• Korean Journal of Fisheries and Aquatic Sciences
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• v.50 no.5
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• pp.624-629
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• 2017

A rainbow trout Onchorhynchus mykiss farm located in Gangwon province, South Korea, experienced approximately 10% mortality in June 2017. Most diseased fish had a markedly distended, gas-filled stomach, and exhibited abnormal behavior at the water surface. In this study, we attempted to identify the cause of stomach distension syndrome in those rainbow trout. The stomach of most of the affected fish were full of unidentified gases and some exudate, and yeast was isolated from the stomach mucosa. Pure cultures of yeast were identified using a multilocus sequence typing scheme based on 18S rRNA, internal transcribed spacers, large subunit rRNA, and the gene encoding the largest subunit of RNA polymerase II (RPB1). The RPB1 gene sequences were compared with those of related species available in a database. The yeast was identified as Scheffersomyces coipomoensis (Candida coipomoensis) based on sequence analyses. This is the first study to reveal that Sch. coipomoensis is a potential causative agent of stomach distension syndrome in farmed rainbow trout. Our results will be helpful for future related studies, and indicate that farmers and stakeholders should observe this emerging disease closely.

• Biomedical Science Letters
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• v.25 no.4
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• pp.407-416
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• 2019

Recent years have seen an increase in the incidence of candidiasis caused by non-albicans Candida (NAC) species. In fact, C. glabrata is now second only to C. albicans as the most common cause of invasive candidiasis. Therefore, the rapid genotyping specifically for C. glabrata is required for early diagnosis and treatment of candidiasis. A number of genotyping assays have been developed to differentiate C. glabrata sequence types (STs), but they have several limitations. In the previous study, multi-locus sequence typing (MLST) has performed with a total of 101 C. glabrata clinical isolates to analyze the prevalent C. glabrata STs in Korea. A total of 11 different C. glabrata STs were identified and, among them, ST-138 was the most commonly classified. Thus, a novel probe-based quantitative PCR (qPCR) assay was developed and evaluated for rapid and accurate identification of the predominant C. glabrata ST-138 in Korea. Two primer pairs and hybridization probe sets were designed for the amplification of internal transcribed spacer 1 (ITS1) region and TRP1 gene. Analytical sensitivity of the probe-based qPCR assay was 100 ng to 10 pg and 100 ng to 100 pg (per 1 μL), which target ITS1 region and TRP1 gene, respectively. This assay did not react with any other Candida species and bacteria except C. glabrata. Of the 101 clinical isolates, 99 cases (98%) were concordant with MLST results. This novel probe-based qPCR assay proved to be rapid, sensitive, highly specific, reproducible, and cost-effective than other genotyping assay for C. glabrata ST-138 identification.

• Journal of Microbiology
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• v.44 no.6
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• pp.685-688
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• 2006

The aim of this study was to characterize the molecular features of serogroup C Neisseria meningitidis strains circulating in Beijing, China. Twenty out of 23 strains belonged to ST 4821. The causative serosubtype for meningococcal meningitis was P1.12-1,16-8. All of the strains expressed class 3 PorB protein. Among the five pulsed-field gel electrophoresis patterns observed, pattern III predominated.

• Annual Conference on Human and Language Technology
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• 2017.10a
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• pp.203-208
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• 2017

본 논문은 한글 자모 조합 임베딩을 이용하여 오타에 강건한 한국어 품사 태깅 시스템을 구축하는 방법에 대해 기술한다. 최근 딥 러닝 연구가 활발히 진행되면서 자질을 직접 추출해야 하는 기존의 기계학습 방법이 아닌, 스스로 자질을 찾아서 학습하는 딥 러닝 모델을 이용한 연구가 늘어나고 있다. 본 논문에서는 다양한 딥 러닝 모델 중에서 sequence labeling에 강점을 갖고 있는 bidirectional LSTM CRFs 모델을 사용하였다. 한국어 품사 태깅 문제에서 일반적으로 사용되는 음절 임베딩은 약간의 오타에도 품사 태깅 성능이 크게 하락하는 한계가 있었다. 따라서 이를 개선하기 위해 본 논문에서는 한글 자모 임베딩 값을 조합시킨 음절 임베딩 방식을 제안하였다. 강제로 오타를 발생시킨 테스트 집합에서 실험한 결과, 자모 조합 임베딩 기법이 word2vec 음절 임베딩 방식에 비해 형태소 분할은 0.9%, 품사 태깅은 3.5% 우수한 성능을 기록하였다.

• 한국어정보학회:학술대회논문집
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• 2017.10a
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• pp.203-208
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• 2017

본 논문은 한글 자모 조합 임베딩을 이용하여 오타에 강건한 한국어 품사 태깅 시스템을 구축하는 방법에 대해 기술한다. 최근 딥 러닝 연구가 활발히 진행되면서 자질을 직접 추출해야 하는 기존의 기계학습 방법이 아닌, 스스로 자질을 찾아서 학습하는 딥 러닝 모델을 이용한 연구가 늘어나고 있다. 본 논문에서는 다양한 딥 러닝 모델 중에서 sequence labeling에 강점을 갖고 있는 bidirectional LSTM CRFs 모델을 사용하였다. 한국어 품사 태깅 문제에서 일반적으로 사용되는 음절 임베딩은 약간의 오타에도 품사 태깅 성능이 크게 하락하는 한계가 있었다. 따라서 이를 개선하기 위해 본 논문에서는 한글 자모 임베딩 값을 조합시킨 음절 임베딩 방식을 제안하였다. 강제로 오타를 발생시킨 테스트 집합에서 실험한 결과, 자모 조합 임베딩 기법이 word2vec 음절 임베딩 방식에 비해 형태소 분할은 0.9%, 품사 태깅은 3.5% 우수한 성능을 기록하였다.

• Clinical and Experimental Pediatrics
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• v.53 no.2
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• pp.129-135
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• 2010

Human rhinoviruses (HRVs) is a nonenveloped, single stranded RNA virus belonging to the family Picornavirudae. Transmission by direct contact such as hand-to-hand, hand-to-nose, and hand-to-eye has been readily demonstrated in experimental settings. HRV are the most frequent causes of common cold infection, however, they are also known to replicate in the lower respiratory tract and associated with more severe respiratory illnesses such as asthma. New technique such as reverse transcriptase polymerase chain reaction and molecular typing in HRV has been developed and our understanding of the importance of these respiratory viruses. HRVs consisted of 101 serotypes that are classified into groups A and B according to sequence variations. And there is a newly identified set of HRVs, called Group C, and it is currently under investigation. In recent study using PCR techniques, HRVs accounted for approximate 50-80% of common colds and 85 % of childhood asthma exacerbations and in more than half of adult exacerbations. However, the mechanisms of HRV- induced asthma exacerbations are poorly understood. This review discusses the association between HRVs and childhood asthma.

• Mycobiology
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• v.48 no.2
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• pp.133-138
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• 2020

Talaromyces halophytorum sp. nov. was isolated from the roots of halophyte Limonium tetragonum collected from Seocheon-gun, Korea in November 2015. It showed a slow growth on yeast extract sucrose agar at 25 ℃, no growth at 4 ℃ or 37 ℃ and produced smooth-walled and globose to sub-globose conidia. T. halophytorum is phylogenetically distinct from the other reported Talaromyces species of section Trachyspermi based on multi-locus sequence typing results using partial fragments of β-tubulin, calmodulin, ITS, and RNA polymerase II genes.

• Mycobiology
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• v.50 no.3
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• pp.155-165
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• 2022

Penicillium vietnamense sp. nov. was isolated from Nha Trang Bay, Vietnam in June 2017. It is phylogenetically distinct from the sister species of Penicillium section Charlesia series Indica based on multi-locus sequence typing results using internal transcribed spacer, large subunit ribosomal RNA, b-tubulin, calmodulin, and RNA polymerase II second largest subunit regions. It showed strong growth on Czapek yeast autolysate agar at 37 ℃, a strong acid production on Creatine sucrose agar, and produced short stipes, small vesicles, and subglobose to globose conidia delicately roughened with very short ridges. As the first novel marine fungi species described from Vietnam and discovered in a unique environment, the data could be significant for understanding the taxonomy and geographical distribution of marine fungi in tropical coastal systems such as Vietnam.

• Animal cells and systems
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• v.1 no.1
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• pp.129-134
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• 1997

HLA-A2 is one of the most diversified HLA-class I antigen with 17 subtypes so far identified at the molecular level. HLA-A*02 subtyping has significant implications on the tissue typing for organ and bone marrow transplantations. Recently, DNA-based typing methods have been successfully applied to the elucidation of HLA gene polymorphisms. In the present study, HLA-A*O2 genotyping was established by using nested polymerase chain reaction-sequence specific primers (PCR-SSP) and distribution of A*O2 alleles were determined in Korean individuals. Genomic DNA prepared from four B-lymphoblastoid cell lines and lymphocytes from serologically defined 48 HLA-A2 Korean individuals by phenol/chloroform extractions was typed. The results of the four B-lymphoblastoid cells were consistent with the previous data typed by PCR analysis. Five A*O2 alleles-A*0201, A*0203, A*0206, A*0207 and A*0210-were commonly observed in a total of 17 A*02 alleles. Of these, A*0207 (f=49.0%) was the most frequent allele in Korean population. A*0206 (f=28.3%) and A*0201 (f=17.0%) were also found frequently while A*0203 and A*0210 types were observed in less than 5%. In conclusion, the high level of discrimination for HLA-A*O2 alleles will prove useful and informative in the study of transplant survival, and may identify the importance of allelic differences not readily detectable by serology on host and donor compatibility.

• International Journal of Industrial Entomology and Biomaterials
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• v.28 no.2
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• pp.39-50
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• 2014

In order to understand the diversity and genetic relationships of silkworm strains preserved in Korea, we genotyped 78 Bombyx mori strains (Bombycidae: Lepidoptera) originating from Japan, using eight polymorphic microsatellite loci. We obtained per-locus allele numbers ranging from 5 to 16 (with an average value of 9.1), per-locus observed heterozygosity ranging from 0.13 to 1.00, and per-locus polymorphic information content ranging from 0.36 to 0.77, indicating that some loci are highly variable. Phylogenetic analysis with the eight concatenated microsatellite loci showed no clustering based on known strain characteristics and origin. Nineteen strain-specific apomorphic alleles, which discriminated 16 of the 78 silkworm strains, were obtained from eight loci. These strain-specific alleles can thus be utilized for routine discrimination of strains from Japan, without any further typing of other loci. Homozygotes were also observed at some loci (27 of 118 genotypes), which can also be used to discriminate several strains by typing a few loci. These results showed that eight microsatellite loci described herein were sufficiently variable to discriminate among the 78 silkworm strains we examined, and may be useful for future investigations of this economically important species.