• Journal of Animal Science and Technology
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• 제50권6호
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• pp.775-782
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• 2008

본 연구는 한우의 근내지방도와 연관된 SNP를 규명하고자 Cattle QTLdb와 BcSNPdb를 이용하였다. 먼저 Cattle QTLdb에서 근내지방도에 관련된 QTL 영역을 탐색한 후 BcSNPdb에 적용하여 총 3605개의 SNP를 발굴하였고 이 중에서 347개의 nonsynonymous SNP를 선정하여 PCR 반응이 확인된 160개의 SNP를 확인하였다. 확인된 160개의 SNP를 30차에서 35차 국가후대검정우(n=476)에서 혈연관계가 없으며 근내지방도 성적을 근거로 정규분포상에서 평균±2SD 범위 밖의 성적이 높은 집단 50두와 낮은 집단 50두에 적용한 결과 6개의 근내지방도 관련 SNP(BTS_003888, BTS_012665, BTS_ 018201, BTS_025951, BTS_009454, BTS_052584)를 확인하였다. 확인된 6개의 SNP를 30차에서 35차 국가후대검정우 집단(n=476)에 적용한 결과 BTS_003888, BTS_009454, BTS_052584의 SNP에서 근내지방도와 유의적인 차이를 나타내었고 BTS_025951의 SNP에서는 등지방두께와 유의적인 차이를 보였다(P<0.05). 따라서 본 연구의 결과에서 밝혀진 BTS_003888, BTS_ 009454, BTS_052584, BTS_025951 SNP들은 한우의 근내지방도와 등지방두께의 선발개량모형에 포함하여 진일보하는 보탬의 기틀이기를 기대한다.

• The Korean Journal of Physiology and Pharmacology
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• 제14권6호
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• pp.419-425
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• 2010

Mast cells are activated by specific allergens and also by various nonspecific stimuli, which might induce physical urticaria. This study investigated the functional expression of temperature sensitive transient receptor potential vanilloid (TRPV) subfamily in the human mast cell line (HMC-1) using whole-cell patch clamp techniques. The temperature of perfusate was raised from room temperature (RT, $23{\sim}25^{\circ}C$) to a moderately high temperature (MHT, $37{\sim}39^{\circ}C$) to activate TRPV3/4, a high temperature (HT, $44{\sim}46^{\circ}C$) to activate TRPV1, or a very high temperature (VHT, $53{\sim}55^{\circ}C$) to activate TRPV2. The membrane conductance of HMC-1 was increased by MHT and HT in about 50% (21 of 40) of the tested cells, and the I/V curves showed weak outward rectification. VHT-induced current was 10-fold larger than those induced by MHT and HT. The application of the TRPV 4 activator $3{\alpha}$-phorbol 12,13-didecanoate ($4{\alpha}$ PDD, $1\;{\mu}M$) induced weakly outward rectifying currents similar to those induced by MHT. However, the TRPV3 agonist camphor or TRPV1 agonist capsaicin had no effect. RT-PCR analysis of HMC-1 demonstrated the expression of TRPV4 as well as potent expression of TRPV2. The $[Ca^{2+}]_c$ of HMC-1 cells was also increased by MHT or by $4{\alpha}$ PDD. In summary, our present study indicates that HMC-1 cells express $Ca^{2+}$-permeable TRPV4 channels in addition to the previously reported expression of TRPV2 with a higher threshold of activating temperature.

• Asian Pacific Journal of Cancer Prevention
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• 제14권12호
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• pp.7045-7056
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• 2013

Since the first report of RNA interference (RNAi) less than a decade ago, this type of molecular intervention has been introduced to repress gene expression in vitro and also for in vivo studies in mammals. Understanding the mechanisms of action of synthetic small interfering RNAs (siRNAs) underlies use as therapeutic agents in the areas of cancer and viral infection. Recent studies have also promoted different theories about cell-specific targeting of siRNAs. Design and delivery strategies for successful treatment of human diseases are becomingmore established and relationships between miRNA and RNAi pathways have been revealed as virus-host cell interactions. Although both are well conserved in plants, invertebrates and mammals, there is also variabilityand a more complete understanding of differences will be needed for optimal application. RNA interference (RNAi) is rapid, cheap and selective in complex biological systems and has created new insight sin fields of cancer research, genetic disorders, virology and drug design. Our knowledge about the role of miRNAs and siRNAs pathways in virus-host cell interactions in virus infected cells is incomplete. There are different viral diseases but few antiviral drugs are available. For example, acyclovir for herpes viruses, alpha-interferon for hepatitis C and B viruses and anti-retroviral for HIV are accessible. Also cancer is obviously an important target for siRNA-based therapies, but the main problem in cancer therapy is targeting metastatic cells which spread from the original tumor. There are also other possible reservations and problems that might delay or even hinder siRNA-based therapies for the treatment of certain conditions; however, this remains the most promising approach for a wide range of diseases. Clearly, more studies must be done to allow efficient delivery and better understanding of unwanted side effects of siRNA-based therapies. In this review miRNA and RNAi biology, experimental design, anti-viral and anti-cancer effects are discussed.

• Journal of Ginseng Research
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• 제37권1호
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• pp.135-141
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• 2013

A rapid, sensitive and selective analytical method was developed and validated for the determination of compound K, a major intestinal bacterial metabolite of ginsenosides in human plasma. Liquid-liquid extraction was used for sample preparation and analysis, followed by liquid chromatography tandem spectrometric analysis and an electrospray-ionization interface. Compound K was analyzed on a Phenomenex Luna C18 column ($100{\times}2.00$ mm, 3 ${\mu}m$) with the mobile phase run isocratically with 10 mM ammonium acetate-methanol-acetonitrile (5:47.5:47.5, v/v/v) at a flow rate of 0.5 mL/min. The method was validated for accuracy (relative error <12.63%), precision (coefficient of variation <9.14%), linearity, and recovery. The assay was linear over the entire range of calibration standards i.e., a concentration range of 1 ng/mL to 1,000 ng/mL ($r^2$ >0.9968). The recoveries of compound K after liquid-liquid extraction at 1, 2, 400, and 800 ng/mL were $106.00{\pm}0.08%$, $103.50{\pm}0.19%$, $111.45{\pm}5.21%$, and $89.62{\pm}34.46%$ for intra-day and $85.40{\pm}0.08%$, $94.50{\pm}0.09%$, $112.50{\pm}5.21%$, and $95.87{\pm}34.46%$ for inter-day, respectively. The lower limit of quantification of the analytical method of compound K was 1 ng/mL in human plasma. The developed method was successfully applied to a pharmacokinetic study of compound K after oral administration in ten of healthy human subjects.

• 한방안이비인후피부과학회지
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• 제19권2호
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• pp.1-18
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• 2006

Objective : Although management of asthma has become increasingly effective, its cure remains elusive, necessitating a new modality to prevent or eliminate causes triggering clinical progress. Based in the clinical experiences, a novel decoration Cheongsangbiyeum (CSB), has been developed to treat asthma, which consists of Polyporus, Semen Myristicae, Pericarpium citri Reticulatae, Rhizoma Cimicifugae, Cortex Albizziae, Fructus Rubi, Rhizoma Zedoariae, and Rhizoma Rhei. In the current study, its anti-asthmatic efficacy was evaluated using a mouse model of asthma. Methods : Experimental allergic asthma was induced by repeated intraperitioneal sensitization and intranasal challenge of ovalbumin (OVA). Water extract of CSB (1 mg/mouse/day) was administrated orally whereas control mice on given with identical volume of phosphate-buffered saline (PBS) for 5 days during the course of antigen challenge. When airway hyperreactivity(AHR) measured by ${\bata}-methacoline-induced$ airflow obstruction was compared, AHR of CSB-treated mice was significantly lower than those of control mice, indicating that CM extract can attenuate an asthmatic symptom. Airway recruitment of leukocytes and eosinophils was also markedly reduced by CSB treatment suggesting that oral treatment of CSB can alleviate the airway inflammation. For a better understanding of possible mechanisms underlying anti-asthmatic effet of CSB, cytokine (IL-4, IL-5, IL-13 and $IFN{\gamma}$ levels in bronchoalveola lavage fluid (BALF) and lung tissues were determined. Results : The results showed that cytokine levels were significantly lowered by CSB treatment. Additionally, number of draining lymph node cells was significantly lower than those of control mice. These data indicate that CSB suppress in vivo allergen-specific response. However, notably, levels of type 2 cytokines such as IL-5 and IL-13 were more profoundly influenced. Moreover, in vitro OVA-specific proliferative response and type 2 cytokine (IL-4, IL-5 and IL-13) production lymph node cells was markedly decreased in CSB-treated mice, whereas their $IFN{\gamma}$ production was not significantly altered Thrse data clearly showed a preferential inhibition of type 2 T cell (Th2) response by CSB treatment. This finding was also supported by serum antibody data showing that levels of OVA-specific type 2 antibodies, IgE and IgG1, in CSB-treated mice were significantly lower than in control mice, while type 1 antibody, IgG2a level m rather higher than controls, although the difference was in significant. Conclusions : In conclusion, oral administration of CSB attenuates asthmatic manifestations including AHR ad airway recruitment of eosinophils in a mouse model which possibly results from selective inhibition of Th2 cell response to allergen. Our data suggest a potential clinical application of CSB for control of allergic asthma.

• 한국환경교육학회지:환경교육
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• 제24권4호
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• pp.111-128
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• 2011

This study was aimed at developing a common curriculum for the department of environmental education from 5 colleges of education. The need and background of curriculum reform can be summarized as follow; first, it has been recognized that new national curriculum of 2009 and 2011 created need for training teachers equipped with more integrated competency. Second, global environmental problems such as climate change and energy crisis asked for more responsible choice and action from all citizens. Third, the extremely low hiring rate resulted in the consideration of new working fields for teacher students majoring in environmental education. Fourth, the expansion of new environmental education paradigms including education for sustainable development called for practicing reconstruction of both contends and methods. From a series of research processes including analysis of current curriculum, DACUM, opinion survey and interest groups review, several new approaches for developing new curriculum had been identified as follow; first, content areas of environmental education should be extended beyond environmental natural science. Second, new learning approaches such as project-based learning need to be emphasized for strengthening the identity of environment as a separate subject. Third, more selective majoring system need to be applied in connection with environment government officials, researchers, and social environmental educators. It was recommended that the application of new curriculum developed by the study would be evaluated and managed by teaching conditions surrounding each of the five university members joined this developing processes. However, it needs to be noted that there is not much time because we had experienced zero hiring rate for the last 4 years and environmental policy and education programs are moving rapidly toward sustainable development.

• 대한소아치과학회지
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• 제34권4호
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• pp.685-693
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• 2007

골격성 부조화가 없는 I급 부정교합은 치아-악궁 간의 크기 차이에 의한 총생(crowding)이나 공극(spacing)이 주된 문제점이다. 이와 같은 경미한 공간 부조화를 개선시키고자 할 때, 치열의 종류, 치아-악궁크기 부조화의 양, 환자의 협조도, 또는 환자의 요구 등에 따라 치료 방법은 다양할 수 있다. 본 증례보고에서는 I급 구치부 관계를 지니면서 전치부의 치아-악궁크기 부조화의 양이 크지 않은 영구치열에서 상하악 전치부의 경미한 총생이나 공극이 관찰되는 경우에 사용할 수 있는 clear aligner와 spring aligner 장치의 이용에 관하여 보고하고자 한다. 이와 같은 장치들의 임상 적용에는 몇 가지 제한적인 조건이 있기는 하지만, 잘 선택된 증례에서는 매우 유용하며 편리하게 이용될 수 있다. 특히 총생이 존재하는 경우에는 이를 해소시킬 공간이 치열 내에서 확보되어야 하므로, 확실한 분석과 진단으로 치아 인접면 삭제의 양을 정확히 결정하여 각 치아들의 근심면과 원심면에서의 삭제의 양이 균등히 이루어질 수 있도록 하는 것이 바람직하다. 또한 치간에 공극이 존재하는 경우에는 배열 후 치열궁 둘레의 감소와 함께 상하악 전치부 간의 overjet 문제가 생길 수 있어 대합치열과의 교합관계에 신중을 기하여야 한다.

• 한국과학교육학회지
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• 제37권3호
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• pp.417-427
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• 2017

미디어는 정보 전달 수단으로 사용되는 문자 또는 이미지를 의미하며, 시공간을 넘어 정보 전달을 매개한다. 미디어는 신문과 텔레비전과 같은 올드 미디어부터 인터넷과 스마트폰으로 대표되는 뉴 미디어까지, 테크놀로지의 발달과 함께 점진적 누적적으로 발달해왔다. 미디어 교육의 목표는 미디어의 속성을 이해하고, 미디어에 대한 비판적 해석과 선별적 수용 태도를 기르며, 나아가 미디어를 통해 창의적으로 의미를 표현하고, 의사소통할 수 있는 능력을 기르는 것이다. 연구자는 국어, 사회 교과교육 연구자들과 함께 2016년 7월~12월까지 '미디어 기반 학습에 기초한 한국형 교실수업 모델 개발 연구'를 수행하였다. 본 연구는 해당 과제의 기초 연구로써 연구자는 2006년~2016년 사이 발간된 58편의 논문에서 과학과 미디어 기반 학습과 관련된 문헌 연구를 통해 연구 동향과 변인을 추출하였다. 연구 결과 과학과 미디어 기반 학습 관련 연구가 최근 증가 추세에 있으며, 전체 연구 중 초등학생을 연구 대상으로 삼은 경우가 절반이 넘었다. 조사한 문헌들은 학생 대상 연구, 교사와 예비 교사 관련 연구, 스마트 기기 또는 미디어 콘텐츠 관련 연구, 디지털 교과서 개발 관련 연구로 구분할 수 있었으며, 4개의 변인 중 학생을 대상으로 인지적 정의적 발달 관련 연구와, 미디어 콘텐츠의 개발 및 적용과 관련된 연구가 다수를 이루었다.

• 한국산업보건학회지
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• 제16권3호
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• pp.222-232
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• 2006

Objectives: The objective of this study was to propose the total isocyanate analytical method which involves derivation of 2,4-toluene diisocyanate(2,4-TDI), 2,6-toluene diisocyanate(2,6-TDI), 4,4'-methylenediphenyl diisocyanate(4,4'-MDI) and 1,6-hexamethylene diisocyanate(1,6-HDI) using 2-chlorobenzyl alcohol(2-CBA) or 2,4-dichlorobenzyl alcohol(2,4-DCBA), and analyzing of hydrolysate of the synthesized urethane with the gas chromatography(GC)/flame ionization detector(FID), GC/pulsed discharge ionization detector-electron capture detector(PD-ECD) and GC/mass selective detector(MSD). Methods: Urethanes were synthesized by reacting 2,4-TDI, 2,6-TDI, 4,4'-MDI and 1,6-HDI to 2-CBA or 2,4-DCBA. Urethanes was verified by TLC, HPLC/UVD and GC/MSD. For field application, the most suitable condition that 2-CBA coated in glass fiber filter removed completely and urethanes were not removed was searched. 2-CBA generated from hydrolysis of urethanes according to hydrolysis conditions. Diisocyanates were collected on field air and analyzed. Results: Urethanes which were white and solid phase synthesized by reacting 2,4-TDI, 2,6-TDI, 4,4'-MDI, 1,6-HDI and 2-CBA or 2,4-DCBA. And urethanes were verified by TLC, HPLC/UVD and GC/MSD. The most suitable conditions to remove 2-CBA coated in glass fiber filter were $87^{\circ}C$ and 20 mmHg and urethanes were not removed under same condition. Hydrolysis yields of urethanes were 99 % to 111 %. 2-CBA, the hydrolysate of urethanes was analyzed by GC/FID, GC/PD-ECD and GC/MSD. Conclusions: Simultaneous analysis of 2,4-TDI, 2,6-TDI, 4,4'-MDI and 1,6-HDI deriving with 2-CBA and 2,4-DCBA, along with a total isocyanate analysis, was feasible with GC/FID, GC/PD-ECD and GC/MSD. This result will be a guide of further study on total isocyanate analysis.

• 한국통신학회논문지
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• 제29권12A호
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• pp.1319-1332
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• 2004

최근 MC DS-CDMA시스템은 차세대 고속 데이타 전송을 위해 활발히 연구되고 있지만 높은 PAPR(peak-to-average power ratio)을 가지는 단점이 있다. 반면 CS-CDMA시스템은 코드 선택 방식을 사용함으로써 부 채널수에 상관없이 일정한 크기의 송신 신호로 인해 낮은 PAPR가진다. 본 논문에서는 MC DS-CDMA와 CS-CDMA의 결합한 새로운 다중접속 방식인 MC CS-CDMA(multicarrier code select CDMA)을 제안한다. MC CS-CDMA 시스템은 특별한 경우로서 MC DS-CDMA시스템과 CS-CDMA시스템을 포함한다. MC CS-CDMA 시스템은 주파수 선택적 다중경로 채널과 MRC를 가진 레이크 수신기에서 성능 및 PAPR을 비교 분석한다. 시뮬레이션 결과 MC CS-CDMA 시스템이 CS-CDMA에 비해 높은 PAPR을 가지지만 MC DS-CDMA에 비해 PAPR을 줄일 수 있다. 또한 수치해석의 결과 MC CS-CDMA 시스템이 확산 이득과 시간 다이버시터 이득의 증가로 인해 MC DS-CDMA에 비해 우수한 성능을 가진다. 그러나, MC CS-CDMA 시스템은 수신기의 복잡도 증가와 가입할 수 있는 사용자수가 감소하는 단점을 가진다.