• Nuclear Medicine and Molecular Imaging
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• v.43 no.5
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• pp.468-477
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• 2009

Purpose: Hydrodynamic-based procedure is a simple and effective gene delivery method to lead a high gene expression in liver tissue. Non-invasive imaging reporter gene system has been used widely with herpes simplex virus type 1 thymidine kinase (HSV1-tk) and its various substrates. In the present study, we investigated to image the expression of HSV1-tk gene with 5-(2-iodovinyD-2'-deoxyuridine (IVDU) in mouse liver by the hydrodynamicbased procedure. Materials and Methods: HSV1-tk or enhanced green fluorescence protein (EGFP) encoded plasmid DNA was transferred into the mouse liver by hydrodynaminc injection. At 24 h post-injection, RT-PCR, biodistribution, fluorescence imaging, nuclear imaging and digital wholebody autoradiography (DWBA) were performed to confirm transferred gene expression. Results: In RT-PCR assay using mRNA from the mouse liver, specific bands of HSV1-tk and EGFP gene were observed in HSV1-tk and EGFP expressing plasmid injected mouse, respectively. Higher uptake of radiolabeled IVDU was exhibited in liver of HSV1-tk gene transferred mouse by biodistribution study. In fluorescence imaging, the liver showed specific fluorescence signal in EGFP gene transferred mouse. Gamma-camera image and DWBA results showed that radiolabeled IVDU was accumulated in the liver of HSV1-tk gene transferred mouse. Conclusion: In this study, hydrodynamic-based procedure was effective in liver-specific gene delivery and it could be quantified with molecular imaging methods. Therefore, co-expression of HSV1-tk reporter gene and target gene by hydrodynamic-based procedure is expected to be a useful method for the evaluation of the target gene expression level with radiolabeled IVDU.

• Journal of Plant Biotechnology
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• v.47 no.4
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• pp.309-315
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• 2020

Advances in biotechnology have led to progress in crop genetic engineering to improve agricultural productivity. The use of genetically modified (GM) crops has increased, as have consumers' and regulators' concerns about the safety of GM crops to human health, and ecological biodiversity. As such, the identification of GM crops is a critical issue for developers and distributors, and their labeling is mandatory. Multiplex polymerase chain reaction (PCR) has been developed and its use validated for the detection and identification of GM crops in quarantine. Herein, we established a simultaneous detection method to identify four GM maize events. Event-specific primers were designed between the junction region of transgene and genome of four GM maize lines, namely 5307, DAS-40278-9, MON87460, and MON87427. To verify the efficiency and accuracy of the multiplex PCR we used specificity analysis, limit of detection evaluation, and mixed certified reference materials identification. The multiplex PCR method was applied to analyze 29 living, modified maize volunteers collected in South Korea in 2018 and 2019. We performed multiplex PCR analysis to identify events and confirmed the result by simplex PCR using each event-specific primer. As a result, rather than detecting each event individually, the simultaneous detection PCR method enabled the rapid analysis of 29 GM maize volunteers. Thus, the novel multiplex PCR method is applicable for living modified organism volunteer identification.

• The Journal of the Korea Contents Association
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• v.9 no.10
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• pp.22-31
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• 2009

Although the amount of UCC(user created content) rapidly grows nowadays, there are various problems in authoring, distribution and management of this abundant content because the platforms for fabrication and replaying content are not compatible and the authoring environments are not sufficiently interoperable with other site or with each other. In this paper, we propose a CPMS(content publication and management system) scheme improved in interoperability to solve these problems, and we design a novel system enhancing the efficiency and speed of content distribution and management by minimizing the repetitions in the processes of production, reshaping, and publication. Moreover the proposed system adopts an improved content management technology by changing from simple point-to-point method to the centralized distribution method, and as for the service scheme, we change from the site link method to the database link method, and we also take duplex interlink scheme instead of simplex one. We finally compare the proposed system to the conventional ones as points of view of compatibility and interoperability to prove the improved performance of our system.

• Journal of Digital Convergence
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• v.12 no.2
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• pp.257-262
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• 2014

Although the amount of UCC(user created content) rapidly grows nowadays, there are various problems in authoring, distribution and management of this abundant content because the platforms for fabrication and replaying content are not compatible and the authoring environments are not sufficiently interoperable with other site or with each other. In this paper, we propose a CMS(content management system) scheme improved in interoperability to solve these problems, and we design a novel system enhancing the efficiency and speed of content distribution and management by minimizing the repetitions in the processes of production, reshaping, and publication. Moreover the proposed system adopts an improved content management technology by changing from simple point-to-point method to the centralized distribution method, and as for the service scheme, we change from the site link method to the database link method, and we also take duplex interlink scheme instead of simplex one. We finally compare the proposed system to the conventional ones as points of view of compatibility and interoperability to prove the improved performance of our system.

• Korean Journal of Cognitive Science
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• v.31 no.1
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• pp.1-23
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• 2020

This paper introduces SNUDM, an analysis program for Ratcliff's diffusion model, which has been one of the most important models in cognitive psychology over the past 35 years and which has come to occupy an important place in cognitive neuroscience in recent years. The analysis tool is designed with the basic principles of easy comprehension and simplicity in use. A diffusion process was programmed as the limit of a simple random walk in a manner resembling Ratcliff & Tuerlinckx(2002). The response time distribution of the model was constructed by simulating the time taken by a random walk until it reaches a threshold with small steps. The optimal parameter values in the model are found to be the smallest value of the chi-square values obtained by comparing the resulting distribution and the experimental data using Simplex method. For simplicity and ease of use, the input file used here is created as a file containing the quantile of the reaction time, the trials and other information. The number of participants and the number of conditions required for such work programs are given in a way that answers the question. Using this analysis tool, the experimental data of Ratcliff, Gomez, & McKoon(2004) were analyzed. We found the very similar pattern of parameter values to Ratcliff et al.(2004) found. When comparing DMAT, fast-dm and SNUDM with the generated data, we found that when the number of trials is small, SNUDM estimates the boundary parameter to a value similar to fast-dm and less than the DMAT. In addition, when the number of trials was large, it was confirmed that all three tools estimate parameters similarly.

• Progress in Medical Physics
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• v.5 no.1
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• pp.41-53
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• 1994

The accurate localization of electrical sources in the brain is one of the most important questions in EEG, especially in the analysis of evoked responses and of epileptiform spike activity. A detailed simulation study of single dipole source estimation based on EEG is given in this paper. The effects of dipole model parameters on single dipole source tracing in EEG are examined in some detail using the Monte Carlo simulation. The error of source localization is found to be greatly influenced by how the electrodes are distributed over the head and the number of them.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.24 no.3 s.174
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• pp.661-666
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• 2000

Optimization of the elastic joint of train is performed according to the minimization of ten responses which represent driving safety and ride comfort of train and analyzed by using the each response se surface model from stochastic design of experiments. After the each response surface model is constructed, the main effect and sensitivity analyses are successfully performed by 2nd order approximated regression model as described in this paper. We can get the optimal solutions using by nonlinear programming method such as simplex or interval optimization algorithms. The response surface models and the optimization algorithms are used together to obtain the optimal design of the elastic joint of train. the ten 2nd order polynomial response surface models of the three translational stiffness of the elastic joint (design factors) are constructed by using CCD(Central Composite Design) and the multi-objective optimization is also performed by applying min-max and distance minimization techniques of relative target deviation.

• 한국전산유체공학회:학술대회논문집
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• 1999.11a
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• pp.77-84
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• 1999

Numerical design optimization techniques are implemented for the improvement of the ram accelerator performance. The design object is to find the minimum ram tube length required to accelerate projectile from initial velocity $V_0$ to target velocity $V_e$. The premixture is composed of $H_2,\;O_2,\;N_2$ and the mole numbers of these species are selected as design variables. The objective function and the constraints are linearized during the optimization process and gradient-based Simplex method and SLP(Sequential Linear Programming) have been employed. With the assumption of two dimensional inviscid flow for internal flow field, the analyses of the nonequilibrium chemical reactions for 8 steps 7 species lave been performed. To determined the tube length, ram tube internal flow field is assumed to be in a quasi-steady state and the flow velocity is divided into several subregions with equal interval. Hence the thrust coefficients and accelerations for corresponding subregions are obtained and integrated for the whole velocity region. With the proposed design optimization techniques, the total ram tube length had been reduced $19\%$ within 7 design iterations. This optimization procedure can be directly applied to the multi-stage, multi-premixture ram accelerator design optimization problems.

• Journal of Institute of Control, Robotics and Systems
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• v.6 no.7
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• pp.559-567
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• 2000

This paper proposes simple and practical methods in order to overcome complex formulation and heavy computational burden of the forward kinematics of 6 앨 3-6 type parallel manipulators. Three ap-proaches for the forward kinematics are presented : one extra sensor a modified structure and novel numerical method. The proposed methods are applied to the forward kinematics of a new 6 앨 parallel manipulator with special geometry that has three internal links three external links and a moving platform of a cone shape. The proposed methods use three tetrahedrons for finding the position and orientation vector of the moving platform. The main advantages of the appraches using tetrahedrons are to abbreviate the formulation to easily find so-lutions of the forward kinematics and to be able to practically control of the manipulator in real time.

• The Journal of Korean Obstetrics and Gynecology
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• v.31 no.1
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• pp.99-121
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• 2018

Objectives: Genital herpes is common disease in gynecological field. Although various treatment options such as herbal medicine, acupuncture, moxibustion are used in genital herpes, there is not enough evidence about the treatment options. This study is to prove the efficacy of oriental medicine on genital herpes by investigating papers and suggest direction of future research. Method: We searched for papers which had both genital herpes and oriental medicine from Cochrane, Pubmed, Scopus, CNKI, Oasis, Korean traditional knowledge portal, Journal of Korean Obstetrics & Gynecology up to November 2017. After searching papers, we classified according to the study design and analyzed selected studies. Results: Sixteen papers were finally selected. Four papers are laboratory studies with Hartley guinea pig with recurrent genital herpes. Twelve papers are clinical trials which includes one single group trial, one controlled trial, ten randomized controlled trials. All of the studies have shown that herbal medicine is effective in improving the symptom of genital herpes and decreasing the recurrent rate of genital herpes and also has immunoregulatory effect. Conclusions: This study shows that herbal medicine could be a good treatment option for genital herpes. However, more well-designed clinical studies and laboratory studies will be needed.