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http://dx.doi.org/10.1080/12298093.2022.2068750

Penicillium vietnamense sp. nov., the First Novel Marine Fungi Species Described from Vietnam with a Unique Conidiophore Structure and Molecular Phylogeny of Penicillium Section Charlesia  

Nguyen, Van Duy (Institute of Biotechnology and Environment, Nha Trang University)
Pham, Thu Thuy (Institute of Biotechnology and Environment, Nha Trang University)
Publication Information
Mycobiology / v.50, no.3, 2022 , pp. 155-165 More about this Journal
Abstract
Penicillium vietnamense sp. nov. was isolated from Nha Trang Bay, Vietnam in June 2017. It is phylogenetically distinct from the sister species of Penicillium section Charlesia series Indica based on multi-locus sequence typing results using internal transcribed spacer, large subunit ribosomal RNA, b-tubulin, calmodulin, and RNA polymerase II second largest subunit regions. It showed strong growth on Czapek yeast autolysate agar at 37 ℃, a strong acid production on Creatine sucrose agar, and produced short stipes, small vesicles, and subglobose to globose conidia delicately roughened with very short ridges. As the first novel marine fungi species described from Vietnam and discovered in a unique environment, the data could be significant for understanding the taxonomy and geographical distribution of marine fungi in tropical coastal systems such as Vietnam.
Keywords
DNA sequencing; marine fungi; morphology; phylogeny; scanning electron microscopy;
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1 O'Callaghan B. 2008. Vietnam: a collaborative approach to MPA development. Gland (Switzerland): The World Conservation Union.
2 Dung LD. Nha Trang Bay marine protected area, Vietnam: initial trends in coral structure and some preliminary linkages between these trends and human activities (2002-2005). Aquat Ecosyst Health Manag. 2009;12(3):249-257.   DOI
3 Latypov YY. Some data on the composition and structure of coral communities in the Littoral and sublittoral in the province of Khanh Hoa, Vietnam. J Mar Sci Res Dev. 2014;4:1.
4 Nguyen VD, Le MH, Trang ST. 2013. Application of probiotics from marine microbes for sustainable marine aquaculture development. In: Kim S-K, editor. Marine microbiology: bioactive compounds and biotechnological applications. Weinheim (Germany): Wiley; p. 307-349.
5 Visagie CM, Houbraken J, Frisvad JC, et al. Identification and nomenclature of the genus Penicillium. Stud Mycol. 2014;78:343-371.   DOI
6 Hou X, Zhang X, Xue M, et al. Recent advances in sorbicillinoids from fungi and their bioactivities (covering 2016-2021). J Fungi. 2022;8(1):62.   DOI
7 Li K, Chen S, Pang X, et al. Natural products from mangrove sediments-derived microbes: structural diversity, bioactivities, biosynthesis, and total synthesis. Eur J Med Chem. 2022;230:114117.   DOI
8 Liu S, Su M, Song SJ, et al. Marine-derived Penicillium species as producers of cytotoxic metabolites. Mar Drugs. 2017;15(10):329.   DOI
9 Peterson SW. 2000. Phylogenetic analysis of Penicillium species based on ITS and LSU-rDNA nucleotide sequences. In: Samson RA, Pitt JI, editors. Integration of modern taxonomic methods for Penicillium and Aspergillus classification. Amsterdam (The Netherlands): Harwood Academic Publishers; p. 163-178.
10 Peterson SW. Multilocus DNA sequence analysis shows that Penicillium biourgeianum is a distinct species closely related to P. brevicompactum and P. olsonii. Mycol Res. 2004;108(4):434-440.   DOI
11 Li L, Singh P, Liu Y, et al. Diversity and biochemical features of culturable fungi from the coastal waters of Southern China. AMB Express. 2014;4:60.   DOI
12 Pitt JI. 1979. The genus Penicillium, and its teleomorphic states eupenicillium, and talaromyces. London (UK): Academic Press.
13 Houbraken J, Spierenburg H, Frisvad JC. Rasamsonia, a new genus comprising thermotolerant and thermophilic Talaromyces and Geosmithia species. Antonie Van Leeuwenhoek. 2012;101(2):403-421.   DOI
14 Toju H, Tanabe AS, Yamamoto S, et al. High-coverage ITS primers for the DNA-based identification of ascomycetes and basidiomycetes in environmental samples. PLoS One. 2012;7(7):e40863.   DOI
15 White TJ, Bruns T, Lee S. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Shinsky TJ, White TJ, editors. PCR protocols: a guide to methods and applications. New York (NY): Academic Press Inc.; p. 315-322.
16 Glass NL, Donaldson GC. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol. 1995;61(4):1323-1330.   DOI
17 Larkin MA, Blackshields G, Brown NP, et al. Clustal W and clustal X version 2.0. Bioinformatics. 2007;23(21):2947-2948.   DOI
18 Saitou N, Nei M. The Neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987;4(4):406-425.
19 Tamura K. Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G+C-content biases. Mol Biol Evol. 1992;9:678-687.
20 Tamura K, Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol. 1993;10(3):512-526.
21 Martinez AT, Calvo MA, Ramirez C. Scanning electron microscopy of Penicillium conidia. Antonie Van Leeuwenhoek. 1982;48(3):245-255.   DOI
22 Crous PW, Wingfield MJ, Lombard L, et al. Fungal planet description sheets: 951-1041. Persoonia. 2019;43:223-425.   DOI
23 Thom C. Cultural studies of species of Penicillium. USDA Bur Animal Ind Bull. 1910;118:1-107.
24 Crous PW, Carnegie AJ, Wingfield MJ, et al. Fungal planet description sheets: 868-950. Persoonia. 2019;42:291-473.   DOI
25 Hwu L, Cho C-J, Tzeanz S. Nucleotide sequence and the action of ribotoxin gene (sar gene) of Penicillium isolates from Taiwan. Bot Bull Acad Sinica. 2001;42:101-107.
26 Huang H, Feng X, Xiao Z, et al. Azaphilones and p-terphenyls from the mangrove endophytic fungus Penicillium chermesinum (ZH4-E2) isolated from the South China sea. J Nat Prod. 2011;74(5):997-1002.   DOI
27 Liu H, Li X-M, Liu Y, et al. Meroterpenoids with a drimane-type spirosesquiterpene skeleton from the marine algal-derived endophytic fungus Penicillium chermesinum EN-480. J Nat Prod. 2016;79(4):806-811.   DOI
28 Amend A, Burgaud G, Cunliffe M, et al. Fungi in the marine environment: open questions and unsolved problems. mBio. 2019;10(2):e01189.
29 Le H, Do Q, Doan M, et al. Chemical composition and biological activities of metabolites from the marine fungi Penicillium sp. isolated from sediments of Co To Island, Vietnam. Molecules. 2019;24(21):3830.   DOI
30 Darsih C, Prachyawarakorn V, Wiyakrutta S, et al. Cytotoxic metabolites from the endophytic fungus Penicillium chermesinum: discovery of a cysteine-targeted Michael acceptor as a pharmacophore for fragment-based drug discovery, bioconjugation and click reactions. RSC Adv. 2015;5(86):70595-70603.   DOI
31 Biourge P. Les moissisures du groupe Penicillium link. La Cellule. 1923;33:7-331.
32 Samson RA, Yilmaz N, Houbraken J, et al. Phylogeny and nomenclature of the genus Talaromyces and taxa accommodated in Penicillium subgenus Biverticillium. Stud Mycol. 2011;70(1):159-183.   DOI
33 Klas KR, Kato H, Frisvad JC, et al. Structural and stereochemical diversity in prenylated indole alkaloids containing the bicyclo[2.2.2]diazaoctane ring system from marine and terrestrial fungi. Nat Prod Rep. 2018;35(6):532-558.   DOI
34 Wang X, Li Y, Zhang X, et al. Structural diversity and biological activities of the cyclodipeptides from fungi. Molecules. 2017;22(12):2026.   DOI
35 Choi DH, You YH, Lee IS, et al. Penicillium ulleungdoense sp. nov. from Ulleung Island in Korea. Mycobiology. 2020;49(1):46-53.   DOI
36 Pham TT, Dinh VK, Nguyen VD. Biodiversity and enzyme activity of marine fungi with 28 new records from the tropical coastal ecosystems in Vietnam. Mycobiology. 2021;49(6):559-581.   DOI
37 Girich EV, Yurchenko AN, Smetanina OF, et al. Neuroprotective metabolites from Vietnamese marine derived fungi of Aspergillus and Penicillium genera. Mar Drugs. 2020;18(12):608.   DOI
38 Pham TT, Ho THN, Nguyen VD. Screening for bacteriocin-like antimicrobial activity against shrimp pathogenic vibrios and molecular identification of marine bacteria from otter clam Lutraria philippinarum. Thai J Vet Med. 2014;1:345-353.   DOI
39 Kurtzman CP, Robnett CJ. Identification of clinically important ascomycetous yeasts based on nucleotide divergence in the 5' end of the large-subunit (26S) ribosomal DNA gene. J Clin Microbiol. 1997;35(5):1216-1223.   DOI
40 Kumar S, Stecher G, Li M, et al. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol. 2018;35(6):1547-1549.   DOI
41 Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution. 1985;39(4):783-791.   DOI
42 Houbraken J, Kocsube S, Visagie CM, et al. Classification of Aspergillus, Penicillium, Talaromyces and related genera (Eurotiales): an overview of families, genera, subgenera, sections, series and species. Stud Mycol. 2020;95:5-169.   DOI
43 Sandhu DK, Sandhu RS. A new species of Penicillium isolated from sputum. Can J Bot. 1963;41(8):1273-1274.   DOI
44 Agurell SL. Costaclavine from Penicillium chermesinum. Experientia. 1964;20(1):25-26.   DOI