• Clinical and Experimental Pediatrics
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• 제45권12호
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• pp.1477-1483
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• 2002

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.179-180
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• 2002

The phospholipase $A_2$($PLA_2$) family represents a diverse group of enzymes that hydrolyze sn-2 fatty acid from the cell membrane. Several mammalian cytosolic $PLA_2$ and secretory $PLA_2$(s$PLA_2$) have been characterized and classified into different families. At present, 12 distinct sPLA$_2$s have been identified in mammals and classified into different groups, depending on their primary structures as characterized by the number and position of cysteine residues. (omitted)

• 대한한의학회지
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• 제23권3호
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• pp.104-118
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• 2002

Objectives : To study the effects of Kumsooyukkun-jeon on asthma. Methods : Asthma was induced to Balb/c mice with ovalbumin using the method of Hatfield et al. We measured the histological profiles of lung and trachea, numbers of cellular compartments in the bronchoalveolar lavage fluid (BALF), numbers and morphology of the mast cells in the trachea, numbers of mucous-secretory cells in the bronchus, morphology of the bronchus, ultramicroscopical appearance of surface of trachea and number of cilia and mucous-secretory cells by scanning electron microscope. Results : 1. Hypertrophy of mucous membrane of trachea and bronchus and bronchioles in the lung, peritracheal, peribronchus and peribronchiolar inflammatory cell infiltration, and mucoid exudate deposition the lumen were observed in control groups but these phenomena were recovered in the Kumsooyukkun-jeon groups. 2. Cellular compartments including neutrophil and eosinophil were increased in the BALF of control groups but these phenomena were recovered in the Kumsooyukkun-jeon groups. 3. Degranulation and decrease of the numbers of mast cells were detected in the trachea of control groups. However, these phenomena were recovered in the Kumsooyukkun-jeon groups. 4. Shedding, decrease of cilia cell and increase of mucous-secretory cells in the surface of the trachea were measured in control groups but these phenomena were recovered in the Kumsooyukkun-jeon groups. Conclusions : It is considered that Kumsooyukkun-jeon has somewhat favorable effects on asthma.

• Applied Microscopy
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• 제10권1_2호
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• pp.77-86
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• 1980

인삼의 근피층에서 확인된 분비관의 분포, 분비상피세포의 미세구조, 후형질성 함유물의 성분등을 광학 및 전자현미경을 이용하여 추구하였다. 1) 분비관은 배축, 근피층, 엽맥 유관속의 배축면과 향축면, 줄기의 유관속을 사이에 두고 피층과 수층 및 수내(髓內)에서 관찰되었다. 2) 근피층에 존재하는 분비관은 형성층에서 분화함이 확인되었고, 분비관의 환상배열층은 근령(根令)을 추정하는 구조적인 지표가 된다고 믿어진다. 3) 분비상피세포는 인(仁), 미토콘드리아, 소포체가 매우 발달되었으며, ribosome, chromoplast, leucoplast, dictyosome과 저장과립인 전분립이 관찰되었다. 4) 상피세포로는 단층이고 세포질과 분비관내에는 sudanophyl, osmophyl inclusion이 확인되었지만 Pyridin 및 alcohol로 lipid를 추출하였을 때에는 이들 함유물이 관찰되지 않았다. 5) Nile blue반응에서 상피세포내에는 oxazin에 반응하는 산성지질이, 분비관에는 oxazone에 반응하는 중성지질이 검출되었다. 6) 세포내 분비과립은 $OsO_4$단일고정시 전자밀도가 매우 높았다. 따라서 이 물질의 주성분은 불포화성지질인것 같으며 인삼의 분비관은 지질대사와 밀접한 관련성이 있는 것으로 믿어지므로 인삼이 지니는 분비관은 지질분비관(lipid canal 또는 lipid duct)으로 명명됨이 바람직하다.

• Applied Microscopy
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• 제32권3호
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• pp.213-222
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• 2002

피조개의 외투막은 바깥 상피층, 결합조직층 그리고 안쪽 상피층으로 구성되어 있다. 상피층은 단층으로 지지세포, 섬모세포 및 분비세포들로 구성되며, 결합조직층은 주로 교원섬유와 근섬유로 이루어져 있다. 안쪽 상피층의 지지세포들은 원주형으로 자유면은 미세융모로 덮여있다. 섬모세포들은 자유면에 섬모와 미세융모를 가지며, 이들 세포의 세포질 상부에서는 다수의 관상 미토콘드리아들이 관찰된다. 분비세포들은 주로 바깥 상피층에서 관찰되며, 분비과립의 형태학적 특징에 따라 A, B, C, D 네 종류로 구분할 수 있다. A형 분비세포들은 점액세포들로서 가장자리와 중앙부의 외투막에서 주로 관찰되며, 이들 세포의 분비과립은 전자밀도가 낮고 막을 가지지 않는다. B형 분비세포들은 발달된 다수의 조면소포체와 골지체 그리고 전자밀도가 높고 막을 가진 분비과립들을 함유한다. C형 분비세포들의 분비과립은 섬유성분의 중심부와 균질성의 주변부로 구분된다. D형 분비세포들은 주로 중앙부와 각정부 외투막의 바깥 상피층에서 관찰되며, 이들 세포의 분비과립은 균질성의 중심부와 과립상의 주변부로 구분된다. 이와 같은 결과들은 피조개 외투막의 바깥 상피층은 패각형성에 관여하며, 안쪽 상피층은 외투강 정화에 관여함을 의미한다.

• Applied Microscopy
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• 제24권1호
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• pp.41-58
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• 1994

The present studies were designed to determine the feasibility of using the rat tracheal epithelium as models for induction of aging. The ultrastructural and cytochemical changes of tracheal epithelium were investigated in rats at ages of five, twelve and twenty four months. Some major changes in the tracheal epithelium with advancing age were observed by electron microscopy. The results were summarized as fellow: 1. With the advance of age, lysosome, vacuole and multivesicular bodies were increased in number and numerous myelinoid bodies were observed in cytoplasm of ciliated cells. 2. In goblet cell, serous cell and brush cell lysosome and myelinoid bodies were increased in number with the advance of age, and an myelinoid bodies was often found within the secretory granule. 3. Cytochemical studies showed that acid phosphatase activities was observed in multivesicular bodies and lysosome, strong activities with the advance of age. And alkaline phosphatase activity are observed in microvilli, granule and lateral membrane of secretory granule cells, and strong activities with age. Consequently suggest that with the advance of age, tracheal epithelium show ultrastructural and cytochemical alteration of some kind of cell organelles in all kind of cell.

• Applied Microscopy
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• 제34권3호
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• pp.199-209
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• 2004

중국얼룩날개모기(Anopheles sinensis; Culicidae; Diptera)를 사육하여 종령 유충의 중장 상피를 구성하는 세포들에 대한 미세구조를 전자현미경으로 관찰하였다. 중장 상피조직은 원주상흡수세포, 재생세포 그리고 분비과립세포 등으로 구성된 단층 점막상피를 이루었다. 원주상흡수세포의 첨단부 원형질막은 미세융모로 변형되어 "brush border"를 형성하였다. 기저원형질막은 심하게 주름이 잡혀 "canaliculi"를 만들며 길게 발달한 사립체와 접해 있었다. 또한 원주상흡수세포는 세포질내에 과립소포체, 사립체 그리고 골지체 등이 고루 발달한 전형적인 물질 흡수세포의 형태이었다. 재생세포는 세포기관이 미분화된 상태이었고, 세포질의 전자밀도가 높아 검게 보였다. 작은 재생세포들은 1개 혹은 $2{\sim}3$개가 집단 (nidi)을 이루어 원주상흡수세포의 기저부 사이에 위치하였다. 직경이 약 $200{\sim}400$ nm 크기의 분비과립을 가지는 분비과립세포가 관찰되었는데 이 세포는 기저막상, 원주상세포의 기부 사이에 위치하여 원추형으로 관찰되었다. 기저막 바깥쪽에는 전형적인 골격근으로 분화된 환상근과 종주근 다발이 있었으며, 기저막과 근육층사이 결합조직인 점막하층에는 신경세포와 기관세지들이 관찰되었다.

• 한국패류학회지
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• 제12권1호
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• pp.1-17
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• 1996

Morphological and histochemical characteristics of the cells in posterior tentacle antenna of Korean slug, Incilaria fruhstorferi were observed with light microscope. The epithelium of the posterior tentacle antenna was composed of supporting cells, sensory neurons and type-a clear cell. The columnar supporting epithelium was widely distributed in the posterior tentacle antenna, and the upper end of the cell was covered with acidic mucopolysaccharide. Nerve endings of the sensory neuron were distributed between type-a clear cells. It was usually located in tentacular knob, and the number of them gradually decrdased as close as tentacular stalk. Several cilia were observed on the nerve ending. Type-a clear cells were very brightly stained with all staining used, and the neutral mucous guanules distributed in the cytoplasm. Collar cells, type-b clear cell and various types of secrdtory cells distributed in the connective tissue. The collar cells were clustering in connective tissue, and the cytoplasm were filled with neutral mucous guanules. The cells and granules were stained with dark brown by silver nitrate stain. Type-b clear cells were irregular in shape and their cytoplasms were brightly stained wth many stains used. Ten types of secretory cells evenly distributed in the connective tissue and muscle layers of the posterior tentacle antenna. The five types of the secretory cells(A, B, E, J and L)seemed to secrete acidic mucopolysaccharide, and the other five type of the cell(C, D, F, H, and L)seemed to secrete neutral mucopolysaccharide. Muscular tissue composed of well-developed thick longitudinal muscle layers and thin circular muscle layers. Type-L secretory cells clustered only in muscular layers and they contained acidic mucopolysaccharides.

• Applied Microscopy
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• 제24권1호
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• pp.1-10
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• 1994

The integumental secretory structure is exocrine unicellular gland located in the epidermis of goldfish, Carassius auratus, and divided into two groups, mucous and granular cells. By the histochemical studies of integumental secretions the mucos cells reacted for acidic polysaccharides, and the granular cells for neutral glycoprotein. According to concentration of the secretion the integumental mucous are gradually sulphated. The mucous cells are typical form of goblet cell located in the upper region of the epidermis, and membrane bounded vesicles of the mucous are observed several size and electron densities by the cellular differentiation. The granular cells in middle and lower epidermis are present syncitial forms occasionally, and contain electron dense granules sized $1.0{\mu}m$ which are accumulated in cytoplasmic process held the cells to the basal lamina. The precursors of the integumental secretory materials are originated from the rough endoplasmic reticulum and next transported through the Golgi apparatus as a form of membrane bounded vesicles. After accomplish this process mature secretions are extruded to integumental surface by the mechanism of merocrine secretion in response to nerve stimulations respectively.

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 2001년도 학술 발표회 진행표 및 논문초록
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• pp.27-27
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• 2001

In exocrine acinar cells, $Ca^{2＋}$ -activated Cl$^{[-10]}$ channels in the apical membrane are essential for fluid secretion, but it is unclear whether such channels are important for Cl$^{[-10]}$ uptake at the base. Whole cell current recording, combined with local uncaging of caged $Ca^{2＋}$, was used to reveal the Cl$^{[-10]}$ channel distribution in mouse pancreatic acinar cells, where ~90％ of the current activated by $Ca^{2＋}$ in response toacetylcholine was carried by Cl$^{[-10]}$ .(omitted)