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PCR-T- RFLP Analyses of Bacterial Communities in Activatced Sludges in the Aeration Tanks of Domestic and Industrial Wastewater Treatment Plants

  • RHO SANG CHUL;AN NAN HEE;AHN DAE HEE;LEE KYU HO;LEE DONG HUN;JAHNG DEOK JIN
    • Journal of Microbiology and Biotechnology
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    • 제15권2호
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    • pp.287-295
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    • 2005
  • In order to compare bacteria] community structure and diversity in activated sludges, terminal restriction fragment length polymorphism (T-RFLP) of PCR-amplified 16s rDNAs was analyzed for 31 domestic and industrial wastewater treatment plants (WTPs). Regardless of the characteristics of the wastewaters, the bacteria] community structures of activated sludges appeared diverse and complex. In particular, activated sludges in domestic WTPs contained higher bacterial diversity than those in industrial WTPs. It was also found that terminal restriction fragment (T-RF) profiles derived from domestic WTPs were very similar with each other, although activated sludges were collected from different plants at different locations. Interestingly, activated sludges of a WTP where restaurant and toilet sewages of a company were managed showed a bacterial community structure similar to that of domestic WTPs. Activated sludges in leather industria] WTPs also showed a high similarity. However, other wastewaters possessed different bacterial communities, so that overall similarity was as low as about $30\%$. Since activated sludges from WTPs for domestic wastewaters and a company sewage appeared to hold similar bacterial communities, it was necessary to confirm if similar wastewaters induce a similar bacterial community. To answer this question, analysis of T-RFs for activated sludges, taken from another 12 domestic WTPs, was conducted by using a 6­FAM$^{TM}$-Iabeled primer and an automated DNA sequencer for higher sensitivity. Among 12 samples, it was again found that T-RF profiles of activated sludges from Yongin, Sungnam, Suwon, and Tancheon domestic WTPs in Kyonggi-do were very similar with each other. On the other hand, T-RF profiles of activated sludges from Shihwa and Ansan WTPs were quite different from each other. It was thought that this deviation was caused by wastewaters, since Ansan and Shihwa WTPs receive both domestic and industrial wastewaters. From these results, it was tentatively concluded that similar bacterial communities might be developed in activated sludges, if WTPs treat similar wastewaters.

A Study on the Relationship between Images of Colors Derived from Plants and Color Names

  • Masuda, Kinuko;Iwasaki, Yutaka
    • 한국조경학회:학술대회논문집
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    • 한국조경학회 2007년도 Journal of Landscape Architecture in Asia Vol.3
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    • pp.78-83
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    • 2007
  • The colors have a close to our life and there are many color names derived from plants in Japan. This study aimed to analyze relationship people and plants and investigated what the images of colors derived from plants related to the color names. Surveys on color images of abstract and concrete were conducted with adults who lived in the metropolitan area and in urban areas. The subjects were surveyed about images of similar colors derived from plants(3 type of similar two colors; pink, green and yellow) and asked to select a pair of color names(momo or pink, midori or green, yamabuki or lemon) corresponding to the color. The subjects were simultaneously presented with two similar colors and asked to give their perceptions of each color against 13 polar opposite pairs of terms or phrases using a Semantic Differential Scale, and asked to select the number of the 16 items concerned with life such as clothes and food. With the pink colors, many subjects did not feel opposite abstract images between these two similar colors and felt the same concrete images such as 'cosmetics' and 'clothes', and these color names such as momo and pink are confused. With the green colors and the yellow colors, many subjects felt opposite abstract images between these two similar colors such as 'natural - artificial' and 'rural - urban' and different concrete images such as 'plants' and 'plastics', and these two similar colors are distinguished by the color names such as 'midori - green' and 'yamabuki - lemon'. The results revealed that there are two patterns in the relationship colors derived from plants, color images and color names. In the pattern which images of colors derived from plants are connected with artificial things irrelevant to plants, the color names corresponding to the two similar colors are confused. On the other hand, in the pattern which images of colors derived from plants are connected with plants and artificial things, the two similar colors are distinguished by their color names. So demographics influenced the images of color, younger subjects compared with older tend to obviously distinguish images of colors derived from plants, and there are images of color influenced by the environment as a child. In both patterns, images of colors derived from plants are influenced by artificial things and natural colors are limited among many people. These results indicate that there are a few opportunities to see colors of plants which are full of variety. These results suggest that it is necessary to recognize colors derived from plants in relation to plants and to educate regarding the nature of plants, therefore landscape architecture focused on colors of plants is required.

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MULTIFRACTAL ANALYSIS OF A GENERAL CODING SPACE

  • Baek, In Soo
    • 충청수학회지
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    • 제19권4호
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    • pp.357-364
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    • 2006
  • We study Hausdorff and packing dimensions of subsets of a general coding space with a generalized ultra metric from a multifractal spectrum induced by a self-similar measure on a self-similar Cantor set using a function satisfying a H${\ddot{o}}$older condition.

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ON THE CONTINUITY AND GAUSSIAN CHAOS OF SELF-SIMILAR PROCESSES

  • Kim, Joo-Mok
    • 충청수학회지
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    • 제12권1호
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    • pp.133-146
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    • 1999
  • Let {X(t), $t{\geq}0$} be a stochastic integral process represented by stable random measure or multiple Ito-Wiener integrals. Under some conditions, we prove the continuity and self-similarity of these stochastic integral processes. As an application, we get Gaussian chaos which has some shift continuous function.

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NON-DIFFERENTIABLE POINTS OF A SELF-SIMILAR CANTOR FUNCTION

  • Baek, In-Soo;Kim, Young-Ha
    • East Asian mathematical journal
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    • 제19권2호
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    • pp.213-219
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    • 2003
  • We study the properties of non-diffenrentiable points of a self-similar Cantor function from which we conjecture a generalization of Darst's result that the Hausdorff dimension of the non-diffenrentiable points of the Cantor function is $(\frac{ln\;2}{ln\;3})^2$.

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화병(火病) 유사 병증의 한의학적 치료에 대한 임상연구 동향분석 - CNKI를 중심으로 (Analysis of Clinical Research Trends on Traditional Chinese Medicine Treatment for Depression Syndrome Similar to Hwabyung: Focusing on CNKI)

  • 최은지;서효원;김종우;정선용
    • 동의신경정신과학회지
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    • 제28권4호
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    • pp.349-358
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    • 2017
  • Objectives: To analyze whether a concept similar to Korean 'Hwabyung' exists in China. We investigated the status of clinical studies conducted in China for relevant diseases and trends of the traditional Chinese medicinal (TCM) treatment adopted in clinical studies. Methods: To explore the concept of a condition similar to Korean Hwabyung in China, we searched for the existence of concept and pattern that were parallel or similar to those of Hwabyung in TCM text-books and diagnostic guidelines. We searched and analyzed clinical studies of TCM treatment for depression syndrome similar to Hwabyung from CNKI using terms 'depression' and 'qi stagnation transforming into fire'. Using extracted data, characteristics of clinical research, herbal medicine, and acupuncture treatment used in the clinical research and their effects were systematically reviewed. Results: Symptoms of 'qi stagnation transforming into fire' were most similar to those of Hwabyung. Nine articles were selected from a total 258 articles. Most of them used DSM-IV or CCMD-3 for depression diagnosis. They applied 'diagnostic and efficacy guidelines for TCM diseases and syndromes' for pattern diagnosis of 'qi stagnation transforming into fire'. Danzhixiaoyao-san and Jiaweixiaoyao-san were found to be effective when they were used alone or in combination with antidepressants. Acupuncture treatment also showed remarkable effect on LR3, HT7, LI4, PC6, GV20 when it was used alone or in combination with antidepressants. However, careful interpretation is required because a small number of studies are included.Conclusions: 'Qi stagnation transforming into fire' seemed to have symptoms similar to Hwabyung. However, further research is needed to determine its diseases and pattern types compared to Hwabyung. According to included studies of 'depression with Qi stagnation transforming into fire', herbal medicine and acupuncture treatment tended to be effective in relieving depressive symptoms. However, more discussion is required for future application of herbal medicine and acupuncture for treating Hwabyung.

상관 계수를 이용한 유사 모집단 기반의 분광 반사율 추정 (Spectral Reflectance Estimation based on Similar Training Set using Correlation Coefficient)

  • 유지훈;하호건;김대철;하영호
    • 전자공학회논문지
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    • 제50권10호
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    • pp.142-149
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    • 2013
  • 일반적으로 영상의 색은 RGB 카메라 시스템의 red, green, blue 채널들을 사용하여 재현된다. 하지만 세 채널들의 정보만으로 실제 장면의 분광 반사율을 추정하는데 한계가 있다. 이 때문에 RGB 카메라 시스템은 색을 정확하게 재현하지 못한다. 이 한계를 극복하고 정확한 색을 재현하기 위해 다채널 카메라 시스템을 사용하여 분광 반사율을 추정하는 연구들이 활발히 진행되고 있다. 최근 분광 유사도를 사용하여 카메라 응답에 따라 기존 모집단에서 유사 모집단을 적응적으로 구성하는 분광 반사율 추정법이 소개되었다. 하지만 이 방법에는 평균 거리와 최대 거리 기반의 분광 유사도가 적용되었기 때문에 유사 모집단의 정확도가 저하된다. 본 논문에서는 유사 모집단의 정확도를 향상시키기 위해 상관 계수 기반의 분광 유사도가 적용된 분광 반사율 추정법을 제안하였다. 먼저 기존 모집단과 위너(Wiener) 추정법을 통해 획득된 분광 반사율 간의 상관 계수를 계산한다. 다음으로 상관 계수에 따라 기존 모집단에서 유사 모집단을 구성한다. 마지막으로 유사 모집단이 적용된 위너 추정법을 수행하여 분광 반사율을 추정한다. 제안된 방법과 이전의 방법들의 성능을 평가하기 위해 실험 결과를 비교하였다. 그 결과, 제안한 방법이 제일 우수한 성능을 나타내었다.

갯바위에서 분리한 미생물의 항균활성 분석 (Antibacterial Activity of Bacteria Isolated from Rocks on the Seashore)

  • 박인숙;오륜경;이민정;문지영;김영옥;남보혜;공희정;김우진;안철민;김동균
    • 한국수산과학회지
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    • 제48권6호
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    • pp.904-912
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    • 2015
  • There is a great deal of research interest regarding substitutes for antibiotics because of various obstacles to the efficacy and use of antibiotics. We isolated and analyzed diversity of microbiota which exhibited antibacterial activity against 23 pathogenic bacteria, to develop alternative agent of antibiotics. By investigating the microbiota from rocks on the seashore, we characterized and obtained various antibacterial material-producing bacteria. Thirty-one isolates belong to four genera and seven species, according to 16S rDNA sequence analysis, showed antibacterial activities against 23 pathogenic bacteria. The Identity of 16S rDNA sequences indicated three species of Bacillus, one species of Paenibacillus, one species of Pseudomonas and two species of Enterobacter. Two isolates were similar to Bacillus aerophilus, four isolates were similar to Bacillus pumilus, seven isolates were similar to Bacillus safensis, 15 isolates were similar to Paenibacillus polymyxa, respectively. In addition, one isolate was similar with Pseudomonas poae, one isolate was similar to Enterobacter asburiae, and one isolate was similar to Enterobacter ludwigii, respectively. Variations of antibacterial activity and level among the same species were indicated the diverse strains of isolates. Vibrio vulnificus showed the highest degree of growth inhibition by 29 isolates. Further studies regarding antibacterial materials and bacteria suggest that development of probiotic strains or alternative agents to antibiotics.

Radar Polygon 기법의 개발 : 유사강우발생 확률에 근거한 면적강우량 산정기법 (Development of Radar Polygon Method : Areal Rainfall Estimation Technique Based on the Probability of Similar Rainfall Occurrence)

  • 조운기;이동률;이재현;김동균
    • 한국수자원학회논문집
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    • 제48권11호
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    • pp.937-944
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    • 2015
  • 본 연구에서는 실측자료를 기반으로 한 새로운 면적강우량 산정기법인 '레이더 폴리곤 기법(Radar polygon Method, PRM)'을제시하였다. RPM은(1) 강우공간분포의 실측자료인 기상레이더 자료를 이용하여 지점관측소가 위치한 곳에서의 강우강도와 주변지역의 강우강도를 비교하여 유사강우 발생지도 작성; (2) 위의 단계를 관측소별로 반복하여 각 관측소별 유사강우 발생 확률 지도 작성; (3) 주어진 격자에서의 각 관측소의 유사강우 발생 확률의 비교를 통한 지배범위 결정의 알고리즘으로 관측소별 가중치를 결정하는 방법이다. RPM 방법을 안성천 유역에 적용하여 Thiessen법과 결과를 비교하였다. 안성천 유역의 경우 RPM과 Thiessen방법에 근거한 다각형의 공간적 형태는 관측소 위치의 강우 특성에 따라 차이를 보였으나 관측소별 가중치 값의 차이는 크지 않았다. 본 연구는 관측기간 및 정확도의 문제로 인하여 제한적으로 활용되어 온 레이더 강우관측자료의 새로운 활용분야를 개척하였다는 점에서 큰 의미를 찾을 수 있다.

이동 객체의 유사 부분궤적 검색을 위한 시그니쳐-기반 색인 기법 (Signature-based Indexing Scheme for Similar Sub-Trajectory Retrieval of Moving Objects)

  • 심춘보;장재우
    • 정보처리학회논문지D
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    • 제11D권2호
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    • pp.247-258
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    • 2004
  • 최근 비디오 데이타베이스, 시공간 데이타베이스, 모바일 데이타베이스와 같은 데이타베이스 응용 분야에서 이동 객체를 기반으로 하는 검색 기법에 관한 연구가 활발히 이루어지고 있다. 본 논문에서는 이동 객체의 궤적에 대한 효율적인 유사 부분궤적 검색을 지원하는 새로운 시그니쳐-기반 색인 기법을 제안한다. 제안하는 시그니쳐-기반 색인 기법은 궤적 데이타를 토대로 궤적 시그니쳐를 생성하는 방법에 따라 중첩 시그니쳐-기반 색인 기법(Superimposed signature-based Indexing scheme for similar Sub-trajectory Retrieval : SISR)과 합성 시그니쳐-기반색인 기법(Concatenated signature-based Indexing scheme for similar Sub-trajectory Retrieval : CISR)으로 나뉜다. 생성된 궤적 시그니쳐 정보는 시그니쳐 파일에 저장되고, 검색시 주어진 사용자 질의 궤적 정보를 기반으로 데이타 파일을 직접 접근하기 전에 전체 궤적 시그니쳐들을 탐색하여 필터링을 수행한다. 이를 통해 데이타 파일의 검색 범위를 현저히 줄임으로써 검색 성능을 향상시킨다. 또한 검색된 궤적 데이터와의 유사성을 측정하기 위해 k-워핑 알고리즘을 적용시켜 검색의 효율성을 높인다. 마지막으로, 순차 색인 기법, SISR기법, 그리고 CISR 기법을 삽입시간, 검색 시간 그리고 부가 저장 공간측면에서 성능 평가를 수행한다. 성능 평가 결과, 제안하는 두 가지 기법이 검색 성능 측면에서 순차 색인 기법에 비해 성능이 우수함을 나타내고, 아울러 SISR 기법이 CISR 기법에 비해 보다 우수한 성능을 보인다.