• Title/Summary/Keyword: Semi-quantitative

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Transcriptome Analysis of the Barley-Rhynchosporium secalis Interaction

  • Al-Daoude, Antonious;Shoaib, Amina;Al-Shehadah, Eyad;Jawhar, Mohammad;Arabi, Mohammad Imad Eddin
    • The Plant Pathology Journal
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    • v.30 no.4
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    • pp.425-431
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    • 2014
  • Leaf scald caused by the infection of Rhynchosporium secalis, is a worldwide crop disease resulting in significant loss of barley yield. In this study, a systematic sequencing of expressed sequence tags (ESTs) was chosen to obtain a global picture of the assembly of genes involved in pathogenesis. To identify a large number of plant ESTs, which are induced at different time points, an amplified fragment length polymorphism (AFLP) display of complementary DNA (cDNA) was utilized. Transcriptional changes of 140 ESTs were observed, of which 19 have no previously described function. Functional annotation of the transcripts revealed a variety of infection-induced host genes encoding classical pathogenesis-related (PR) or genes that play a role in the signal transduction pathway. The expression analyses by a semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) revealed that Rar1 and Rpg4 are defense inducible genes, and were consistent with the cDNA-AFLP data in their expression patterns. Hence, the here presented transcriptomic approach provides novel global catalogue of genes not currently represented in the EST databases.

Oleuropein Induces Apoptosis Via the p53 Pathway in Breast Cancer Cells

  • Hassan, Zeinab Korany;Elamin, Maha Hussein;Omer, Sawsan Ali;Daghestani, Maha Hassan;Al-Olayan, Ebtesam Salah;Elobeid, Mai AbdelRahman;Virk, Promy
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.11
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    • pp.6739-6742
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    • 2013
  • Background: Breast cancer is a major health problem worldwide. Olive oil induces apoptosis in some cancer cells due to phenolic compounds like oleuropein. Although oleuropein has anticancer activity, the underlying mechanisms of action remain unknown. The study aimed to assess the mechanism of oleuropin-induced breast cancer cell apoptosis. Materials and Methods: p53, Bcl-2 and Bax gene expression was evaluated by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) in luminal MCF-7 cells. Results: Oleuropein-induced apoptosis was accompanied by up-regulation of both p53 and Bax gene expression levels and down-regulation in Bcl2. Conclusions: Oleuropein induces apoptosis in breast tumour cells via a p53-dependent pathway mediated by Bax and Bcl2 genes. Therefore, oleuropein may have therapeutic potential in breast cancer patients by inducing apoptosis via activation of the p53 pathway.

Effects of Dexamethasone on the Burden of Marine Birnavirus (MABV) in Olive Flounder, Paralichthys olivaceus (Dexamethasone 투여가 넙치(Paralichthys olivaceus)의 marine birnavirus (MABV) 감염강도에 미치는 영향)

  • Kwon, Se Ryun;Nam, Yoon Kwon
    • Korean Journal of Ichthyology
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    • v.19 no.2
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    • pp.88-92
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    • 2007
  • The effect of dexamethasone injection on the burden of marine birnavirus (MABV) in asymptomatically infected olive flounder (Paralichthys olivaceus) fingerlings was investigated. In real time PCR analysis, the threshold cycle (Ct) value of the fish injected with dexamethasone was significantly lower than that of the fish in the PBS-injected and no-handling groups. The higher amplification of the MABV gene in the dexamethasone-injected group than the 2 control groups was confirmed also by semi-quantitative RT-PCR. The results indicate an increase of MABV burden in olive flounder fingerlings after a single injection with dexamethasone.

Optimum actuator placement for damping of vibrations using the Prestress-Accumulation Release control approach

  • Poplawski, Blazej;Mikulowski, Grzegorz;Pisarski, Dominik;Wiszowaty, Rafal;Jankowski, Lukasz
    • Smart Structures and Systems
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    • v.24 no.1
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    • pp.27-35
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    • 2019
  • This paper proposes a quantitative criterion for optimization of actuator placement for the Prestress-Accumulation Release (PAR) strategy of mitigation of vibrations. The PAR strategy is a recently developed semi-active control approach that relies on controlled redistribution of vibration energy into high-order modes, which are high-frequency and thus effectively dissipated by means of the natural mechanisms of material damping. The energy transfer is achieved by a controlled temporary removal of selected structural constraints. This paper considers a short-time decoupling of rotational degrees of freedom in a frame node so that the bending moments temporarily cease to be transferred between the involved beams. We propose and test a quantitative criterion for placement of such actuators. The criterion is based on local modal strain energy that can be released into high-order modes. The numerical time complexity is linear with respect to the number of actuators and potential placements, which facilitates quick analysis in case of large structures.

물리정수법에 의한 형광 X선분석과 만장굴석주의 연대측정

  • 택훈
    • Proceedings of the Speleological Society Conference
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    • 1993.07a
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    • pp.163-172
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    • 1993
  • Cheju island is situated at about 90km south of southern most tip of the Korean peninsula. Its shape is an ellips with size of 80$\times$40$km^2$, and it is characterized by a symmetrical form whose peak is Mt. Halla (1950m). Major chemical composition of the rock samples from Manjang gul cave is determined by XRF using the fundamental parameter method consisting of fully auto quantitative analysis, semi quantitative analysis (order estimation) and group quantitative analysis. Judging from chemical composition ($SiO_2 = 53.07Wt% Fe_2 O_3=11.34Wt%, MgO=6.48Wt%, Na_2 O=3.07Wt%, K_2 O=1.05Wt%$), this rocks may belong to non alkalic basalt. K-Ar ages of two rocks samples from the Manjang gul cave are also determined. The discrepancy of K-Ar ages is found. They are 0.03Ma and 0.42Ma, respectively. This paper describes some problems experienced in dating young volcanic rocks and then discusses chemical composition, X-ray fluorescence analysis and the age of the formation of a lava tunnel such as Manjang gul cave in cheju Island.

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물리정수법에 의한 형광 X선분석과 만장굴석주의 연대측정

  • 택훈
    • Proceedings of the Speleological Society Conference
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    • 1994.11a
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    • pp.108-109
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    • 1994
  • Cheju island is situated at about 90km south of southern most tip of the Korean peninsula. Its shape is an ellips with size of 80$\times$$40km^2$, and it is characterized by a symmetrical form whose peak is Mt. Halla (1950m). Major chemical composition of the rock samples from Manjang gul cave is determined by XRF using the fundamental parameter method consisting of fully auto quantitative analysis, semi quantitative analysis (order estimation) and group quantitative analysis. Judging from chemical composition ($SiO_2=53.07Wt% Fe_2O_3=11.34Wt%, MgO=6.48Wt%, Na_2O=3.07Wt%, K_2O=1.05Wt%$), this rocks may belong to non alkalic basalt. K-Ar ages of two rocks samples from the Manjang gul cave are also determined. The discrepancy of K-Ar ages is found. They are 0.03Ma and 0.42Ma, respectively. This paper describes some problems experienced in dating young volcanic rocks and then discusses chemical composition, X-ray fluorescence analysis and the age of the formation of a lava tunnel such as Manjang gul cave in cheju Island.

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물리정수법에 의한 형과 X 선분석과 만장굴석주의 연대측정

  • ;Hong, Si-Hwan
    • Journal of the Speleological Society of Korea
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    • v.20 no.21
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    • pp.34-43
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    • 1989
  • Cheju island is situated at about 60km south of southern most tip of the Korean peninsula. Its shape is an ellips with size of 80*40$\textrm{km}^2$, and it is characterized by a symmetrical form whose peak is Mt. Halla(1950m). Major chemical composition of the rock samples from Manjang gul cave is determined by XRF using the fundamental parameter method consisting of fully auto quantitative analysis, semi quantitative analysis (order estimation) and group quantitative analysis. Judging from chemical composition (SiO$_2$ = 53.07Wt%, Fe$_2$O$_3$ = 11.34Wt%, MgO=6.48Wt%, Na$_2$O=3.07Wt%, $K_2$O=1.05Wt%), this rocks from the Manjang gul cave are also determined. The discrepancy of K-Ar ages is found. They are 0.03 Ma and 0.42Ma, respectively. This paper describes some problems experienced in dating young volcanic rocks and then discusses chemical composition, X-ray fluorescence analysis and the age of the formation of a lava tunnel such as Manjang gul cave in cheju Island.

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Typical Pseudo-accident Scenarios in the Petrochemical Process (석유화학 공정의 가상사고 시나리오 유형분석)

  • 윤동현;강미진;이영순;김창은
    • Journal of the Korean Society of Safety
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    • v.18 no.3
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    • pp.75-80
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    • 2003
  • This paper presents a set of typical pseudo-accident scenarios related to major equipments in petrochemical plants, which would be useful for performing such quantitative risk analysis techniques as fault tree analysis, event tree analysis, etc. These typical scenarios address what the main hazard of each equipment might be and how the accident might develop from an "initiating event". The proposed set of accident scenarios consists of total thirteen (13) scenarios specific for five (5) major equipments like reactor, distillation column, etc., and has been determined and screened out of one hundred and twenty-five (125) potential accident scenarios that were generated by performing semi-quantitative risk analysis practically for twenty-five (25) petrochemical processes, considering advices from the operation experts. It is assumed that with simple consideration or incorporation of plant-specific conditions only, the proposed accident scenarios could be easily reorganized or adapted for the relevant process with less time and labor by the safety engineers concerned in the petrochemical industries.ndustries.

Characterization of Eco-Design Checklists (에코디자인 체크리스트 특성 분석)

  • Masoudi, Ali;You, Hee-Cheon;Suh, Suk-Hwan
    • Journal of the Korean Society for Precision Engineering
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    • v.29 no.9
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    • pp.964-970
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    • 2012
  • Various eco-design tools have been developed which can be classified into quantitative, semi-quantitative, and qualitative tools. Practitioners are reluctant to utilize quantitative tools in light of their time-demanding nature. Among the qualitative tools, checklists are simple tools that allow a quick and effective evaluation and consideration of environmental impacts over the entire life cycle of a product. A profound and better understanding of eco-design checklists is needed so that practitioners can apply them appropriately to their product development context. Various types of eco-design checklists are analyzed in the present study based on their attributes and classified in a structured way for their efficient utilization in product development contexts.

A Method for Quantitative Measurement of Lateral Flow Immunoassay Using Color Camera (컬러 카메라를 이용한 측면유동 면역 어세이 정량분석 방법)

  • Park, Jongwon
    • Journal of Biomedical Engineering Research
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    • v.35 no.1
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    • pp.1-7
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    • 2014
  • Among semi-quantitative or fully quantitative lateral flow assay readers, an image sensor-based instrument has been widely used because of its simple setup, cheap sensor price, and compact equipment size. For all previous approaches, monochrome CCD or CMOS cameras were used for lateral flow assay imaging in which the overall intensities of all colors were taken into consideration to estimate the analyte content, although the analyte related color information is only limited to a narrow wavelength range. In the present work, we introduced a color CCD camera as a sensor and a color decomposition method to improve the sensitivity of the quantitative biosensor system which utilizes the lateral flow assay successfully. The proposed setup and image processing method were applied to achieve the quantification of imitatively dispensed particles on the surface of a porous membrane first, and the measurement result was then compared with that using a monochrome CCD. The compensation method was proposed in different illumination conditions. Eventually, the color decomposition method was introduced to the commercially available lateral flow immunochromatographic assay for the diagnosis of myocardial infarction. The measurement sensitivity utilizing the color image sensor is significantly improved since the slopes of the linear curve fit are enhanced from 0.0026 to 0.0040 and from 0.0802 to 0.1141 for myoglobin and creatine kinase (CK)-MB detection, respectively.