Mycobiology

The Korean Society of Mycology (한국균학회)
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Penicillium vietnamense sp. nov., the First Novel Marine Fungi Species Described from Vietnam with a Unique Conidiophore Structure and Molecular Phylogeny of Penicillium Section Charlesia

  • Nguyen, Van Duy (Institute of Biotechnology and Environment, Nha Trang University) ;
  • Pham, Thu Thuy (Institute of Biotechnology and Environment, Nha Trang University)
  • Received : 2021.07.21
  • Accepted : 2022.04.18
  • Published : 2022.08.31
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Abstract

Penicillium vietnamense sp. nov. was isolated from Nha Trang Bay, Vietnam in June 2017. It is phylogenetically distinct from the sister species of Penicillium section Charlesia series Indica based on multi-locus sequence typing results using internal transcribed spacer, large subunit ribosomal RNA, b-tubulin, calmodulin, and RNA polymerase II second largest subunit regions. It showed strong growth on Czapek yeast autolysate agar at 37 ℃, a strong acid production on Creatine sucrose agar, and produced short stipes, small vesicles, and subglobose to globose conidia delicately roughened with very short ridges. As the first novel marine fungi species described from Vietnam and discovered in a unique environment, the data could be significant for understanding the taxonomy and geographical distribution of marine fungi in tropical coastal systems such as Vietnam.

Keywords

Acknowledgement

The authors acknowledge Tran Chau Loan at Nha Trang University (NTU) for technical support.

References

  1. Biourge P. Les moissisures du groupe Penicillium link. La Cellule. 1923;33:7-331.
  2. Samson RA, Yilmaz N, Houbraken J, et al. Phylogeny and nomenclature of the genus Talaromyces and taxa accommodated in Penicillium subgenus Biverticillium. Stud Mycol. 2011;70(1):159-183. https://doi.org/10.3114/sim.2011.70.04
  3. Visagie CM, Houbraken J, Frisvad JC, et al. Identification and nomenclature of the genus Penicillium. Stud Mycol. 2014;78:343-371. https://doi.org/10.1016/j.simyco.2014.09.001
  4. Hou X, Zhang X, Xue M, et al. Recent advances in sorbicillinoids from fungi and their bioactivities (covering 2016-2021). J Fungi. 2022;8(1):62. https://doi.org/10.3390/jof8010062
  5. Klas KR, Kato H, Frisvad JC, et al. Structural and stereochemical diversity in prenylated indole alkaloids containing the bicyclo[2.2.2]diazaoctane ring system from marine and terrestrial fungi. Nat Prod Rep. 2018;35(6):532-558. https://doi.org/10.1039/C7NP00042A
  6. Li K, Chen S, Pang X, et al. Natural products from mangrove sediments-derived microbes: structural diversity, bioactivities, biosynthesis, and total synthesis. Eur J Med Chem. 2022;230:114117. https://doi.org/10.1016/j.ejmech.2022.114117
  7. Liu S, Su M, Song SJ, et al. Marine-derived Penicillium species as producers of cytotoxic metabolites. Mar Drugs. 2017;15(10):329. https://doi.org/10.3390/md15100329
  8. Wang X, Li Y, Zhang X, et al. Structural diversity and biological activities of the cyclodipeptides from fungi. Molecules. 2017;22(12):2026. https://doi.org/10.3390/molecules22122026
  9. Peterson SW. 2000. Phylogenetic analysis of Penicillium species based on ITS and LSU-rDNA nucleotide sequences. In: Samson RA, Pitt JI, editors. Integration of modern taxonomic methods for Penicillium and Aspergillus classification. Amsterdam (The Netherlands): Harwood Academic Publishers; p. 163-178.
  10. Peterson SW. Multilocus DNA sequence analysis shows that Penicillium biourgeianum is a distinct species closely related to P. brevicompactum and P. olsonii. Mycol Res. 2004;108(4):434-440. https://doi.org/10.1017/S0953756204009761
  11. Pham TT, Dinh VK, Nguyen VD. Biodiversity and enzyme activity of marine fungi with 28 new records from the tropical coastal ecosystems in Vietnam. Mycobiology. 2021;49(6):559-581. https://doi.org/10.1080/12298093.2021.2008103
  12. O'Callaghan B. 2008. Vietnam: a collaborative approach to MPA development. Gland (Switzerland): The World Conservation Union.
  13. Dung LD. Nha Trang Bay marine protected area, Vietnam: initial trends in coral structure and some preliminary linkages between these trends and human activities (2002-2005). Aquat Ecosyst Health Manag. 2009;12(3):249-257. https://doi.org/10.1080/14634980903146767
  14. Girich EV, Yurchenko AN, Smetanina OF, et al. Neuroprotective metabolites from Vietnamese marine derived fungi of Aspergillus and Penicillium genera. Mar Drugs. 2020;18(12):608. https://doi.org/10.3390/md18120608
  15. Latypov YY. Some data on the composition and structure of coral communities in the Littoral and sublittoral in the province of Khanh Hoa, Vietnam. J Mar Sci Res Dev. 2014;4:1.
  16. Nguyen VD, Le MH, Trang ST. 2013. Application of probiotics from marine microbes for sustainable marine aquaculture development. In: Kim S-K, editor. Marine microbiology: bioactive compounds and biotechnological applications. Weinheim (Germany): Wiley; p. 307-349.
  17. Pham TT, Ho THN, Nguyen VD. Screening for bacteriocin-like antimicrobial activity against shrimp pathogenic vibrios and molecular identification of marine bacteria from otter clam Lutraria philippinarum. Thai J Vet Med. 2014;1:345-353. https://doi.org/10.56808/2985-1130.2581
  18. Li L, Singh P, Liu Y, et al. Diversity and biochemical features of culturable fungi from the coastal waters of Southern China. AMB Express. 2014;4:60. https://doi.org/10.1186/s13568-014-0060-9
  19. Pitt JI. 1979. The genus Penicillium, and its teleomorphic states eupenicillium, and talaromyces. London (UK): Academic Press.
  20. Choi DH, You YH, Lee IS, et al. Penicillium ulleungdoense sp. nov. from Ulleung Island in Korea. Mycobiology. 2020;49(1):46-53. https://doi.org/10.1080/12298093.2020.1852702
  21. Toju H, Tanabe AS, Yamamoto S, et al. High-coverage ITS primers for the DNA-based identification of ascomycetes and basidiomycetes in environmental samples. PLoS One. 2012;7(7):e40863. https://doi.org/10.1371/journal.pone.0040863
  22. White TJ, Bruns T, Lee S. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Shinsky TJ, White TJ, editors. PCR protocols: a guide to methods and applications. New York (NY): Academic Press Inc.; p. 315-322.
  23. Kurtzman CP, Robnett CJ. Identification of clinically important ascomycetous yeasts based on nucleotide divergence in the 5' end of the large-subunit (26S) ribosomal DNA gene. J Clin Microbiol. 1997;35(5):1216-1223. https://doi.org/10.1128/jcm.35.5.1216-1223.1997
  24. Glass NL, Donaldson GC. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol. 1995;61(4):1323-1330. https://doi.org/10.1128/aem.61.4.1323-1330.1995
  25. Houbraken J, Spierenburg H, Frisvad JC. Rasamsonia, a new genus comprising thermotolerant and thermophilic Talaromyces and Geosmithia species. Antonie Van Leeuwenhoek. 2012;101(2):403-421. https://doi.org/10.1007/s10482-011-9647-1
  26. Kumar S, Stecher G, Li M, et al. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol. 2018;35(6):1547-1549. https://doi.org/10.1093/molbev/msy096
  27. Larkin MA, Blackshields G, Brown NP, et al. Clustal W and clustal X version 2.0. Bioinformatics. 2007;23(21):2947-2948. https://doi.org/10.1093/bioinformatics/btm404
  28. Saitou N, Nei M. The Neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987;4(4):406-425.
  29. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution. 1985;39(4):783-791. https://doi.org/10.2307/2408678
  30. Tamura K. Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G+C-content biases. Mol Biol Evol. 1992;9:678-687.
  31. Tamura K, Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol. 1993;10(3):512-526.
  32. Houbraken J, Kocsube S, Visagie CM, et al. Classification of Aspergillus, Penicillium, Talaromyces and related genera (Eurotiales): an overview of families, genera, subgenera, sections, series and species. Stud Mycol. 2020;95:5-169. https://doi.org/10.1016/j.simyco.2020.05.002
  33. Crous PW, Wingfield MJ, Lombard L, et al. Fungal planet description sheets: 951-1041. Persoonia. 2019;43:223-425. https://doi.org/10.3767/persoonia.2019.43.06
  34. Thom C. Cultural studies of species of Penicillium. USDA Bur Animal Ind Bull. 1910;118:1-107.
  35. Sandhu DK, Sandhu RS. A new species of Penicillium isolated from sputum. Can J Bot. 1963;41(8):1273-1274. https://doi.org/10.1139/b63-105
  36. Crous PW, Carnegie AJ, Wingfield MJ, et al. Fungal planet description sheets: 868-950. Persoonia. 2019;42:291-473. https://doi.org/10.3767/persoonia.2019.42.11
  37. Martinez AT, Calvo MA, Ramirez C. Scanning electron microscopy of Penicillium conidia. Antonie Van Leeuwenhoek. 1982;48(3):245-255. https://doi.org/10.1007/BF00400384
  38. Agurell SL. Costaclavine from Penicillium chermesinum. Experientia. 1964;20(1):25-26. https://doi.org/10.1007/BF02146022
  39. Hwu L, Cho C-J, Tzeanz S. Nucleotide sequence and the action of ribotoxin gene (sar gene) of Penicillium isolates from Taiwan. Bot Bull Acad Sinica. 2001;42:101-107.
  40. Huang H, Feng X, Xiao Z, et al. Azaphilones and p-terphenyls from the mangrove endophytic fungus Penicillium chermesinum (ZH4-E2) isolated from the South China sea. J Nat Prod. 2011;74(5):997-1002. https://doi.org/10.1021/np100889v
  41. Darsih C, Prachyawarakorn V, Wiyakrutta S, et al. Cytotoxic metabolites from the endophytic fungus Penicillium chermesinum: discovery of a cysteine-targeted Michael acceptor as a pharmacophore for fragment-based drug discovery, bioconjugation and click reactions. RSC Adv. 2015;5(86):70595-70603. https://doi.org/10.1039/C5RA13735G
  42. Liu H, Li X-M, Liu Y, et al. Meroterpenoids with a drimane-type spirosesquiterpene skeleton from the marine algal-derived endophytic fungus Penicillium chermesinum EN-480. J Nat Prod. 2016;79(4):806-811. https://doi.org/10.1021/acs.jnatprod.5b00893
  43. Amend A, Burgaud G, Cunliffe M, et al. Fungi in the marine environment: open questions and unsolved problems. mBio. 2019;10(2):e01189.
  44. Le H, Do Q, Doan M, et al. Chemical composition and biological activities of metabolites from the marine fungi Penicillium sp. isolated from sediments of Co To Island, Vietnam. Molecules. 2019;24(21):3830. https://doi.org/10.3390/molecules24213830