• Title/Summary/Keyword: Shuttle

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Design of a disk cam with remote follower in a tappet shedding device (타펫개구장치에서의 원격종동절 캠 설계)

  • 신재균;이종기;안홍태
    • Journal of the Korean Society for Precision Engineering
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    • v.21 no.1
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    • pp.167-172
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    • 2004
  • A method for the design of a disk cam with a remote follower is presented. ADAMS software is utilized for the design process. The whole process is applied for the design of a cam profile to be used in a tappet shedding device of a shuttle loom. First the desired motion of the remote follower, the hearld frame in this case, is determined to improve the characteristic of a certain weaving process. Next, the desired displacement data is input to the ADAMS software and the cam profile is obtained as an output. To test the validity of the proposed method, designed cams were actually manufactured and implemented on a experimental loom. And with the help of potentiometer type displacement transducers, the motion of the remote followers were actually recorded. And the result was sufficient to prove the validity of the presented method.

Expression of a Bacillus subtilis Mannanase Gene in Corynebacterium lactofementum (Corynebacterium lactofermentum에서 Bacillus subtilis의 Mannanase 유전자 발현)

  • Yoon, Ki-Hong
    • Microbiology and Biotechnology Letters
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    • v.37 no.4
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    • pp.405-407
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    • 2009
  • A Bacillus subtilis mannanase gene was subcloned into an Escherichia coli- Corynebacterium lactofermentum shuttle vector pHE83, and the resultant plasmid pHE83M was transferred into an endogenous plasmid-free strain of C. lactofermentum. Mannanase produced by the recombinant C. lactofermentum (pHE83M) was secreted extracellulary at the level of 86%, and the remaining activity of mannanase was detected in the cell-free extract. The maximum mannanase productivity of the recombinant strain reached 8.1 unit/mL in the culture filtrate of LB medium. According to the zymogram of mannanase on SDS-PAGE, it was found that the mannanase produced by the recombinant C. lactofermentum could be maintained stably with a migration identical to the mannanase produced by the parental strain, B. subtilis WL-3.

Theoretical Interpretation of the UV Obervations of Elliptical Galaxies from NASA Space Mission

  • Lee, Young-Wook-
    • Bulletin of the Korean Space Science Society
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    • 1993.04a
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    • pp.15-15
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    • 1993
  • During the past two decades, ultraviolet(UV) observations of elliptical galaxies and spiral bulges from the OAO-2 and HE satellites have established that the upturn in the specturm below 2000A is a common feature of these early-type system Because of its important implications for the formation of galaxies, astuonemical literature is replete with nurmerous discussions about the origin of the UV flux yet the situation remains controversal. New light on the problem is shed by the recent observations Some the Astro-1 space shuttle mission which suggest that the metal-poor horizontal-branch(HB) stars merit more detailed consideration for the origin of the UV flux It is shown here that such stars in these systems can indeedexplain the UV flux It is suggested that ttle obserred correlation between the UVupturn and total mass is due to the Possibility that the more massive galaxies tend to form earlier than the less massive galaxies as a result of mere efficient star formation in denser environments. It remains a task of mere detailed population synthesis nudels, now in progress, to put these results on a firmer quantitative basis.

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Study of synchrotron photons by the Prototype Synchrotron Radiation Detector in the space

  • Park, Wung-Hoa;Lee, Man-Woo;Kim, Kyung-Suk;Kim, Guin-Yun;Yang, Jong-Man;Son, Dong-Chul
    • Bulletin of the Korean Space Science Society
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    • 2008.10a
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    • pp.23.1-23.1
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    • 2008
  • A space shuttle with a Prototype Synchrotron Radiation Detector (PSRD) was launched in 2001. PSRD was set in the Endeavour payloads and got data for 12 days. The purpose of PSRD is to measure synchrotron photons which are created by high energy charged particles near earth. Synchrotron photons are confused with background photons. We studied how to separate synchrotron photons from backgrounds.

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pKT230 벡터를 이용한 Pseudomonas sp. P20의 2,3-Dihydroxybiphenyl Dioxygenase 유전자의 클로닝

  • Kim, Ji-Young;Kim, Chi-Kyung;Ka, Jong-Ok;Min, Kyung-Hee;Park, Yong-Keun
    • Microbiology and Biotechnology Letters
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    • v.24 no.6
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    • pp.657-663
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    • 1996
  • Pseudomonas sp. P20 isolated from the polluted environment is capable of degrading biphenyl and 4-chlorobiphenyl. The pcbABCD genes responsible for degradation of biphenyl and 4-chlorobiphenyl were cloned using pBluescript SK(+) from the chromosomal DNA of Pseudomonas sp. P20 to construct pCK1 and pCK102, harbouring pcbABCD and pcbCD, respectively. The 2, 3-DHBP dioxygenase gene, pcbC, was cloned again from pCK102 by using pKT230 which is known as a shuttle vector and pKK1 hybrid plasmid was constructed. The E. coli KK1 transformant obtained by transforming the pKK1 into E. coli XL1-Blue showed 2, 3-DHBP dioxygenase activity. The specific 2, 3-DHBP dioxygenase activity of E. coli KK1 was similar to that of the E. coli CK102, but much higher than those of the natural isolates, Pseudomonas sp. DJ-12 and Pseudomonas sp. P20.

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A Second-Order Analysis of VM Heat Pumps (VM열펌프의 2차해석)

  • Choi, Y.S.;Jeong, E.S.
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.8 no.2
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    • pp.208-218
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    • 1996
  • Performance of a VM heat pump is considerably affected by various losses, such as enthalpy dump, reheat loss, pumping loss, conduction loss and shuttle loss. A second-order analysis model of VM heat pumps, which allows consideration of the major losses, was presented. Actual heat transfer rates for heat exchangers were calculated from the heat transfer rates obtained by the adiabatic analysis and various losses. New effective temperatures of heat exchangers were calculated from the actual heat transfer rates and the mean heat transfer coefficients until there was no appreciable change in the effective temperatures. Effects of design parameters, such as phase angle, swept volume ratio, regenerator length and speed on heating capacity, cooling capacity and COP were shown.

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Common Cause Failure Problems in Ultra-High Reliability Systems-A View Point on Common Cause Internal Effects and Statistical Principles (초신뢰성 시스팀에서의 공통원인 실패문제-공통원인의 내부적 효과 및 통계학적 원리의 관점에서)

  • Park, P.;Ko, K.H.;Kim, C.S.;Kim, H.K.;Oh, H.S.
    • Electronics and Telecommunications Trends
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    • v.8 no.3
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    • pp.39-52
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    • 1993
  • This study involves a Common Cause Failure (CCF) problem on the ultra-high reliability required system development such as war game operations, nuclear power control, air traffic control, space shuttle missions, and large scale network communication system. The system situation problems are defined according to CCF, reliability and system fault identifications for the development cast verifications in the multi-version redundant software system. Then, CCF analysis of redundant system, system principles and statistical dependence are also described. This validation oh the CCF in the human software interaction system will notify software engineers to conceive what really is CCF contribution factor, not only the internal but the external ones.

Isolation and sequence analysis of a small cryptic plasmid from Lactobacillus farciminis KCTC3681 (Lactobacillus farciminis로부터 미지의 작은 플라스미드의 분리와 염기서열 분석)

  • Lee, Eun-Mo;Choi, Shin-Geon
    • Journal of Industrial Technology
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    • v.28 no.B
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    • pp.53-57
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    • 2008
  • From the extensive screening for small cryptic plasmid among about 23 lactic acid bacteria (LAB), 2.4 kb of cryptic plasmid was isolated from Lactobacillus farciminis strain KCTC 3681 and named as pLF24. The plasmid pLF24 was a circular molecule of 2,396 base-pairs in length with a G+C content of 38%. Two protein-coding sequences could be predicted. ORF1 and ORF2 showed homologies to plasmids of gram-positive bacteria. The replication protein coded by ORF2 and the plus origin, were similar to replication regions of other gram-positive bacteria as shown in plasmids such as pLH2, pLS141-1 and pLC2. The nucleotide sequence of pLF24 was deposited into Genbank data base with an accession number of EU429343. The newly isolated plasmid can be used for construction of shuttle vector in Lactobacillus bacteria.

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Expression of heterologous genes using the slpA promoter and signal sequence in Lactobacilli

  • Gang, Seung-Ha;Bok, Jin-Deok;Jo, Gwang-Geun;Jo, Jae-Sun;Choe, Yun-Jae
    • 한국생물공학회:학술대회논문집
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    • 2000.11a
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    • pp.202-205
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    • 2000
  • A gene coding $endo-{\beta}$,-1, 4 glucanase from Actinomyces sp. KNG40 and phytase from Hansenula polymorpha were cloned into Esherichia coli JM101 by using E. coli/Lactobacillus shuttle vector pNZ3004 and pNZ123. The plasmid p3PS(1-4) and p123(1-4) have slpA promoter and slpA signal sequence. So, I constructed expression vectors, p3PS(1-4)Endo, phy and p123(1-4)Endo, phy. These constructed vector was transformed in target host Lactobacillus gasseri and reutri. These transformed host expressed endoglucanase and phytase as extracellular fraction. In the enzyme activity of the same vector, host L, gasseri was higher activity than L. reuteri. This indicates that L. gasseri recongnize promoter and signal sequence very well.

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Development of a Vent Analysis Method for Multiple Compartments Connected Through Multiple Ports (다중 Port로 연결된 다중 격실 Vent 해석 기법 개발)

  • Ok, Ho-Nam;Kim, In-Sun
    • 유체기계공업학회:학술대회논문집
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    • 2006.08a
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    • pp.547-550
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    • 2006
  • An analysis method is developed for the prediction of venting in multiple compartments which are connected in series or parallel through multiple ports. The existing method by the authors is modified to remove the limitation in number of ports and compartments, and the more general polytropic relation or solution of the additional energy equation replaces the previous isentropic relation allowing the prediction of pressure rise in addition to pressure drop. The accuracy of the method is verified by comparison with the results by NASA Flap code for the problem of pressure drop in a payload in the Space Shuttle cargo bay. It is expected that this method will be a useful tool in prediction of the pressure variation in a payload or payload capsule without mentioning the payload fairing itself.

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