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Expression of a Bacillus subtilis Mannanase Gene in Corynebacterium lactofementum  

Yoon, Ki-Hong (Department of Food Science & Biotechnology, Woosong University)
Publication Information
Microbiology and Biotechnology Letters / v.37, no.4, 2009 , pp. 405-407 More about this Journal
Abstract
A Bacillus subtilis mannanase gene was subcloned into an Escherichia coli- Corynebacterium lactofermentum shuttle vector pHE83, and the resultant plasmid pHE83M was transferred into an endogenous plasmid-free strain of C. lactofermentum. Mannanase produced by the recombinant C. lactofermentum (pHE83M) was secreted extracellulary at the level of 86%, and the remaining activity of mannanase was detected in the cell-free extract. The maximum mannanase productivity of the recombinant strain reached 8.1 unit/mL in the culture filtrate of LB medium. According to the zymogram of mannanase on SDS-PAGE, it was found that the mannanase produced by the recombinant C. lactofermentum could be maintained stably with a migration identical to the mannanase produced by the parental strain, B. subtilis WL-3.
Keywords
Bacillus subtilis WL-3; Corynebacterium lactofermentum; expression; mannanase;
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