• 제목/요약/키워드: Selaginella tamariscina

검색결과 27건 처리시간 0.036초

수치에 의한 권백의 항산화 효과와 MMP 발현 저해 효과 (Effect of the Processed Selaginella tamariscina on Antioxidation and Inhibition of Matrix Metalloproteinase)

  • 이범천;심관섭;김진희;김진화;표형배
    • 대한화장품학회지
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    • 제32권2호
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    • pp.69-74
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    • 2006
  • 권백은 동양에서 암환자 치료를 위한 전통 약용식물로 알려져 있다. 본 연구에서는 포제기술을 이용한 권백을 항노화 화장품 소재로 적용하고자 다양한 생물학적활성을 평가하였다. 열과 모래를 이용한 포제 권백은 기존 권백을 다른 목적으로 이용하고자 비교하였다. 포제 권백은 페놀함량이 증가하였고 DPPH 라디칼 소거활성도 증가하였다. 세포내 활성산소 소거평가를 위해 사람 섬유아세포를 배양하여 UVB($20 mJ/cm^2$)에 의해 증가된 세포내 활성산소가 포제 권백을 처리함으로써 활성 산소 소거효과가 증가하였다. 사람 섬유아세포에서 UVA에 의해 발현되는 MMP-1효소는 포제 권백에 의해 농도 의존적으로 감소하였다. 결론적으로 포제 권백은 페놀함량의 증가와 항산화 효과가 증가하였고, 자외선에 의한 세포손상을 보호하여 항노화 화장품의 새로운 소재로 이용될 것으로 사료된다.

권백 추출물의 인간 피부 섬유아세포에서의 항산화와 Matrix Metalloproteinase-1 발현저해효과 (Effect of the Selaginella tamariscina Extract on Antioxidation and Inhibition of Matrix Metalloproteinase-1 in Human Skin Fibroblasts)

  • 심관섭;김진화;박성민;이범천;윤여표;표형배
    • 약학회지
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    • 제48권2호
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    • pp.165-170
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    • 2004
  • The production of matrix metalloproteinases (MMPs) by the UV irradiated skin fibroblast and the degradation of extracellular matrix (ECM) by these enzymes is known as one of the main reasons of photoaging. In this paper, to investigate the relationship between aging and Selaginella tamariscina extract (STE), we investigated the effects of antioxidant and expression of UVA-induced MMP-1 in human dermal fibroblasts. STE was found to show scavenging activities of radicals and reactive oxygen species (ROS) with the $IC_{50}$/ values of 65.1 $\mu\textrm{g}$/$m\ell$ against 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical and 40.9 $\mu\textrm{g}$/$m\ell$ against superoxide radicals in the xanthine/xanthine oxidase system, respectively. UVA induced MMP expression was reduced 75.5% by treatment with STE, and MMP-1 mRNA expression was reduced in a dose-dependent manner. Therefore STE was able to significantly inhibition of MMP expression in protein and mRNA level. All these results suggested that STE may act as an anti-aging agent by antioxidation and reducing UVA-induced MMP-1 production.

Antibacterial Effect of Amentoflavone and Its Synergistic Effect with Antibiotics

  • Hwang, Ji Hong;Choi, Hyemin;Woo, Eun-Rhan;Lee, Dong Gun
    • Journal of Microbiology and Biotechnology
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    • 제23권7호
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    • pp.953-958
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    • 2013
  • Selaginella tamariscina is a traditional herb used in medicine. Phytochemical amentoflavone, a biflavonoid class of flavonoids, was isolated from the plant of Selaginella tamariscina. In this study, the antibacterial effects and combination effects of amentoflavone and conventional antibiotics such as ampicillin, cefotaxime, and chloramphenicol were investigated. These results showed that amentoflavone had a considerable antibacterial effect and synergistic interaction with antibiotics against various bacterial strains (fractional inhibitory concentration index ${\leq}$ 0.5), except for Streptococcus mutans. To study the mechanism(s) involved in the synergistic activities between amentoflavone and antibiotics, we detected hydroxyl radical formation using 3'-(p-hydroxyphenyl) fluorescein and measured the $NAD^+/NADH$ ratio by $NAD^+$ cycling assay. The results indicated that the formation of hydroxyl radical would be a cause of the synergistic effect and that this oxidative stress originated from a transient NADH depletion. This study suggests that amentoflavone synergizes with antibiotics and has potential as a therapeutic agent for antimicrobial chemotherapy.

경북의성 멸종위기종 붉은점모시나비 서식지 식물의 생활형 및 식생 (Life form of the Plants and Vegetation in the Habitat of Endangered Parnassius bremeri in Uiseong, Gyeongsangbuk-do)

  • 이남숙;김창환;김도성;최영은;박율진;이경보
    • 한국환경복원기술학회지
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    • 제14권6호
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    • pp.57-69
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    • 2011
  • The present paper deals with a research of life form and vegetation of the plants extant in the habitat of endangered Parnassius bremeri in Uiseong, Gyeongsangbuk-do with a view to restore their habitat and create substitute habitats. The research found that there are a total of 130 vascular plants including 47 families, 96 genera, 113 species, 1 subspecies and 16 varieties. In terms of their life form, hemicryptophytes (H) is the majority of dormancy form with 41 species or 31.54%, while $R_5$ is majority of radicoid form with 72 species or 55.38%, gravitational disseminule form ($D_4$) is majority among the disseminule forms with 68 species or 52.31% and erect type (e) is majority of growth form with 57 species or 43.85%. Major constituents of the habitat are Sedum kamtschaticum, Selaginella tamariscina, Orostachys japonica, Arundinella hirta, Cymbopogon tortilis var. goeringii, Themeda triandra var. japonica, Carex humilis, Indigofera kirilowii, Imperata cylindrica var. koenigii, Festuca ovina, Artemisia capillaris, etc., all of species which are in the initial stage of succession and show simultaneously features characteristic of vegetation on the rock. In the habitat 1. Sedum kamtschaticum- Selaginella tamariscina community and Pinus densiflora community are distributed whereas, in the habitat 2. Sedum kamtschaticum-Selaginella tamariscina community, Sedum kamtschaticum-Carex humilis community, Sedum kamtschaticum-Cymbopogon tortilis var. goeringii community and Sedum kamtschaticum-Imperata cylindrica var. koenigii community are growing and in the habitat 3. Sedum kamtschaticum-Selaginella tamariscina community, Selaginella tamariscina community, Sedum kamtschaticum-Indigofera kirilowii community and Quercus acutissima community are seen distributed there, too. Typical of food plants growing in the habitat is Sedum kamtschaticum, found mostly in the rocky area and its surroundings. Absorption plants are those that usually bloom in the season when imagoes emerge. From the research, it becomes evident that the blooming season of Sedum kamtschaticum, obviously a food plant, corresponds exactly to the emergence timing of imagoes. Cirsium japonicum var. ussuriense, Rosa multiflora, Erigeron annuus, Spiraea prunifolia var. simpliciflora are found to belong to these plants.

Antibacterial and Synergistic Activity of Isocryptomerin Isolated from Selaginella tamariscina

  • Lee, June-Young;Choi, Yun-Jung;Woo, Eun-Rhan;Lee, Dong-Gun
    • Journal of Microbiology and Biotechnology
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    • 제19권2호
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    • pp.204-207
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    • 2009
  • We investigated novel antibacterial and synergistic activities of isocryptomerin isolated from Selaginella tamariscina. Isocryptomerin showed potent antibacterial activity against Gram-positive and Gram-negative bacterial strains including clinical isolates of antibiotic-resistant species such as methicillin-resistant Staphylococcus aureus(MRSA). Additionally, we further investigated the synergistic activity of isocryptomerin with a conventional antibiotic against MRSA. The result indicated that isocryptomerin had considerable synergistic activity in combination with cefotaxime. In summary, the present study suggests that isocryptomerin may have potential as a novel therapeutic agent for treatment of infectious diseases by not only human pathogenic bacteria but also multidrug-resistant bacteria.

Inhibitory Effect of Amentoflavone of Selaginella Tamariscina on MMP-9 Expression through NF-${\kappa}$B and AP-1 in Macrophage Raw 264.7 cells

  • Ahn, Byung-Tae;Shin, Sung-Ahn;Kim, Jun-Gi;Park, Won-Hwan
    • 동의생리병리학회지
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    • 제21권1호
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    • pp.243-249
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    • 2007
  • The French paradox has been attributed to the antioxidant properties of flavonoids present in the red wine. Amentoflavone(AF) is a bi-flavonoid compound with anti-fungal and anti-inflammatory activities. We isolated AF from Selaginella tamariscina, and studied its effects on nuclear factor-B(NF-B)-mediated MMP-9 gene expression in RAW264.7 cells. AF blocked the lipopolysaccharide(LPS)-induced expression of MMP-9. Zymographic and immunoblot analyses showed that AF suppressed LPS-induced MMP-9 expression in a dose-dependent manner. To clarify the mechanistic basis for its inhibition of MMP-9 induction, we examined the effect of AF on the transactivation of MMP-9 gene by luciferase reporter activity using -1.59 kb flanking region. AF potently suppressed the reporter gene activity. This inhibition was characterized by down-regulation of MMP-9, which was transcriptionally regulated at NF-B site and activation protein-1 (AP-1) site in the MMP-9 promoter, two important nuclear transcription factors that are involved in MMP-9 expression. These findings indicate the efficacy of AF in inhibiting MMP-9 expression through the transcription factors NF-B and AP-1 on LPS-induced RAW264.7 cells.

약용식물 잎에서 분리한 국내 미기록 진균 (Unrecorded Fungal Species Isolated from Medicinal Plant Leaves in Korea)

  • 안금란;노형진;이동형;김수산;김준영;김성환
    • 한국균학회지
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    • 제47권4호
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    • pp.319-328
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    • 2019
  • 2018년 6월, 전남 장흥군 약초재배사에 재배중인 부처손(Selaginella tamariscina), 산비장이(Serratula coronata ssp. insularis), 황금(Scutellaria baicalensis), 현삼(Scrophularia buergeriana) 등 4종의 약용식물로부터 병 감염 증후가 보이는 잎을 채집하였다. 채집된 잎으로부터 국내 미기록 진균 4종(Paraboeremia selaginellae, Colletotrichum camelliae, Alternaria eichhorniae, Phomopsis capsica)을 분리 동정하여 이들의 형태학적인 특성과 분자생물학적 특성을 기술하였다.

Antifungal Effect of Amentoflavone derived from Selaginella tamariscina

  • Jung, Hyun-Jun;Sung, Woo-Sang;Yeo, Soo-Hwan;Kim, Hyun-Soo;Lee, In-Seon;Woo, Eun-Rhan;Lee, Dong-Gun
    • Archives of Pharmacal Research
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    • 제29권9호
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    • pp.746-751
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    • 2006
  • Amentoflavone is a plant biflavonoid that was isolated from an ethyl acetate extract of the whole plant of Selaginella tamariscina (Beauv.) spring. 1D and 2D NMR spectroscopy including DEPT, HMQC, and HMBC were used to determine its structure. Amentoflavone exhibited potent antifungal activity against several pathogenic fungal strains but had a very low hemolytic effect on human erythrocytes. In particular, amentoflavone induced the accumulation of intracellular trehalose on C. albicans as a stress response to the drug, and disrupted the dimorphic transition that forms pseudo-hyphae during pathogenesis. In conclusion, amentoflavone has great potential to be a lead compound for the development of antifungal agents.

부처손 잎 추출물의 항산화, 항염 및 피부재생 효능 (Anti-oxidant, Anti-inflammatory, and Wound Healing Activities of Selaginella tamariscina Leaf Extract)

  • 김유진;이지안
    • 융합정보논문지
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    • 제11권4호
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    • pp.194-202
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    • 2021
  • 부처손 잎은 전통한방에서 탈항, 혈뇨, 부정출혈, 월경불순, 자궁출혈 및 만성간염을 치료 목적으로 사용되어 왔다. 본 연구에서는 부처손을 이용하여 얻은 열수추출물(STW)과 80%에탄올추출물(STE)의 항산화, 항염 및 세포 재생활성을 평가하였다. 그 결과 열수추출물과 에탄올추출물의 폴리페놀 화합물 함량은 각각 38.108±0.766 mg/g, 17.927±1.064 mg/g이었다. DPPH와 ABTS radical 소거능 IC50은 열수추출물이 에탄올추출물에 비해 2배 이상 낮았다. MTT 결과, RAW264.7 세포 생존율은 두 추출물 모두 1 mg/mL에서 6% 감소한 반면, HaCaT 세포의 생존율은 50 ㎍/mL에서 18%로 증가하였다. 열수추출물과 에탄올추출물은 LPS로 자극한 RAW264.7 세포의 NO, TNF-�� 그리고 COX-2, PGE2 등의 염증 매개물질의 생성을 억제하였다. 또한 TNF-a에 의한 각질형성 세포의 손상을 저해시켰다. 이상의 결과로 부처손 열수추출물과 에탄올추출물은 피부건강과 미용 식품을 위한 기능성 원료로 가능성이 높다고 사료된다.