• Title/Summary/Keyword: Sequence characteristics

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Characterization of Cucumber Mosaic Virus Isolated from Passion Fruit (Passiflora edulis) in Korea (국내 패션프루트(Passiflora edulis)에서 분리한 Cucumber Mosaic Virus의 특성)

  • Ye-Yeong Kim;Tae-Seon Park;Ji-Soo Park;Dong-Joo Min;You-Seop Shin;Jin-Sung Hong
    • Research in Plant Disease
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    • v.30 no.1
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    • pp.60-65
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    • 2024
  • In July 2020, total RNA was extracted from passion fruit (Passiflora edulis) leaves showing virus symptoms such as chlorotic spots and vein banding in Haenam, South Korea. Cucumber mosaic virus (CMV)-HN2 was identified through reverse transcription polymerase chain reaction and sequencing analysis. To confirm the biological characteristics of the CMV infecting passion fruit, 10 indicator plants were inoculated with CMV-HN2, and the results showed a typical CMV symptoms. Phylogenetic analysis based on the amino acid of the coat protein (CP) of CMVs revealed that the CMV passion fruit isolates belonged to subgroup I, among which CMV-HN2 belonged to subgroup IA. Additionally, CMVs isolated from passion fruit in Korea have amino acid sequence variation between the subgroup. Among them, CMV-HN2 had four to eight amino acid differences in CP from other CMV isolates from passion fruit. These results confirm the presence of genetic diversity in the CPs of passion fruit CMV isolates.

The Development and Characteristics of Diluvial Soils on the Catena (홍적층(洪積層) Catena에서 토양(土壤)의 발달(發達)과 특성(特性))

  • Rim, Sang-Kyu;Choi, Jyung
    • Korean Journal of Soil Science and Fertilizer
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    • v.17 no.3
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    • pp.200-206
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    • 1984
  • This study was conducted to examine the morphological, physical and chemical characteristics of the Bancheon, Gopyeong, Hwadong and Deogpyeong series derived from diluvium in Korea. The results were as follows. 1. The sequence of the Bancheon, Gopyeong, Hwadong and Deogpyeong series consists of the soil catena. Thus, the Bancheon and Gopyeong series are weil drained, the Hwadong and Deogpyeong series moderately well drained due to the influence of topography and irrigation water. 2. The surface soils of the Bancheon and Gopyeong series are yellowish red, dark yellowish brown fine silty texture and the subsoils are yellowish red, red and strong brown fine clayey with moderate to strong angular blocky or subangular blocky structure with clayey cutans on the structural face. The surface soils of the Hwadong and Deogpyeong series are dark grayish brown, grayish brown fine silty texture and the subsoils are strong brown, light olive brown and brownish yellow fine silty or fine clayey with moderate to strong prismatic, angular blocky or subangular blocky structure with clayey cutans on the structural face. The consistences of all the subsoil horizons are extremely compact and hard, whereas sticky and plastic when wet. 3. The lower the topography, the higher the silt/clay ratio, soil reaction, organic matter, available water and phosphate content but the higher the topography, the higher the active iron content. 4. These soils are classified as Hapludalfs by Soil Taxonomy in U.S.D.A. 5. These soils must be applied with much lime, phosphate and compost to improve the soil fertility.

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Novel Species Candidates Belonging to the Phyla Bacteroidetes, Firmicutes, and Actinobacteria Isolated from the Halla Mountain Wetlands (제주도 고산 습지에서 분리한 Bacteroidetes, Firmicutes, Actinobacteria 문에 속하는 신종후보 세균)

  • Choi, Ah-Young;Choi, Jae-Hee;Kang, Ji-Young;Choe, Jeong-Uk;Lee, Sang-Hoon;Kim, Ha-Neul;Yi, Ha-Na;Shin, Young-Min;Jahng, Kwang-Yeop;Lee, Hyune-Hwan;Kim, Kyu-Joong;Joh, Ki-Seong;Chun, Jong-Sik;Kim, Seung-Bum;Cho, Jang-Cheon
    • Korean Journal of Environmental Biology
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    • v.29 no.3
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    • pp.126-137
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    • 2011
  • Although Sumunmulbangdui wetland at the Halla Mountain in Jeju Island, a kind of montane wetlands, has been considered to bear high biodiversity, no study has been reported on the bacterial diversity. In this study, soil and water samples were collected from the wetland in order to isolate novel bacterial species. Bacterial strains belonging to the phyla Bacteroidetes, Firmicutes, and Actinobacteria were isolated after spreading soil and water samples onto solid agar media. The 16S rRNA gene sequences of the strains assigned to the three phyla were compared to those of type strains of the species in the phyla. The strains that showed less than 98.7% 16S rRNA gene sequence similarity to the validly published species were considered to be novel species candidates. A total of 32 strains were regarded as novel species candidates in the phyla Bacteroidetes, Firmicutes, and Actinobacteria. Diversity of novel species candidates was very low; the candidates were confined to only few genera. In the Bacteroidetes, 13 novel candidate species were affiliated with the genera Mucilaginibacter, Sphingobacterium, Pedobacter, Flavobacterium, and Chryseobacterium. A total of 13 novel candidate species that assigned to the genera Paenibacillus Lysinibacillus, and Bacillus were identified in the phylum Firmicutes. Only two candidate species that belonged to the genera Mycobacterium and Nocardia were excavated in the Actinobacteria. Cultural, physiological, and chemotaxonomic characteristics have been determined for the novel species candidates, and the characteristics are described in this study.

Isolation of Nontuberculous Mycobacteria by DNA Probe and Clinical Characteristics of Patients with NTM Pulmonary Disease (DNA probe를 이용한 비결핵항산균의 분리 및 폐질환자들의 임상적 특징)

  • Kim, Hee Kyoo;Kim, Yu Ri;Park, Jung Pil;Kim, Nang Hee;Ok, Chul Ho;Jung, Maan Hong;Jang, Tae Won;Jeong, Seok Hoon;Kim, Cheol Min;Park, Hee Kyung
    • Tuberculosis and Respiratory Diseases
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    • v.58 no.3
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    • pp.248-256
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    • 2005
  • Background : Nontuberculous mycobacterial (NTM) infections are increasingly being recognized as a cause of chronic pulmonary disease. This study describes the prevalence of NTM species from clinical specimens and the clinical characteristics of NTM pulmonary disease. Material and Methods : The NTM isolated from March 2003 to December 2003 at the Kosin Medical Center were identified using an oligonucleotide chip containing the internal transcribed space (ITS) sequence. The medical records of the patients with the NTM isolates, who fulfilled the 1997 ATS diagnostic criteria for NTM pulmonary disease, were analyzed, retrospectively. Results : Twenty four species (24.2%) of NTM were isolated from 99 cultured AFB specimens. M. avium complex (MAC) (13 isolates), M. szulgai (3), M. kansasii (2), M. malmoense (2), M. abscessus (1), M. chelonae (1), M. scrofulaceum (1), and unclassified (1). Of the 23 patients with isolated NTM, 11 patients were found to be compatible with a NTM pulmonary infection according to the ATS criteria; MAC was found in 6 cases (54.5%), M. szulgai in 2 cases (18.2%), and M. abscessus, M. szulgai, M. kansasii and M. malmoense in 1 case each (9.1%). Ten patients (91%) were male and the median age at diagnosis was 61 years. In the pre-existing diseases, malignant disease was found in 6 cases including 5 patients with lung cancer, and history of old pulmonary tuberculosis was identified in 4 cases. The radiological patterns showed lung destruction lung in 3 cases, a cavitary mass in 3 cases, a nodular pattern in 2 cases, and reticulonodular, consolidation and a bronchiectasis pattern were in 1 case each. Conclusion : Various types of NTM pulmonary diseases were found in a tertiary hospital at Busan, Korea. The NTM pulmonary diseases were caused by MAC, M. szugai, M. kansasii, M. malmoense, M. abscessus, M. chelonae, and M. scrofulaceum in the order of frequency.

The Optimal Production and Characteristics of an Alginate-degrading Enzyme from Vibrio sp. PKA 1003 (Vibrio sp. PKA 1003의 알긴산 분해 조효소 생산 최적 조건과 조효소의 특성)

  • Kim, Hyun-Jee;Kim, Koth-Bong-Woo-Ri;Kim, Dong-Hyun;SunWoo, Chan;Jung, Seul-A;Jeong, Da-Hyun;Jung, Hee-Ye;Lim, Sung-Mee;Ahn, Dong-Hyun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.3
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    • pp.434-440
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    • 2013
  • This study was conducted to screen the characteristics and alginate-degrading activity of marine bacterium isolated from brown seaweed (Sargassum thunbergii). The results of 16S ribosomal RNA sequence analysis the strain the genus Vibrio and the strain was subsequently named Vibrio sp. PKA 1003. The optimum culture conditions for the growth of Vibrio sp. PKA 1003 were at pH 7, 3% NaCl, $25^{\circ}C$, and 6% alginic acid, with a 48-hour incubation time. A crude enzyme preparation from Vibrio sp. PKA 1003, showed its highest levels of alginate-degradation activity when cultured at pH 9, $30^{\circ}C$, and 6% alginic acid, with a 63-hour incubation time. Thin layer chromatography analyses confirmed that the crude enzyme released monomers or oligomers from sodium alginate, and results from trypsin treatment showed that the alginate degrading activity depends on this enzyme produced by Vibrio sp. PKA 1003. These results suggest that Vibrio sp. PKA 1003 and its alginate-degrading crude enzyme is useful for the production of alginate oligosaccharides.

Craniopharyngioma : Comparison of Tumor Characteristics Relevant with Initial Symptomatology between Children and Adults (두개인두종 : 소아와 성인에서 초기 증상과 연관된 종양의 특징 비교)

  • Park, Dong Hyuk;Park, Jung Yul;Kim, Joo Han;Jung, Yong Gu;Lee, Hoon Kap;Lee, Ki Chan;Suh, Jung Keun
    • Journal of Korean Neurosurgical Society
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    • v.30 no.8
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    • pp.985-991
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    • 2001
  • Objectives : The craniopharyngioma is a benign tumor located at least in part in the suprasellar cistern. However, the symptoms and signs from this tumor may be determined not only by the location of the tumor but also by its size and the age of the patient. The objective of our study is to analyze retrospectively the clinical manifestations of craniopharyngiomas with regards to tumor characteristics in children and adults. Material and Methods: Twenty-three patients(16 adults, 7 children) treated for craniopharyngioma between 1990 and 1999 were studied to demonstrate the relationship of tumor size, growth pattern, and its invasiveness with clinical symptoms. As part of the assessment, 16 adults(M : F=8 : 8, mean age : 43.7 years) and 7 children(M : F=5 : 2, mean age : 10.1 years) underwent magnetic resonance(MR) imaging and computerized tomography(CT) scanning with a three-dimensional volume acquisition sequence. Results : The three major cardinal signs were defined to increased intracranial pressure, endocrine dysfunction, and visual problems. The tumor size in child group was larger than that in adult group. Also, visual problems, symptoms of increased intracranial pressure and hydrocephalus were more frequently observed in child group. However, endocrine dysfunction and neuropsychological symptoms related with hypothalamic connections to the thalamus, pituitary, frontal lobe, and other cortical areas were more frequent in adult group. Conclusions: In our series, the tumor size and invasiveness of craniopharyngioma revealed to be relevent with initial symptoms of increased intracranial pressure and visual symptoms which were more frequent in child group. As for the growth pattern, we did not find major difference between adults and children.

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Spatial Characteristics of Pollutant Concentrations in the Streams of Shihwa Lake (시화호 유입하천의 수질오염물질 농도에 관한 연구)

  • Jang, Jeong-Ik;Han, Ihn-Sup;Kim, Kyung-Tae;Ra, Kong-Tae
    • Journal of Korean Society of Environmental Engineers
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    • v.33 no.4
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    • pp.289-299
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    • 2011
  • We studied the characteristics of pollutant concentrations in 9 streams that flow into Shihwa Lake in order to provide the scientific data for effective implementation of total pollution loads management system (TPLMS) of the Lake. Suspended solid (SS), chemical oxygen demand (COD), dissolved nutrients ($NO_2$, $NO_3$, $NH_4$, $PO_4$ and $SiO_2$), total phosphorus (TP) and total nitrogen (TN) in stream water from industrial complexes, urban and agricultural regions were determined. Pollutant concentrations in December were higher than that in other sampling periods. COD concentration from industrial complex region with average of 12.6 mg/L was 2 times higher those from urban region (6.6 mg/L) and agricultural region (5.9 mg/L). TP concentration from industrial region also showed higher concentration than other regions. TN concentration in stream water was 5.89 mg/L for industrial region, 3.02 mg/L for urban region and 5.27 mg/L for agricultural region, respectively, suggesting inflow of TN due to fertilizer usage in agricultural field. Relative percentage of nitrogen compounds in TN follows the sequence: $NH_4$ (35.1%) > $NO_2$ (20.0%) > DON (22.8%) > PON (8.9%) > $NO_2$ (3.2%). Concentrations of dissolved nutrients, TP and TN in stream water were 3.2~37.2 times higher than that in Shihwa Lake seawater, therefore large amount of pollutants may be directly entered into Shihwa Lake without any treatment. For Gunja stream of industrial region, pollutants at midstream showed relatively higher concentration compared to upstream and downstream. It is necessary to manage the illegal discharging of sewage and waste water. Our results provide valuable informations on the estimation and reduction of total pollutant loads in the process of establishing adequately strategic and implemental plan of Shihwa Lake TPLMS.

Sorption and Ion Exchange Characteristics of Chabazite: Competition of Cs with Other Cations (차바자이트의 흡착 및 이온 교환 특성: Cs 및 다른 양이온과의 경쟁)

  • Baek, Woohyeon;Ha, Suhyeon;Hong, Sumin;Kim, Seonah;Kim, Yeongkyoo
    • Journal of the Mineralogical Society of Korea
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    • v.29 no.2
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    • pp.59-71
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    • 2016
  • To investigate the sorption characteristics of Cs, which is one of the major isotopes of nuclear waste, on natural zeolite chabazite, XRD, EPMA, EC, pH, and ICP analysis were performed to obtain the informations on chemical composition, cation exchange capacity, sorption kinetics and isotherm of chabazite as well as competitive adsorption with other cations ($Li^+$, $Na^+$, $K^+$, $Rb^+$, $Sr^{2+}$). The chabazite used in this experiment has chemical composition of $Ca_{1.15}Na_{0.99}K_{1.20}Mg_{0.01}Ba_{0.16}Al_{4.79}Si_{7.21}O_{24}$ and its Si/Al ratio and cation exchange capacity (CEC) were 1.50 and 238.1 meq/100 g, respectively. Using the adsorption data at different times and concentrations, pseudo-second order and Freundlich isotherm equation were the most adequate ones for kinetic and isotherm models, indicating that there are multi sorption layers with more than two layers, and the sorption capacity was estimated by the derived constant from those equations. We also observed that equivalent molar fractions of Cs exchanged in chabazite were different depending on the ionic species from competitive ion exchange experiment. The selectivity sequence of Cs in chabazite with other cations in solution was in the order of $Na^+$, $Li^+$, $Sr^{2+}$, $K^+$ and $Rb^+$ which seems to be related to the hydrated diameters of those caions. When the exchange equilibrium relationship of Cs with other cations were plotted by Kielland plot, $Sr^{2+}$ showed the highest selectivity followed by $Na^+$, $Li^+$, $K^+$, $Rb^+$ and Cs showed positive values with all cations. Equilibrium constants from Kielland plot, which can explain thermodynamics and reaction kinetics for ionic exchange condition, suggest that chabazite has a higher preference for Cs in pores when it exists with $Sr^{2+}$ in solution, which is supposed to be due to the different hydration diameters of cations. Our rsults show that the high selectivity of Cs on chabazite can be used for the selective exchange of Cs in the water contaminated by radioactive nuclei.

An Investigation on the Environmental Factors of Certified Organic and Non-pesticide Paddy Soils Cultivating Rice at Goseong-Gun (고성지역 유기농산물과 무농약농산물인증 논토양의 환경 조사)

  • Joo, Heui Sig;Cho, Young Son;Chun, Hyun Sik
    • Journal of Life Science
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    • v.24 no.4
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    • pp.403-410
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    • 2014
  • This study was conducted in organic certification soil for the comparison of heavy metals, nutrients, and irrigated water standards to certify a farm. It was carried out in 811 paddy fields of organic rice (Oryza sativa L.) cultivated at Goseong-Gun. The amounts of 8 heavy metals, Cd, Cu, As, Hg, Pb, $Cr^{6+}$, Zn, and Ni were found to be 0.05, 14.5, 1.08, 0.92, 10.7, 1.34, 35.9, and 22.2 mg $kg^{-1}$ in regular sequence in the organic paddy soil, and they were 0.32, 13.6, 1.01, 0.03, 10.4, 0.91, 42.4 and 22.5 mg $kg^{-1}$ in the non-pesticide paddy soil. In comparing organic and non-pesticide paddy soil with respect to the chemical characteristics of the soil, the average pH and the amount of organic matter, available phosphate and available silicate were 5.88 and 27.6 g $kg^{-1}$, 134.5 mg $kg^{-1}$, and 165.3 mg $kg^{-1}$, while they were 5.78 and 32.1 g $kg^{-1}$, 107.7 mg $kg^{-1}$, and 175.2 mg $kg^{-1}$, respectively. The amount of exchangeable cation $K^+$, $Ca^{2+}$, and $Mn^{2+}$ were 0.25, 5.20, and 1.04 cmol+ $kg^{-1}$ in organic paddy soil, while they were 0.38, 5.13, and 1.19 cmol+ $kg^{-1}$ in non-pesticide paddy soil. The pH, DO, BOD, COD and SS conditions of the irrigated water used in the organic paddy soil were found to be 7.23, 8.40, 2.80, 1.86, and 2.58 mg $l^{-1}$ and the condition of irrigated water used in the non-pesticide paddy soil were found to be 7.65, 9.16, 2.25, 4.11, and 4.00 mg $l^{-1}$, respectively. Based on these findings, we suggest that environmentally-friendly certificates in Korea have to unify organic and non-pesticide agro-products in an organic standard in food policy and control because there is no difference between soil and irrigated water standards in the two certifications.

Characteristic of mycelial growth of cauliflower mushroom (Sparassis latifolia) using replacement culture with Trichoderma and rDNA analysis in genealogy of crossbreeding strain (푸른곰팡이 대치배양에 의한 꽃송이버섯 균사 생장 특성 및 계통간 교잡균주의 rDNA 분석)

  • Oh, Deuk-Sil;Kim, Hyun-Suk;Kim, Young;Wi, An-Jin;Yoon, Byung-Sun;Park, Whoa-Shig;Park, Hyeong-Ho;Wang, Seung-Jin
    • Journal of Mushroom
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    • v.12 no.1
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    • pp.41-51
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    • 2014
  • Cauliflower mushroom widely known high concent of ${\beta}$-glucan for farm cultivation invigoration verified characteristics of mycelia growth, genetic diversity, resistance to Trichoderma by replacement culture with Trichoderma and growth characteristics of new variety crossbleeding strain. The result of replacement culture with Trichoderma for verification resistance about Trichoderma, 6951 (T. viride) strain did not show special change after formation of confrontation line and 6952 (T. spp.) strain was showed more formation of spore after formation of confrontation line. But 6426 (T. harzianum) strain found to encroach part of growth area of cauliflower mushroom mycelia. Among 10 kinds cauliflower mushroom strain, JF02-06 strain collected by Gurye, found did not spore of Trichoderma and thought to be resistant to Trichoderma. The result of crossbleeding after selected that mother strain good growth and formation of fruit body, verified good mycelia growth at JF02-47, 49 and 50 strain in Korean pine of wood-chip media. The result of gene sequence about ITS1, 5.8S and ITS4 for analysis of genetic diversity at crossbleeding strain, found high significance to other cauliflower mushroom in registered Genebank. The result of growth characteristic of spore and mycelia of cauliflower mushroom by observation microscope, size of spore showed water drop shape to major axis $6{\mu}m$ and minor axis $5{\mu}m$ and clamp showed 3 types in mycelia. The wide of mycelia was $3{\mu}m$. The characteristic of mycelia of cauliflower mushroom found to grow mycelia in clamp at approximately 50%. The growth speed of mycelia was $0.507{\mu}m/min$ and 2nd mycelia grown similar speed to mother mycelia at parallel with mother mycelia after growth speed at $0.082{\mu}m/min$. The formation of clamp made small clamp for 5 hours after shown transfer of electrolyte in mycelia inside. The septum formation started after 3 hours and then finally completed after 2 hours. In this study, strain of cauliflower mushroom verified resistance of Trichoderma, genetic diversity and characteristic of mycelia growth. Therefore, basic knowledge of cauliflower mushroom will improve and further contribute to development of mushroom industry.