• 한국양식학회지
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• 제6권3호
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• pp.159-171
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• 1993

대하의 2oea와 mysis 기의 성장과 생존에 대한 밀도, 빛, 수온, 먹이의 영향은 다음의 조사결과와 같다. 사육 밀도에 따른 유생의 생존율은 500마리/l에서 보다 200, 300마리/l일때 휠씬 높았고, 사육밀도 100, 200, 300, 500마리/l일때 생존율은 90.3, 95.2, 94.3, $86.7\%$ 이었다. 규조를 혼합하여 먹이로 공급한 광주기 실험에서는 24시간 연속 조명하였을 때 생존율이 $96.5\%$로 자연광을 이용할 때 $(79.0\%)$와 24시간 암흑 처리할 때 $(4.3\%)$에 비해 매우 높은 생존과 빠른 성장을 보였다. 사육 수온은 $19^{\circ}C$에서 $97.3\%$의 생존을 보였지만 $22^{\circ}C$에 비해 성장이 늦었으며, $22^{\circ}C$에서 $96.7\%$ 의 생존율과 빠른 성장을 보였다. 수온 $25^{\circ}C$에서는 $51.6\%$의 생존율로 $19,\;22^{circ}C$에 비해 생존율과 성장이 저조하였다. 먹이생물로는 6종의 규조(A. normaii, C. nomaii, C. simplex, N. clostuium, P tricomutum, S. costatum, T weissflogii)를 혼합하여 공급하였을 때, 연속 조명으로 사육할 경우, $97.3\%$의 가장 높은 생존율과 최고의 성장을 나타내었으며, 1종의 규조만을 먹이생물로 공급하였을 때에는 S. costatum을 공급하였을 때의 생존율 $82.1\%$, T. weissflogii의 $80.6\%$ 를 제외하고는 모두 $62\%$이하의 생존율과 저조한 성장을 나타내었다.

• 한국식생활문화학회지
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• 제9권1호
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• pp.71-77
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• 1994

제조 조건을 달리한 4가지 chitin으로부터 제조된 chitosan의 물리화학적 특성인 점도와 탈아세틸화도를 측정하였으며, 4종류의 chitosan이 첨가된 깍두기를 제조하여 chitosan을 첨가하지 않은 깍두기를 대조군으로 하여 $20^{\circ}C$에서 2일, 4일, 6일, 8일간 저장하면서 저장기간에 따른 pH, 적정산도, 깍두기 국물의 점도 및 미생물 수의 변화를 측정한 결과는 다음과 같다. Chitosan 용액의 정도는 $313.1{\sim}98.8\;cps$로 나타났으며, chitin 추출시 염산의 농도가 낮은 것이 점도가 높았으며, 반응 온도가 낮을 때 더 높은 점도를 나타냈다. 그리고 chitosan 용액의 유동학적 특성은 의가소성 유체(pseudoplastic fluid)의 특성을 나타내었다. IR 분석 결과 chitosan의 탈아세틸화도는 $92{\sim}96%$로 높게 나타나, 점도와는 달리 chitosan의 원료가 되는 chitin의 제조조건에 의해서는 크게 영향받지 않았다. 모든 시료군에서 저장기간이 길어짐에 따라 pH는 감소하고 적정산도는 증가하는 경향을 나타냈으며, 대조군에 비해 chitosan 첨가군이 pH는 높고 적정산도는 낮은 값을 계속 유지하였다. 깍두기 국물의 점도는 저장기간이 길어짐에 따라 감소하는 경향을 나타냈고, 저장 8일째에는 대조군이 chitosan 첨가군에 비해 점도가 높게 나타났다. 총균수는 저장기간 전반에 걸쳐 chitosan 첨가군의 경우가 대조군에 비해 약간 많았고, Leuconostoc 속 미생물 수는 약간 적은 경향을 나타냈다. 또한 김치의 부패균으로 알려진 Lac. plantarum의 수에 있어서는 제조시 HCl의 농도가 낮고, 반응 온도가 높은 chitosan C를 첨가한 군이 대조군이나 다른 chitosan 첨가군에 비해 저장시 증가 속도가 약간 느렸다.

• 한국식품조리과학회지
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• 제30권2호
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• pp.153-160
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• 2014

The purpose of this study was to develop a rice education program and rice cooking practice for university students majoring in foodservice and nutrition. To achieve this purpose, 2 rounds of delphi survey was used by convenience sampling including total of 27 experts in the food and nutrition industry. Below are the findings. First, both the importance and suitability for macro-theme of theory education were in the following order: 'nutrition and health of rice', 'characteristics of rice in food science', 'history and culture of rice' and 'the consumption of rice. Second, the history and culture of rice(traditional culture related to rice, and the origin & history of rice), nutrition and health of rice(main nutritive components and efficacy of rice and prevention of disease), characteristics of rice as food(processed-products of rice and storage, the structure of rice), and the consumption of rice(current status of rice production and consumption) were found to be suitable for the micro-theme of theory education. Third, the education theme for rice cooking practice was determined. Barley sprout song-pyeon and beet song-pyeon were chosen for rice cake. For bread, coconut & walnut muffin, tofu & bean muffin, citron bread, red bean & sweet persimmon pound cake, and sweet potato bread were selected. Dong-rae-pa-jeon, Kimch-jeon-byeong, fried sweet potato fried shrimp were developed for the main dishes.

• 한국식생활문화학회지
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• 제18권4호
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• pp.379-395
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• 2003

Regional foods is influenced by weather, local and social environment etc. The purpose of this study was to understand that regional foods in the middle of Chosun Dynasty through Domundaejac. Some of representative dishes of the Hansung and Kyonggi-do Area is duck(rice cake) of seasonally celebrated days, hangua(korea confectionary) and rice wine. Typical foods in Chungchong-do Area is included persimmon, jujube, watermelon and wax gourd. Some of the well-known food in Kangwon-do Area is pear, bangpungchuk(gruel), sukebyung(rice cake), woongjijunggua(korea confectionary) and sanat-kimchi(picked mustard leaves and stem) that is seemed characteristics of mountainous section. Some of the famous dishes in Cholla-do Area is backsanja(korea confectionary), tea, chuksun kimchi(bamboo shoot pickle), citrus fruits and ear shell. Kyongsan-do Area ia famous persimmon, bamboo fruit, dasik(korea confectionary) and yakban(glutinous rice cake). Hwanghae-do Area is famed pear and choshi as assumed the eatly form of gochujang(thick soypaste mixed with red pepper). Representative dishes are deljjuk(blue berry), herring, sangat-kimchi(picked mustard leaves and sterm) in Hamgyong-do Area and daemandu(big dumpling) in Pyongan-do Area. The coast area is famous for using lots of sea food. Fresh seafood is consumed raw, and clams, fish and seaweed are liberally added to soups and other dishes. The East coast is catched salmon, flatfish, codfish, sandfish and mackerel The West coast is catched yellow corvenia, lobster, tiny shrimp and large-eyed herring. The South coast is catched sea mussel, codfish and laver.

• Biotechnology and Bioprocess Engineering:BBE
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• 제3권2호
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• pp.71-77
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• 1998

The strain of Serratia marcescens QM B1466 produces selectively large amount of chitinolytic enzymes (about 1mg/L medium). Enzymatic hydrolysis of chitin to N-acetyl-${\beta}$-D-glucosamine (NAG) was performed with a system consisting of two hydrolases (chitinase and chitobiase) produced by optimization of a microbial host consuming chitin particles. For the development of Large-scale biological process for the production of NAG from chitinaceous waste, the selection and optimization of a microbial host, particle size of crab/shrimp chitin sources and initial induction time using chitin as a sole carbon source on chitinase/chitobiase production and NAG production were examined. Crab-shell chitin(1.5%) treated by dilute acid and , ball-milled with a normal diameter less than 250m gave the highest chitinase activity over a 7 days culture. Crude chitinase/ chitobiase solution obtained in a 10 L fed-batch fermentation showed a maximum activities of 23.6 U/mL and 5.1 U/mL, respectively with a feeding time of 3 hrs, near pH 8.5 at 30$^{\circ}C$.

• 한국수산과학회지
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• 제19권4호
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• pp.356-362
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• 1986

This experiment was designed to evaluate effects of freezing and frozen storage on survival of sanitary indicative bacteria in seafoods. Culture of bacteria such as Escherichia coli type I, Citrobacter freundii type I, Klebsiella aerogenes type I and Streptococcus faecalis was inoculated into homogenates of pollack, shrimp, and sardine frozen in a contact plate freezer at $-40^{\circ}C$ and chest freezer at $-20^{\circ}C$, stored at $-20^{\circ}C$, and then survival of the inoculated bacteria was determined over a period of 95 days. Coliform group was highly sensitive to freezing and frozen storage showing survival of about $2\%$ after 95 days of frozen storage at $-20^{\circ}C$, whereas Streptococcus faecalis was relatively resistant with $20\%$ survival rate. The sanitary indicative bacteria count was rapidly decreased in the early stage of frozen storage revealing 90 to $95\%$ loss of coliform group and 40 to $70\%$ loss in case of Streptococcus faecalis after 10 days storage. In determining recovery rate, most probable number (MPN) method gave more reproducible recovery of the tested strain than did the selected agar plate method.

• Journal of Microbiology and Biotechnology
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• 제12권2호
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• pp.228-234
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• 2002

Bacillus subtilis CAU131 (KCCM 10257) isolated from a fermented shrimp product produces subtilein, tentatively named as a bacteriocin, which exhibited a bactericidal effect against closely related species such as Bacillus subtilis ATCC 6633, Bacillus cereus ATCC 11778, and several other strains of Bacillus sp. The purification of the subtilein was achieved by applying a mono-Q anion exchange chromatography on FPLC and $C_18$ reverse-phase chromatography on HPLC. After purification, specific activity of subtilein was increased about 3,000-fold compared with culture broth and its molecular mass was about 5,000 Da on SDS-PAGE. The antimicrobial activity of subtilein was well maintained at acidic and neutral pHs between 3 and 8. Subtilein was relatively heat stable, and its antimicrobial activity remained for 2 h at $80^{\circ}C$. However, the activity was reduced after heating at $100^{\circ}C$, and about $80\%$ of the activity was found after 1 h incubation at $100^{\circ}C$. The treatment of Bacillus subtilis ATCC 6633 with subtilein led to morphological changes in stationary-phase cells and most cells appeared to be lysed.

• Food Science and Biotechnology
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• 제17권4호
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• pp.857-860
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• 2008

S-Adenosyl-L-methionine (SAM) is a bioactive material used in the treatment of depression, osteoarthritis, and liver disease. To obtain lactic acid bacteria (LAB) producing high concentrations of SAM, LAB were isolated from commercial kimchi and from prepared kimchi products that contained shrimp jeotgal (fermented salty seafood) or sand lance jeotgal or that were fermented at 5 or $10^{\circ}C$, respectively, when pH was 4.2 to 4.8 and titratable acidity 0.6 to 0.9. Among the 179 LAB strains isolated from the fermented kimchi products, the genus Leuconostoc produced the highest intracellular level of SAM (1.58 mM) and Lactobacillus produced the second highest level (up to 1.47 mM) in the strain culture. This is the first study to quantify SAM in LAB isolated from fermented kimchi prepared by a general kimchi recipe. Ultimately, the selected strains (Leuconostoc mesentroides subsp. mesenteroides/dextranicum KSK417, L. mesentroides subsp. mesenteroides/dextranicum KJM401, and Lactobacillus bifermentans QMW327) could be useful as starters to manufacture fermented foods containing high levels of SAM.

• 한국식생활문화학회지
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• 제10권4호
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• pp.291-300
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• 1995

The purpose of this paper is to survey various recipes of the roasted beef with twenty three classical cookboods written before 1943. The roasted beefis found total 32 times in the literature which can be classified into seven groups such as the roasted rib, roasted foot, roasted tail, roasted heart, roasted gall, roasted kidney and roasted fresh meat. The most frequent one is the roasted rib appearing eight times and the next is the roasted sliced beef with seasoning appearing seven. This proves that the those recipes have been the most favorite ones to Korean people for a long time. The roasted rib has been found since the middle of the 17th century, but the process of roasting ribs again with seasoning after three successions of dipping shortly into cold water in the midst of roast wasz disappeared. The roasted sliced beef with seasoning originated since the late 18th century, and the roasted beef with salt since the early 19th century which has been inherited as the roasted raw upper part of roasted beef recipes have been continued until today in the similar manner. Generally the roasted meat with bones and the roasted internal organs started in 1766 earlier than the roasted fresh meat by a century. The main ingredients were rib, foot, tail, heart, gall, kidney, fresh meat and knee bone, and the seasonings were mixtures of scallion stalk, garlic, pepper, oil, soy sauce and sesame seed powder. And peculiarly salted shrimp, pear juice, ginger were added to seasonings and pine nut powder was used as decorating ingredient.

• 한국식생활문화학회지
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• 제21권2호
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• pp.187-192
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• 2006

This study is to investigate the recognition and preference of tofu food among general consumers and housewives in order to develope new tofu menu. The questionnaires are consisted of general questions, style of dining out, frequency of dining, health status, preference of tofu, reason for prefer tofu. A total of 262 questionnaires were analyzed for statistical analysis. The statistical analysis was completed using SAS program (Version 8.2) for descriptive analysis and ${\chi}^2\;-test$. Main results of this study were as follows: Most of the respondents prefer Korean food, 70% of the respondents are general consumers while 73.5% of the respondents are housewives. The frequency of dining out was 1-2 times per week. The two groups bought pre-cooked food one to two times per week. Fourity seven percent of the general consumers and 50% of housewives did not like the taste of tofu due to plain flavor. The respondents overall preferred many different ways to prepare tofu dishes. The results also indicated that tofu dishes are used as side-dishes. Thirty three percent of house wives had tofu with miso soup and pan-fried tofu, while 29.6% of the general consumers had soft tofu stew. 34% of the general consumers preferred stuffed tofu with shrimp, while 35.5% of the housewives liked it. 17% of the general consumers liked grilled tofu with crab meat sauce while only 14.5% of the housewives preferred the menu. Tofu teriyaki was preferred among 8.2% of the general consumers while 13.2% of the housewives liked tofu teriyaki.