• Title/Summary/Keyword: Signal amplification

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Interference Neutralization for Small-Cell Wireless Networks (스몰셀 무선망 간섭 상쇄 기법 연구)

  • Jeon, Sang-Woon;Jung, Bang Chul
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.38A no.12
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    • pp.1117-1124
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    • 2013
  • As the recently soaring wireless traffic, small-cell techniques have been actively studied in order to support such a wireless demand for cellular wireless networks. This paper focuses on the inter-cell interference neutralization to resolve the main barrier for implementing small-cell cellular networks. Assuming that each message is delivered to the final destination by the help of base stations or relays, ergodic interference neutralization is proposed, which exploits the time-varying nature of wireless channels. The previous approach based on amplify-and-forward (AF) suffers from severe performance degradation in the low signal-to-noise (SNR) regime due to noise amplification. On the other hand, the proposed interference neutralization based on recently developed compute-and-forward (CF) fixes such a problem and improves the performance in the low SNR regime.

Cloning, Characterization, and Expression of Xylanase A Gene from Paenibacillus sp. DG-22 in Escherichia coli

  • Lee, Tae-Hyeong;Lim, Pyung-Ok;Lee, Yong-Eok
    • Journal of Microbiology and Biotechnology
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    • v.17 no.1
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    • pp.29-36
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    • 2007
  • The xynA gene encoding the xylanase A of Paenibacillus sp. DG-22 was isolated with a DNA probe obtained by PCR amplification, using degenerated primers deduced from the amino acid residues of the known N-terminal region of the purified enzyme and the conserved region in the family 11 xylanases. The positive clones were screened on the LB agar plates supplemented with xylan, by the Congo-red staining method. The xynA gene consists of a 630-bp open reading frame encoding a protein of 210 amino acids, and the XynA preprotein contains a 28-residues signal peptide whose cleavage yields a l82-residues mature protein of a calculated molecular weight of 20,000Da and pI value of 8.77. The cloned DNA fragment also has another ORF of 873 nucleotides that showed 76% identity to the putative transcriptional activator of Bacillus halodurans C-125. Most of the xylanase activity was found in the periplasmic space of E. coli. The xynA gene was subcloned into pQE60 expression vector to fuse with six histidine-tag. The recombinant xylanase A was purified by heating and immobilized metal affinity chromatography. The optimum pH and temperature of the purified enzyme were 6.0 and $60^{\circ}C$, respectively. This histidine-tagged xylanase A was less thermostable than the native enzyme.

Quantification of Genetically Modified Canola GT73 Using TaqMan Real-Time PCR

  • Kim, Jae-Hwan;Song, Hee-Sung;Kim, Dong-Hern;Kim, Hae-Yeong
    • Journal of Microbiology and Biotechnology
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    • v.16 no.11
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    • pp.1778-1783
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    • 2006
  • Event-specific PCR detection methods are the primary trend in genetically modified (GM) plant detection owing to their high specificity based on the flanking sequence of the exogenous integrant. Therefore, this study describes a real-time PCR system for event-specific GM canola GT73, consisting of a set of primers, TaqMan probe, and single target standard plasmid. For the specific detection of GT73 canola, the 3'-integration junction sequence between the host plant DNA and the integrated specific border was targeted. To validate the proposed method, test samples of 0, 1, 3, 5, and 10% GT73 canola were quantified. The method was also assayed with 15 different plants, and no amplification signal was observed in a real-time PCR assay with any of the species tested, other than GT73 canola.

Biochemical Properties and Substrate Specificity of Lipase from Staphylococcus aureus B56

  • Jung, Woo-Hyuk;Kim, Hyung-Kwoun;Lee, Chan-Yong;Oh, Tae-Kwang
    • Journal of Microbiology and Biotechnology
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    • v.12 no.1
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    • pp.25-30
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    • 2002
  • A lipase of Staphylococcus aureus B56 was purified from a culture supernatant and its molecular weight was estimated to be 45 kDa by SDS-PAGE. The optimum temperature and pH for the hydrolysis of olive oil was $42^{\circ}C$ and pH 8-8.5, respectively. The enzyme was stable up to $55^{\circ}C$ in the presence of $Ca^++$ at pHs 5-11. The lipase gene was cloned using the PCR amplification method. The sequence analysis showed an open reading frame of 2,076 bp, which encoded a preproenzyme of 691 amino acids. The preproenzyme was composed of a signal sequence (37 aa), propeptide (255 aa), and mature enzyme (399 aa). Based on a sequence comparison, lipase B56 constituted of a separate subgroup among the staphylococcal lipase groups, such as S. aureus PS54 and S. haemolyticus L62 lipases, and was distinct from other lipases in their optimum pH and substrate specificity.

Performance Analysis of Adaptive Collaborative Communications in Wireless Networks (무선네트워크에서 적응형 협력통신의 성능 분석에 관한 연구)

  • Khuong Ho Van;Kong Hyung-Yun;Jeong Hwi-Jae
    • The KIPS Transactions:PartC
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    • v.13C no.6 s.109
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    • pp.749-756
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    • 2006
  • Broadcast nature of wireless medium and path-loss reduction create a favourable condition for collaborative communications (CC) among single-antenna users to gain the powerful benefits of multi-antenna system without the demand for physical arrays. This paper proposes a CC strategy adapting to the propagation environment changes by optimizing the transmit signal amplification factors to simplify the structure of maximum likelihood (ML) detector and to obtain the minimum error probability as well. The closed-form BER expression was also derived and compared to the simulation results to evaluate the performance of the suggested solution. A variety of numerical results revealed the cooperation significantly outperforms non-cooperative counterpart under flat Rayleigh fading channel plus AWGN (Additive White Gaussian Noise).

Digital Logic Extraction from Quantum-dot Cellular Automata Designs (Quantum-dot Cellular Automata 회로로부터 디지털 논리 추출)

  • Oh, Youn-Bo;Lee, Eun-Choul;Kim, Kyo-Sun
    • Proceedings of the KIEE Conference
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    • 2006.10c
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    • pp.139-141
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    • 2006
  • Quantum-dot Cellular Automata (QCA) is one of the most promising next generation nano-electronic devices which will inherit the throne of CMOS which is the domineering implementation technology of large scale low power digital systems. In late 1990s, the basic operations of the QCA cell were already demonstrated on a hardware implementation. Also, design tools and simulators were developed. Nevertheless, its design technology is not quite ready for ultra large scale designs. This paper proposes a new approach which enables the QCA designs to inherit the verification methodologies and tools of CMOS designs, as well. First, a set of disciplinary rules strictly restrict the cell arrangement not to deviate from the predefined structures but to guarantee the deterministic digital behaviors. After the gate and interconnect structures of the QCA design are identified, the signal integrity requirements including the input path balancing of majority gates, and the prevention of the noise amplification are checked. And then the digital logic is extracted and stored in the OpenAccess common engineering database which provides a connection to a large pool of CMOS design verification tools. Towards validating the proposed approach, we designed a 2-bit QCA adder. The digital logic is extracted, translated into the Verilog net list, and then simulated using a commercial software.

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통신위성 전력제어 및 분배장치 설계 및 해석

  • Choi, Jae-Dong
    • Aerospace Engineering and Technology
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    • v.2 no.1
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    • pp.108-116
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    • 2003
  • This research presents the design and analysis of PCDU(Power Control & Distribution Unit) of communication satellite. The PCDU of a spacecraft must provide adequate power to each subsystem and payload during mission life, and it also needs high reliability and performance in space environment. A control circuit of the PCDU include bus sensing and filter circuits, error signal amplification circuit, error compensation circuit of SAS(Shunt Assembly Switch) and BPC(Battery Power Converter). The phase margin and DC gain for the designed circuits are analyzed through the frequency response characteristics of the compensated control circuit. And also the transfer function of the battery power converter circuit are discussed at the battery CCCM(Charge Continuous Conduction Mode) and battery C/DCCM(Continuous/Discontinuous Conduction Mode).

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A K-Band Low-Power Miniaturized Hyperthermia System

  • Kim, Dong-Ki;Kim, Ki-Hyun;Oh, Jung-Min;Park, Young-Rak;Kwon, Young-Woo
    • Journal of electromagnetic engineering and science
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    • v.9 no.4
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    • pp.188-193
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    • 2009
  • A K-band low-power miniaturized planar-type hyperthermia system was developed to replace massive and expensive equipment. The system consists of a VCO with a buffer amplifier, a high-power amplifier module, a 20-dB-coupled line coupler, a chip circulator and two power detectors for signal generation, amplification and power monitoring. All these components have been implemented in planar form on two module blocks. The total size of the hyperthermia system was less than $10\times6.5\times3\;cm^3$. In order to verify the system performance, ablations were carried out on nude mice xenografted with human breast cancer. Ablation results show performance comparable to the massive components-based system. This work shows the feasibility of a low-cost miniaturized hyperthermia system for practical clinical applications.

A Simplified GaAs MESFET Modeling for the Design of Ultrabroad-Band Microwave Amplifiers (초광대역 마이크로파 증폭기 설계를 위한 단순화한 GaAs MESFET 모델링)

  • Yoon, Young-Chul;Kim, Byung-Chul;Ahn, Dal;Chang, Ik-Soo
    • Journal of the Korean Institute of Telematics and Electronics
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    • v.26 no.9
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    • pp.1308-1316
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    • 1989
  • A simplified 10-element GaAs MESFET equivalent circuit model has been presented which is suitable for the design of ultrabroad-band microwave small-signal amplification, the these circuit element values are extracted from measured S-parameters using complex-curve fitting algorithm. Packaged GaAs MESFET equivalent circuits are composed of intrinsic \ulcornermodel and several extrinsic elements at microwave frequencies, of which the largest effects are caused by package lead inductances. If these are eliminated from measured S-parameters, newly obtained S-parameters are closed to intrinsic \ulcornermodel, and the rest element values can be easily extracted. The modeling results applied to the packaged GaAs MESFET NE71083 are almost equal between the measure S-parameters and the mideled S-parameters within b 2% errors from DC to 8GHz, and errors are increased to \ulcorner% upto 12GHz wide bandwidth.

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Production and Secretion of Human Interleukin-18 in Transgenic Tobacco Cell Suspension Culture

  • Sharma, Niti;Kim, Tae-Geum;Yang, Moon-Sik
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.11 no.2
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    • pp.154-159
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    • 2006
  • Interleukin-18 (IL-18), otherwise known as interferon-gamma-inducing factor (IGIF), is one of several well characterized and important cytokines that contribute to host defenses. The complementary DNA (cDNA) of mature human interleukin-18 gene (hIL-18) was fused with the signal peptide of the rice amylase 1A gene (Ramy1A) and introduced into the plant expression vector under the control of a duplicated CaMV 35S promoter. The recombinant plasmid was transformed into tobacco (Nicotiana tabacum L. cv Havana) using the Agrobacterium-mediated transformation method. The integration of the hlL-18 gene into the genome of transgenic tobacco plants was confirmed by polymerase chain reaction (PCR) amplification and its expression was observed in the suspension cells that were derived from the transgenic plant callus by using Northern blot analysis. The hlL-18 protein was detected in the extracts of the transgenic callus and in the medium of the transgenic tobacco suspension culture by using immunoblot analysis. Based upon enzyme-linked immunosorbant assay (ELISA) results, the expression level of the hlL-18 protein approximated $166{\mu}g/L$ in the suspension culture medium. Bioassay results from the induction of $interferon-{\gamma}$ from a KG-1 cell line indicated that the hlL-18 secreted into the suspension culture medium was bioactive.