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Variation of Amylose Content Using dsRNAi Vector by Targeting 3'-UTR Region of GBSSI Gene in Rice  

Park, Hyang-Mi (National Institute of Crop Science, RDA)
Choi, Man-Soo (National Institute of Crop Science, RDA)
Chun, Areum (National Institute of Crop Science, RDA)
Lee, Jeung-Heui (Chunchon sub-station, NICS, RDA)
Kim, Myeong-Ki (National Institute of Crop Science, RDA)
Kim, Yeon-Gyu (National Institute of Crop Science, RDA)
Shin, Dong-Bum (National Institute of Crop Science, RDA)
Lee, Jang-Yong (National Academy of Agricultural Science, RDA)
Kim, Yul-Ho (National Institute of Crop Science, RDA)
Publication Information
Korean Journal of Breeding Science / v.42, no.5, 2010 , pp. 515-524 More about this Journal
Abstract
The amylose content of starch is a major factor in the texture of cooked cereal grains. Therefore, down-regulation of amylose synthesis is one of the alternative method to improve eating quality of rice. We developed transgenic rice plants designed to suppress granule-bound starch synthase I(GBSSI) gene using RNA interference(RNAi) technology. Transgenic plants with RNAi vector containing the 3'-UTR region of GBSSI showed a lower amylose content in rice endosperm than that of wild-type. The range of amylose content was 5.9~9.0% in the transgenic plants, whereas that of wild-type was 17.7~18.0%. Transgenic rices showed the decrease of short chain and the increase of long chain by analyzing chain length distribution of amylopectin in the endosperm. In the SEM micrographs, we found that compound starch granules in whole grains of the wild-type rice were readily split during fracturing, while the starch granules in RNAi-transgenic lines showed small voluminous, non-angular rounded bodies.
Keywords
RNAi; GBSSI; Amylose content; Chain length distribution of amylopectin; Rice;
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