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Ichthyotoxic Cochlodinium polykrikoides red tides offshore in the South Sea, Korea in 2014: II. Heterotrophic protists and their grazing impacts on red-tide organisms

  • Lim, An Suk;Jeong, Hae Jin;Seong, Kyeong Ah;Lee, Moo Joon;Kang, Nam Seon;Jang, Se Hyeon;Lee, Kyung Ha;Park, Jae Yeon;Jang, Tae Young;Yoo, Yeong Du
    • ALGAE
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    • v.32 no.3
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    • pp.199-222
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    • 2017
  • Occurrence of Cochlodinium polykrikoides red tides have resulted in considerable economic losses in the aquaculture industry in many countries, and thus predicting the process of C. polykrikoides red tides is a critical step toward minimizing those losses. Models predicting red tide dynamics define mortality due to predation as one of the most important parameters. To investigate the roles of heterotrophic protists in red tide dynamics in the South Sea of Korea, the abundances of heterotrophic dinoflagellates (HTDs), tintinnid ciliates (TCs), and naked ciliates (NCs) were measured over one- or two-week intervals from May to Nov 2014. In addition, the grazing impacts of dominant heterotrophic protists on each red tide species were estimated by combining field data on red tide species abundances and dominant heterotrophic protist grazers with data obtained from the literature concerning ingestion rates of the grazers on red tide species. The abundances of HTDs, TCs, and NCs over the course of this study were high during or after red tides, with maximum abundances of 82, 49, and $35cells\;mL^{-1}$, respectively. In general, the dominant heterotrophic protists differed when different species caused red tides. The HTDs Polykrikos spp. and NCs were abundant during or after C. polykrikoides red tides. The mean and maximum calculated grazing coefficients of Polykrikos spp. and NCs on populations of co-occurring C. polykrikoides were $1.63d^{-1}$ and $12.92d^{-1}$, respectively. Moreover, during or after red tides dominated by the phototrophic dinoflagellates Prorocentrum donghaiense, Ceratium furca, and Alexandrium fraterculus, which formed serial red tides prior to the occurrence of C. polykrikoides red tides, the HTDs Gyrodinium spp., Polykrikos spp., and Gyrodinium spp., respectively were abundant. The maximum calculated grazing coefficients attributable to dominant heterotrophic protists on co-occurring P. donghaiense, C. furca, and A. fraterculus were 13.12, 4.13, and $2.00d^{-1}$, respectively. Thus, heterotrophic protists may sometimes have considerable potential grazing impacts on populations of these four red tide species in the study area.

Changes in Nutritional Components of the Northern and Southern Types Garlic by Different Heat Treatments (열처리방법에 따른 한지형 및 난지형 마늘의 영양성분 변화)

  • Lee, Ju-Hye;Lee, Jiyoon;Whang, Jinbong;Nam, Jin-Sik;Lee, Junsoo;Kim, So-Min;Han, Hye-Kyung;Choi, Youngmin;Kim, Se-Na;Kim, Haeng Ran
    • Korean journal of food and cookery science
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    • v.32 no.3
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    • pp.245-252
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    • 2016
  • Purpose: To provide the public with nutritional information on consumption types of garlic, we evaluated the influence of heat treatment method on the nutritional contents of different species of garlic. Methods: We determined the content of general components, minerals, vitamins, and fatty acids in each species of garlic produced in Seosan and Goheung by heating with blanching or microwave roasting. Results: The results of the two-way analysis of variance test indicated that the species in particular, as well as method of heat treatment and interaction, had an influence on nutritional content. The moisture and crude fat content was higher in Southern type garlic than in Northern type garlic, while crude proteins, crude ash, and dietary fibers were more abundant in Northern type garlic than in Southern type garlic. With regard to the total mineral content, K, P, Mg, and Ca were the main components in Northern type garlic and Southern type garlic. Moreover, unsaturated fatty acids showed high levels in both Northern type garlic and Southern type garlic, with more abundant linoleic acid. Overall, Northern type garlic showed a higher content of minerals and fatty acids, while more vitamin B was present in Southern type garlic. In addition, the results indicated that the content of general components (dietary fiber excluded) was increased in both Northern type garlic and Southern type garlic upon heat treatment. Blanching resulted in increased mineral and fatty acid content in Southern type garlic and decreased content in Northern type garlic, indicative of species differences; conversely, microwave roasting contributed to an increase in the content in both Northern type garlic and Southern type garlic. Conclusion: The nutritional content of garlic is more dependent on species than the cooking method, and grilling is associated with less nutrient loss than blanching.

A Study on a God tree of Chosun Distorted in Chosun-Gersu-Nosu-Myungmok-Ji (조선거수노수명목지에 왜곡되어 있는 조선의 신목에 관한 고찰)

  • Park, Chan-Woo;Ahn, Chang-Ho;Kim, Se-Chang
    • Journal of Korean Society of Forest Science
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    • v.108 no.3
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    • pp.372-381
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    • 2019
  • This study was conducted to find proof for the hypothesis that the God tree of Chosun has been misrepresented in Chosun-Gersu-Nosu-Myungmokji (CGNM). The following results were obtained. First, it was established that 64 species and 3170 trees were recorded in CGNM. An old, big tree is classified as a God tree if linked to it there are testimonies and legends about divine elements, and it is classified as a Noble tree if linked to it there are testimonies and legends of historical elements. In total, 2632 trees of eight species were analyzed, from the Zelkova serrata, which has the greatest number of trees, to the eighth most frequent, Abies holophylla. The means of diameter at breast height (DBH), height, and age of the God and the Noble trees were calculated for each of the eight species. In seven out of eight species, the DBH and age of the Noble tree were more than those of the God tree. In addition, the height of the Noble tree was more than that of the God tree in six out of eight species. The fact that the God tree is smaller than the Noble tree, contrary to the common expectation that the Noble tree is a small size tree, was confirmed. This hypothesis was proved by the data gathered. Second, the Japanese Government-General of Korea has pursued a policy to defeat the village ritual based on the God tree being linked with superstition. For such a policy, the God tree should be small and unattractive, and it would have been good for the tree to be superstitious. The CGNM was created as explanatory material or evidence for distorting the sacredness of the God tree of Chosun. Third, CGNM compiled a chronological order of DBH data to make it easy to explain the fabricated facts that the God tree of Chosun is smaller and dwarfed compared to the Noble tree.

The taxonomic entity and distribution of Korean Sedum formosanum (Crassulaceae) revealed in 133 years (133년만에 밝혀진 한국산 주걱잎갯비름(돌나물과)의 분류학적 실체 및 분포)

  • CHOI, Seung Se;KIM, Jonghwan;KIM, Chul Hwan
    • Korean Journal of Plant Taxonomy
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    • v.50 no.4
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    • pp.377-384
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    • 2020
  • Korean Sedum formosanum N. E. Br. (Ju-geog-nip-gaet-bi-reum in Korean) was first recorded in 1887, 133 years ago. Since then, the species has not been collected and its current state has remained unclear. However, these plants were collected in 2020 and the corresponding taxonomic entity and distribution status were revealed. It is known to be distributed only in the southern region of Japan, the northern islands of the Philippines, and in Taiwan, with Taiwan being the collection site of the type specimen. However, on the basis of the findings of the fourth national natural environment survey, it has recently been established that this plant also grows in the crevices of rocks along the seashores of the Korean islands of Hataedo Island and Sangtaedo Island, Sinan-gun, Jeollanam-do. S. formosanum inhabiting Korea is a large succulent biennial that can attain a height of up to 65 cm and differs from its congeneric species in having erect follicles during the fruiting period. Notably, among the Korean Sedum species, S. formosanum is most similar to S. tosaense, although it can be distinguished from this species with respect to its monomorphic leaves that have rounded apices, and it also bears separate flowering and sterile stems. In this paper, we present a description and photographs of the Korean S. formosanum, indicate the differences between this and related species, and provide a key to related taxa.

Analysis of Genetic Variation in the Small Subunit Ribosomal RNA Gene of Euplotes Ciliates for Developing Species Diagnostic Molecular Marker (종 식별 분자 마커 개발을 위한 섬모충류 Euplotes의 small subunit ribosomal RNA 변이성 분석)

  • Kim, Sun-Young;Kim, Se-Joo;Min, Gi-Sik;Yang, Eun-Jin;Yoo, Man-Ho;Choi, Joong-Ki
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.12 no.3
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    • pp.225-233
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    • 2007
  • To verify which loop regions of 18S rRNA gene are suitable as species-specific genetic markers in ciliates, we analyzed the genetic variation of 18S rRNA gene among 9 Euplotes species (Hypotrichia : Ciliophora). In our result, no inter-specific variation was detected from V1, V3 and V5 regions, and the length of V7 and V8 are 44 bp and 79 bp, respectively, which are too short to make genetic marker. In contrast, V2 and V4 may be good candidate segments of species-specific diagnostic molecular markers because these two regions are most variable ($1.75{\sim}20.61%$) and showed good inter-specific phylogeny. Furthermore, the sequences of V2 and V4 are 123 bp and 306 bp, respectively in length which are enough to make species-specific marker.

Nocturnal Birds Detection and Ecological Characteristics through Bioacoustic Monitoring (생물음향 모니터링 기법을 이용한 야행성 조류 탐지 및 생태적 특성 분석)

  • Choi, Se-Jun;Ki, Kyong-Seok
    • Korean Journal of Environment and Ecology
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    • v.33 no.6
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    • pp.636-644
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    • 2019
  • The purpose of this study was to investigate the callings of nocturnal birds using bioacoustic recording technology to identify species and to analyze the ecological characteristics of each species. Three sites - Seoraksan National Park, National Institute of Ecology, and Mudeungsan National Park - were investigated. The investigation period was from the middle of April 2018 to early March 2019 for Seoraksan national park, from late February of 2018 to the middle of February 2019 for the National Institute of Ecology, and from the middle of February 2018 to the end of August 2018 for Mudeungsan National Park. The main research results are as follows. Firstly, nocturnal bird species identified by the survey included Caprimulgus indicus, Otus sunia, Zoothera aurea, Bubo bubo, and Strix uralensis, 5 species in total. Secondly, the breeding call period of each species was from early May to early August for C. indicus, from early April to the end of September for O. sunia, from early March to early October for Z. aurea, from late September to early February for B. bubo, and from mid-January to early March for S. uralensis. Thirdly, the mating call rhythm was between 16:00 and 10:00 on the following day for all the observed species in the three regions, and the peak time zone was from 20:00 to 06:00 on the following day. Fourthly, there was no correlation between the cumulative call frequency and the precipitation for each species. Fifthly, the mean temperature during the period when the specific calls of nocturnal birds were detected was -4.00 ℃ for S. uralensis, 2.58 ℃ for B. bubo, 13.66 ℃ for Z. aurea, 19.50 ℃ for O. sunia, and 20.77 ℃ for C. indicus. The ANOVA results showed that there was a significant difference in mean temperature for the calling by species and that the mean temperature was S. uralensis, B. bubo, Z. aurea, and O. sunia-C. indicus, in the ascending order, for 4 groups in total. The period of the specific mating calls confirmed by the study is a period in which the frequency of calls was the highest among the periods when the specific calls were detected. Since it is associated with the known mating period of each species, the period of the high frequency of calls confirmed by the bioacoustic monitoring can be regarded as the mating season. This study is meaningful in that it is the early research that has used the bioacoustic recording technology to identify species and ecological characteristics of species of nocturnal birds in Korea.

Quantitative Determination of Psoralen and Angelicin from Some Medicinal Herbs by High Performance Liquid Chromatography

  • Dong, Nguyen-Thanh;Bae, Ki-Hwan;Kim, Young-Ho;Hwang, Gwi-Seo;Heo, Ok-Soon;Kim, Se-Eun;Kang, Jong-Seong
    • Archives of Pharmacal Research
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    • v.26 no.7
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    • pp.516-520
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    • 2003
  • A reversed-phase high performance liquid chromatographic method was developed to determine the contents of psoralen and angelicin from some medicinal herbs. The optimum eluent for chromatography was 20 v/v% acetonitrile in water on a Zorbax 300SB $C_{18}$ column. The identification was carried out by comparing the retention time and mass spectra of the relevant peaks with their standards. The variation of the concentration of psoralen and angelicin was wide between different species. The seeds of Psoralea corylifolia showed the highest contents of psoralen (7.8 mg/g) and angelicin (2.3 mg/g) among the tested herbs.

PARAMETRIC INVESTIGATIONS ON THE DOUBLE DIFFUSIVE CONVECTION IN TRIANGULAR CAVITY

  • Kwon, SunJoo;Oh, SeYoung;Yun, Jae Heon;Chung, Sei-Young
    • Journal of the Chungcheong Mathematical Society
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    • v.20 no.4
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    • pp.419-432
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    • 2007
  • Double-diffusive convection inside a triangular porous cavity is studied numerically. Galerkin finite element method is adopted to derive the discrete form of the governing differential equations. The first-order backward Euler scheme is used for temporal discretization with the second-order Adams-Bashforth scheme for the convection terms in the energy and species conservation equations. The Boussinesq-Oberbeck approximation is used to calculate the density dependence on the temperature and concentration fields. A parametric study is performed with the Lewis number, the Rayleigh number, the buoyancy ratio, and the shape of the triangle. The effect of gravity orientation is considered also. Results obtained include the flow, temperature, and concentration fields. The differences induced by varying physical parameters are analyzed and discussed. It is found that the heat transfer rate is sensitive to the shape of the triangles. For the given geometries, buoyancy ratio and Rayleigh numbers are the dominating parameters controlling the heat transfer.

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Modeling of Piston Crevice Hydrocarbon Oxidation in SI Engines (전기점화 기관 간극 체적 내 미연탄화수소의 산화 모델링)

  • Choi, Hoi-Myung;Kim, Se-Jun;Min, Kyung-Doug
    • Proceedings of the KSME Conference
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    • 2001.06d
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    • pp.884-889
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    • 2001
  • Combustion chamber crevices in SI engines are identified as the largest contributor to the engine-out hydrocarbon emissions. The largest of crevice region is the piston ring pack crevice. To predict and understand the oxidation process of piston crevice hydrocarbons, a 3-dimensional numerical simulation method was developed. A engine shaped computational mesh with moving grid for piston and valve motions was constructed. And a 4-step oxidation model involving 7 species was used and the 16 coefficients in the rate expressions were optimized based on the results from a detailed chemical kinetic mechanism for the oxidation condition of engine combustion chamber. Propane was used as a fuel in order to eliminate oil layer absorption and liquid fuel effect.

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Development of Geno-chromatographic Assay System for Salmonella species

  • Kim, Seok-Ha;O, Hong-Il;Jo, Jeong-Hwan;Baek, Se-Hwan
    • 한국생물공학회:학술대회논문집
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    • 2001.11a
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    • pp.827-828
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    • 2001
  • We developed a geno-chromatographic assay system based on hybridization between target DNA (e.g., amplified product from PCR) and a capture probe immobillized on a predetermined site of membrane. This system can offer a rapid detection of a gene sequence specific to a certain type of cells as well as simplicity of the analytical procedure. Such performances were demonstrated by utilizing Salmonella typhimurium as model analyte.

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