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Isolation and Characterization of a Diesel-Degrading Bacterium, Gordonia sp. SD8 (디젤 분해 세균 Gordonia sp. SD8 분리 및 특성)

  • Hong, Sun-Hwa;Kim, Ji-Young;Cho, Kyung-Suk
    • Microbiology and Biotechnology Letters
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    • v.38 no.3
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    • pp.335-339
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    • 2010
  • A diesel-degrading bacterium, Gordonia sp. SD8, was isolated from soil contaminated with petroleum, and its diesel degradation was characterized in a soil as well as a liquid culture system. SD8 could grow in the mineral salt medium supplemented with diesel as a sole carbon and energy source. The maximum specific growth rate ($0.67{\pm}0.05\;d^{-1}$) and diesel degradation rate ($1,727{\pm}145$ mg-TPH $L^{-1}\;d^{-1}$) of SD8 showed at 20,000 mg-TPH $L^{-1}$ and $30^{\circ}C$, and then this bacterium could degrade high strength of diesel of 40,000 mg-TPH $L^{-1}$. The residual diesel concentration in the inoculated soil with SD8 was 3,724 mg-TPH kg-dry $soil^{-1}$ after 17 days, whereas the diesel concentration in the non-inoculated soil was $8,150{\pm}755$ mg-TPH kg-dry $soil^{-1}$. These results indicate that Gordonia sp. SD8 can serve as a promising microbial resource for the bioremediaion of contaminated soil with petroleum hydrocarbons including diesel.

Cytotoxicity of SD-994 from Artemisia argyi against L1210 Cells with Concomitant Induction of Antioxidant Enzymes (황해쑥 추출정제물 SD-994의 L1210암세포에 대한 세포독성과 항산화효소의 유발)

  • 정대영;하혜영;김안나;이승민;민태진;박시원
    • YAKHAK HOEJI
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    • v.44 no.3
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    • pp.213-223
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    • 2000
  • SD-994 was prepared from methanol extract of Artemisia argyi by stepwise purification of solvent partioning and silica gel chromatography. In the course of this purification, fractions obtained at each step were investigated for their cytotoxicities against L1210 cells. Fractions A~G prepared from chloroform fraction showed considerable cytotoxicities raging 40~90% against L1210 cells. Subfractions I~IX obtained from fraction A exhibited various cytotoxicities and subfraction I (SD-994) was found to be the most effective compound. $IC_{50}$ values of SD-994 were measured to be $0.5{\;}{\mu\textrm{g}}/ml and less than $0.05{\;}{\mu\textrm{g}}/ml against L1210 cells and normal lymphocytes, respectively: When SD-994 was added to L1210 cell as cytotoxic agent, significantly increased amount of superoxide ($O_2^-$) and dramatically augmented activities of superoxide dismutase (SOD), specially MnSOD and glutathione peroxidase (GPx) were observed according to the concentration and incubation time. Whereas, in case of normal lymphocytes under the same condition, cytotoxicities were not apparent and the generation of superoxide ($O_2^-$) or the activity changes of SOD and GPx were insignificant. These results together indicate that the cytotoxic action of SD-994 against L1210 cell may be achieved via necrosis and/or apoptosis induced by reaction oxygen species which could not probably be completely abolished even by drastically increased antioxidant enzymes, SOD and GPx activities.

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Microstructure, Hardness and Tensile Properties of 600 MPa-Grade High-Strength and Seismic Resistant Reinforcing Steels (600 MPa급 고강도 일반 및 내진 철근의 미세조직, 경도와 인장 특성)

  • Seo, Ha-Neul;Lee, Sang-In;Hwang, Byoungchul
    • Korean Journal of Materials Research
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    • v.27 no.9
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    • pp.477-483
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    • 2017
  • This present study deals with the microstructure and tensile properties of 600 MPa-grade high strength and seismic resistant reinforcing steels. The high strength reinforcing steel (SD 600) was fabricated by Tempcore processing, while the seismic resistant reinforcing steel (SD 600S) was air-cooled after hot-rolling treatment. The microstructure analysis results showed that the SD 600 steel specimen consisted of a tempered martensite and ferrite-pearlite structure after Tempcore processing, while the SD 600S steel specimen had a fully ferrite-pearlite structure. The room-temperature tensile test results indicate that, because of the enhanced solid solution and precipitation strengthening caused by relatively higher contents of C, Mn, Si and V in the SD 600S steel specimen, this specimen, with fully ferrite-pearlite structure, had yield and tensile strengths higher than those of the SD 600 specimen. On the other hand, the hardness of the SD 600 and SD 600S steel specimens changed in different ways according to location, dependent on the microstructure, ferrite grain size, and volume fraction.

Effect of Moxibustion on Urinary Incontinence in Stroke Patients - pilot study - (구(뜸)요법이 뇌졸중환자의 요실금에 미치는 효과 - 예비조사 -)

  • Kim, Byung-Eun;Lee, Hyang-Yeon
    • Journal of East-West Nursing Research
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    • v.7 no.1
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    • pp.82-93
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    • 2002
  • The purpose of this study was to investigate the effect of Moxibustion on urinary incontinence, voiding performance, muscle strength of the hip and ADL in stroke patients. The subjects of this study were 20 stroke patients hospitalized at the division of oriental medicine in K university Hospital in the period from December 26, 2001 to March 15, 2002. The research design was one group pretest-posttest design. The pretest and posttest included measuring frequency of incontinence, voiding performance score, healthy and paralytic muscle strength of the hip, and the performance level for ADL after Moxibustion for one week. Data was analyzed using the SPSS package program with Wilcoxon Signed Ranks test. The result of this study were as follows : 1) The frequency of incontinence was decreased from 3.80(SD=3.41) to 2.10(SD=2.10) after Moxibustion, and that was statistically significant(z=-3.803, p=.000). 2) The score of voiding performance was increased from 22.15(SD=12.04) to 30.65(SD=14.73) after Moxibustion, and that was statistically significant(z=-3.828, p=.000). 3) The healthy muscle strength of the hip was increased from 25.97(SD=26.87) to 46.00 (SD=40.75) after Moxibustion, and that was statistically significant(z=-3.183, p=.001). 4) The paralytic muscle strength was increased from 17.42(SD=27.04) to 33.58(SD=37.28) after Moxibustion, and that was statistically significant(z=-2.315, p=.021). 5) The performance level for ADL was increased from 17.15(SD=9.52) to 24.60(SD=13.38) after Moxibustion, and that was statistically significant(z=-3.416, p=.001). According to the results of this study, Moxibustion is effective for decreasing urinary incontinence, and improving voiding performance, muscle strength of the hip and ADL. Consequently, these findings showed that Moxibustion is effective in improvement of urinary incontinence in stroke patients.

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Use of Paper Mill Sludge and Briquet Ash as Root Zone Soil Mixtures for Thrfgrass Culture (잔디상토로서의 제지스럿지와 연탄재 이용에 관한 연구)

  • 구자영;김태일;안주원
    • Asian Journal of Turfgrass Science
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    • v.6 no.1
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    • pp.11-22
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    • 1992
  • To determine the use of waste materials as root zone soil mixtures for turfgrass culture, the effects of paper mill sludge and briquet ash on physical and chemical properties of soil and growth of turfgrasses were examined. Three turfgrass species of zoysiagrass(Zoysia japonicaSteud.). kentycky bluegrass(Poa pratensis L. 'Ram I') and creeping bentgrass(Agrostis panistris Huds 'Persucross') were cultured in 32cm diameter plastic pots containing various soil mixtures. The basic ingredients used for mixtures included sand(SD), field soil(SL), paper mill sludge(PS), sphagnum peat moss(PM) and briquet ash(BA). Seven combinations using these ingreients were mixed in different percentage by volume as follows: SD+SL+PM(80:10.10), SH+SL+PS(80:10:10), SD-PM(80:20), SD+PS (80:20), SD+BA(80:20), SD+BA+PM(60:20:20) and SD+BA+PS(60:20:20). 1. Paper mill sludge showed pH of 6.6, more than 30% of organic matter content, and higher concentrations of total N, P, k, Ca, Mg and CEC. Bulk density, fild moisture capacity and electrical conductivity of soil mixtures were increased by the comimation of 10~20% PS by volume. 2. Briquet ash showed pH of 8.0, and higher levels of P, k, Ca and Mg than those of field soiks. Bulk density, field moisture capacity and hardenss of soil mixtures were increased but vertical water flow rate and electrical conductivity were decreased by the combination of 20% BA by volume. 3. Phytotoxic effects of PS and BA on growth of turfgrasses were not found. Shoot growth of all three species was higher in soil combination of SD+BA+PS than that of SD+SL+PM added with fertilizer. However, root growth was better in soil mixtures combined with PM. Soil mixtureomposed of 60% SD, 20% BA and 20% PS by volume was most effective on growth of all three species. 4. Paper mill sludge resulted in higher N level in the leaf tissue. The contents of heavy metals such as Cd and Ph did not vary significantly among soil mixtures and species. However, the Mn level was 2~3 times higher in plants growh in mixtures containing PM compared with others, and especially it was higher in creeping bentgrass than other species.

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HMM with Global Path constraint in Viterbi Decoding for Insolated Word Recognition (전체 경로 제한 조건을 갖는 HMM을 이용한 단독음 인식)

  • Kim, Weon-Goo;Ahn, Dong-Soon;Youn, Dae-Hee
    • The Journal of the Acoustical Society of Korea
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    • v.13 no.1E
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    • pp.11-19
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    • 1994
  • Hidden Markov Models (HMM's) with explicit state duration density (HMM/SD) can represent the time-varying characteristics of speech signals more accurately. However, such an advantage is reduced in relatively smooth state duration densities or ling bounded duration. To solve this problem, we propose HMM's with global path constraint (HMM/GPC) where the transition between states occur only within prescribed time slots. HMM/GPC explicitly limits state durations and accurately describes the temproal structure of speech simply and efficiently. HMM's formed by combining HMM/GPC with HMM/SD are also presented (HMM/SD+GPC) and performances are compared. HMM/GPC can be implemented with slight modifications to the conventional Viterbi algorithm. HMM/GPC and HMM/SD_GPC not only show superior performance than the conventional HMM and HMM/SD but also require much less computation. In the speaket independent isolated word recognition experiments, the minimum recognition eror rate of HMM/GPC(1.6%) is 1.1% lower than the conventional HMM's and the required computation decreased about 57%.

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Structural Performance of Mortar-filled Sleeve Splice for SD500 High-strength Reinforcing Bar under Cyclic Loading (반복하중을 받는 SD500 고강도 철근용 모르타르 충전식 슬리브 철근이음의 구조성능)

  • Kim, Hyong-Kee
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.12 no.6
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    • pp.180-192
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    • 2008
  • The purpose of this research is to evaluate the structural performance of mortar-filled ductile cast iron sleeve splice developed for SD500 high-strength reinforcing bar under cyclic loading. The test variables adopted in this study are the development length of bar, compressive strength of mortar, bar size, sleeve types and others. In this research, it is showed that the mortar-filled sleeve splice for SD500 high-strength bar satisfied the structural performance required in ACI, AIJ code as well as domestic code. Also the results of experimental research presented in this paper provided basic engineering data for developing a reasonable design method of mortar-filled sleeve splice for SD500 high-strength reinforcing bar.

Effects of Stocking Density and Dissolved Oxygen Concentration on the Growth and Hematology of the Parrotfish Oplegnathus fasciatus in a Recirculating Aquaculture System (RAS) (순환여과시스템에서 사육밀도와 용존산소 농도가 돌돔(Oplegnathus fasciatus)의 성장과 혈액성상에 미치는 영향)

  • Kim, Pyong-Kih
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.44 no.6
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    • pp.747-752
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    • 2011
  • The parrotfish Oplegnathus fasciatus is a sub-tropical species that is difficult to culture during the winter in South Korea. As a result, a recirculating aquaculture system (RAS) was developed to rear parrotfish. This study investigated the effects of stocking density and dissolved oxygen (DO) concentration on the growth and hematology of the parrotfish in the RAS. The experimental stocking densities were 5 (SD05), 10 (SD10), 15 (SD15), and 20 kg/m3 (SD20) total body weight to tank water volume. As the stocking density increased, the mean weight gain, feed efficiency, and specific growth rate tended to decrease. However, SD10 and SD15 did not differ statistically from SD05 in feed efficiency (P>0.05). Although better growth was achieved in SD05, SD10 and SD15 appeared to be acceptable, practically and economically, in terms of feed efficiency. The experimental DO concentration ranges were 3-4 (DO3), 5-6 (DO5), and 7-8 mg/L (DO7). The mean weight gain and survival tended to increase with the DO concentration, but there were no differences among treatments (P>0.05). Although the feed efficiency did not differ among the treatments, the specific growth rates and daily feed intake increased with the DO concentration and were the highest in DO7 (P<0.05). The cortisol concentration was the highest in DO3 (P<0.05), while there was no difference between DO5 and DO7 (P>0.05).

Homology of Scytalone Dehydratase Melanin Gene in Venturia nashicola (배검은별무늬병균의 Scytalone Dehydratase 멜라닌유전자의 상동성)

  • Yun, Yeo Hong;Yoon, Seong Kwon;Son, Seung Yeol;Kim, Seong Hwan
    • The Korean Journal of Mycology
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    • v.41 no.3
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    • pp.200-204
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    • 2013
  • Dihydroxynaphthalene (DHN) melanin is known to be present in some ascomycete fungi. To verify the type of melanin in Venturia nashicola that cause scab on pear, we investigated scytalone dehydratase (SD) gene, one of DHN melanin genes, from 11 isolates of V. nashicola from different provinces in Korea and Japan. Through PCR approach, 429 bp amplicon was produced from the 11 isolates and sequenced. All of the PCR-amplified sequences were determined as SD gene through GenBank database search. All the determined sequences were composed of an intron and two exons coding for 122 amino acids of SD. The homology of SD gene was 100% among the 11 isolates. Sequence identity of the predicted SD protein of 122 amino acids ranged 69 to 73% with other fungi. Our results proved that V. nashicola operates DHN melanin pathway.