식물병연구 (Research in Plant Disease)

한국식물병리학회 (The Korean Society of Plant Pathology)
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국내 패션프루트(Passiflora edulis)에서 분리한 Cucumber Mosaic Virus의 특성

Characterization of Cucumber Mosaic Virus Isolated from Passion Fruit (Passiflora edulis) in Korea

  • 김예영 (강원대학교 농업생명과학대학 식물의학과) ;
  • 박태선 (강원대학교 농업생명과학대학 식물의학과) ;
  • 박지수 (강원대학교 농업생명과학대학 식물의학과) ;
  • 민동주 (강원대학교 농업생명과학대학 식물의학과) ;
  • 신유섭 (강원대학교 농업생명과학대학 식물의학과) ;
  • 홍진성 (강원대학교 농업생명과학대학 식물의학과)
  • Ye-Yeong Kim (Department of Plant Medicine, College of Agriculture and Life Sciences, Kangwon National University) ;
  • Tae-Seon Park (Department of Plant Medicine, College of Agriculture and Life Sciences, Kangwon National University) ;
  • Ji-Soo Park (Department of Plant Medicine, College of Agriculture and Life Sciences, Kangwon National University) ;
  • Dong-Joo Min (Department of Plant Medicine, College of Agriculture and Life Sciences, Kangwon National University) ;
  • You-Seop Shin (Department of Plant Medicine, College of Agriculture and Life Sciences, Kangwon National University) ;
  • Jin-Sung Hong (Department of Plant Medicine, College of Agriculture and Life Sciences, Kangwon National University)
  • 투고 : 2024.01.19
  • 심사 : 2024.02.15
  • 발행 : 2024.03.31
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초록

2020년 7월, 전라남도 해남에서 퇴록병반과 엽맥녹대 등 바이러스 병징이 보이는 패션프루트(Passiflora edulis) 잎으로부터 total RNA를 추출했으며, RT-PCR 검정과 염기서열 결정을 통해 CMV-HN2를 동정하였다. 패션프루트에 감염하는 CMV의 생물학적 특성을 확인하기 위해 10개 지표식물에 CMV-HN2를 접종한 결과, CMV-Fny와 비교하여 전형적인 CMV의 감염 패턴을 나타냈다. CMV 패션프루트 분리주들의 외피단백질에 대한 계통발생학적 분석한 결과, CMV의 패션프루트 분리주들은 subgroup I에 속하는 것으로 나타났으며, 그중 CMV-HN2는 subgroup IA에 속하는 것으로 나타났다. 또한 국내 패션프루트 CMV 분리주들 사이의 subgroup 차이가 확인되었다. 패션프루트 분리주 간의 외피단백질 아미노산 서열을 비교하였을때, CMV-HN2는 다른 분리주들과 특이적으로 4-8개 아미노산 차이가 존재하였다. 이 결과를 통해, 패션프루트 CMV 분리주들의 외피단백질에서 유전적 다양성이 있음을 확인하였다.

In July 2020, total RNA was extracted from passion fruit (Passiflora edulis) leaves showing virus symptoms such as chlorotic spots and vein banding in Haenam, South Korea. Cucumber mosaic virus (CMV)-HN2 was identified through reverse transcription polymerase chain reaction and sequencing analysis. To confirm the biological characteristics of the CMV infecting passion fruit, 10 indicator plants were inoculated with CMV-HN2, and the results showed a typical CMV symptoms. Phylogenetic analysis based on the amino acid of the coat protein (CP) of CMVs revealed that the CMV passion fruit isolates belonged to subgroup I, among which CMV-HN2 belonged to subgroup IA. Additionally, CMVs isolated from passion fruit in Korea have amino acid sequence variation between the subgroup. Among them, CMV-HN2 had four to eight amino acid differences in CP from other CMV isolates from passion fruit. These results confirm the presence of genetic diversity in the CPs of passion fruit CMV isolates.

키워드

과제정보

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (NRF-2021R1F1A1061543).

참고문헌

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