Browse > Article
http://dx.doi.org/10.3746/jkfn.2013.42.3.434

The Optimal Production and Characteristics of an Alginate-degrading Enzyme from Vibrio sp. PKA 1003  

Kim, Hyun-Jee (Dept. of Food Science & Technology/Institute of Food Science, Pukyong National University)
Kim, Koth-Bong-Woo-Ri (Institute of Fisheries Sciences, Pukyong National University)
Kim, Dong-Hyun (Dept. of Food Science & Technology/Institute of Food Science, Pukyong National University)
SunWoo, Chan (Dept. of Food Science & Technology/Institute of Food Science, Pukyong National University)
Jung, Seul-A (Dept. of Food Science & Technology/Institute of Food Science, Pukyong National University)
Jeong, Da-Hyun (Dept. of Food Science & Technology/Institute of Food Science, Pukyong National University)
Jung, Hee-Ye (Dept. of Food Science & Technology/Institute of Food Science, Pukyong National University)
Lim, Sung-Mee (Dept. of Food Nutrition and Science, Tongmyong University)
Ahn, Dong-Hyun (Dept. of Food Science & Technology/Institute of Food Science, Pukyong National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.42, no.3, 2013 , pp. 434-440 More about this Journal
Abstract
This study was conducted to screen the characteristics and alginate-degrading activity of marine bacterium isolated from brown seaweed (Sargassum thunbergii). The results of 16S ribosomal RNA sequence analysis the strain the genus Vibrio and the strain was subsequently named Vibrio sp. PKA 1003. The optimum culture conditions for the growth of Vibrio sp. PKA 1003 were at pH 7, 3% NaCl, $25^{\circ}C$, and 6% alginic acid, with a 48-hour incubation time. A crude enzyme preparation from Vibrio sp. PKA 1003, showed its highest levels of alginate-degradation activity when cultured at pH 9, $30^{\circ}C$, and 6% alginic acid, with a 63-hour incubation time. Thin layer chromatography analyses confirmed that the crude enzyme released monomers or oligomers from sodium alginate, and results from trypsin treatment showed that the alginate degrading activity depends on this enzyme produced by Vibrio sp. PKA 1003. These results suggest that Vibrio sp. PKA 1003 and its alginate-degrading crude enzyme is useful for the production of alginate oligosaccharides.
Keywords
alginate-degrading enzyme; alginate oligosaccharide; Vibrio sp.; marine bacteria;
Citations & Related Records
Times Cited By KSCI : 7  (Citation Analysis)
연도 인용수 순위
1 Joo DS, Lee JS, Park JJ, Cho SY, Kim HK, Lee EH. 1996. Preparation of oligosaccharides from alginic acid by enzymic hydrolysis. Korean J Food Sci Technol 28: 146-151.   과학기술학회마을
2 Joo DS, Lee EH. 1993. Purification of extracellular enzyme produced by Vibrio sp. AL-145. J Korean Soc Food Nutr 22: 234-239.   과학기술학회마을
3 Shin JW. 2012. Cloning and characterization of oligomeric alginate lyase from Stenotrophomonas sp. KJ-2. MS Thesis. Kyung Hee University, Gyeonggi-do, Korea.
4 Lee JH, Lee EY. 2003. Isolation of alginate-degrading marine bacteria and characterization of alginase. J Life Sci 13: 718-722.   과학기술학회마을   DOI   ScienceOn
5 Jung DY, Son CW, Kim SK, Kim YJ, Chung CH, Lee JW. 2009. Biosorption of metal ions by seaweed alginate, polyguluronate and polymannuronate. J Life Sci 19: 553-560.   과학기술학회마을   DOI   ScienceOn
6 Lange III LG, Spilburg CA. 1989. Use of sulfated polysaccharides to decrease cholesterol and fatty acid absorption. US Patent 5,063,210.
7 Choi JH, Kim DW. 1997. Effect of alginic acid-added functional drink (HAEJOMIIN) in brown algae (Undaria pinnatifida) on obesity and biological activity of SD rats. Korean J Life Sci 7: 361-370.   과학기술학회마을
8 Lee BH, Lee SB, Kim WK. 2009. Alginate fiber. Fiber Tech Ind 13: 21-24.
9 Davidson IW, Sutherland IW, Lawson CJ. 1976. Purification and properties of an alginate lyase from a marine bacterium. Biochem J 159: 707-713.   DOI
10 Güven KC, Özsoy Y, Ulutin ON. 1991. Anticoagulant, fibrinolytic and antiaggregant activity of carrageenans and alginic acid. Botanica Marina 34: 429-432.
11 Joo DS, Lee JS, Park JJ, Cho SY, Ahn CB, Lee EH. 1995. Purification and characterization of the intracellular alginase from Vibrio sp. AL-145. Kor J Appl Microbiol Biotechnol 23: 432-438.   과학기술학회마을
12 Kaneko Y, Yonemoto Y, Okayama K, Kimura A, Murata K. 1990. Symbiotic formation of alginate lyase in mixed culture of bacteria isolated from soil. J Ferment Bioeng 69: 192-194.   DOI   ScienceOn
13 Takeuchi T, Murata K, Kusakabe I. 1994. A method for depolymerization of alginate using the enzyme system of Flavobacterium multivolum. Nippon Shokuhin Kogyo Gakkaishi 41: 505-511.   DOI   ScienceOn
14 Joo DS, Lee JS, Cho SY, Shin SJ, Lee EH. 1995. Changes in functional properties of alginic acid by enzymatic degradation. Korean J Food Sci Technol 27: 86-91.   과학기술학회마을
15 Kim OJ, Lee DG, Lee SM, Lee SJ, Do HJ, Park HJ, Kim A, Lee JH, Ha JM. 2010. Isolation and characteristics of alginate-degrading Methylobacterium sp. HJM27. Kor J Microbiol Biotechnol 38: 144-150.   과학기술학회마을
16 Kim OJ. 2011. Isolation and characterization of alginate degrading bacteria separated from brown seaweed. MS Thesis. Silla University, Busan, Korea.
17 Wasikiewicz JM, Yoshii F, Nagasawa N, Wach RA, Mitomo H. 2005. Degradation of chitosan and sodium alginate by gamma radiation, sonochemical and ultraviolet methods. Radiat Phys Chem 73: 287-295.   DOI   ScienceOn
18 Lee DS, Kim HR, Pyeun JH. 1998. Effect of low-molecularization on rheological properties of alginate. J Korean Fish Soc 31: 82-89.   과학기술학회마을
19 Lee JH, Bae MJ, Kim YC, Nam SW. 2009. Identification and characterization of alginate lyase producing Pseudomonas sp. N7151-6. Kor J Microbiol Biotechnol 37: 350-354.   과학기술학회마을
20 Uo MH, Joo DS, Cho SY, Min TS. 2006. Purification and characterization of the extracellular alginase produced by Bacillus licheniformis AL-577. J Korean Soc Food Sci Nutr 35: 231-237.   과학기술학회마을   DOI   ScienceOn
21 Gacesa P. 1987. Alginate modifying enzymes: a proposed unified mechanism of action for the lyases and epimerases. FEBS Lett 212: 199-202.   DOI   ScienceOn
22 Michaud P, Da Costa A, Courtois B, Courtois J. 2003. Polysaccharide lyases: recent developments as biotechnological tools. Crit Rev Biotechnol 23: 233-266.   DOI   ScienceOn
23 Wong TY, Preston LA, Schiller NL. 2000. Alginate lyase: review of major sources and enzyme characteristics, structure- function analysis, biological roles, and application. Annu Rev Microbiol 54: 289-340.   DOI   ScienceOn
24 Hu X, Jiang X, Hwang HM. 2006. Purification and characterization of an alginate lyase from marine bacterium Vibrio sp. mutant strain 510-64. Curr Microbiol 53: 135-140.   DOI   ScienceOn
25 Nisizawa K, Fujibayashi S, Kashiwabara Y. 1968. Alginate lyases in the hepatopancreas of a marine mollusc, Dolabella auricula Solander. J Biochem 64: 25-37.   DOI
26 Kim DE, Lee EY, Kim HS. 2009. Cloning and characterization of alginate lyase from a marine bacterium Streptomyces sp. ALG-5. Mar Biotechnol (NY) 11: 10-16.   DOI   ScienceOn
27 Iwamoto Y, Araki R, Iriyama K, Oda T, Fukuda H, Hayashida S, Muramatsu T. 2001. Purification and characterization of bifunctional alginate lyase from Alteromonas sp. strain no. 272 and its action on saturated oligomeric substrates. Biosci Biotechnol Biochem 65: 133-142.   DOI   ScienceOn
28 Nakada HI, Sweeny PC. 1967. Alginic acid degradation by eliminases from abalone hepatopancreas. J Biol Chem 242: 845-851.
29 Eppley RW, Lasker R. 1959. Alginase in the sea urchin Strongylocentrotus purpuratus. Science 129: 214-215.   DOI   ScienceOn
30 Elyakova LA, Favorov VV. 1974. Isolation and certain properties of alginate lyase VI from the mollusk Littorina sp. Biochimica et Biophysica Acta (BBA)-Enzymology 358: 341-354.   DOI   ScienceOn
31 Muramatsu T, Hirose S, Kayose M. 1977. Isolation and properties of alginate lyase from the mid-gut gland of wreath shell Turbo cornutus. Agric Biol Chem 41: 1939-1946.   DOI
32 Joo DS, Cho SY, Lee EH. 1993. Isolation of alginate-degrading bacteria and production of alginate-degrading activities by the bacteria. Kor J Appl Microbiol Biotechnol 21: 207-213.   과학기술학회마을
33 Eller J, Payne WJ. 1960. Studies on bacterial utilization of uronic acids IV. J Bacteriol 80: 193-199.
34 Nakagawa A, Ozaki T, Chubachi K, Hosoyama T, Okubo T, Iyobe S, Suzuki T. 1998. An effective method for isolating alginate lyase-producing Bacillus sp. ATB-1015 strain and purification and characterization of the lyase. J Appl Microbiol 84: 328-335.   DOI   ScienceOn
35 Li JW, Dong S, Song J, Li CB, Chen XL, Xie BB, Zhang YZ. 2011. Purification and characterization of a bifunctional alginate lyase from Pseudoalteromonas sp. SM0524. Mar Drugs 9: 109-123.   DOI   ScienceOn
36 Kim HS, Bae TJ. 2002. Studies on the hydrolysis of seaweed using microorganisms and its application. J Korean Fish Soc 35: 438-444.   과학기술학회마을   DOI   ScienceOn
37 Stevens RA, Levin RE. 1976. Viscometric assay of bacterial alginase. Appl Environ Microbiol 31: 896-899.
38 Nelson N. 1994. A photometric adaptation of the Somogyi method for the determination of glucose. J Biol Chem 153: 375-380.
39 Baxter RM. 1959. An interpretation of the effects of salts on the lactic dehydrogenase of Halobacterium salinarium. Can J Microbiol 5: 47-57.   DOI   ScienceOn
40 Uo MH, Joo DS, Cho SY. 2006. Screening and cultivation characteristics of alginate degrading bacteria. J Korean Soc Food Sci Nutr 35: 109-114.   과학기술학회마을   DOI   ScienceOn
41 Yonemoto Y, Mutata K, Kimura A, Yamaguchi H, Okayama K. 1991. Bacterial alginate lyase: characterization of alginate lyase-producing bacteria and purification of the enzyme. J Ferment Bioeng 72: 152-157.   DOI   ScienceOn
42 Takeshita S, Sato N, Igarashi M, Muramatsu T. 1993. A highly denaturant-durable alginate lyase from a marine bacterium: purification and properties. Biosci Biotech Biochem 57: 1125-1128.   DOI