Browse > Article
http://dx.doi.org/10.7314/APJCP.2013.14.11.6739

Oleuropein Induces Apoptosis Via the p53 Pathway in Breast Cancer Cells  

Hassan, Zeinab Korany (Department of Zoology, College of Science, King Saud University)
Elamin, Maha Hussein (Department of Zoology, College of Science, King Saud University)
Omer, Sawsan Ali (Department of Zoology, College of Science, King Saud University)
Daghestani, Maha Hassan (Department of Zoology, College of Science, King Saud University)
Al-Olayan, Ebtesam Salah (Department of Zoology, College of Science, King Saud University)
Elobeid, Mai AbdelRahman (Department of Zoology, College of Science, King Saud University)
Virk, Promy (Department of Zoology, College of Science, King Saud University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.14, no.11, 2013 , pp. 6739-6742 More about this Journal
Abstract
Background: Breast cancer is a major health problem worldwide. Olive oil induces apoptosis in some cancer cells due to phenolic compounds like oleuropein. Although oleuropein has anticancer activity, the underlying mechanisms of action remain unknown. The study aimed to assess the mechanism of oleuropin-induced breast cancer cell apoptosis. Materials and Methods: p53, Bcl-2 and Bax gene expression was evaluated by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) in luminal MCF-7 cells. Results: Oleuropein-induced apoptosis was accompanied by up-regulation of both p53 and Bax gene expression levels and down-regulation in Bcl2. Conclusions: Oleuropein induces apoptosis in breast tumour cells via a p53-dependent pathway mediated by Bax and Bcl2 genes. Therefore, oleuropein may have therapeutic potential in breast cancer patients by inducing apoptosis via activation of the p53 pathway.
Keywords
Apoptotic pathway; Bax gene; breast cancer; Bcl2 gene; oleuropein; p53 gene; semi-quantitative RT-PCR;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Parzonko A, Czerwinska ME, Kiss AK, Naruszewicz M (2013). Oleuropein and oleacein may restore biological functions of endothelial progenitor cells impaired by angiotensin II via activation of Nrf2/heme oxygenase-1 pathway. Phytomedicine, 20, 1088-94.   DOI   ScienceOn
2 Pasban-Aliabadi H, Esmaeili-Mahani S, Sheibani V, et al (2013). Inhibition of 6-hydroxydopamine-induced PC12 cell apoptosis by olive (Olea europaea L.) leaf extract is performed by its main component oleuropein. Rejuvenation Res, 16, 134-42.   DOI   ScienceOn
3 Saad ED (2011). Endpoints in advanced breast cancer: methodological aspects and clinical implications. Indian J Med Res, 134, 413-8.
4 Santiago-Mora R, Casado-Diaz A, De Castro MD, Quesada-Gomez JM (2011). Oleuropein enhances osteoblastogenesis and inhibits adipogenesis: the effect on differentiation in stem cells derived from bone marrow. Osteoporos Int, 22, 675-84.   DOI   ScienceOn
5 Sirianni R, Chimento A, De Luca A, et al (2010). Oleuropein and hydroxytyrosol inhibit MCF-7 breast cancer cell proliferation interfering with ERK1/2 activation. Mol Nutr Food Res, 54, 833-40.
6 Struckhoff AP, Patel B, Beckman BS (2010). Inhibition of p53 sensitizes MCF-7 cells to ceramide treatment. Int J Oncol 37, 21-30.
7 Taylor WR, Agarwal ML, Agarwal A, Stacey DW, Stark GR (1999). p53 inhibits entry into mitosis when DNA synthesis is blocked. Oncogene, 18, 283-95.   DOI
8 Zare L, Esmaeili-Mahani S, Abbasnejad M, et al (2012). Oleuropein, chief constituent of olive leaf extract, prevents the development of morphine antinociceptive tolerance through inhibition of morphine-induced L-type calcium channel overexpression. Phytother Res, 26, 1731-7.   DOI   ScienceOn
9 Abe R, Beckett J, Nixon A, et al (2011). Olive oil polyphenol oleuropein inhibits smooth muscle cell proliferation. Eur J Vasc Endovasc Surg, 41, 814-20.   DOI   ScienceOn
10 Borresen-Dale AL (2003). TP53 and breast cancer. Hum Mutat, 21, 292-300.   DOI   ScienceOn
11 Campolo M, Di Paola R, Impellizzeri D, et al (2013). Effects of a polyphenol present in olive oil, oleuropein aglycone, in a murine model of intestinal ischemia/reperfusion injury. J Leukoc Biol, 93, 277-87.   DOI
12 Chipuk JE, Kuwana T, Bouchier-Hayes L, et al (2004). Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis. Science, 303, 1010-4.   DOI   ScienceOn
13 Choudhuri T, Pal S, Agwarwal ML, Das T, Sa G (2002). Curcumin induces apoptosis in human breast cancer cells through p53-dependent Bax induction. FEBS Lett, 512, 334-40.   DOI   ScienceOn
14 Das T, Sa G, Sinha P, Ray PK (1999). Induction of cell proliferation and apoptosis: dependence on the dose of the inducer. Biochem Biophys Res Commun, 260, 105-10.   DOI   ScienceOn
15 Elamin MH, Daghestani MH, Omer SA, et al (2013). Olive oil oleuropein has anti-breast cancer properties with higher efficiency on ER-negative cells. Food Chem Toxicol, 53, 310-6.   DOI   ScienceOn
16 Fridman JS, Lowe SW (2003). Control of apoptosis by p53. Oncogene, 22, 9030-40.   DOI   ScienceOn
17 Hassan ZK, Elamin MH, Daghestani MH, et al (2012). Oleuropein induces anti-metastatic effects in breast cancer. Asian Pac J Cancer Prev, 13, 4555-9.   DOI   ScienceOn
18 Hollstein M, Sidransky D, Vogelstein B, Harris CC (1991). p53 mutations in human cancers. Science, 253, 49-53.   DOI
19 Jemal A, Bray F, Center MM, et al (2011). Global cancer statistics. CA Cancer J Clin, 61, 69-90.   DOI
20 Huang KF, Zhang GD, Huang YQ, Diao Y (2012). Wogonin induces apoptosis and down-regulates survivin in human breast cancer MCF-7 cells by modulating PI3K-AKT pathway. Int Immunopharmacol, 12, 334-41.   DOI   ScienceOn
21 Kirkin V, Joos S, Zornig M (2004). The role of Bcl-2 family members in tumorigenesis. Biochim Biophys Acta, 1644, 229-49.   DOI   ScienceOn
22 Kostomoiri M, Fragkouli A, Sagnou M, et al (2013). Oleuropein, an anti-oxidant polyphenol constituent of olive promotes alpha-secretase cleavage of the amyloid precursor protein (AbetaPP). Cell Mol Neurobiol, 33, 147-54.   DOI
23 Lessene G, Czabotar PE, Colman PM (2008). BCL-2 family antagonists for cancer therapy. Nat Rev Drug Discov, 7, 989-1000.   DOI   ScienceOn
24 Li-Weber M (2009). New therapeutic aspects of flavones: the anticancer properties of Scutellaria and its main active constituents Wogonin, Baicalein and Baicalin. Cancer Treat Rev, 35, 57-68.   DOI   ScienceOn
25 Moon DO, Lee KJ, Choi YH, Kim GY (2007). Beta-sitosterol-induced-apoptosis is mediated by the activation of ERK and the downregulation of Akt in MCA-102 murine fibrosarcoma cells. Int Immunopharmacol, 7, 1044-53.   DOI   ScienceOn
26 Oi-Kano Y, Kawada T, Watanabe T, et al (2013). Oleuropein supplementation increases urinary noradrenaline and testicular testosterone levels and decreases plasma corticosterone level in rats fed high-protein diet. J Nutr Biochem, 24, 887-93.   DOI   ScienceOn