• Title/Summary/Keyword: Short range

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Variation of Amylose Content Using dsRNAi Vector by Targeting 3'-UTR Region of GBSSI Gene in Rice (GBSSI 유전자 3'UTR 영역의 발현 억제 dsRNAi 벡터를 이용한 아밀로스함량 조절 벼 개발)

  • Park, Hyang-Mi;Choi, Man-Soo;Chun, Areum;Lee, Jeung-Heui;Kim, Myeong-Ki;Kim, Yeon-Gyu;Shin, Dong-Bum;Lee, Jang-Yong;Kim, Yul-Ho
    • Korean Journal of Breeding Science
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    • v.42 no.5
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    • pp.515-524
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    • 2010
  • The amylose content of starch is a major factor in the texture of cooked cereal grains. Therefore, down-regulation of amylose synthesis is one of the alternative method to improve eating quality of rice. We developed transgenic rice plants designed to suppress granule-bound starch synthase I(GBSSI) gene using RNA interference(RNAi) technology. Transgenic plants with RNAi vector containing the 3'-UTR region of GBSSI showed a lower amylose content in rice endosperm than that of wild-type. The range of amylose content was 5.9~9.0% in the transgenic plants, whereas that of wild-type was 17.7~18.0%. Transgenic rices showed the decrease of short chain and the increase of long chain by analyzing chain length distribution of amylopectin in the endosperm. In the SEM micrographs, we found that compound starch granules in whole grains of the wild-type rice were readily split during fracturing, while the starch granules in RNAi-transgenic lines showed small voluminous, non-angular rounded bodies.

3D Model Construction and Evaluation Using Drone in Terms of Time Efficiency (시간효율 관점에서 드론을 이용한 3차원 모형 구축과 평가)

  • Son, Seung-Woo;Kim, Dong-Woo;Yoon, Jeong-Ho;Jeon, Hyung-Jin;Kang, Young-Eun;Yu, Jae-Jin
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.11
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    • pp.497-505
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    • 2018
  • In a situation where the amount of bulky waste needs to be quantified, a three-dimensional model of the wastes can be constructed using drones. This study constructed a drone-based 3D model with a range of flight parameters and a GCPs survey, analyzed the relationship between the accuracy and time required, and derived a suitable drone application technique to estimate the amount of waste in a short time. Images of waste were photographed using the drone and auto-matching was performed to produce a model using 3D coordinates. The accuracy of the 3D model was evaluated by RMSE calculations. An analysis of the time required and the characteristics of the top 15 models with high accuracy showed that the time required for Model 1, which had the highest accuracy with an RMSE of 0.08, was 954.87 min. The RMSE of the 10th 3D model, which required the shortest time (98.27 min), was 0.15, which is not significantly different from that of the model with the highest accuracy. The most efficient flight parameters were a high overlapping ratio at a flight altitude of 150 m (60-70% overlap and 30-40% sidelap) and the minimum number of GCPs required for image matching was 10.

Intelligent Smart Farm A Study on Productivity: Focused on Tomato farm Households (지능형 스마트 팜 활용과 생산성에 관한 연구: 토마토 농가 사례를 중심으로)

  • Lee, Jae Kyung;Seol, Byung Moon
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.14 no.3
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    • pp.185-199
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    • 2019
  • Korea's facility horticulture has developed remarkably in a short period of time. However, in order to secure international competitiveness in response to unfavorable surrounding conditions such as high operating costs and market opening, it is necessary to diagnose the problems of facility horticulture and prepare countermeasures through analysis. The purpose of this study was to analyze the case of leading farmers by introducing information and communication technology (ICT) in hydroponic cultivation agriculture and horticulture, and to examine how agricultural technology utilizing smart farm and big data of facility horticulture contribute to farm productivity. Crop growth information gathering and analysis solutions were developed to analyze the productivity change factors calculated from hydroponics tomato farms and strawberry farms. The results of this study are as follows. The application range of the leaf temperature was verified to be variously utilized such as house ventilation in the facility, opening and closing of the insulation curtain, and determination of the initial watering point and the ending time point. Second, it is necessary to utilize water content information of crop growth. It was confirmed that the crop growth rate information can confirm whether the present state of crops is nutrition or reproduction, and can control the water content artificially according to photosynthesis ability. Third, utilize EC and pH information of crops. Depending on the crop, EC values should be different according to climatic conditions. It was confirmed that the current state of the crops can be confirmed by comparing EC and pH, which are measured from the supplied EC, pH and draining. Based on the results of this study, it can be confirmed that the productivity of smart farm can be affected by how to use the information of measurement growth.

Isolation and Characterization of a Novel Bacterium Burkholderia gladioli Bsp-1 Producing Alkaline Lipase

  • Zhu, Jing;Liu, Yanjing;Yanqin, Yanqin;Pan, Lixia;Li, Yi;Liang, Ge;Wang, Qingyan
    • Journal of Microbiology and Biotechnology
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    • v.29 no.7
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    • pp.1043-1052
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    • 2019
  • Active lipase-producing bacterium Burkholderia gladioli Bps-1 was rapidly isolated using a modified trypan blue and tetracycline, ampicillin plate. The electro-phoretically pure enzyme was obtained by purification using ethanol precipitation, ion-exchange chromatography, and gel filtration chromatography. The molecular weight was 34.6 kDa and the specific activity was determined to be 443.9 U/mg. The purified lipase showed the highest activity after hydrolysis with $p-NPC_{16}$ at a pH of 8.5 and $50^{\circ}C$, and the $K_m$, $k_{cat}$, and $k_{cat}/K_m$ values were 1.05 mM, $292.95s^{-1}$ and $279s^{-1}mM^{-1}$, respectively. The lipase was highly stable at $7.5{\leq}pH{\leq}10.0$. $K^+$ and $Na^+$ exerted activation effects on the lipase which had favorable tolerance to short-chain alcohols with its residual enzyme activity being 110% after being maintained in 30% ethanol for 1 h. The results demonstrated that the lipase produced by the strain B. gladioli Bps-1 has high enzyme activity and is an alkaline lipase. The lipase has promising chemical properties for a range of applications in the food-processing and detergent industries, and has particularly high potential for use in the manufacture of biodiesel.

The Study on the Elderly Homodialysis Patients' Transition of Health Condition and Health-related Quality of Life (혈액투석노인의 건강상태 변화인식과 건강관련 삶의 질에 관한 연구)

  • Shim, Ok Su
    • 한국노년학
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    • v.30 no.1
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    • pp.179-194
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    • 2010
  • The purpose of this study examines the elderly hemodialysis patients' Health-related quality in life(HRQL) and investigates the relationship between HRQL and transition of health condition. The subject were 443 elderly hemodialysis patients(over 65 years old) in Seoul and Gyeonggi province. The Health-related quality of life scale was used in this research, in which were analysed a wide range of statistical descriptive analysis, Pearson's correlation, reliability analysis, multiple regression and the Scheffe test. The results were as follows. The HRQL were statiscally significant difference by Gender, Educational level, Live with spouse, Best supporter, and Number of Disease. Also the factor related to transition of health condition was Number of Disease. The transition of health condition accounted for 13.9% of variance in HRQL and Number of Disease accounted for an additional 7.4% of HRQL. The factors, which influenced finally on the HRQL in the elderly hemodialysis patients, were transition of health condition and number of disease. In conclusion adequate hemodialysis treatment and social services should be provided in accordance with the factors affecting HRQL in order to improve the HRQL for elderly hemodialysis patients.

Feasibility Study on Integration of SSR Correction into Network RTK to Provide More Robust Service

  • Lim, Cheol-Soon;Park, Byungwoon;Kim, Dong-Uk;Kee, Chang-Don;Park, Kwan-Dong;Seo, Seungwoo;So, Hyoungmin;Park, Junpyo
    • Journal of Positioning, Navigation, and Timing
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    • v.7 no.4
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    • pp.295-305
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    • 2018
  • Network RTK is a highly practical technology that can provide high positioning accuracy at levels between cm~dm regardless of user location in the network by extending the available range of RTK using reference station network. In particular, unlike other carrier-based positioning techniques such as PPP, users are able to acquire high-accuracy positions within a short initialization time of a few or tens of seconds, which increases its value as a future navigation system. However, corrections must be continuously received to maintain a high level of positioning accuracy, and when a time delay of more than 30 seconds occurs, the accuracy may be reduced to the code-based positioning level of meters. In case of SSR, which is currently in the process of standardization for PPP service, the corrections by each error source are transmitted in different transmission intervals, and the rate of change of each correction is transmitted together to compensate the time delay. Using these features of SSR correction is expected to reduce the performance degradation even if users do not receive the network RTK corrections for more than 30 seconds. In this paper, the simulation data were generated from 5 domestic reference stations in Gunwi, Yeongdoek, Daegu, Gimcheon, and Yecheon, and the network RTK and SSR corrections were generated for the corresponding data and applied to the simulation data from Cheongsong reference station, assumed as the user. As a result of the experiment assuming 30 seconds of missing data, the positioning performance compensating for time delay by SSR was analyzed to be horizontal RMS (about 5 cm) and vertical RMS (about 8 cm), and the 95% error was 8.7 cm horizontal and 1cm vertical. This is a significant amount when compared to the horizontal and vertical RMS of 0.3 cm and 0.6 cm, respectively, for Network RTK without time delay for the same data, but is considerably smaller compared to the 0.5 ~ 1 m accuracy level of DGPS or SBAS. Therefore, maintaining Network RTK mode using SSR rather than switching to code-based DGPS or SBAS mode due to failure to receive the network RTK corrections for 30 seconds is considered to be favorable in terms of maintaining position accuracy and recovering performance by quickly resolving the integer ambiguity when the communication channel is recovered.

Three Dimensional Printing Technique and Its Application to Bone Tumor Surgery (3차원 프린팅 기술과 이를 활용한 골종양 수술)

  • Kang, Hyun Guy;Park, Jong Woong;Park, Dae Woo
    • Journal of the Korean Orthopaedic Association
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    • v.53 no.6
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    • pp.466-477
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    • 2018
  • Orthopaedics is an area where 3-dimensional (3D) printing technology is most likely to be utilized because it has been used to treat a range of diseases of the whole body. For arthritis, spinal diseases, trauma, deformities, and tumors, 3D printing can be used in the form of anatomical models, surgical guides, metal implants, bio-ceramic body reconstruction, and orthosis. In particular, in orthopaedic oncology, patients have a wide variety of tumor locations, but limited options for the limb salvage surgery have resulted in many complications. Currently, 3D printing personalized implants can be fabricated easily in a short time, and it is anticipated that all bone tumors in various surgical sites will be reconstructed properly. An improvement of 3D printing technology in the healthcare field requires close cooperation with many professionals in the design, printing, and validation processes. The government, which has determined that it can promote the development of 3D printing-related industries in other fields by leading the use of 3D printing in the medical field, is also actively supporting with an emphasis on promotion rather than regulation. In this review, the experience of using 3D printing technology for bone tumor surgery was shared, expecting orthopaedic surgeons to lead 3D printing in the medical field.

Effects of Respiratory Rehabilitation Training Using a Harmonica for Patients With Spinal Cord Injuries (하모니카를 활용한 호흡재활 훈련이 척수손상환자의 호흡기능에 미치는 영향)

  • Kim, Hyuk Gun;Kim, Min Seo;Lim, Han Mil;Joeng, So;Shin, Uk Ju
    • Journal of Music and Human Behavior
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    • v.15 no.2
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    • pp.23-39
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    • 2018
  • The purpose of this research was to investigate the effects of respiratory rehabilitation using a wind instrument for patients suffering from spinal cord injuries. From January 15, 2018 to April 15, 2018, we conducted ten 1-hour sessions of a harmonica program with eight patients with spinal cord injuries with average age of 37 years who could not perform abdominal breathing by themselves. We measured and compared patients' breathing capacity before and after the 10 sessions. Designed particularly for patients with spinal cord injuries resulting in a limited range of neck movement, the study used a 10-hole diatonic harmonica whose length was relatively short. For those patients who had difficulty using their hands, a harmonica holder was provided. Participants were trained to play simple tunes. They were guided to use abdominal breathing to make sounds, with emphasis on those parts requiring long and strong breathing. The results showed that for all eight patients both their breathing volume and their inspiratory volume increased following participation in the harmonica program. Also, the program had psychological benefits (e.g., more life satisfaction and less sadness) and additional physical benefits (e.g., less dizziness due to low blood pressure and better phlegm spitting). This study offers a unique way to help patients with spinal cord injuries to improve their breathing capacity, which may also be associated with a greater quality of life.

Triamcinolone Acetonide Injections for Lateral Malleolar Bursitis of the Ankle (Triamcinolone Acetonide 주사를 이용한 족관절 외과 점액낭염의 치료)

  • Woo, Seung Hun;Kim, Jung Shin;Son, Seung Min;Shin, Won Chul
    • Journal of Korean Foot and Ankle Society
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    • v.23 no.1
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    • pp.12-17
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    • 2019
  • Purpose: This study examined the clinical outcomes and usefulness of triamcinolone acetonide (TA) injections as an option in the conservative treatment of patients with lateral malleolar bursitis of the ankle. Materials and Methods: A total of 27 patients (27 ankles), in whom TA injection had been performed between March 2016 and June 2017, were reviewed retrospectively. After the aspiration of fluid in the lateral malleolar bursal sac, 1 mL (40 mg) of TA was injected into the malleolar bursal sac. After the injection, the ankle was compressed with an elastic cohesive bandage for 2 to 4 weeks. The clinical outcomes and side effects were evaluated at the following time points: 2 weeks, 4 weeks, 3 months, 6 months, and 1 year after TA injection therapy. The responses to treatment were assessed according to the degree of fluctuation, shrinkage of the bursal sac, and soft tissue swelling. Results: The mean age was 62.1 years (range, 41~81 years); there were 19 males and 8 females. Complete resolution was observed in 26 patients (96.3%) after the first or second application of a TA injection, and a partial response was observed in 1 patient (3.7%) after the first TA injection. The physical component scores of Medical Outcomes Study 36-item Short-Form Health Survey improved from 71.1 to 76.0 at the last follow-up (p=0.001). Associated complications were 1 patient (3.7%) with skin atrophy and 3 patients (11.1%) with transient hyperglycemia in diabetes mellitus. Conclusion: TA injection is a useful and safe procedure for patients not responding to the usual conservative treatment of lateral malleolar bursitis of the ankle.

The Location and Range of Goryeo Palace in Gangwha (고려 강도궁궐의 위치와 범위 검토)

  • Lee, Sang-jun
    • Korean Journal of Heritage: History & Science
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    • v.47 no.3
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    • pp.108-127
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    • 2014
  • Gangwha was a capital city of Goryeo, in spite of short-term occupation, where various national facilities such as palace stood in a row. Nevertheless, its historic importance has been undervalued owing to undiscovered palace. According to ${\ll}Goryeosa{\gg}$, palace in Gangwha was built in imitation of that of Gaegyeong. If it is true, the site of the old palace in Gaeseong as an original could give significant information for that of Gangwha. The distinct feature of Gaegyeong is that the palace was located in the west area of the centerline from north to south and pond lay in the east side. There is a mountain in the center of capital and two rivers runs along side the mountain. Gunggol at Gwancheong-ri is a best place where structural peculiarities of Gaegyeong can be applicable to Gangwha. There is an area of celadon distribution and is some topographic similarities such as central mountain, two rivers are quite similar. It is assumable that excavation result of no. 405 Gwancheongri reflects the function of east pond and thoroughfare responds to main street from north to south of Gaegyeong. After all the analysis from archaeological information, old maps, cadastral maps, topographic similarities and so on, Gunggol at Gwancheong-ri within an arc shape road is a most suitable place as palace in Gangwha.