect of MCN. In terms of academic achievement, the PPM model is used to enhance the utilization of media and broadcasting. Practical implications are to provide an analytical framework for verifying alternative or complementary effects when viewers switch to MCN.

Policy Modeling for Efficient Reinforcement Learning in Adversarial Multi-Agent Environments (적대적 멀티 에이전트 환경에서 효율적인 강화 학습을 위한 정책 모델링)

  • Kwon, Ki-Duk;Kim, In-Cheol
    • Journal of KIISE:Software and Applications
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    • v.35 no.3
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    • pp.179-188
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    • 2008
  • An important issue in multiagent reinforcement learning is how an agent should team its optimal policy through trial-and-error interactions in a dynamic environment where there exist other agents able to influence its own performance. Most previous works for multiagent reinforcement teaming tend to apply single-agent reinforcement learning techniques without any extensions or are based upon some unrealistic assumptions even though they build and use explicit models of other agents. In this paper, basic concepts that constitute the common foundation of multiagent reinforcement learning techniques are first formulated, and then, based on these concepts, previous works are compared in terms of characteristics and limitations. After that, a policy model of the opponent agent and a new multiagent reinforcement learning method using this model are introduced. Unlike previous works, the proposed multiagent reinforcement learning method utilize a policy model instead of the Q function model of the opponent agent. Moreover, this learning method can improve learning efficiency by using a simpler one than other richer but time-consuming policy models such as Finite State Machines(FSM) and Markov chains. In this paper. the Cat and Mouse game is introduced as an adversarial multiagent environment. And effectiveness of the proposed multiagent reinforcement learning method is analyzed through experiments using this game as testbed.

Hidden Markov Model for Gesture Recognition (제스처 인식을 위한 은닉 마르코프 모델)

  • Park, Hye-Sun;Kim, Eun-Yi;Kim, Hang-Joon
    • Journal of the Institute of Electronics Engineers of Korea CI
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    • v.43 no.1 s.307
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    • pp.17-26
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    • 2006
  • This paper proposes a novel hidden Markov model (HMM)-based gesture recognition method and applies it to an HCI to control a computer game. The novelty of the proposed method is two-fold: 1) the proposed method uses a continuous streaming of human motion as the input to the HMM instead of isolated data sequences or pre-segmented sequences of data and 2) the gesture segmentation and recognition are performed simultaneously. The proposed method consists of a single HMM composed of thirteen gesture-specific HMMs that independently recognize certain gestures. It takes a continuous stream of pose symbols as an input, where a pose is composed of coordinates that indicate the face, left hand, and right hand. Whenever a new input Pose arrives, the HMM continuously updates its state probabilities, then recognizes a gesture if the probability of a distinctive state exceeds a predefined threshold. To assess the validity of the proposed method, it was applied to a real game, Quake II, and the results demonstrated that the proposed HMM could provide very useful information to enhance the discrimination between different classes and reduce the computational cost.

Technology to create a 360-degree panorama of a square room using a single projector and a hemispherical mirror (1대의 프로젝터와 반구형 반사경을 이용한 사각방 360도 파노라마 생성 기법)

  • Lee, Jung-jik;Park, Yoen-yong;Lee, Yun-sang;Lee, Jun-yuep;Jung, Eun-yeong;Yu, Rim;Kang, Myongjin;Jung, Moon-ryul
    • Journal of the Korea Computer Graphics Society
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    • v.26 no.3
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    • pp.133-142
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    • 2020
  • In this research, we describe the method of implementing a 360-degree panorama using one projector, in terms of hardware and in the production of projected pre-distortion images. We propose a method of installing a projector and a reflector on the central ceiling of the space to minimize the shadows generated based on the position of the spectators. We used a virtual camera and virtual space where the projector and hemisphere positions were set to the same as in the exhibition space in Unity. After the image projected on the screen was mapped on the wall of the virtual space, the pre-distortion image was created by the method of capturing from the virtual camera using the ray tracing technique. When the produced pre-distortion image is hemispherical reflected and projected by the projector installed at the same position as the virtual camera, the image is reflected and projected 360 degrees on the panoramic screen.

A Study on the Continuous Speech Recognition for the Automatic Creation of International Phonetics (국제 음소의 자동 생성을 활용한 연속음성인식에 관한 연구)

  • Kim, Suk-Dong;Hong, Seong-Soo;Shin, Chwa-Cheul;Woo, In-Sung;Kang, Heung-Soon
    • Journal of Korea Game Society
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    • v.7 no.2
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    • pp.83-90
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    • 2007
  • One result of the trend towards globalization is an increased number of projects that focus on natural language processing. Automatic speech recognition (ASR) technologies, for example, hold great promise in facilitating global communications and collaborations. Unfortunately, to date, most research projects focus on single widely spoken languages. Therefore, the cost to adapt a particular ASR tool for use with other languages is often prohibitive. This work takes a more general approach. We propose an International Phoneticizing Engine (IPE) that interprets input files supplied in our Phonetic Language Identity (PLI) format to build a dictionary. IPE is language independent and rule based. It operates by decomposing the dictionary creation process into a set of well-defined steps. These steps reduce rule conflicts, allow for rule creation by people without linguistics training, and optimize run-time efficiency. Dictionaries created by the IPE can be used with the speech recognition system. IPE defines an easy-to-use systematic approach that can obtained 92.55% for the recognition rate of Korean speech and 89.93% for English.

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The Biomechanical Properties of the Shock Absorption Phase during Drop Landing According to Landing Types (드롭랜딩 시 착지형태에 따른 충격흡수구간의 운동역학적 특성)

  • Park, Gu-Tae;Yoo, Kyoung-Seok
    • Korean Journal of Applied Biomechanics
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    • v.25 no.1
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    • pp.29-37
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    • 2015
  • Objective : The purpose of this study was to investigate the biomechanical properties of shock absorption strategy and postural stability during the drop landing for each types. Methods : The motions were captured with Vicon Motion Capture System, with the fourteen infra-red cameras (100Hz) and synchronized with GRF(ground reaction force) data(1000Hz). Ten male soccer players performed a drop landing with single-leg and bi-legs on the 30cm height box. Dependent variables were the CoM trajectory and the Joint Moment. Statistical computations were performed using the paired t-test and ANOVA with Turkey HSD as post-hoc. Results : The dominant leg was confirmed to show a significant difference between the left leg and right leg as the inverted pendulum model during Drop Landing(Phase 1 & Phase 2). One-leg drop landing type had the higher CoM displacement, the peak of joint moment with the shock absorption than Bi-leg landing type. As a lower extremity joint kinetics analysis, the knee joint showed a function of shock absorption in the anterior-posterior, and the hip joint showed a function of the stability and shock absorption in the medial-lateral directions. Conclusion : These findings indicate that the instant equilibrium of posture balance(phase 1) was assessed by the passive phase as Class 1 leverage on the effect of the stability of shock absorption(phase 2) assessed by the active phase on the effect of Class 2 leverage. Application : This study shows that the cause of musculo-skeletal injuries estimated to be focused on the passive phase of landing and this findings could help the prevention of lower damage from loads involving landing related to the game of sports.

Study of a Policy Plan for Vitalizing a family Leisure (가족여가 활성화를 위한 정책방안)

  • Yoon, So-Young
    • Journal of Families and Better Life
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    • v.27 no.4
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    • pp.189-201
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    • 2009
  • This study is to solve following problems by a family leisure as the problem of a single person in seclusion type, teenager-children being addicted to game, the disconnection of culture inheritance among inter-generation, the elder being isolated from leisure, the increase of the children using a key to go into home alone, the problem of a gender discrimination. This study defines the policy for a family leisure as "policy that ultimately creates the happiness of family, prevents the family problem and deals with it, through the leisure activities that are suitable to family life cycle". It also considers some other important properties(equality, gender and family life cycle, space) besides the constitution and function of family leisure. To create the happiness of family, the direction of the policy for a family leisure heads toward the positive function such as unification, creativity, and psychological stability of family members. At this point, the object of the policy for a family leisure is a unit of the family and it focuses on the level of interaction and positive function development between family members, being beyond the concept of family activity. Therefore, the policy that is to vitalize a family leisure must contain the interaction and positive function between the family members. Hereupon, to vitalize a family leisure, this study leads above-mentioned subjects based on programming, running leisure facility, information system, experts, social awareness, and supporting to the various family. Specialty, the process of leading these subjects is connected to the suggestion of the concrete program and then, is to result in supporting the program development for the essential leisure policy.

A comparison of the forces on dome and prism for straight and tornadic wind using CFD model

  • Yousef, Majdi A.A.;Selvam, Panneer R.;Prakash, Jai
    • Wind and Structures
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    • v.26 no.6
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    • pp.369-382
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    • 2018
  • Tornadoes are vertical swirling air formed because of the existence of layers of air with contrasting features of temperature, wind flow, moisture, and density. Tornadoes induce completely different wind forces than a straight-line (SL) wind. A suitably designed building for an SL wind may fail when exposed to a tornado-wind of the same wind speed. It is necessary to design buildings that are more resistant to tornadoes. In tornado-damaged areas, dome buildings seem to have less damage. As a dome structure is naturally wind resistant, domes have been used in back yards, as single family homes, as in-law quarters, man caves, game rooms, storm shelters, etc. However, little attention has been paid to the tornadic wind interactions with dome buildings. In this work, the tornado forces on a dome are computed using Computational Fluid Dynamics (CFD) for tornadic and SL wind. Then, the interaction of a tornado with a dome and a prism building are compared and analyzed. This work describes the results of the tornado wind effect on dome and prism buildings. The conclusions drawn from this study are illustrated in visualizations. The tornado force coefficients on a dome building are larger than SL wind forces, about 120% more in x- and y-directions and 280% more in z-direction. The tornado maximum pressure coefficients are also higher than SL wind by 150%. The tornado force coefficients on the prism are larger than the forces on the dome, about 100% more in x- and y-directions, and about 180% more in z-direction. The tornado maximum pressure coefficients on prism also are greater those on dome by 150% more. Hence, a dome building has less tornadic load than a prism because of its aerodynamic shape.