• Journal of Life Science
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• v.31 no.4
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• pp.367-376
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• 2021

This study evaluated the changes in the pH, acidity, lactobacillus and total bacterial counts, and sensory evaluation of Dolsan leaf mustard Kimchi (DLMK) after incubation for 50 days at 4℃ in the presence of one of three lactic acid bacteria (LAB); Weissella kimchii (W.k.), Leuconostoc gelidum (L.g.), and Leuconostoc mesenteroides (L.m.). The pH ranged from 5.12 to 5.62 and the acidity varied from 0.62% to 3.77% upon the addition of a 2% salt solution to the DLMK prepared using the three LAB. Overall, the LAB counts decreased rapidly from day 10 to day 20 of fermentation, whereas the total bacterial count decreased during the 50 days of fermentation. The pH and acidity in the DLMK ranged from 5.22 to 5.61 and from 0.91% to 4.41%, respectively, in the absence of the 2% salt solution. The LAB count decreased until 20 days and then increased thereafter, whereas the total bacterial count decreased until 20 days and then increased until 50 days. The condiments treated with or without the 2% salt solution showed a gradual decrease in appearance, flavor, salinity, hot taste, and overall acceptability as fermentation time increased. In addition, DPPH radical scavenging activity was high for 2% W. k. and 2% L. m., and ACE inhibitory activity was the highest, at 70.38% in the marinated condiments fermented at 10℃ for 8 days. Thus, this condiment production method may be useful for DLMK and other Kimchi types.

• Animal Bioscience
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• v.34 no.6
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• pp.957-966
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• 2021

Objective: Ovarian follicular development, which dependent on the proliferation and differentiation of granulosa cells (GCs), is a complex biological process in which miRNA plays an important role. Our previous study showed that miR-458b-5p is associated with ovarian follicular development in chicken. The detailed function and molecular mechanism of miR-458b-5p in GCs is unclear. Methods: The luciferase reporter assay was used to verify the targeting relationship between miR-458b-5p and catenin beta-1 (CTNNB1), which is an important transcriptional regulatory factor of the Wnt/β-catenin pathway. The cell counting kit-8 (CCK-8) assay, flow cytometry with propidium iodide (PI) and annexin V-fluorescein isothiocyanate (FITC) labeling were applied to explore the effect of miR-458b-5p on proliferation, cell cycle and apoptosis of chicken GCs. Quantitative real-time polymerase chain reaction and Western blot were used to detect the mRNA and protein expression levels. Results: We demonstrated that the expression of miR-458b-5p and CTNNB1 showed the opposite relationship in GCs and theca cells of hierarchical follicles. The luciferase reporter assay confirmed that CTNNB1 is the direct target of miR-458b-5p. Using CCK-8 assay and flow cytometry with PI and Annexin V-FITC labeling, we observed that transfection with the miR-458b-5p mimics significantly reduced proliferation and has no effects on apoptosis of chicken GCs. In addition, miR-458b-5p decreased the mRNA and protein expression of CD44 molecule and matrix metallopeptidase 7, which are the downstream effectors of CTNNB1 in Wnt/β-Catenin pathway and play functional roles in cell proliferation. Conclusion: Taken together, the data indicate that miR-458b-5p regulates ovarian GCs proliferation through Wnt/β-catenin signaling pathway by targeting CTNNB1, suggesting that miR-458b-5p and its target gene CTNNB1 may potentially play a role in chicken ovarian follicular development.

• Animal Bioscience
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• v.34 no.6
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• pp.975-984
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• 2021

Objective: Inbreeding depression of reproduction is a major concern in the conservation of native chicken genetic resources. Here, based on the successful development of strongly inbred (Sinb) and weakly inbred (Winb) Langshan chickens, we aimed to evaluate inbreeding effects on reproductive traits and identify candidate genes involved in inbreeding depression of reproduction in Langshan chickens. Methods: A two-sample t-test was performed to estimate the differences in phenotypic values of reproductive traits between Sinb and Winb chicken groups. Three healthy chickens with reproductive trait values around the group mean values were selected from each of the groups. Differences in ovarian and hypothalamus transcriptomes between the two groups of chickens were analyzed by RNA sequencing (RNA-Seq). Results: The Sinb chicken group showed an obvious inbreeding depression in reproduction, especially for traits of age at the first egg and egg number at 300 days (p<0.01). Furthermore, 68 and 618 differentially expressed genes (DEGs) were obtained in the hypothalamus and ovary between the two chicken groups, respectively. In the hypothalamus, DEGs were mainly enriched in the pathways related to vitamin metabolism, signal transduction and development of the reproductive system, such as the riboflavin metabolism, Wnt signaling pathway, extracellular matrix-receptor interaction and focal adhesion pathways, including stimulated by retinoic acid 6, serpin family F member 1, secreted frizzled related protein 2, Wnt family member 6, and frizzled class receptor 4 genes. In the ovary, DEGs were significantly enriched in pathways associated with basic metabolism, including amino acid metabolism, oxidative phosphorylation, and glycosaminoglycan degradation. A series of key DEGs involved in folate biosynthesis (gamma-glutamyl hydrolase, guanosine triphosphate cyclohydrolase 1), oocyte meiosis and ovarian function (cytoplasmic polyadenylation element binding protein 1, structural maintenance of chromosomes 1B, and speedy/RINGO cell cycle regulator family member A), spermatogenesis and male fertility (prostaglandin D2 synthase 21 kDa), Mov10 RISC complex RNA helicase like 1, and deuterosome assembly protein 1) were identified, and these may play important roles in inbreeding depression in reproduction. Conclusion: The results improve our understanding of the regulatory mechanisms underlying inbreeding depression in chicken reproduction and provide a theoretical basis for the conservation of species resources.

• Journal of The Korean Astronomical Society
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• v.52 no.1
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• pp.23-30
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• 2019

The KVN(Korean VLBI Network)-style simultaneous multi-frequency receiving mode is demonstrated to be promising for mm-VLBI observations. Recently, other Very long baseline interferometry (VLBI) facilities all over the globe start to implement compatible optics systems. Simultaneous dual/multi-frequency VLBI observations at mm wavelengths with international baselines are thus possible. In this paper, we present the results from the first successful simultaneous 22/43 GHz dual-frequency observation with KaVA(KVN and VERA array), including images and astrometric results. Our analysis shows that the newly implemented simultaneous receiving system has brought a significant extension of the coherence time of the 43 GHz visibility phases along the international baselines. The astrometric results obtained with KaVA are consistent with those obtained with the independent analysis of the KVN data. Our results thus confirm the good performance of the simultaneous receiving systems for the nonKVN stations. Future simultaneous observations with more global stations bring even higher sensitivity and micro-arcsecond level astrometric measurements of the targets.

• Earthquakes and Structures
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• v.15 no.6
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• pp.667-685
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• 2018

In eccentrically braced steel frames (EBFs), the links are fuse members which enter inelastic phase before other structure members and dissipate the seismic energy. Based on the force-based seismic design method, damages and plastic deformations are limited to the links, and the main structure members are required tremendous sizes to ensure elastic with limited or no damage. Force-based seismic design method is very common and is found in most design codes, it is unable to determine the inelastic response of the structure and the damages of the members. Nowadays, methods of seismic design are emphasizing more on performance-based seismic design concept to have a more realistic assessment of the inelastic response of the structure. Links use ordinary steel Q345 (the nominal yielding strength $f_y{\geq}345MPa$) while other members use high strength steel (Q460 $f_y{\geq}460MPa$ or Q690 $f_y{\geq}690MPa$) in eccentrically braced frames with high strength steel combination (HSS-EBFs). The application of high strength steels brings out many advantages, including higher safety ensured by higher strength in elastic state, better economy which results from the smaller member size and structural weight as well as the corresponding welding work, and most importantly, the application of high strength steel in seismic fortification zone, which is helpful to popularize the extensive use of high strength steel. In order to comparison seismic behavior between HSS-EBFs and ordinary EBFs, on the basis of experimental study, four structures with 5, 10, 15 and 20 stories were designed by PBSD method for HSS-EBFs and ordinary EBFs. Nonlinear static and dynamic analysis is applied to all designs. The loading capacity, lateral stiffness, ductility and story drifts and failure mode under rare earthquake of the designs are compared. Analyses results indicated that HSS-EBFs have similar loading capacity with ordinary EBFs while the lateral stiffness and ductility of HSS-EBFs is lower than that of EBFs. HSS-EBFs and ordinary EBFs designed by PBSD method have the similar failure mode and story drift distribution under rare earthquake, the steel weight of HSS-EBFs is 10%-15% lower than ordinary EBFs resulting in good economic efficiency.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.6
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• pp.27-35
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• 2021

Generally, a proper evaluation method of the aging phenomenon of PV modules is required as the electrical performance and lifespan of PV modules can degrade significantly due to several environmental factors, while they are generally known as devices that are used semi-permanently for more than 20 years. On the other hand, there is a lack of objectivity in the existing evaluation method of the aging phenomenon, which compares the adjusted PV output based on STC with the initial PV module specifications due to the data distortion while adjusting the measured data. Therefore, this study implemented a test device for an aging diagnosis to measure and collect actual data from a PV module section and modeled the data for aging using MATLAB S/W to minimize the variability of the PV output, communication error, and delay. Furthermore, this study confirmed the usefulness of the presented test device for aging diagnosis of the PV modules by diagnosing the total period and yearly-basis degradation rate of aging PV modules as 25.73% and 1.55%, respectively, according to the on-site output characteristics of the PV modules by season.

• International Area Studies Review
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• v.21 no.2
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• pp.21-44
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• 2017

Under the new normal, the China's economy growth has changed rapid growth to moderate growth since 2007. With new paradigm, China is facing an abnormally severe employment situation. Also the financial expenditure is an important macro adjustment method. The research analyzes both implications of financial expenditures to employment in China, and the trend of implication in different regions. The research was conducted by 2SLS method using the panel data of 31 Chinese local governments(provinces, cities, and autonomous districts) during 1998 to 2015. The main findings are as follows. In the new normal model(2008-2015), the financial expenditure to urban employment have higher effect than total employment. Also, higher income region have more positive effect than lower income region. Medical, technology expenditure have positive effect to total employment, social security, education expenditure have positive effect to urban employment. In the total model(1998-2015) have similar results with new normal model, but the elasticity is more higher than total model. Ultimately, it can be seen that the efficiency of financial expenditure is lower than new normal model. The government should increase the proportion of expenditure in fields of social security, education, medical, technology, and improve the expenditure structure. So as to promote the effect of financial expenditure to employment in new normal economy.

• The Journal of Korean Philosophical History
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• no.53
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• pp.35-61
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• 2017

The formation process of Dasan(茶山)'s Yi Xue(易學) in Xinghuxuepai(星湖學派) was examined. Dasan(茶山)'s Yi Xue(易學) succeeded to the methods of Huti (互體) and Yaobian(爻變) from Seong-ho(星湖) and Ha-bin(河濱), and Dasan(茶山) thought that he couldn't cover Zhouyi("周易") just with this one method. So he suggested the four methods called Yilisifa(易理四法), and supplemented it with 18 methods through Duyiyaozhi(?讀易要旨?). Accordingly, the process of how Dasan(茶山)'s Yi Xue(易學) was established was examined one by one. Dasan(茶山) succeeded to the method of Huti(互體) from Seong-ho Lee Ik(星湖李瀷), the leading master of Xinghuxuepai(星湖學派). Nevertheless, Dasan(茶山) argued that the method of Huti(互體) had been from Zhu Xi(朱子). However, this argument of Dasan(茶山)'s seems to have resulted from Zhengzu(正祖)'s academic research policy. As a way to interpret Zhouyi(?周易?), Dasan(茶山) succeeded to the method of Yaobian(爻變) from Ha-bin(河濱). However, Dasan(茶山) argued that Yaobian(爻變)was Zhu Xi(朱子)'s intention. He had no choice but this because of Zhengzu(正祖)'s academic research policy. Dasan(茶山) succeeded to the method of Huti(互體) from Seong-ho(星湖) and Yaobian(爻變) from Ha-bin(河濱), but Dasan(茶山) proposed the four key methods called Yilisifa(易理四法). Many researchers have argued that, as for Dasan(茶山)'s modern viewpoint, Dasan(茶山) developed Yi Xue(易學) in a similar way as Xihe Mauqiling(西河 毛奇齡). However, this viewpoint cannot be judged to have been similar as it was expressed when Dasan(茶山) himself criticized Xihe(西河)'s Jingxueguan(經學觀).

• Animal Bioscience
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• v.34 no.7
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• pp.1202-1209
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• 2021

Objective: The intestinal microbiota plays an important role in host physiology, metabolism, immunity, and behavior. And host genetics could influence the gut microbiota of hybrid animals. The three-way cross model is commonly utilized in commercial pig production; however, the use of this model to analyse the gut microbial composition is rarely reported. Methods: Two three-way hybrid pigs were selected, with Saba pigs as the starting maternal pig: Duroc× (Berkshire×Saba) (DBS) pig, Berkshire×(Duroc×Saba) (BDS) pig. One hundred pigs of each model were reared from 35 days (d) to 210 d. The body weight or feed consumption of all pigs were recorded and their feed/gain (F/G) ratio was calculated. On day 210, 10 pigs from each three-way cross were selected for slaughter, and cecal chyme samples were collected for 16S rRNA gene sequencing. Results: The final body weight (FBW) and average daily gain (ADG) of DBS pigs were significantly higher than those of BDS pigs (p<0.05), while the F/G ratios of DBS pigs were significantly lower than those of BDS pigs (p<0.05). The dominant phyla in DBS and BDS pigs were Bacteroidetes (55.23% vs 59%, respectively) and Firmicutes (36.65% vs 34.86%, respectively) (p>0.05). At the genus level, the abundance of Prevotella, Roseburia, and Anaerovibrio in DBS pigs was significantly lower than in BDS pigs (p<0.01). The abundance of Eubacterium, Clostridium XI, Bacteroides, Methanomassiliicoccus, and Parabacteroides in DBS pigs was significantly higher than in BDS pigs (p<0.05). The FBWs and ADGs were positively correlated with Bacteroides, ClostridiumXI, and Parabacteroides but negatively correlated with the Prevotella, Prevotella/Bacteroides (P/B) ratio, Roseburia, and Anaerovibrio. Conclusion: These results indicated that host genetics affect the cecal microbiota composition and the porcine gut microbiota is associated with growth performance, thereby suggesting that gut microbiota composition may be a useful biomarker in porcine genetics and breeding.

• Animal Bioscience
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• v.34 no.7
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• pp.1105-1115
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• 2021

Objective: To conserve and utilize the genetic resources of a traditional Chinese indigenous pig breed, Liangshan pig, we assessed the genetic diversity, genetic structure, and genetic distance in this study. Methods: We used 50K single nucleotide polymorphism (SNP) chip for SNP detection of 139 individuals in the Liangshan Pig Conservation Farm. Results: The genetically closed conserved population consisted of five overlapping generations, and the total effective content of the population (Ne) was 15. The whole population was divided into five boar families and one non-boar family. Among them, the effective size of each generation subpopulation continuously decreased. However, the proportion of polymorphic markers (P_N) first decreased and then increased. The average genetic distance of these 139 Liangshan pigs was 0.2823±0.0259, and the average genetic distance of the 14 boars was 0.2723±0.0384. Thus, it can be deduced that the genetic distance changed from generation to generation. In the conserved population, 983 runs of homozygosity (ROH) were detected, and the majority of ROH (80%) were within 100 Mb. The inbreeding coefficient calculated based on ROH showed an average value of 0.026 for the whole population. In addition, the inbreeding coefficient of each generation subpopulation initially increased and then decreased. In the pedigree of the whole conserved population, the error rate of paternal information was more than 11.35% while the maternal information was more than 2.13%. Conclusion: This molecular study of the population genetic structure of Liangshan pig showed loss of genetic diversity during the closed cross-generation reproduction process. It is necessary to improve the mating plan or introduce new outside blood to ensure long-term preservation of Liangshan pig.