• Journal of Veterinary Clinics
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• v.16 no.2
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• pp.321-327
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• 1999

This study was performed to examine the clinical, serological, ultrasonographic and pathological findings in dogs with acute liver disease induced by tetrachlorethylene at 4 times of anthelminthic oral dose. The results obtained through this experiment could be summarized as follows: 1. The dogs administered with tetrachlorethylene, revealed decreased body weight, and showed lethargy and depression. 2. In serological findings, bilirubin values slightly increased, AST and ALT was decreased at 1∼3 days, and after that time increased according to the lapse of days, and revealed the highest at 5 days, and decreased to normal values at 6 days. 3. In ultrasonographic findings, branches of the portal vein were increased, the echodensity of the liver parenchyma was decreased at early stage, and increased at mid stage, and decreased at last stage. 4. In histopathological findings, necrosis of parenchymal cell, and perivascular hemorrhage were observed more severely at 6 days, as compared with 3 days. There results suggest that ultrasonographic examination is considered to be a more simple, rapid, non-invasive and useful diagnostic method for acute liver parenchymal lesion.

• Journal of Plant Biology
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• v.28 no.3
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• pp.207-216
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• 1985

Radial immunodiffusion, rocket immunoelectrophoresis, and Boyden procedure have been employed as quantitative analysis of pollen proteins in a systematic investigation of selected amentiferous plant taxa. Data presented here are continued and supplementary to the previous qualitative analysis of immunoprecipitin systems for the same purpose. Although the number of taxa tested has been limited, the serological evidence indicates that the Betulaceae has the greatest similarity to the Fagaceae, next to the Juglandaceae, the least to the Salicaceae, when antisera against Alnus hirsuta and Betula platyphylla var. japonica were used for tests. Within the Betulaceae Alnus and Betula show greatly similar affinities together, but less similar to the rest of genera: Carpinus, Carya and Corylus. When antisera against Quercus aliena, Q. dentata, and Q. glauca were used for tests, the following decreasing order to serological affinities was obtained: Quercus Alnus, Betula Carpinus, Carya, Corylus Juglans, Pterocarya Populus. Overall serological data come closer to supportint the classification systems of Cronquist, Takhtajan, and Hutchinson; but less of Thorne and Bessey. In addition this investigation indicated that pollen, with its high protein content, provided an excellent source of extractable antigens for serosystematic researches.

• Korean Journal of Veterinary Service
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• v.13 no.1
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• pp.64-68
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• 1990

This experiment was to investigate the Leptospiral antibody in the pigs with the serological test in two areas of Kangwondo from March to April. 1989. Twelve different living antigen (L. icterohaemorrhagiae, L. pomona, L. hardjo, L. australis, L. canicola, L. autumnalis, L. grippotyphosa, L. tarassovi, L. pyrogenes, L. bataviae, L. lai and L. ballum) of Leptospira interrogans was used for the serological test in the pigs. The blood sample of 280 pigs collected from the slaughter houses were detected by microscopic-agg-lutination test. The results were as follows, 1. Among the serum sample of 280 heads of the pigs, 6 heads of the swine (2.14%) were positive. 2. Among the positive sample of 6 heads, 3 heads of the swine (1.1%) showed the antibody of L. ict-erohaemorrhagiae, 2 heads of the swine (0.7%) showed the antibody of L. canicola, and 1 head of the swine (0.4%) showed the antibody of L. pomona. 3. The positive rate of Leptospira interrogans in the swine of Kangwondo appeared to be very low.

• Korean Journal of Veterinary Research
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• v.41 no.1
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• pp.67-71
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• 2001

A bacteriological and serological investigation was conducted in a commercial breeding kennel in Taegu city in which canine abortion caused by Brucella canis occurred, and the family dogs in this area were also surveyed for Br canis infection during a period from March 1999 to May 2000. Of 195 dogs in the breeding kennel, 50(25.6%) were found to be bacteremic and 82(42.1%) were shown to be positive for canine brucellosis by both bacteriological and serological test. Of 357 family dogs examined, 17(4.8%) had an agglutinin titer of 1 : 160 or higher. Of these 17 dogs, 5(2.8%) were from indoor dogs and 12(6.7%) were from outdoor dogs. Only 2 mongrel dogs(1 female and 1 male) of 17 serologically positive dogs showed an agglutinin titer of 1 : 2560 or higher and Br canis was isolated from their blood.

• Journal of the Korean Society of Tobacco Science
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• v.19 no.1
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• pp.5-10
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• 1997

Three strains of W isolated from tobacco, tomato and Pepper plants in Korea were characterized based on biological response, serological relationship, and peptide mapping of the capsid Proteins. The strains designated as TMV-common, TMV-Pepper, and TMV-tomato could be distinguishable by different visual symptoms on 3 varieties of tobacco, one variety of tomato and Pepper for each among 27 plant specieces. Serological relationships were examined by agar gel double diffusion test. Only traceable or weak reaction was observed in the incompatible antigen-antibody combinations. The Pepper strain, however, showed trace in reaction with other two antisera. Peptide maps of the capsid proteins digested by V8 protease or by trypsin were also distinguishable, suggesting differences in composition and/or sequence of the amino acids among the strains.

• Journal of the Korean Society of Tobacco Science
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• v.6 no.2
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• pp.141-146
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• 1984

Two PVY strains (PVY-VB and PVY-VN) isolated from tobacco in Korea were compared for their serological relationship with other 8 strains which were obtained from tobacco or potato in different countries. One of these strains, PVY-Argentina showed the spur reaction to PVY-VB and PVY-VN antisera in SDS-agar gel double diffusion plates. The two Korean PVY strains were closely related to other strains except for one, PVY-Argentina when antigen-antibody reciprocal absorption tests were conducted. It is suggested that the strain, PVY-Argentina, is a new serotype containing a specific antigenic site different from other 9 strains tested.

• Journal of Genetic Medicine
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• v.12 no.2
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• pp.100-108
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• 2015

Purpose: Conventional methods for the prenatal detection of fetal RhD status involve invasive procedures such as fetal blood sampling and amniocentesis. The identification of cell-free fetal DNA (cffDNA) in maternal plasma creates the possibility of determining fetal RhD status by analyzing maternal plasma DNA. However, some technical problems still exist, especially the lack of a positive control marker for the presence of fetal DNA. Therefore, we assessed the feasibility and accuracy of fetal RHD genotyping incorporating the RASSF1A epigenetic fetal DNA marker from cffDNA in the maternal plasma of RhD-negative pregnant women in Korea. Materials and Methods: We analyzed maternal plasma from 41 pregnant women identified as RhD-negative by serological testing. Multiplex real-time PCR was performed by amplifying RHD exons 5 and 7 and the SRY gene, with RASSF1A being used as a gender-independent fetal epigenetic marker. The results were compared with those obtained by postnatal serological analysis of cord blood and gender identification. Results: Among the 41 fetuses, 37 were RhD-positive and 4 were RhD-negative according to the serological analysis of cord blood. There was 100% concordance between fetal RHD genotyping and serological cord blood results. Detection of the RASSF1A gene verified the presence of cffDNA, and the fetal SRY status was correctly detected in all 41 cases. Conclusion: Noninvasive fetal RHD genotyping with cffDNA incorporating RASSF1A is a feasible, reliable, and accurate method of determining fetal RhD status. It is an alternative to amniocentesis for the management of RhD-negative women and reduces the need for unnecessary RhIG prophylaxis.

• Journal of Plant Biology
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• v.24 no.4
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• pp.217-231
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• 1981

This research was conducted to determine if the obtained serological data would reveal: (1) grouping among basic Quercus species, (2) the degree of correspondence within and among any detected grouping, and (3) the comparative value of new serological technique, "rocket immunoelectrophoresis". Overall quantitative and qualitative data indicate the genus Quercus is distantly separated from the Fagus. Within the Quercus, the subgenus Cyclobalanopsis is separable from the Lepidobalanus. But the elevation of the Cyclobalanopsis to the rank of a separate genus is not warranted. Antisera produced to the species belonging to Lepidobalanus gave strong reactions with other experimental species in the same subgenus. Particularly, Q. aliena, Q. donarium, and Q. serrata are tied up each other in terms of serological similarity. Rocket immunoelectrophoresis used in this systematic research proved to be a valuable new technique for systematic serology. To obtain the degree of protein similarity (serological correspondence) this technique was used in plant systematic research and determined by sum of total rocket heights. The rocket immunoelectrophoresis illustrated the measurement of individual immunoprecipitin systems identical or partly identical to those in the reference-reaction as quantitation of all available rocket heights.t heights.

• Journal of Veterinary Clinics
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• v.27 no.4
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• pp.421-426
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• 2010

In view of free from bluetongue (BT) in the domestic cattle population in Korea, the key of quarantine testing for BT virus (BTV) infection is detection of cattle previously exposed to the virus. The objective of this study was to estimate the probability of detecting a cattle infected with BTV using a stochastic modeling analysis of existing quarantine testing data. Three testing scenarios were considered in this study: serological testing of all animals in all imported lots (scenario 1), serological testing of a sample of cattle from all imported lots (scenario 2), and serological testing of 50% of imported lots (scenario 3). In scenario 2 and 3, it was assumed that cattle were sampled (sample size) within each lot to detect 5% of the cattle in each lot with a 95% confidence, taking into account diagnostic sensitivity of the ELISA (enzyme-linked immunosorbent assay). The model output was the total number of BTV-infected cattle and the prevalence of BTV infection in imported cattle from the US, Australia, Canada and Japan. Compared to the scenario 1, the probability of detecting a BTV-infected cattle was estimated to be 19% and 1.6% in scenario 2 and 3, respectively. Furthermore, the analyses showed a 95% confidence that BTV prevalence was less or equal to $9.7{\times}10^{-4}$ (median = $1.5{\times}10^{-5}$), indicating that, for the scenario 2 and 3 with serological testing for a sample of cattle, the risk of introducing an exotic strain of BTV into Korea through the importation of live cattle would not be acceptable.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.55 no.5
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• pp.688-696
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• 2022

Obesity could cause immune-physiological disorders in fish. Yet, little is known about the impact of obesity on stress and histological responses. This study aimed to determine histological and physiological changes in and vulnerability of overfeeding-induced obese rainbow trout Oncorhynchus mykiss exposed to stress condition. Control, intermediate and overfed groups were fed at 1.5, 2.5 and 3.09% of their body weight per day, respectively, for eight weeks. Weight gain, body mass index, hepatosomatic index and serological parameters, and histology of liver were measured in five fish from each group at week 0, 2, 4, and 8. At week 8, 20 fish from each group were exposed to heat stress by increasing water temperature at a rate of 3℃ per day from 15 to 25℃ and maintaining the final temperature for 10 days. Overall, overfed fish showed significantly higher weight gain, body mass index, and serological parameters than those of fish in the other groups. Fish in the overfed and intermediate groups displayed multifocal infiltration of inflammatory cells in hepatic parenchyma. Mortality rate and serological parameters of fish in the overfed group exposed to heat stress were significantly higher than those of fish in the other groups, indicating increased vulnerability to environmental stress.