• Title/Summary/Keyword: SecY

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RNA Helicase activity of SecA protein of Escherichia coli

  • Park, Sukyung;Kim, Hyoungman
    • Proceedings of the Korean Biophysical Society Conference
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    • 1996.07a
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    • pp.24-24
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    • 1996
  • SecA protein of E. coli is essential for the translocation of various precursor proteins across the plasma membrane. Along with it, SecA protein interacts with precursor proteins, SecY/E, SecB and is an ATPase which has multiple ATP binding sites. There is little known about the regulation mechanism of the protein translocation machinery. (omitted)

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Antioxidant effects of selenocysteine on replicative senescence in human adipose-derived mesenchymal stem cells

  • Suh, Nayoung;Lee, Eun-bi
    • BMB Reports
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    • v.50 no.11
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    • pp.572-577
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    • 2017
  • In most clinical applications, human mesenchymal stem cells (hMSCs) are expanded in large scale before their administration. Prolonged culture in vitro results in cellular senescence-associated phenotypes, including accumulation of reactive oxygen species (ROS) and decreased cell viabilities. Profiling of stem cell-related genes during in vitro expansion revealed that numerous canonical pathways were significantly changed. To determine the effect of selenocysteine (Sec), a rare amino acid found in several antioxidant enzymes, on the replicative senescence in hMSCs, we treated senescent hMSCs with Sec. Supplementation of Sec in the culture medium in late-passage hMSCs reduced ROS levels and improved the survival of hMSCs. In addition, a subset of key antioxidant genes and Sec-containing selenoproteins showed increased mRNA levels after Sec treatment. Furthermore, ROS metabolism and inflammation pathways were predicted to be downregulated. Taken together, our results suggest that Sec has antioxidant effects on the replicative senescence of hMSCs.

Analysis of Reverberation Time of Outdoor Space in Apartment Complex According to the Green Wall Conditions (아파트 단지 옥외 공간에서의 녹화 조건에 따른 잔향특성 변화 분석)

  • Cho, Hyun-Min;Shin, Hye-Kyung;Sung, Yo-Han;Kim, Myung-Jun
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2014.10a
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    • pp.874-879
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    • 2014
  • This study is aimed to evaluate the acoustic performances of outdoor apartment complex and to analyze acoustic performances change based on greening conditions. The object space located in Seoul, Yangcheon-gu, Sinjung-dong and classified according to type of space-form. The measurement reverberation time at 500 Hz is 0.86~1.84sec. The simulated reverberation time at 500 Hz is decreased from 1.57sec to 0.57sec in ㅁshape type of 69% treatment with greening and 1.7sec to 0.89sec in ㄷshape type of 70% treatment and 1.05sec to 0.53sec in ㄴshape type of 61.6% treatment.

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A study on the influence of the traverse speed on the mechanical strength of friction stir welding A5083 (A5083 마찰교반접합(F.S.W)시 이송속도가 기계적 강도에 미치는 영향에 관한 연구)

  • 성기완;이준형;오세헌;박근형;윤병수;민택기
    • Proceedings of the KWS Conference
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    • 2001.05a
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    • pp.114-116
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    • 2001
  • This study deals with friction stir welding of aluminum alloy(A5083). Welding conditions were applied that revolution 2000rpm, friction pressure 25MPa, angle between stir rod section and plate 4$^{\circ}$, projection pin diameter 4mm, traverse speed 0.5mm/sec, 1.0mm/sec, 1.5mm/sec. The experimental results as follows; 1. Welding beads were superior in traverse speed 1.0mm/sec and it were formed plenty of flash in traverse speed 0.5 mm/sec. Welding beads were rough in traverse speed 1.5mm/sec. 2. The maximum tensile strength was 211MPa in traverse speed 1.0mm/sec, in this case the strength was 85% of A5083 base metal tensile strength.

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Environmental Factors and Catch Fluctuation of Set Net Grounds in the Coastal Waters of Yeosu - 2 . Sea Water Circulation in the Vicinity of Set Net Ground - (여수연안 정치망어장의 환경요인과 어황 변동에 관한 연구 - 2 . 어장주변 해역의 해수유동 -)

  • Kim, Dong-Soo;Rho, Hong-Kil
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.30 no.3
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    • pp.142-149
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    • 1994
  • In order to investigate the environmental properties of set net grounds located in the coastal waters of Yeosu. The current in the vicinity of set net grounds was observed by drogue and current meter in 1990 and 1992. The results obtained are summarized as follows: The direction of tidal current at the north enterance of Yeosu bay was southerly in ebb and northwesterly in flood without the distiction of the neap tide and the spring tide. In spring tide the maximum Velocity of the tidal current was 68 cm/sec in ebb and 66 cm/sec in flood. In neap tide the maximum velocity of the tidal current was 37 cm/sec in ebb and 35 cm/sec in flood. And so the direction of residual current was the south ward mainly and 21 cm/sec. The direction of tidal current at set net fishing grounds was southwesterly in ebb and westerly or northwesterly in flood. Regardless of the distinction of neap and spring. The maximum velocity of the current in spring tide was 50 cm/sec in ebb and 40 cm/sec in flood and that in neap was 28 cm/sec in ebb and 25 cm/sec in flood. In spring tide the speed vector along the major axis of semidiurnal tide component was three times as large as diurnal tide. In neap tide, however, the speed vector was about 50% less then that in spring tide, and the semidiurnal tide and diurnal tide were equal in the size of current ellipse and the direction of major axis. The sea area had a southwesterly residual current. 11 cm/sec in spring tide and 7 cm/sec in neap tide. According to the result of drogue tracking, the vicinity of set net fishing ground had a southerly residual current which formed in Yeosu Bay and a weak westerly residual current toward Dolsando from Namhedo. Therefore, set net fishing ground in coastal water of Yeosu was distributed in boundary of inner water which formed from Seamjin river and offshore water supplied from the vicinity of Sorido and Yochido.

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Determination of Somatosensory Evoked Potentials(SEPs) by Posterior Tibial Nerve Stimulation in Dogs (개에서 뒤쪽 경골신경자극에 의한 Somatosensory Evoked Potentials(SEPs)의 측정)

  • 이주명;권오경;남치주
    • Journal of Veterinary Clinics
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    • v.17 no.2
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    • pp.388-394
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    • 2000
  • 이 실험은 소형견종에 대한 정상 SEPs의 범위를 알아내기 위해 실시되었다. 임상증 상이 정상인 28두를 대상으로 자극점에서 channel 1 까지의 Pl(LPI), channel 1까지의 Nl (LN1), 자극점에서 channel 2가지의 Pl(TP1), channel 2까지의 N1(TNI)의 절대잠복기와 LP1-TN1` 의 파간잠복기를 알아내기 위해서 실시하였다. 이번 실험에서 LPI, LNI, TPI, TNI의 절대잠복기 (absolute latency)의 평균값은 2.69$\pm$0.31 msec, 4.91$\pm$0.49m/sec, 4.64$\pm$0.39 msec, 5.21$\pm$0.42 msec 띠었다. LP1과 TN1 사이의 파간절대잠복기의 핑균값은 2.52$\pm$7.19 msec 이었다. 측정 치들을 속도로 변환하였을 경우 다음과 같았다. 측, LPI, LNI. Tfl, TNI 그리고 LP1-TN1 에서의 속도의 평균값은 각각 93.11$\pm$ 8.58 m/sec, 50.99$\pm$ 5.36m/sec. 80.18$\pm$ sec, 71.31$\pm$4.79m/sec그리고 49.50$\pm$3.58m/sec 이었고. 71.66m/sec, 37.79m/sec, 65.75m/ sec, 59.33 m/sec, 40.55m/sec 의 최저속도를 초과하였을 때 정상범위로 간주하였다. LPI, LNI, TPI,TN1까지의 절대잠복기와 자극전극에 시 측정전극가지의 거리 사이에는 상관관계가 있었다 LP1, LN1, TP1, TN1의 상관계수는 각각 0.621, 0.494. 0.577,0.618 이었다 요추에서 기록된 SEPs갈은 LP1의 상관계수가 LN1 보다 높았으며 흉추에서 기록된 SEPs값은 TN1의 상관계수가 TP1보다 높았다. LP1과 TN1의 파간잠복기와 channel 1과 2의 거리차이와의 상관계수는 0.571이다. 따라서 LPI, LNI. TPI, TNI그리고 LPI-TNI 들의 최저속도를 이용 하여 척수 손상 여부를 판단할 수 있다고 생각된다.

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The Study of Isokinetic Exercise Test of Trunk Rotation of 20's Male College Students (20대 남자대학생의 몸통회전에 대한 등속성운동 검사에 관한 연구)

  • Choi, Byung-Ok
    • The Journal of Korean Academy of Orthopedic Manual Physical Therapy
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    • v.12 no.2
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    • pp.21-25
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    • 2006
  • Objective: The purposes of this study are to provide fundamental data and a rehabilitation program in physical therapy. It has been operated by 30 male college students for lumbar isokinetic exercises. Method : Subjects have been carried out in an experiment by Biodex system operating isokinetic torso rotation. This performance was activated by left rotation after right rotation repeatedly. Results: 1. The mean peak torques at $60^{\circ}$/sec were $95.6{\pm}20.62$ (Rt) and $93.84{\pm}18.41$ (Lt). 2. The mean peak torques/body weight at $60^{\circ}$/sec were $69.28{\pm}28.31$ (Rt) and $67.07{\pm}27.04$ (Lt). 3. The total works in right side at $60^{\circ}$/sec and $180^{\circ}$/sec were $492.21{\pm}92.37$, $1294.97{\pm}278.96$ and in left side at $60^{\circ}$/sec and $180^{\circ}$/sec were $515.78{\pm}109.47$, $1443.74{\pm}329.67$. 4. The work to body weight ratio at $60^{\circ}$/sec was $78.30{\pm}32.37$ (Rt) and $79.93{\pm}37.30$ (Lt). 5. The average powers in right side at $60^{\circ}$/sec and $180^{\circ}$/sec were $76.75{\pm}16.69$, $117.19{\pm}29.94$ and in left side at $60^{\circ}$/sec and $180^{\circ}$/sec were $78.54{\pm}18.58$, $125.39{\pm}32.90$. 6. The work fatigue at $180^{\circ}$/sec was $21.76{\pm}14.82$ (Rt) and $19.66{\pm}26.23$ (Lt). Conclusion: In right side and left side, there was no significant difference in peak torque. peak torque/body weight, work to body-weight ratio, average power of trunk rotators at $60^{\circ}$/sec(p<0.05). However there was a significant difference in the total work of trunk rotators at $60^{\circ}$/sec(p>0.05). Also there was a significant difference in the average of trunk rotators at $180^{\circ}$/sec(p>0.05).

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Design of Modified CGA for Address Autoconfiguration and Digital Signature in Hierarchical Ad Hoc Network (개선된 CGA(Modified CGA)를 이용한 계층적 애드 혹 네트워크에서의 주소 자동 설정 및 전자 서명 제공 방안)

  • Lee, Hye-Won;Kim, Guk-Boh;Mun, Young-Song
    • Journal of KIISE:Information Networking
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    • v.33 no.2
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    • pp.175-182
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    • 2006
  • The CGA proposed by IETF working group prevents address spoofing and stealing and provides digital signature to users, but key collision problem arises. To solve this critical problem, the CGA defines the SEC field within address format, which is set to high value when high security is required and vice versa, but the CGA faces a dilemma between security and the processing time. As SEC value increases, the processing time to generate the CGA grows dramatically while key collision ratio increases if low SEC value is applied to the CGA. We propose modified CGA (MCGA) that has shorter processing time than the CGA and offers digital signature with small overheads. To solve key collision problem, we employ hierarchical ad hoc network. The MCGA is applicable to IPv6 networks as well public networks. In this paper, we design a mathematical model to analyze the processing time for MCGA and CGA first and evaluate the processing time via simulations, where the processing time for MCGA is reduced down 3.3 times when SEC value is set to 0 and 68,000 times when SEC value is set to 1. Further, we have proved that the CGA is inappropriate for both ad hoc networks and IPv6 networks when the SEC field is set to more than 3.

The Kinematical Analysis of Straddle Jump to Push up Motion on Sports Aerobics (스포츠 에어로빅스 Straddle Jump to Push up 동작의 운동학적 분석)

  • Kim, Cha-Nam
    • Korean Journal of Applied Biomechanics
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    • v.12 no.2
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    • pp.77-90
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    • 2002
  • This study serves the purpose of understanding about correct jump and landing motion through Kinematical Analysis of Straddle Jump to Push up Motion at target by four elite sports aerobics athletes have more than four years career. And further more that make good assistance for coaches effective guidance through an offer basic data and correct diagnosis, evaluate of motions. It was picture-taked by two-video camera for Straddle Jump to Push up Motions. Camera speeds are 60 frame/sec. There are Kinematical Variation elements for analysis, the displacement of COG, each angle displacement left/right of shoulder-joint, each angle displacement left/right of knee-joint and each speed left/right of tip of the toes. Every each person accomplished severaly 3 times and we have acquired this conclusion. The conclusions were as follows; 1. Each situation for displacement of COG showed low height of COG by phase 1, 4, 5(79.05${\pm}9.07,\;46.41{\pm}3.65,\;18.66{\pm}0.54cm$) and It showed high height of COG by phase 2, 3($120.80{\pm}6.13,\;148.12{\pm}9.19cm$). 2. Each displacement left, right of shoulder-joint flexion by phase 1($91.07{\pm}8.30,\;90.77{\pm}5.72$deg/sec)and It showed maximal extension angles by phase 2($102.48{\pm}10.00,\;102.39{\pm}10.51$deg/sec). in part of phase 3, left of shoulder-joint angle($94.43{\pm}4.12$deg/sec) showed flexion phase 1, the other right shoulder-joint angle(88.38${\pm}$4.98deg/sec) showed more a little lower than phase 1, in last phase that showed most low by phase 4($70.58{\pm}13.72,\;54.24{\pm}11.58$deg/sec). 3. Each displacement left, right of hip joint showed maximal extent conditions by phase 2, 3($160.35{\pm}22.68,\;1534.77{\pm}5.40$deg/sec, $150.04{\pm}12.79,\;145.54{\pm}13.00$deg/sec) beside, ankle-joint showed minimal angle by phase 1, 4($93.59{\pm}18.92,\;85.37{\pm}13.23$deg/sec, $66.60{\pm}15.77,\;80.60{\pm}16.57$deg/sec). 4. Each displacement left, right of hip joint showed maximal extent conditions by phase 2($157.15{\pm}9.13,\;163.52{\pm}8.18$deg/sec), and right of hip joint showed minimal angle by phase 3($110.87{\pm}13.81,\;77.53{\pm}8.95$deg/sec) It showed alike condition of low angle by phase 1, 4($91.04{\pm}2.31,\;96.26{\pm}2.20$deg/sec). 5. Each displacement left, right of knee-joint showed maximal extent conditions by phase 1, 3, 4($173.46{\pm}2.95,\;171.51{\pm}5.44$deg/sec, $172.24{\pm}4.49,\;171.26{\pm}0.65$deg/sec, $162.78{\pm}2.13,\;164.10{\pm}5.97$deg/sec) but It showed flexion only left of knee-joint by phase 2($164.45{\pm}7.51,\;159.38{\pm}3.48$deg/sec). 6. Each speed left, right of the tip of the toes showed most fastest when someone jumped with lift up leges by phase 1, 2($321.32{\pm}67.91,\;316.90{\pm}41.97$cm/sec, $410.06{\pm}153.06,\;399.77{\pm}189.34$cm/sec), It showed more less speed than phase 1,2 by phase 3($169.74{\pm}67.17,\;150.00{\pm}63.80$cm/sec) and It showed most slow speed than phase 1,2,3 by phase 4($87.22{\pm}34.90,\;85.72{\pm}52.23$cm/sec).

Measurement and Prediction of Autoignition Temperature of n-Butanol and sec-Butanol System (노말부탄올과 2차부탄올 계의 최소자연발화온도의 측정 및 예측)

  • Ha, Dong-Myeong
    • Fire Science and Engineering
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    • v.26 no.5
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    • pp.48-53
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    • 2012
  • The autoignition temperature (AIT) is important index for the safe handling of flammable liquids which constitute the solvent mixtures. This study measured the AITs and ignition delay time for n-Butanol+sec-Butanol system by using ASTM E659 apparatus. The AITs of n-Butanol and sec-Butanol which constituted binary system were $340^{\circ}C$ and $447^{\circ}C$, respectively. The experimental AITs of n-Butanol+sec-Butanol system were a good agreement with the calculated AITs by the proposed equations with a few A.A.D. (average absolute deviation).