Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
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Effects of Dexamethasone on the Burden of Marine Birnavirus (MABV) in Olive Flounder, Paralichthys olivaceus

Dexamethasone 투여가 넙치(Paralichthys olivaceus)의 marine birnavirus (MABV) 감염강도에 미치는 영향

  • Kwon, Se Ryun (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Nam, Yoon Kwon (Department of Aquaculture, Pukyong National University)
  • Received : 2007.03.30
  • Accepted : 2007.05.21
  • Published : 2007.06.30
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Abstract

The effect of dexamethasone injection on the burden of marine birnavirus (MABV) in asymptomatically infected olive flounder (Paralichthys olivaceus) fingerlings was investigated. In real time PCR analysis, the threshold cycle (Ct) value of the fish injected with dexamethasone was significantly lower than that of the fish in the PBS-injected and no-handling groups. The higher amplification of the MABV gene in the dexamethasone-injected group than the 2 control groups was confirmed also by semi-quantitative RT-PCR. The results indicate an increase of MABV burden in olive flounder fingerlings after a single injection with dexamethasone.

Marine birnavirus (MABV)에 무증상적으로 감염된 넙치 (Paralichthys olivaceus) 치어에 면역억제제의 일종인 Dexamathasone을 투여하였을 때 MABV의 감염강도에 영향을 미치는가를 조사하였다. Real time PCR 분석결과 dexamethasone을 투여한 그룹이 생리식염수를 주사한 그룹 및 no handling 그룹에 비해 유의적으로 낮은 Ct 값을 나타냈으며, 또한 semi-quantitative RT-PCR 분석결과에 있어서도 dexamethasone을 주사한 그룹이 대조구 그룹들에 비해 MABV 유전자가 유의적으로 높게 증폭되는 것으로 나타났다. 이러한 결과로부터 dexamethasone 투여가 넙치 치어에 감염된 MABV의 복제를 증가시킴을 확인하였다.

Keywords

Acknowledgement

Supported by : Ministry of Maritime Affairs and Fisheries

References

  1. Balm, P.H.M. 1997. Immune-endocrine interactions. In 'Fish Stress and Health in Aquaculture' (Iwama GK, Pickering AD and Schreck CB, Eds.), pp. 195-221. Cambridge Univ. Press, Cambridge
  2. Chou, H.Y., H.J. Li and C.F. Lo. 1994. Pathogenicity of a birnavirus to hard clam (Meretrix lusoria) and effect of temperature stress on its virulence. Fish Pathol., 29 : 171-175 https://doi.org/10.3147/jsfp.29.171
  3. Chou, H.Y., S.J. Chang, H.Y. Lee and Y.C. Chiou. 1998. Preliminary evidence for the effect of heavy metal cations on the susceptibility of hard clam (Meretrix lusoria) to clam birnavirus infection. Fish Pathol., 33 : 213-219 https://doi.org/10.3147/jsfp.33.213
  4. Goulding, N.J. and R.J. Flower. 1997. Glucocorticoids and the immune system. In 'Stress, Stress Hormones and the Immune System' (Buckingham JC, Gillies GE and Cowell AM, Eds.), pp. 337-356. Wiley, New York
  5. Hosono, N., S. Suzuki and R. Kusuda. 1994. Evidence for relatedness of Japanese isolate of birnaviruses from marine fish to IPNV. J. Fish Dis., 17 : 433-437 https://doi.org/10.1111/j.1365-2761.1994.tb00239.x
  6. Hosono, N., S. Suzuki and R. Kusuda. 1996. Genogrouping of birnaviruses isolated from marine fish: a comparison of VP2/NS junction regions on genome segment A. J. Fish Dis., 19 : 295-302 https://doi.org/10.1111/j.1365-2761.1996.tb00707.x
  7. Isshiki, T., T. Nagano and S. Suzuki. 2001. Infectivity of aquabirnavirus strains to various marine fish species. Dis. Aquat. Org., 46 : 109-114 https://doi.org/10.3354/dao046109
  8. Isshiki, T., T. Nagano, K. Kanehira and S. Suzuki. 2004. Distribution of marine birnavirus in cultured marine fish species from Kagawa Prefecture, Japan. J. Fish Dis., 27 : 89-98 https://doi.org/10.1111/j.1365-2761.2003.00518.x
  9. Oh, M.J., W.S. Kim, S.I. Kitamura, H.K. Lee, B.W. Son, T.S. Jung and S.J. Jung. 2006. Change of pathogenicity in olive flounder Paralichthys olivaceus by co-infection of Vibrio harveyi, Edwardsiella tarda and marine birnavirus. Aquaculture, 257 : 156-160 https://doi.org/10.1016/j.aquaculture.2006.03.009
  10. Pakingking Jr. R., R. Takano, T. Nishizawa, K. Mori, Y. Lida, M. Arimoto and K. Muroga. 2003. Experimental coinfection with aquabirnavirus and viral hemorrhageic septicemia virus (VHSV), Edwardsiella tarda or Streptococcus iniae in Japanese flounder Paralichthys olivaceus. Fish Pathol., 38 : 15-21 https://doi.org/10.3147/jsfp.38.15
  11. Reno, P.W. 1999. Infectious pancreatic necrosis and associated aquatic birnaviruses. CAB INTERNATIONAL, Fish Diseases and Disorders, Vol. 3: Viral, Bacterial and Fungal Infections (Woo PTK and Bruno DW, Eds), pp. 1-55
  12. Sorimachi, M. and T. Hara. 1985. Characteristics and pathogenicity of a virus isolated from yellowtail fingerlings showing ascites. Fish Pathol., 19 : 231-238. (in Japanese with English abstract) https://doi.org/10.3147/jsfp.19.231
  13. van Nieuwstadt, A.P., S.G. Dijkstra and O.L.M. Haenen. 2001. Persistence of herpesvirus of eel Herpesvirus anguillae in farmed European eel Anguilla anguilla. Dis. Aquat. Org., 45 : 103-107 https://doi.org/10.3354/dao045103
  14. Zhang, C.X. and S. Suzuki. 2003. Comparison of the RNA polymerase genes of marine birnavirus strains and other birnaviruses. Arch. Virol., 148 : 745-758 https://doi.org/10.1007/s00705-002-0951-y