International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Microsatellite Analysis of Silkworm Strains (Bombyx mori) of Japan Origin Preserved in Korea

  • Kim, Kee Young (Department of Agricultural Biology, The National Academy of Agricultural Science) ;
  • Kang, Pil Don (Department of Agricultural Biology, The National Academy of Agricultural Science) ;
  • Kim, Mi Ja (Department of Agricultural Biology, The National Academy of Agricultural Science) ;
  • Ryu, Kang Sun (Department of Agricultural Biology, The National Academy of Agricultural Science) ;
  • Park, Jeong Sun (College of Agriculture & Life Sciences, Chonnam National University) ;
  • Kim, Iksoo (College of Agriculture & Life Sciences, Chonnam National University)
  • Received : 2014.05.07
  • Accepted : 2014.05.31
  • Published : 2014.06.30
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Abstract

In order to understand the diversity and genetic relationships of silkworm strains preserved in Korea, we genotyped 78 Bombyx mori strains (Bombycidae: Lepidoptera) originating from Japan, using eight polymorphic microsatellite loci. We obtained per-locus allele numbers ranging from 5 to 16 (with an average value of 9.1), per-locus observed heterozygosity ranging from 0.13 to 1.00, and per-locus polymorphic information content ranging from 0.36 to 0.77, indicating that some loci are highly variable. Phylogenetic analysis with the eight concatenated microsatellite loci showed no clustering based on known strain characteristics and origin. Nineteen strain-specific apomorphic alleles, which discriminated 16 of the 78 silkworm strains, were obtained from eight loci. These strain-specific alleles can thus be utilized for routine discrimination of strains from Japan, without any further typing of other loci. Homozygotes were also observed at some loci (27 of 118 genotypes), which can also be used to discriminate several strains by typing a few loci. These results showed that eight microsatellite loci described herein were sufficiently variable to discriminate among the 78 silkworm strains we examined, and may be useful for future investigations of this economically important species.

Keywords

References

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