• The Korean Journal of Ecology
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• v.26 no.1
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• pp.5-12
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• 2003

Seed germination rate and seedling growth were measured on 6 different species(Phytolacca americana, Eupatorium rugosum, Rumex acetocella, Echinochloa crusgalli, Cassia mimosoides var. nomame, Setaria viridis) treated with leaf extract of E. rugosum. Total phenolic compound and heavy metal were analyzed on leaf and soil with and without E. rugosum. The growth of P. americana seedlings were stimulated by 10% and 25% of E. rugosum water extract treatment. The content of total phenolic compounds in soil was lower than that of leaf extract, and 25% was confirmed as threshold concentration in natural systems because the total phenolic compounds were not significantly different between the control soils and the soil treated with 10%, and 25% extract. Total phenolic compound concentrations of the leaf extracts were highest (1.66 mg/l) with E. rugosum grown under the Quercus forest canopy and lowest (1.09 mg/l) for the plant grown in the mixed forest edge. Leaf extracts of plants selected in different sampling sites (Forest interior, Forest edge, under Pinus Canopy and Quercus Canopy) were significant, while soil extracts were not. Seed germination of R. acetocella and S. viridis were significantly inhibited at over 50% concentrations of E. rugosum, but C. mimosoides var. nomame was not affected at any concentration. The radicle and shoot growth of the native species group were reduced two times more than those of the exotic species group by the treatment of extracts. Especially, the seed germination percentage and dry weight of E. rugosum were greater than those of the control group by treatments with extracts of 10% and 25%. Analysis of aqueous extracts from E. rugosum by HPLC identified 6 phenolic compounds: caffeic acid (460.9 mg/l), benzoic acid (109.7 mg/l), protocatechuic acid (7.3 mg/l), ρ-hydroquinone (6.0 mg/l), cinnamic acid (2.7 mg/l) and hydroquinone (0.23 mg/l). The seed germination of P. americana was also inhibited dramatically by protocatechuic acid and cinnamic acid even though the content of caffeic acid (460.9 mg/l) was the highest among analyzed phenolic compounds. The heavy metal content of soil without A. altissima was higher than that of soil with E. rugosum. Particularly, Al, Fe and Mn was considerably high and most of the heavy metal were accumulated in leaves where a high level of total phenolic compounds was found.

• Journal of Bio-Environment Control
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• v.21 no.4
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• pp.343-347
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• 2012

The purpose of this study was to examine the dwarfing effect in variable temperature treatments on 1-year-old and 3-year-old Chloranthus glaber. The plants grown in four difference growth chamber under a mean light intensity $500{\pm}20$ lux, RH of $40{\pm}5%$, and temperature of $5^{\circ}C$, $10^{\circ}C$, $15^{\circ}C$, and $20^{\circ}C$ for 120 days from January 3, 2011. And then they were moved into a glass house (50% shading). In 1-year-old seedlings, the rate of plant height elongation was lowest at $5^{\circ}C$, but plant growth was not good. The number of leaves and leaf width were highly increased by $10^{\circ}C$ treatment although the plant height was slightly increased. Also the plant height of $15^{\circ}C$ and $20^{\circ}C$ was decreased and defoliation was started from the bottom leaves. Thus, $10^{\circ}C$ treatment was thought to be the most appropriate for dwarfing effect of 1-year-old seedlings. In 3-year-old seedlings, the plant height tended to increase with temperature, and growth pattern showed a similar trend between $5^{\circ}C$ and $10^{\circ}C$, $15^{\circ}C$ and $20^{\circ}C$. The number of leaves increased the most at $5^{\circ}C$. Lateral branches per node were mainly occurred at $5^{\circ}C$ and $10^{\circ}C$, whereas they almost didn't happen at $15^{\circ}C$ and $20^{\circ}C$. Flowering rate at $5^{\circ}C$ and $10^{\circ}C$ reached more than 90% and 60%, respectively, but it was too low at $15^{\circ}C$ and $20^{\circ}C$ until the end of August. From these results, it could improve plant quality of 1-year-old and 3-year-old Chloranthus glaber making compact potted plants when temperatures were maintained at $10^{\circ}C$ and $5^{\circ}C$, respectively.

• Korean Journal of Environmental Agriculture
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• v.6 no.2
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• pp.39-45
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• 1987

A water culture experiment was conducted to investigate the effect of As content in a culture solution on the water status and growth of rice plants. Rice (Oryza sativa L. Line. Iri 316) seeds were germinated in bentonite and cultivated there for 30 days. Rice seedlings were transplanted into 3.5l pots containing the culture solution on May 1, 1985 and allowed to grow without As treatment for one month. Afterwards, they were grown in a culture solution maintaining the final concentration of As, 0, 1, 5, 10 and 15mg/1 renewing in the solution dissolved sodium arsenate at intervals of 3 to 7 days. Plants were cultivated in the green house during the growing period and harvested 60 days after As treatment. The results obtained were as follows: Transpiration of rice plants was decreased with the increase of the As level in the culture solution. Stomatal diffusive resistance and leaf temperature increased with increase of As levels though the humidity and the air flow rate in leaf decreased. Air flow rate, transpiration and stomatal diffusive resistance showed a highly significant correlation with As contents in shoots and roots of rice plants: Espally The air flow rate and transpiration revealed a significantly higher correlation with As contents in the root than that in the shoot, but diffusive resistance showed adverse tendency. High levels of As in the culture solution depressed plant height, no. of tillers, leaf width and dry weight of plant remarkably. Typical symptoms of As toxicity were root discoloration, and necrosis of leaf tips and margins, and leaf rolling during the sunny daytime were also another symptoms.

• Applied Microscopy
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• v.37 no.2
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• pp.83-91
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• 2007

The present study examined the epidermal changes of adhesive disks which occur during attachment in Parthenocissus tricuspidata using scanning and transmission electron microscopy. Several adhesive disks, each covered with a bract, develop from the shoot apical meristem during early development. In the initial stage, the adhesive disks are club-shaped and their upper and lower epidermis are indistinguishable. However, in the actively growing stage, they become spherical and both epidermis are clearly differentiated into the adventitious roots. Prior to wall attachment, the adhesive disks exhibit adaxial convex and abaxial concave shapes, and electron-dense substances are abundant in the vacuoles of epidermal cells. The peripheral area of the adhesive disk is adhered first to the wall surface, while the central area is drawn inward in a vacuum-like state during attachment. As the attachment progresses and the electron-dense substances continue to discharge, the upper and lower epidermis rapidly undergo deterioration and the disks shrink considerably. At this stage, structural changes of the lower epidermis occur much faster than in the upper one. The discharged substance is accumulated on the wall surface, and this aids the attachment of adhesive disks on the wall for long periods. In this manner, the shape and structure of the adhesive disk epidermis change drastically from initial growth to the mature stage. Further, the role of electron-dense substance and shrinkage of the disk during attachment has been discussed in Parthenocissus tricuspidata.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.26 no.1
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• pp.110-114
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• 1981

These experiments were carried out to define the effects of NAA, 2, 4-D and Benzyladenine on the callus induction and the organ differentiation from the explants and to find out the vegetative propagation method of Korean ginseng. The results obtained are summarized as follows; 1. NAA was significantly effective in forming roots from the ginseng stem segment and the number of roots was increased by increasing NAA concentration in the medium. The roots were formed from both distal and proximal ends of the ginseng stem segments grown on the medium containing more than 2mg/L of NAA. 2. The amount of callus growth increased proportionatly with NAA concentration in the range of 4.0mg per liter in the medium. The callus was easly induced from stem segment than leaf segment and 2, 4-D was more effective in callus induction and growth than NAA. 3. The benzyladenine showed the significant inhibition effect in forming roots from ginseng explant. The callus was not induced with BA alone, but in BA and 2, 4-D or BA and NAA added medium, the callus was easily induced and its growth was also accelerated. The interaction effects between 2, 4-D and BA on the callus induction and growth were significantly higher than those between NAA and BA. 4. As the ginseng embryos were cultured on the M.S. medium supplemented with 2mg per liter NAA, number of shoots was significantly increased and the percentage of embryo which had shown more than 4 shoots later was 22.2%. On the medium containing 8mg per liter NAA, the ginseng embryo showed the normal growth of shoots and leaves, but increased roots and callus induction on the basal part of shoots. 5. When the shoots with 3 leaflets were cut in 1.5cm long and grown on the Blayde's medium containing NAA 1.0mg per liter, roots were formed at the proximal end of shoot, and a new ginseng seedling was successfully obtained.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.55 no.2
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• pp.126-132
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• 2010

A high sugar rice cultivar 'Danmi' which has been recently developed and released in the Functional Crop Resource Development Division, Department of Functional Crop, National Institute of Crop Science, has high glucose and sucrose contents but has a poor seed development. This defect poses some problem in ensuring stable seedling establishment in the nursery bed. We examined the germination and seedling emergence characteristics of 'Danmi' and determined an optimum seeding rate for seedling establishment. 'Danmi' seeds has a light 1,000-grain weight of 18.2g compared with Ilmibyeo's 26.7g. The 97.4% of 'Danmi' seeds has specific gravity below 1.0 while only 2.0% of seeds was fully developed with specific gravity above 1.12. However, the reverse is true in the Ilmibyeo seed used as a control cultivar. Although the seed germination of Danmi was 89-91% regardless of seed specific gravity but at a slower rate than Ilmibyeo regardless of water soaking temperatures of $20-30^{\circ}C$ because of high seed amount of specific gravity below 1.0 in 'Danmi'. It took 2～4 days longer of seed soaking in the Danmi seeds than Ilmibyeo before germination. 'Danmi' has low normal seedling emergence rate than Ilmibyeo in the seedbed soil due to high percentage of abnormal seedlings like stunting and incomplete growth. Normal seedling emergence rate of 'Danmi' in the seedbed was 62.6～64.9% for 10-day old seedling and 83.5～86.7% for 30-day old seedling which is lower by 29.6～30.0% and 11.3～12%, respectively than Ilmibyeo. Although 'Danmi' has low normal seedling emergence rate, but it has greater seed number per weight basis. Therefore, based on the normal seedling number per unit area of Ilmibyeo(control cultivar) for seedling rate of 10-day and 30-day old seedlings, the recommended seeding rate of 'Danmi' for transplanting rice is 220g seeds for 10-day old seedling and 130g for 30-day old seedling per nursery box, which is equivalent to 1.5 times seed volumes of Ilmibyeo.

• The Korean Journal of Pesticide Science
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• v.7 no.3
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• pp.176-181
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• 2003

The leaching behaviour of [aniline-$^{14}C$]mefenacet in soil was investigated using glass columns (5 cm I.D. $\times$ 30 cm. H) packed with two types of soils with different physicochemical properties. $^{14}C$-Mefenacet (8.33 kBq) and mefenacet (in total, 1.05 mg/kg) were treated onto soil columns and rice plants (Oryza sativa L.) were grown for 17 weeks on these columns. Leachates from the columns were collected at the rate of 122.5 mL per week. $^{14}C$-Activities leached from soil A (OM, 3.1%; CEC, 86 mmol(+)/kg; texture, loam) columns with and without rice plants were 1.95 and 4.19% of the originally applied, whereas those from soil B (OM, 1.3%; CEC, 71 mmol(+)/kg; texture, loam) were 2.69 and 7.05%, respectively. These results indicated that larger amounts of $^{14}C$ were percolated from soil B with less organic matter and from the columns without vegetation. $^{14}C$-Activities absorbed by rice plants from soil A and B were 8.95 and 8.47%, respectively, most of which remained in the root and shoot excluding unhulled grains and ears without grains. $62\sim73%$ of the originally applied $^{14}C$ remained in the depth of $0\sim5cm$ in soil. The mass balance indicated that the losses by volatilization and/or mineralization amounted to $3.4\sim9.2%$ of the originally applied. $^{14}C$-Radioactivities in the aqueous phase of the leachates ranged from 59.4 to 97.7% of the radioactivities in leachates, showing the fast transformation of mefenacet to the polar metabolites.

• Korean Journal of Agricultural and Forest Meteorology
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• v.13 no.3
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• pp.115-122
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• 2011

The impacts of elevated temperature and $CO_2$ were studied on the seedlings of Maackia amurensis and Viburnum opulus var. calvescens. The seedlings were grown in controlled-environment growth chambers with four combinations of temperature and $CO_2$ treatments: $25^{\circ}C$ + ambient $CO_2$ (400 ppm), $25^{\circ}C$ + elevated $CO_2$ (800 ppm), $30^{\circ}C$ + ambient $CO_2$ (400 ppm), and $30^{\circ}C$ + elevated $CO_2$ (800 ppm). Under elevated temperature and $CO_2$ concentration, the dry weight decreased in seedlings of M. amurensis, but increased in seedlings of V. opulus var. calvescens. In addition, the shoot to root (S/R) ratio in M. amurensis reduced but that of V. opulus var. calvescens increased under elevated $CO_2$ concentration. The S/R ratios of two tree species increased under higher temperature. M. amurensis represented lower carboxylation efficiency under higher temperature and $CO_2$ concentration and that of V. opulus var. calvescens showed lower values under the only higher temperature. Photosynthetic pigment content of in the leaves of M. amurensis was lower under higher $CO_2$ concentration and higher under the increase of temperature, but that of V. V. opulus var. calvescens decreased according to the increase of temperature. Chlorophyll a/b ratios of M. amurensis and V. V. opulus var. calvescens decreased obviously with the increase of $CO_2$ concentration and temperature, respectively. In conclusion, the growth and physiological responses under the environmental changes such as temperature and $CO_2$ concentration depend on the tree species. Therefore, more studies are needed to predict the response of each tree species against the climate changes.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.34 no.2
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• pp.87-99
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• 2016

This study was carried out to characterize 127 recently developed cultivars of Rose of Sharon (Hibiscus spp.) in Korea and foreign Countries for landscape uses. The examined factors were growth characteristics such as tree height of a 1-year grafted plant(cm), plant type, growth habit, leaf characteristics such as shape and size, flower characteristics such as color, shape, size, and red eye during 2014 and 2015 for landscape uses. The results are obtained as follows; Hibiscus hybrid 'Daewangchun', 'Daeil', 'Lohengrin', 'Yeonam', 'Joomong', 'Jina', and H. syriacus 'Honggarosu' had vigorous shoot growth and tall tree size of 100cm. New Hibiscus cultivars, Hibiscus hybrid 'Daewangchun' and 'Daeil', having vigorous growth, uniform plant habit, upright, and compact branches were developed through interspecific crosses between H. syriacus 'Samchully' (♀) and H. sinosyriacus 'Seobong' (♂). This newly developed cultivar 'Daewangchun', 'Daeil', 'Lohengrin', 'Yeonam', 'Joomong', 'Jina', and H. syriacus 'Honggarosu' having tall vigorous growth d unique flower with long red eye can be used as street tree or specimen plant in landscape. Otherwise, H. syriacus 'Tamla', 'Melrose', 'Bidan', 'Hi Lea', 'Byeollee', 'Byunghwa', 'Mibak', 'Hanyang', 'Chungam', 'Lil Kim Violet', 'Jongmoo', 'Eunhasu', Hibiscus hybrid 'Saehanseo', and Hibiscus hybrid 'Yousoon' were selected as small tree of 30~39cm. They had dwarf form in tree seemed to be suitable for pot or flower bed planting on both indoor and outdoor conditions. H. syriacus 'Antong', 'Chungjo', and 'Lil Kim' were less than 30 cm of tree size. H. syriacus 'Antong', 'Chungjo', and 'Lil Kim', characterized by its compact, upright and outwardly spreading plant habit; freely branching habit; dark green-colored leaves; good garden performance. Therefore, the new cultivars with tall and small tree size were a promising cultivar as a ground covers or pot planting as woody landscape plant.

• Journal of Soil and Groundwater Environment
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• v.11 no.3
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• pp.43-51
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• 2006

Generally abandoned mine soils have serious problems for introducing vegetation such as nutrient deficiency, poor physical properties, and phytotoxicity due to high levels of heavy metals. It is required to improve soil amenity for revegetation. One of its strategies is using organic materials such as compost manure and sludge. The pot experiments was conducted to evaluate the effects of pig manure and municipal sewage sludge on revegetation of mining area soil surface with Artemisia princeps and Zoysia japonica. Application rate of pig manure and municipal sewage sludge was $75{\sim}225$ Mg/ha and $150{\sim}450$ Mg/ha, respectively. The results showed that the application of manure and sludge increased organic matter about two-fold and total nitrogen contents about five-fold of mine soil and improved the growth of plants in all treatments compared to the control. The result of plant tissue analysis showed that both plants accumulate Cd, Cu and Zn in root tissue rather than shoot tissues. Increased sludge application reduced Zn accumulation in both plant tissue. Sequential extraction results indicated that addition of soil amendment induced increment of organically bound fractions of Cu and Zn. Organically bound fraction of Zn was significantly increased from 7.84% to 13.58% in Artemisia princeps planted soil and from 7.84% to 14.16% in Zoysia japonica planted soil, thereby bioavailability of heavy metals was reduced. The results suggested that application of organic materials to mine soil can reduce phytotoxicity of heavy metals and be helpful in introducing successful revegetation.