• Clinical and Experimental Reproductive Medicine
• /
• v.34 no.3
• /
• pp.179-188
• /
• 2007

Objectives: Many neurological diseases are known to be caused by expansion of trinucleotide repeats (TNRs). It is hard to diagnose the alteration of TNRs with single cell level for preimplantation genetic diagnosis (PGD). In this study, we describe methods optimized for PGD of TNRs related diseases such as Huntington's disease (HD), spinocerebellar ataxia 3 (SCA3) and fragile X syndrome (FXS). Methods: We performed the preclinical assays with heterozygous patient's lymphocytes by single cell PCR strategy. Fluorescent semi-nested PCR and fragment analysis using automatic genetic analyzer were applied for HD and SCA 3. Whole genome amplification with multiple displacement amplification (MDA) method and fluorescent PCR were carried out for FXS. Amplification and allele drop-out (ADO) rate were evaluated in each case. Results: The fluorescent semi-nested PCR of single lymphocyte showed 100.0% of amplification and 14.0% of ADO rate in HD, and 94.7% of amplification and 5.6% of ADO rate in SCA3, respectively. We could not detect the PCR product of CGG repeats in FXS using the fluorescent semi-nested PCR alone. After applying the MDA method in FXS, 84.2% of amplification and 31.3% of ADO rate were achieved. Conclusions: Fluorescent semi-nested PCR is a reliable method for PGD of HD and SCA3. The advanced MDA method overcomes the problem of amplification failure in CGG repeats of FXS case. Optimization of methods for single cell analysis could improve the sensitivity and reliability of PGD for complicated single gene disorders of TNRs.

• Journal of the Korean Society of Manufacturing Process Engineers
• /
• v.14 no.2
• /
• pp.38-43
• /
• 2015

Tungsten inert gas (TIG) welding is commonly used in industries that require airtightness, watertightness, oiltightness, and precision. It is a non-consumable welding method that is commonly used for the welding of non-ferrous metals, but it can be used to weld most metals. The methods of TIG welding can be divided into three types. The first, manual welding is done directly on the metal by a welder with a torch. The second, semi-automatic welding, gets help from a material supplying machine, but it is conducted by a welder. Lastly, automated welding is conducted fully by a machine during its process and operation. Depending on the selection of electrode, the amount of heat that is applied to the base material and the electrode rod changes and makes the shape of welded parts different. A direct-current positive electrode was used for this study. Through the change of shielding gas type on a structural steel (SS-400) that is commonly used in industry, the composition and shape changes in welded parts were detected after welding. The heat-affected area, hardness value, and tensile strength were also identified through hardness testing and tensile testing. In this study, it was found that the higher hardness value of the heat-affected is, the weaker the tensile strength becomes.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.24 no.10B
• /
• pp.1986-1994
• /
• 1999

In this paper, a semi-automatic approach is adopted to extract a semantic object from real-world video sequences human-aided segmentation for the first frame and automatic tracking for the remaining frames. The proposed algorithm has a hierarchical structure using watershed algorithm. Each hierarchy consists of 3 basic steps: First, seeds are extracted from the simplified current frame. Second, region growing bv a modified watershed algorithm is performed to get over-segmented regions. Finally, the segmented regions are classified into 3 categories, i.e., inside, outside or uncertain regions according to region probability values, which are acquired by the probability map calculated from an estimated motion-vector field. Then, for the remaining uncertain regions, the above 3 steps are repeated at lower hierarchies with less simplified frames until every region is classified into a certain region. The proposed algorithm provides prospective results in studio-quality sequences such as 'Claire', 'Miss America', 'Akiyo', and 'Mother and daughter'.

• Atmosphere
• /
• v.29 no.4
• /
• pp.501-509
• /
• 2019

Asian Dust is a meteorological phenomenon that sand particles are raised from the arid and semi-arid regions-Taklamakan Desert, Gobi Desert and Inner Mongolia in China-and transported by westerlies and deposited on the surface. Asian dust results in a negative effect on human health as well as environmental, social and economic aspects. For monitoring of Asian Dust, Korea Meteorological Administration operates 29 stations using a continuous ambient particulate monitor. Kim et al. (2016) developed an automatic quality check (AQC) algorithm for objective and systematic quality check of observed PM₁₀ concentration and evaluated AQC with results of a manual quality check (MQC). The results showed the AQC algorithm could detect abnormal observations efficiently but it also presented a large number of false alarms which result from valid error check. To complement the deficiency of AQC and to develop an AQC system which can be applied in real-time, AQC has been modulated. Based on the analysis of instrument status codes, valid error check process was revised and 6 status codes were further considered as normal. Also, time continuity check and spike check were modified so that posterior data was not referred at inspection time. Two-year observed PM₁₀ concentration data and corresponding MQC results were used to evaluate the modulated AQC compared to the original AQC algorithm. The results showed a false alarm ratio decreased from 0.44 to 0.09 and the accuracy and the probability of detection were conserved well in spite of the exclusion of posterior data at inspection time.

• The Journal of Korean Association of Computer Education
• /
• v.11 no.3
• /
• pp.67-80
• /
• 2008

Various document categorization methods have been studied to provide a user with an effective way of browsing a large scale of documents. They do compares set of documents into groups of semantically similar documents automatically. However, the automatic categorization method suffers from low accuracy. This thesis proposes a semi-automatic document categorization method based on the domains of documents. Each documents is belongs to its initial domain. All the documents in each domain are recursively clustered in a level-wise manner, so that the category tree of the documents can be founded. To find the clusters of documents, the stop-word of each document is removed on the document frequency of a word in the domain. For each cluster, its cluster keywords are extracted based on the common keywords among the documents, and are used as the category of the domain. Recursively, each cluster is regarded as a specified domain and the same procedure is repeated until it is terminated by a user. In each level of clustering, a user can adjust any incorrectly clustered documents to improve the accuracy of the document categorization.

• The Journal of the Korea Contents Association
• /
• v.13 no.1
• /
• pp.1-8
• /
• 2013

The accuracy of a real facial recognition system can be varied according to the accuracy of the eye detection algorithm when we design and implement a semi-automatic facial recognition algorithm depending on the eye position of a database. In this paper, a fully automatic facial recognition algorithm is proposed such that Gabor and LBP features are extracted from fiducial points of a face graph which was created by using fiducial points based on the eyes, nose, mouth and border lines of a face, fitted on the face image. In this algorithm, the recognition performance could be increased because a face graph can be fitted on a face image automatically and fiducial points based LPB features are implemented with the basic Gabor features. The simulation results show that the proposed algorithm can be used in real-time recognition for more than 1,000 faces and produce good recognition performance for each data set.

• Journal of environmental and Sanitary engineering
• /
• v.6 no.2
• /
• pp.33-47
• /
• 1991

1. Water supply treatment plants personnel could not test the water quality control, because most of them rely on the provincial health laboratories about water quality rely rant test. However, in future, plants have to been provided the facilities and equipment of water quality laboratory. 2. Chlorination methods are 89.5% of liquid chlorine and 10.5% of solid chlorine, and the cost per 1 $\m^{3}$ of chlorination is about 1.30 won which chlorination cost is very cheap as 1/142 of drinking water production cost. Input method of chlorine is 35% of automatic method, 56% of semi-automatic, and 9% of other methods, and this is no problem 3. Residual effects of chlorination, in the case of distilled water as a standardized material and 0.2 ppm of seperated residual chlorine, were continued 32 hours in $0^{\circ}C$, and 25 hour in $20^{\circ}C$, of water temperature and in the case of 0.4 ppm of seperated residual chlorine were continued 47 hours in $0^{\circ}C$ and 23 hours in $20^{\circ}C$. 4. In the case of 4 ppm of seperated residual chlorine, residual effects were continued 23 hours in $5^{\circ}C$, 90 hours in $10^{\circ}C$, 78 hours in $15^{\circ}C$, and 60 hours in $20^{\circ}C$ : by the temper; lure of water, continuing residual effects of chlorination are different, so we have to car for the warm season chlorination in the hider temperature. 5. Chlorination effects of drinking waters in the case of 0.4 ppm of seperated residual chlorine were continued 237 hours in $22^{\circ}C$ water : and in the case of rechlorination as 4 ppm of residual chlorine, continued 71 hours in $22^{\circ}C$ water.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
• /
• v.28 no.4
• /
• pp.463-469
• /
• 2010

Since MOLA(Mars Orbiter Laser Altimeter) data, which provides altimetry data for Mars, does not cover the whole Mars area, image matching with MOC imagery should be implemented for the generation of DEM. However, automatic image matching is difficult because of insufficient features and low contrast. In this paper, we present the area based semi-automatic image matching algorithm with MOC-NA(Mars Orbiter Camera ? Narrow Angle) imagery. To accomplish this, seed points describing conjugate points are manually added for the stereo imagery, and interesting points are automatically produced by using such seed points. Produced interesting points being used as initial conjugate points, area based image matching is implemented. For the points which fail to match, the locations of initial conjugate points are recalculated by using matched six points and image matching process is re-implemented. The quality assessment by reversing the role of target and search image shows 97.5 % of points were laid within one pixel absolute difference.

• Journal of The Korea Institute of Healthcare Architecture
• /
• v.26 no.1
• /
• pp.73-84
• /
• 2020

Purpose: Though Korean healthcare services have been upgraded, infection and fire had been broken out in general hospitals. And higher concerns about quality assessment made it to clinical laboratory design guideline studies. So, this study investigates the facilities, equipment and personnel of laboratory medicine focusing on more than five hundred fifty bed hospital, and contributes to make guidelines for safety and efficiency in lab. Methods: Questionnaires to supervisor technologist and field surveys to medical laboratories in korean hospitals have been conducted for the data collection. 16 answers have been analysed statistically by MS Excel program. Results: Most of the sample tests such as hematology, clinical chemistry, immunology, transfusion, urinalysis, microbiology and molecular diagnosis are performed by more than 80% in large sized general hospital laboratory. In the test methods, automatic analyzers are used up to 80%, total laboratory automation up to 43% in clinical chemistry and immunology, and manual tests in all sorts of the test. There are placed in single lab or two and three labs above the ground, which are all in semi-open lab. There is some correlation with the number of specimens and the number of lab people depending on the number of hospital beds. Laboratory environment shows that work distance is good, but evacuation path width, visibility, separation of staff area from automatic analyzer, and equipment installations are needed to have more spaces and gears. Most of the infection controls are equipped with mechanical ventilation, air-conditioning, washbasin and wastewater separation, BSC installation and negative pressure lab room. Implications: Although the laboratory space area is calculated considering the number of hospital beds, type of tests and number of staff, hospital's expertise and the samples numbers per year should be taken into account in the planning of the hospital.

• Journal of the Korea Institute of Information Security & Cryptology
• /
• v.17 no.2
• /
• pp.51-59
• /
• 2007

Software testing is the process of analyzing a software item to detect the differences between existing and required conditions and to evaluate the features of the software item. A traditional testing focuses on proper functionality, not security testing. Fuzzing is a one of many software testing techniques and security testing. Fuzzing methodology has advantage that low-cost, efficiency and so on. But fuzzing has defects such as intervening experts. Also, if there is no specification, fuzzing is impossible. ROAD Tool is automated testing tool for RPC(Remote Procedure Call) based protocol and software without specification. Existing tools are semi-automated. Therefore we must modify these tools. In this paper, we design and implement ROAD tool. Also we verify utility in testing results.