Browse > Article

Isolation of Nontuberculous Mycobacteria by DNA Probe and Clinical Characteristics of Patients with NTM Pulmonary Disease  

Kim, Hee Kyoo (Department of Internal Medicine, Kosin University College of Medicine)
Kim, Yu Ri (Department of Internal Medicine, Kosin University College of Medicine)
Park, Jung Pil (Department of Internal Medicine, Kosin University College of Medicine)
Kim, Nang Hee (Department of Internal Medicine, Kosin University College of Medicine)
Ok, Chul Ho (Department of Internal Medicine, Kosin University College of Medicine)
Jung, Maan Hong (Department of Internal Medicine, Kosin University College of Medicine)
Jang, Tae Won (Department of Internal Medicine, Kosin University College of Medicine)
Jeong, Seok Hoon (Department of Laboratory Medicine, Kosin University College of Medicine)
Kim, Cheol Min (Department of Biochemistry, Pusan National University College of Medicine)
Park, Hee Kyung (Department of Biochemistry, Pusan National University College of Medicine)
Publication Information
Tuberculosis and Respiratory Diseases / v.58, no.3, 2005 , pp. 248-256 More about this Journal
Abstract
Background : Nontuberculous mycobacterial (NTM) infections are increasingly being recognized as a cause of chronic pulmonary disease. This study describes the prevalence of NTM species from clinical specimens and the clinical characteristics of NTM pulmonary disease. Material and Methods : The NTM isolated from March 2003 to December 2003 at the Kosin Medical Center were identified using an oligonucleotide chip containing the internal transcribed space (ITS) sequence. The medical records of the patients with the NTM isolates, who fulfilled the 1997 ATS diagnostic criteria for NTM pulmonary disease, were analyzed, retrospectively. Results : Twenty four species (24.2%) of NTM were isolated from 99 cultured AFB specimens. M. avium complex (MAC) (13 isolates), M. szulgai (3), M. kansasii (2), M. malmoense (2), M. abscessus (1), M. chelonae (1), M. scrofulaceum (1), and unclassified (1). Of the 23 patients with isolated NTM, 11 patients were found to be compatible with a NTM pulmonary infection according to the ATS criteria; MAC was found in 6 cases (54.5%), M. szulgai in 2 cases (18.2%), and M. abscessus, M. szulgai, M. kansasii and M. malmoense in 1 case each (9.1%). Ten patients (91%) were male and the median age at diagnosis was 61 years. In the pre-existing diseases, malignant disease was found in 6 cases including 5 patients with lung cancer, and history of old pulmonary tuberculosis was identified in 4 cases. The radiological patterns showed lung destruction lung in 3 cases, a cavitary mass in 3 cases, a nodular pattern in 2 cases, and reticulonodular, consolidation and a bronchiectasis pattern were in 1 case each. Conclusion : Various types of NTM pulmonary diseases were found in a tertiary hospital at Busan, Korea. The NTM pulmonary diseases were caused by MAC, M. szugai, M. kansasii, M. malmoense, M. abscessus, M. chelonae, and M. scrofulaceum in the order of frequency.
Keywords
Pulmonary tuberculosis; NTM; Microbiology; DNA probe;
Citations & Related Records

Times Cited By SCOPUS : 0
연도 인용수 순위
  • Reference
1 Falkinham JO 3rd. Nontuberculous mycobacteria in the environment. Clin Chest Med 2002;23:529-51   DOI   PUBMED   ScienceOn
2 Lee HW, Kim MN, Shim TS, Bai GH, Pai CH. Nontuberculous mycobacterial pulmonary infection in immunocompetent patients. Tuberc Respir Dis 2002;53:173-82   DOI
3 O'Brien RJ, Geiter LJ, Snider DE Jr. The epidemiology of nontuberculous mycobacterial diseases in the United States: results from a national survey. Am Rev Respir Dis 1987;135:1007-14
4 Embil J, Warren P, Yakrus M, Stark R, Corne S, Forrest D, et al. Pulmonary illness associated with exposure to mycobacterium-avium complex in hot tub water: hypersensitivity pneumonitis or infection? Chest 1997;111:813-6   DOI   ScienceOn
5 France AJ, McLeod DT, Calder MA, Seaton A, My. cobacterium malmoense infections in Scotland: an increasing problem. Thorax 1987;42:593-5   DOI   ScienceOn
6 Gribertz AR, Damsker B, Marchevsky A, Bottone EJ. Nontuberculous mycobacteria in pleural fluid: assessment of clinical significance. Chest 1985;87:495-8   DOI   ScienceOn
7 Lee HW, Cho BW, Kang MS, Kim JS, Lee JW, Nahm CH, et al. Mycolic acid analysis for identification of mycobacterium spp. using high-performance liquid chromatography. Korean J Clin Pathol 1998; 18:77-84
8 Dylewski JS, Zackon HM, Latour AH, Berry GR. Mycobacterium szulgai: an unusual pathogen. Rev Infect Dis 1987;9:578-80   DOI
9 Tenover FC, Crawford JT, Huebner RE, Geiter LJ, Horsburgh CR Jr, Good RC. The resurgence of tu. berculosis: is your laboratory ready? J Clin Microbiol 1993;31:767-70
10 Shin DH. New diagnostic methods in identification of nontuberculous mycobacteria. Korean J Med 2003; 65:1-3
11 Wolinsky E. Nontuberculous mycobacteria and ass. ociated diseases. Am Rev Respir Dis 1979;119:107-59
12 Runyon EH. Anonymous mycobacteria in pulmonary disease. Med Clin North Am 1959;43:273-90   DOI
13 Koh WJ, Kwon OJ, Kang EH, Jeon IS, Pyun YJ, Ham HS, et al. Clinical and radiographic charac. teristics of 12 patients with mycobacterium abscessus pulmonary disease. Tuberc Respir Dis 2003;54:45- 56   DOI
14 Daley CL, Griffith DE. Pulmonary disease caused by rapidly growing mycobacteria. Clin Chest Med 2002;23:623-32   DOI   ScienceOn
15 Park HK, Jang HJ, Song ES, Chang CH, Lee MK, Jeong SH, et al. Detection and genotyping of my. cobacterium species from clinical isolateds and spe. cimens by oligonucleotide array. J Clin Microbiol 2005. (In press)
16 Koh WJ, Kwon OJ, Ham HS, Shu GY, Chung MP, Kim HJ, et al. Clinical significance of nontuberculous mycobacteria isolated from respiratory specimens. Korean J Med 2003;65:10-21
17 Corbett EL, Blumberg L, Churchyard GJ, Moloi N, Mallory K, Clayton T, et al. Nontuberculous myco. bacteria: defining disease in a prospective cohort of South African miners. Am J Respir Crit Care Med 1999;160:15-21   DOI   ScienceOn
18 American Thoracic Society. Diagnostic Standards and Classification of Tuberculosis in Adults and Children. Am J Respir Crit Care Med 2000;161:1376-95   DOI   ScienceOn
19 Ikeue T, Watanabe S, Sugita T, Horikawa S, Suzuki Y, Nishiyama H, et al. Pulmonary infection caused by Mycobacterium szulgai: a case report. Kekkaku 2002;77:427-31
20 Catanzaro A. Diagnosis, differentiating colonization, infection, and disease. Clin Chest Med 2002;23:599- 601   DOI   PUBMED   ScienceOn
21 Griffith DE, Girard WM, Wallace RJ Jr. Clinical features of pulmonary disease caused by rapidly growing mycobacteria: an analysis of 154 patients. Am Rev Respir Dis 1993;147:1271-8   DOI   ScienceOn
22 British Thoracic Society. Management of opportunistic mycobacterial infections: Joint Tuberculosis Committee guidelines 1999. Thorax 2000;55:210-8   DOI   PUBMED   ScienceOn
23 Scientific Committee in Korean Academy of Tuber. culosis and Respiratory Disease. National survey of mycobacterial diseases other than tuberculosis in Korea. Tuberc Respir Dis 1995;42:277-94   DOI
24 Lew WJ. 비결핵항산균증의 임상상 및 국내현황. Tuberc Respir Dis 2002;53(Suppl 2):83-7
25 Evans SA, Colville A, Evans AJ, Crisp AJ, Johnston ID. Pulmonary Mycobacterium kansasii infection: comparison of the clinical features, treatment and outcome with pulmonary tuberculosis. Thorax 1996; 51:1248-52   DOI   ScienceOn
26 Kim EK, Sim TS, Im CM, Lee SD, Go Y, Kim WS, et al. Clinical manifestations of pulmonary infection due to rapidly growing nontuberculous mycobacteria. Tuberc Respir Dis 2003;54:283-94   DOI
27 American Thoracic Society. Diagnosis and treatment of disease caused by nontuberculosis mycobacteria. Am Rev Respir Dis 1990;142:940-53   DOI   ScienceOn
28 American Thoracic Society. Diagnosis and treatment of disease caused by nontuberculous mycobacteria. Am J Respir Crit Care Med 1997;156:S1-25   DOI   ScienceOn
29 Lee H, Park HJ, Cho SN, Bai GH, Kim SJ. Species identification of mycobacteria by PCR-restriction frag .ment length polymorphism of the rpoB gene. J Clin Microbiol 2000;38:2966-71
30 Koh WJ, Kwon OJ, Kang EH, Jeon IS, Pyun YJ, Ham HS, et al. Clinical characteristics of the patients with mycobacterium avium complex pulmonary disease. Tuberc Respir Dis 2003;54:33-44   DOI