• Title/Summary/Keyword: Sequence Maintenance

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A plasmid vector faciliting gene expression in both yeast and mammalian cells

  • Lee, Tae-Ho
    • Journal of Microbiology
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    • v.35 no.2
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    • pp.149-151
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    • 1997
  • A plasmid vector with combined features of yeast shuttle vector and mammalian expression vector was constructed to facilitate expression of cloned gene in both cell-types. All necessary elements required for plasmid maintenance and selection in E. coli, yeast and mammalian cells were size-economically arranged in this plasmid. The numan cytomegalovirus (CMV) immediate early promoter and yeast GAL1 promoter were sequentially placed in front of the gene to be expressed. The synthetic splicing donor and acceptor sequences were inserted into the immediate upstream and downstream of the GAL1 promotor, allowing the CMV promotor to direct the expression of a given gene in mammalian cell environment by splicing out the interfering GAL1 promotor sequence. When the resulting vector containing LacZ as a gene was introduced into yeast and mammalian cells, both cells efficiently produced .betha.-galactosidase, dimonstrating its dual host usage.

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Keyword Analysis Based Document Compression System

  • Cao, Kerang;Lee, Jongwon;Jung, Hoekyung
    • Journal of information and communication convergence engineering
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    • v.16 no.1
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    • pp.48-51
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    • 2018
  • The traditional documents analysis was centered on words based system was implemented using a morpheme analyzer. These traditional systems can classify used words in the document but, cannot help to user's document understanding or analysis. In this problem solved, System needs extract for most valuable paragraphs what can help to user understanding documents. In this paper, we propose system extracts paragraphs of normalized XML document. User insert to system what filename when wants for analyze XML document. Then, system is search for keyword of the document. And system shows results searched keyword. When user choice and inserts keyword for user wants then, extracting for paragraph including keyword. After extracting paragraph, system operating maintenance paragraph sequence and check duplication. If exist duplication then, system deletes paragraph of duplication. And system informs result to user what counting each keyword frequency and weight to user, sorted paragraphs.

Development of Composite Pedestrian Bridge Deck and Its Snap-fit Connection (착탈결구식 복합소재 바닥판의 연결부 거동분석)

  • Lee, Sung-Woo;Jeong, Gyu-Sang
    • Proceedings of the Korean Society For Composite Materials Conference
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    • 2005.11a
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    • pp.280-284
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    • 2005
  • Existing construction materials such as concrete and steel have chronic problems; deterioration and corrosion. Owing to its special features of light weight ‘ high durability, anti-corrosion, composite material used in civil infrastructure can not only solve fundamental problems of deterioration and corrosion, but also reduce both construction and maintenance cost significantly. After the fabrication of deck panel with snap-fit connection by pultrusion through composite design according to stacking sequence of composite laminates and structural analysis, performance of decks will be verified and evaluated by structural tests.

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Structural Characteristics of 스냅핏 Type Composite Deck Panel (착탈결구식 연결구조 복합소재 데크의 거동특성 분석)

  • Lee Sung-Woo;Jeong Gyu-Sang;Cho Sung-Hwan;Sim Young-Sik
    • Proceedings of the Computational Structural Engineering Institute Conference
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    • 2006.04a
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    • pp.719-724
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    • 2006
  • Owing to its special features of light weight, high durability, corrosion-resistant, composite material used in civil infrastructure can not only solve fundamental problems of deterioration and corrosion, but also reduce both construction and maintenance cost significantly. Composite deck panels of 스냅핏 type connection previously designed and fabricated have been redesigned herein. The sensitivity of gaps between snap-fits and tip angles was investigated. Stacking sequence of plies was scrutinized in order to facilitate pultrusion process. Deck panels of redesigned configuration due to bending has been analyzed. A comparison between the preliminary and modified deck design has been made.

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A Study for FMEA and Optimization of Failure Diagnosis Sequence Using Probability of Failure Cause (고장원인 확률을 이용한 FMEA와 고장진단 순서의 최적화)

  • Song, Kee-Tae;Kim, Min-Ho;Baek, Young-Gu;Lee, Key-Seo;Kim, Soo-Myong
    • Proceedings of the KSR Conference
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    • 2007.11a
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    • pp.749-757
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    • 2007
  • Recently, with increasing interested in improvement of operational reliability and the systematic maintenance activities, the RCM analysis has been applied and tried to lots of applicable industries. This study covers applying the probability of failure cause to FMEA, and proposes an analytical method for this. Also, the measures of quantitative classification for the result of failure cause probability are addressed. Based on the field data, this thesis presents an identification for causes and characteristics of failure, and reviews them periodically from the above methodologies. As using FMEA applied the probability of failure cause, we in the future can look forward to improvement of efficiency for failure diagnosis & inspection, and reliability.

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A Cost-Effective Dynamic Redundant Bitonic Sorting Network for ATM Switching (ATM 교환을 위한 비용 효율적인 동적 결함내성 bitonic sorting network)

  • Lee, Jae-Dong;Kim, Jae-Hong;Choe, Hong-In
    • The Transactions of the Korea Information Processing Society
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    • v.7 no.4
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    • pp.1073-1081
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    • 2000
  • This paper proposes a new fault-tolerant technique for bitonic sorting networks which can be used for designing ATM switches based on Batcher-Banyan network. The main goal in this paper is to design a cost-effective fault-tolerant bitonic sorting network. In order to recover a fault, additional comparison elements and additional links are used. A Dynamic Redundant Bitonic Sorting (DRBS) network is based on the Dynamic Redundant network and can be constructed with several different variations. The proposed fault-tolerant sorting network offers high fault-tolerance; low time delays; maintenance of cell sequence; simple routing; and regularity and modularity.

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A study on programming and monitor system of the PLC using GRAFCET (GRAFCET을 이용한 프로그램형 제어기의 제어기능 설계 및 모니터링에 관한 연구)

  • Han, Seung-Soo;Choi, Don;Kim, Hyun-Ki;Woo, Kwang-Bang
    • Proceedings of the KIEE Conference
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    • 1987.11a
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    • pp.90-94
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    • 1987
  • A structured user-friendly procedures which enable users to program in GRAFCET form directly from sequence specifications have been developed. Using these procedures, we can program the programmable logic controller (PLC) in sequential control and realize programming, debugging, and real-time monitoring. GRAFCET has many advantages like parallelism expression, so we can expect higher productivity and easier maintenance than boolean language or relay-ladder diagram specification when adapts them to PLC.

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An Optimal Design of Man-On-Board Storage and Retrieval Warehousing System

  • Song, Jin Young;Lee, Kwang-Hee;Hwang, Hark
    • Journal of Korean Institute of Industrial Engineers
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    • v.19 no.4
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    • pp.97-104
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    • 1993
  • This paper deals with the design problem of a man-on-board(MOB) storage and retrieval warehousing system which is suitable for storing items of small size and light weight. It is assumed that the operator carries out a sequence of retrieving(or storing) operations traveling on a specially designed truck. Considering the operating characteristics of the man-on-board system, an optimal design model is developed in which the investment and maintenance costs of the system are minimized over a time horizon satisfying a set of constructional restrictions. The model is formulated as a nonlinear integer program and a search algorithm is proposed to find an optimum solution.

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Development of A Cement Mill Control System (시멘트 분쇄설비의 자동제어 시스템 개발)

  • Ju, Sang-Wook;Hwang, Hu-Mor
    • Proceedings of the KIEE Conference
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    • 1998.07g
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    • pp.2420-2423
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    • 1998
  • We developed a cement mill control system to improve performances of cement mill and conveyance facilities in view of TPM(Total Productive Maintenance). The system replaces the conventional sequence control relay panel and the mimic panel by the PLC(Programmable Logic Control) and the operation station with a monitor, respectively. In addition, the updated A3A CPU is added to the melsec PLC. All networks of the system are operated through ethernet communications. This system as a domestic product is setup at S company and outperforms other foreign products in views of operation efficiency and process stability.

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Performance Enhancement of IEEE 802.15.3 MAC for Simultaneously Operating Piconets

  • Peng, Xue;Peng, Gong;Kim, Duk-Kyung
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.32 no.1A
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    • pp.34-43
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    • 2007
  • In the IEEE 802.15.3 Medium Access Control (MAC) protocol, Simultaneously Operating Piconets (SOPs) are linked by the parent/child (P/C) or parent/neighbor (P/N) configuration, which work on a Time Division Multiple Access (TDMA) basis. This provides interference mitigation but the overall throughput is limited because the SOPs share the channel time exclusively. The protocol is not efficient for SOPs if we focus on the combination of interference mitigation and high throughput maintenance. In this paper Public Channel Time Allocation (Public CTA) is proposed, which is able to greatly reduce the inter-piconet interference (IPI) and achieve greater throughput without much loss of link success probability (LSP) in the SOPs. The simulation results based on the SOPs of Direct Sequence Ultra Wideband (DS-UWB) system demonstrate that the proposed scheme effectively supports the coexistence of SOPs, and it can not only significantly improve the overall throughput of SOPs but also maintain high LSP.