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Analysis of Genetic Variation in the Small Subunit Ribosomal RNA Gene of Euplotes Ciliates for Developing Species Diagnostic Molecular Marker  

Kim, Sun-Young (Department of Oceanography, Inha University)
Kim, Se-Joo (Department of Biological Sciences, Inha University)
Min, Gi-Sik (Department of Biological Sciences, Inha University)
Yang, Eun-Jin (Marine Environment Research Department, KORDI)
Yoo, Man-Ho (Department of Oceanography, Inha University)
Choi, Joong-Ki (Department of Oceanography, Inha University)
Publication Information
The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY / v.12, no.3, 2007 , pp. 225-233 More about this Journal
Abstract
To verify which loop regions of 18S rRNA gene are suitable as species-specific genetic markers in ciliates, we analyzed the genetic variation of 18S rRNA gene among 9 Euplotes species (Hypotrichia : Ciliophora). In our result, no inter-specific variation was detected from V1, V3 and V5 regions, and the length of V7 and V8 are 44 bp and 79 bp, respectively, which are too short to make genetic marker. In contrast, V2 and V4 may be good candidate segments of species-specific diagnostic molecular markers because these two regions are most variable ($1.75{\sim}20.61%$) and showed good inter-specific phylogeny. Furthermore, the sequences of V2 and V4 are 123 bp and 306 bp, respectively in length which are enough to make species-specific marker.
Keywords
Euplotes; molecular marker; small subunit ribosomal RNA (18S rRNA); variable region; V2; V4;
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