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http://dx.doi.org/10.5423/PPJ.NT.04.2014.0033

Transcriptome Analysis of the Barley-Rhynchosporium secalis Interaction  

Al-Daoude, Antonious (Department of Molecular Biology and Biotechnology, AECS)
Shoaib, Amina (Department of Molecular Biology and Biotechnology, AECS)
Al-Shehadah, Eyad (Department of Molecular Biology and Biotechnology, AECS)
Jawhar, Mohammad (Department of Molecular Biology and Biotechnology, AECS)
Arabi, Mohammad Imad Eddin (Department of Molecular Biology and Biotechnology, AECS)
Publication Information
The Plant Pathology Journal / v.30, no.4, 2014 , pp. 425-431 More about this Journal
Abstract
Leaf scald caused by the infection of Rhynchosporium secalis, is a worldwide crop disease resulting in significant loss of barley yield. In this study, a systematic sequencing of expressed sequence tags (ESTs) was chosen to obtain a global picture of the assembly of genes involved in pathogenesis. To identify a large number of plant ESTs, which are induced at different time points, an amplified fragment length polymorphism (AFLP) display of complementary DNA (cDNA) was utilized. Transcriptional changes of 140 ESTs were observed, of which 19 have no previously described function. Functional annotation of the transcripts revealed a variety of infection-induced host genes encoding classical pathogenesis-related (PR) or genes that play a role in the signal transduction pathway. The expression analyses by a semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) revealed that Rar1 and Rpg4 are defense inducible genes, and were consistent with the cDNA-AFLP data in their expression patterns. Hence, the here presented transcriptomic approach provides novel global catalogue of genes not currently represented in the EST databases.
Keywords
barley; interaction; leaf scald; semi-quantitative RT-PCR; transcriptome changes;
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