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http://dx.doi.org/10.4489/KJM.2013.41.3.200

Homology of Scytalone Dehydratase Melanin Gene in Venturia nashicola  

Yun, Yeo Hong (Department of Microbiology, Dankook University)
Yoon, Seong Kwon (Department of Microbiology, Dankook University)
Son, Seung Yeol (Department of Microbiology, Dankook University)
Kim, Seong Hwan (Department of Microbiology, Dankook University)
Publication Information
The Korean Journal of Mycology / v.41, no.3, 2013 , pp. 200-204 More about this Journal
Abstract
Dihydroxynaphthalene (DHN) melanin is known to be present in some ascomycete fungi. To verify the type of melanin in Venturia nashicola that cause scab on pear, we investigated scytalone dehydratase (SD) gene, one of DHN melanin genes, from 11 isolates of V. nashicola from different provinces in Korea and Japan. Through PCR approach, 429 bp amplicon was produced from the 11 isolates and sequenced. All of the PCR-amplified sequences were determined as SD gene through GenBank database search. All the determined sequences were composed of an intron and two exons coding for 122 amino acids of SD. The homology of SD gene was 100% among the 11 isolates. Sequence identity of the predicted SD protein of 122 amino acids ranged 69 to 73% with other fungi. Our results proved that V. nashicola operates DHN melanin pathway.
Keywords
DHN melanin; Pear scab; Scytalone dehydratase gene; Venturia nashicola;
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1 Henson, J. M., Butler, M. J. and Day, A. W. 1999. The dark side of the mycelium: Melanins of phytopathogenic fungi. Annu Rev Phytopathol. 37:447-471.   DOI   ScienceOn
2 Howard, R. J. and Ferrai, M. A. 1989. Role of melanin in appressorium function. Exp. Mycol. 13:403-418.   DOI
3 Kim, S. H., Uzunovic, A. and Breuil, C. 1999. Rapid detection of Ophiostoma piceae and O. quercus in stained wood by PCR. Appl. Environ. Microbiol. 65:287-290.
4 Kimura, M. 1980. A simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequence. J. Mol. Evol. 16:111-120.   DOI   ScienceOn
5 Köller, W., Wilcox, W. F., Barnard, J., Jones, A. L. and Braun, P. G. 1997. Detection and quantification of resistance of Venturia inaequalis populations to sterol demethylation inhibitors. Phytopathology 87:184-190.   DOI   ScienceOn
6 Kubo, Y., Takano, Y., Endo, N., Yasuda, N., Tajima, S. and Furusawa, I. 1996. Cloning and structural analysis of the melanin biosynthesis gene SCD1 encoding scytalone dehydratase in Colletotrichum lagenarium. Appl. Environ. Microbiol. 62:4340-4344.
7 Kwon, S. M., Yeo, M. I., Choi, S. H., Kim, G. W., Jun, K. J. and Uhm, J. Y. 2010. Reduced sensitivities of the pear scab fungus (Venturia nashicola) collected in ulsan and naju to five ergosterol- biosynthesis-inhibiting fungicides. Res. Plant Dis. 16:48-58.   DOI   ScienceOn
8 Perpetua, N. S., Kubo, Y., Yasuda, N., Takano, Y. and Furusawa, I. 1996. Cloning and characterization of a melanin biosynthetic THR1 reductase gene essential for appressorial penetration of Colletotrichum lagenarium. Mol. Plant-Microbe Interact. 9:323-329.   DOI   ScienceOn
9 Swofford, D. L. 2002. PAUP: Phylogenetic analysis using parsimony (*and other methods). Version 4.0 b10. Sinauer associates, Sunderland, MA.
10 Thind, T. S., Clerjeau, M. and Olivier, J. M. 1986. First observation on resistance in Venturia inaequalis and Guignardia bidwelii to ergosterol-biosynthesis inhibitors in France. Proc. 1986 Brit. Crop Prot. Conf. 2:491-498.
11 Barug, D. and Kerkenaar, A. 1984. Resistance in mutagen induced mutants of Ustilago maydis to fungicides which inhibit ergo- sterol biosynthesis. Pestic. Sci. 15:78-84.   DOI   ScienceOn
12 Wang, H. L., Kim, S. H. and Breuil, C. 2001. A scytalone dehydratase gene from Ophiostoma floccosum restores the melanization and pathogenicity phenotypes of a melanin-deficient Colletotrichum lagenarium mutant. Mol. Genet. Genomics. 266:126-132.   DOI   ScienceOn
13 Woloshuk, C. P., Sisler, H. D., Tokousbalides, C. M. and Dutky, S. R. 1980. Melanin biosynthesis in Pyricularia oryzae: Site of tricyclazole inhibition and pathogenicity of melanin-deficient mutants. Pestic. Biochem. Physiol. 14:256-264.   DOI
14 Zeun, R. and Buchenauer, H. 1985. Effect of tricyclazole on production and melanin contents of sclerotia of Botrytis cinerea. Phytopathol. Z. 112:259-267.   DOI
15 Bell, A. A. and Wheeler, M. H. 1986. Biosynthesis and functions of fungal melanins. Annu. Rev. Phytopathol. 24:411-451.   DOI   ScienceOn
16 Butler, M. J. and Day, A. W. 1998. Fungal melanins: a review. Can. J. Microbiol. 44:1115-1136.   DOI   ScienceOn
17 Caesar-TonThat, T. C., Kloeke, F. V., Geesey, G. G. and Henson, J. M. 1995. Melanin production by a filamentous soil fungus in response to copper and localization of copper sulfide by sulfide- silver staining. Appl. Environ. Microbiol. 61:1968-1975.