Expression of heterologous genes using the slpA promoter and signal sequence in Lactobacilli

  • 강승하 (서울대학교 농생명공학부, 중앙 바이오텍(주)) ;
  • 복진덕 (서울대학교 농생명공학부, 중앙 바이오텍(주)) ;
  • 조광근 (서울대학교 농생명공학부, 중앙 바이오텍(주)) ;
  • 조재순 (서울대학교 농생명공학부, 중앙 바이오텍(주)) ;
  • 최윤재 (서울대학교 농생명공학부, 중앙 바이오텍(주))
  • Published : 2000.11.09

Abstract

A gene coding $endo-{\beta}$,-1, 4 glucanase from Actinomyces sp. KNG40 and phytase from Hansenula polymorpha were cloned into Esherichia coli JM101 by using E. coli/Lactobacillus shuttle vector pNZ3004 and pNZ123. The plasmid p3PS(1-4) and p123(1-4) have slpA promoter and slpA signal sequence. So, I constructed expression vectors, p3PS(1-4)Endo, phy and p123(1-4)Endo, phy. These constructed vector was transformed in target host Lactobacillus gasseri and reutri. These transformed host expressed endoglucanase and phytase as extracellular fraction. In the enzyme activity of the same vector, host L, gasseri was higher activity than L. reuteri. This indicates that L. gasseri recongnize promoter and signal sequence very well.

Keywords