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Characterization of Subtilein, a Bacteriocin from Bacillus subtilis CAU131 (KCCM 10257)  

Park, Sung-Yong (Department of Food Science and Technology, Chung-Ang University)
Yang, Yong-Jae (Department of Food Science and Technology, Chung-Ang University)
Kim, Young-Bae (Graduate School of Biotechnology, Korea University)
Hong, Jae-Hoon (Department of Food Science and Technology, Kon-Yang University)
Lee, Chan (Department of Food Science and Technology, Chung-Ang University)
Publication Information
Journal of Microbiology and Biotechnology / v.12, no.2, 2002 , pp. 228-234 More about this Journal
Abstract
Bacillus subtilis CAU131 (KCCM 10257) isolated from a fermented shrimp product produces subtilein, tentatively named as a bacteriocin, which exhibited a bactericidal effect against closely related species such as Bacillus subtilis ATCC 6633, Bacillus cereus ATCC 11778, and several other strains of Bacillus sp. The purification of the subtilein was achieved by applying a mono-Q anion exchange chromatography on FPLC and $C_18$ reverse-phase chromatography on HPLC. After purification, specific activity of subtilein was increased about 3,000-fold compared with culture broth and its molecular mass was about 5,000 Da on SDS-PAGE. The antimicrobial activity of subtilein was well maintained at acidic and neutral pHs between 3 and 8. Subtilein was relatively heat stable, and its antimicrobial activity remained for 2 h at $80^{\circ}C$. However, the activity was reduced after heating at $100^{\circ}C$, and about $80\%$ of the activity was found after 1 h incubation at $100^{\circ}C$. The treatment of Bacillus subtilis ATCC 6633 with subtilein led to morphological changes in stationary-phase cells and most cells appeared to be lysed.
Keywords
Bacteriocin; Bacillus subtilis; subtilein;
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1 Banerjee, S. and J. N. Hansen. 1988. Structure and expression of a gene encoding the precursor of subtilin, a small protein antibiotic. J. Biol. Chem. 263: 9508-9514
2 Hoerner, T., V. Ungermann, H. Zahner, H. P. Fiedler, R. Utz, R. Kellner, and G. Jung. 1990. Comparative studies on the fermentative production of lantibiotics by Staphylococci. Appl. Microbiol. Biotechnol. 32: 511-517
3 Holt, J. G., N. R. Krieg, P. H. A. Sneath, J. T. Staley, and S. T. Williams. 1994. Bergey’s Manual of Determinative Bacteriology, 9th ed. Williams and Wilkins. Baltimore, U.S.A
4 Hyronimus B., M. C. Lee, and M. C. Urdaci. 1998. Coagulin, a bacteriocin-like inhibitory substance produced by Bacillus coagulans I4. J. Appl. Microbiol. 85: 42-50
5 Klaenhammer, T. R. 1993. Genetics of bacteriocins produced by lactic acid bacteria. FEMS Microbiol. Rev. 12: 39-86   PUBMED
6 Nakano, M. M. and P. Zauber. 1988. Anaerobic growth of a strict aerobe. Annu. Rev. Microbiol. 52: 165-190
7 Paik H. D., S. S. Bae, S. H. Park, and J. G. Pan. 1997. Identification and partial characterization of tochicin, a bacteriocin produced by Bacillus thuringiensis subsp. tochigiensis. J. Indust. Microbiol. Biotechnol. 19: 294-298
8 Paik, H. D., N. K. Lee, K. H. Lee, Y. I. Hwang, and J. G. Pan. 2001. Identification and partial characterization of cerein BS229, a bacteriocin produced by Bacillus cereus. J. Microbiol. Biotechnol. 11: 195-200
9 Mah, J. H., K. S. Kim, J. H. Park, M. W. Byun, Y. B. Kim, and H. J. Hwang. 2001. Bacteriocin with a broad antimicrobial spectrum, produced by Bacillus sp. isolated from Kimchi. J. Microbiol. Biotechnol. 11: 577-584
10 Galvez, A., M. Maqueda, E. Valdivia, A. Quesada, and E. Montoya. 1986. Characterization and partial purification of a broad spectrum antibiotic AS-48 produced by Streptococcus faecalis. Can. J. Microbiol. 32: 765-771
11 Kim, S. I., J. Y. Chang, I. C. Kim, and K. Lee. 2001. Characterization of bacteriocin from Bacillus subtilis cx1. Kor. J. Appl. Microbiol. Biotechnol. 29: 50-55
12 Bhunia, A. K., M. C. Johnson, and B. Ray. 1987. Direct detection of an antimicrobial peptide of Pediococcus acidilactici in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. J. Indust. Microbiol. 2: 319-322
13 Tramer, J. and G. G. Fowler. 1964. Estimation of nisin in food. J. Sci. Food Agric. 15: 522-528
14 Larsen, A. G., F. K. Vogensen, and J. Josephsen. 1993. Antimicrobial activity of lactic acid bacteria isolated from sour dough: Purification and characterization of bavaricin A, a bacteriocin produced by Lactobacillus bavaricus MI401, J. Appl. Bacteriol. 75: 113-122
15 Hurst, A. 1981. Nisin. Adv. Appl. Microbiol. 27: 85-123   DOI
16 Von Tersch, M. A. and B. C. Carlton. 1983. Bacteriocin from Bacillus megaterium ATCC 19123: Comparative studies with megacin A-216. J. Bacteriol. 155: 866-871
17 Cintas L. M., J. M. Rodriguez, M. F. Fernandez, K. Sletten, I. F. Nes, P. E. Hernandez, and H. Holo. 1995. Isolation and characterization of pediocin L50, a new bacteriocin from Pediococcus acidilactici with a broad inhibitory spectrum. Appl. Environ. Microbiol. 61: 2643-2648
18 Piva, A. and D. R. Headon. 1994. Pediocin A, a bacteriocin produced by Pediococcus pentosaceus FBB61. Microbiology 140: 697-702
19 Kim, S. Y., Y. M. Lee, S. Y. Lee, Y. S. Lee, J. H. Kim, C. Ahn, B. C. Kang, and G. E. Ji. 2001. Synergistic effect of citric acid and pediocin K1, a bacteriocin produced by Pediococcus sp. K1, on inhibition of Listeria monocytogenes. J. Microbiol. Biotechnol. 11: 831-837
20 Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227: 680-685   DOI   PUBMED   ScienceOn
21 Konisky, J. 1982. Collicins and other bacteriocins with established modes of action. Annu. Rev. Microbiol. 36: 125- 144
22 Montville, T. J. and K. Winkowaski. 1977. Biologically based preservation systems and probiotic bacteria, pp. 557- 577. In M. P. Doyle, L. R. Beuchat, and T. J., Montville (eds.), Food Microbiology: Fundamentals and Frontiers, American Society for Microbiology; Washington, DC, U.S.A
23 Pucci, M. J., E. R. Vedamuthu, B. S. Kunka, and P. A. Vandenbergh. 1988. Inhibition of Listeria monocytogenes by using bacteriocin PA-1 produced by Pediococcus acidilactici PAC 1.0. Appl. Environ. Microbiol. 54: 2349-2353
24 Marco, J., V. Belkum, J. Kok, G. Venema, H. Holo, I. F. Nes, W. N. Konings, and T. Abee. 1991. The bacteriocin lactococcin A specifically increases permeability of lactococcal cytoplasmic membranes in a voltage-independent, protein-mediated manner. J. Bacteriol. 173: 7934-7941
25 Schuler, F., R. Benz, and H. G. Sahl. 1989. The peptide antibiotic subtilin acts by formation of voltage-dependent multi-state pores in bacterial and artificial membranes. Eur. J. Biochem. 182: 181-186
26 Koo, K. M., N. K. Lee, Y. I. Hwang, and H. D. Paik. 2000. Identification and partial characterization of lacticin SA72, a bacteriocin produced by Lactococcus lactis SA72 isolated from Joet-gal. J. Microbiol. Biotechnol. 10: 488-495
27 Crupper, S. S., A. J. Gies, and J. J. Iandolo. 1997. Purification of Staphylococcin BacR1, a broad-spectrum bacteriocin. Appl. Environ. Microbiol. 63: 4185-4190
28 Naclrio, G., E. Ricca, M. Sacco, and M. D. Felice. 1993. Antimicrobial activity of a newly identified bacteriocin of Bacillus cereus. Appl. Environ. Microbiol. 59: 4313-4315
29 Nielsen, J. W., J. S. Dickson, and J. D. Crouse. 1990. Use of bacteriocin produced by Pediococcus acidilactici to inhibit Listeria monocytogenes associated with fresh meat. Appl. Environ. Microbiol. 56: 2142-2145
30 Babasaki, K., T. Takyo, Y. Shimonishi, and K. Kurahashi. 1985. Subtilosin A, a new antibiotic peptide produced by Bacillus subtilis 168: Isolation, structural analysis and biogenesis. J. Biochem. 98: 583-603
31 Eckner, K. F. 1992. Bacteriocins and food application. Dairy Food Environ. Sani. 12: 204-209
32 Giulian, G. G., R. L. Moss, and M. Greaser. 1983. Improved methodology for analysis and quantitation of proteins on one-dimensional silver-stained slab gels. Anal. Biochem. 129: 277-287
33 Horn, N., M. I. Martinez, J. M. Martinez, P. E. Hernandez, M. J. Gasson, J. M. Rodriguez, and H. M. Dodd. 1998. Production of pediocin PA-1 by Lactococcus lactis using the lactococcin A secretory apparatus. Appl. Environ. Microbiol. 64: 818-823