• 한국약용작물학회지
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• 제12권5호
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• pp.372-377
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• 2004

Scopolia parviflora Nakai, a rare and endangered species, is the sole plant producing tropane alkaloids (TA) among the Korean native species. In order to enhance TA productivity the SP72 root line was selected by screening 100 of root line, and the optimal culture media for root growth and TA production were investigated with the SP72 roots. Based on the several media, SH and 2B5 medium were determined as growth medium and White and NN medium as production medium. Among the four combinations of two-stage culture, 2BN (2B5 as growth medium plus NN as production medium) showed more enhanced root growth and TA production as compared with production media of White and NN medium and growth media of SH and 2B5 medium, respectively. However, bubble column bioreactor (BCB) cultures applying two-stage culture did not reveal the effective results despite of the each successful operation of two-stage culture in conical flasks and BCB cultures.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.483-486
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• 2001

Growth and tropane alkaloids(hyoscyamine and scopolamine) contents were studied in root cultures from Scopolia paruiflora. All excised roots grew well, even without an addition of a growth regulators. Scopolia roots could be divided scopolamine-rich species. Among the culture media, SH medium was the best for tropane alkaloids production from the hairy roots, whereas the growth increased in SH medium. In precusor experiments, feeding of hyoscyamine was promotive for scopolamine formation. Abiotic elicitors made a slightly production promotion effect.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2009년도 심포지엄 및 춘계학술발표회
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• pp.171-172
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• 2009

• 한국약용작물학회지
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• 제11권5호
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• pp.358-363
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• 2003

미치광이풀의 모상근 뱌양에 의해 의학적으로 중요한 물질인 scopolamine과 hyoscyamine 생산을 증진시키기 위해 3종의 곰팡이와 1종의 효모 유래의 생물학적 elicitor를 supernatant와 homogenate의 형태로 처리하였다. Elicitor 중 Candida albicans는 supernatant와 homogenate 모두 scopolamine 생산성을 증가시켰다. Fusarium solani의 homogenate와 C. albicans의 supernatant 처리로 hyoscyamine 함량이 증가되었다. 그러나 나머지 fungus strain도 대조구에 비해 tropane alkaloids의 생산을 다소 증가시켰다. 본 연구에서는 C. albicans가 tropane alkaloids 생산에 적합한 elicitor임으로 구명되었다. 본 연구 결과는 미치광이풀 모상근 대량배양에 의한 tropane alklaoids 대량생산에 기여할 것이다.

• Journal of Microbiology and Biotechnology
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• 제15권1호
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• pp.91-98
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• 2005

The hyoscyamine 6${\beta}$-hydroxylase (h6h) gene was introduced into the genome of Scopolia parviflora through the Agrobacterium rhizogenes binary vector system. The enzyme was expressed ally and tissue specific selectively in roots, resulting in five transgenic hairy root lines. The presence of the h6h gene in kanamycin-resistant hairy roots and its overexpression were confirmed by polymerase chain reaction (PCR), Northern blotting, and Western blotting, respectively. In the transgenic hairy root lines which constitutively expressed the H6H enzyme, hyoscyamine and scopolamine accumulated in high concentration. Among the transgenic hairy root lines that expressed the H6H enzyme, only two were more productive. The levels of tropane alkaloids in transgenic hairy root varied greatly: The best transgenic line (#5) contained 8.12 mg of scopolamine per g dry weight, which produced the compound three times more than wild-type root. These results suggest a possibility of improving the yield of tropane alkaloids in hairy root lines by genetic and metabolic engineering.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2002년도 춘계학술대회
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• pp.135-135
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• 2002

• 한국작물학회:학술대회논문집
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• 한국작물학회 2021년도 춘계학술대회
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• pp.113-113
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• 2021

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.321-323
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• 2003

Hyoscyamine and scopolamine are the pharmaceutically valuable anticholinergic drugs. The aim of our study is to increase the contents of both hyoscyamine and scopolamine by means of elicitation. Various biotic elicitors derived from 3 fungi and 1 yeast were inoculated to the hairy root cultures. When homogenate and supernatant of elicitors treated with hairy root cultures, that of yeast elicitor was only increased scopolamine production. However, all of the fungal elicitors did not influenced the productions of scopolamine and hyoscyamine. Our results will contribute to mass production of tropane alkaloids by hairy root cultures of Scopolia parviflora.

• 한국약용작물학회지
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• 제12권5호
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• pp.378-383
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• 2004

Scopolamine and hyoscyamine are important anticholinergic compounds. To increase the productivity, we have selected various elicitors and developed culture system using a bubble column bioreactor (BCB). As the same manner of elicitation in flask cultures, the elicitors were introduced into BCB cultures and the productivity was investigated. Except the bacterial elicitor of Staphyllococcus aureus, the elicitors inhibited hyoscyamine production. In scopolamine production, the elicitors revealed different responses from the results obtained in flask cultures. The elicitors of KCl and Candida albicans less increased the production than flask cultures. However, methyl jasmonate and S. aureus showed stronger positive effects on tropane alkaloid production. In particular, S. aureus was the most effective elicitor on scopolamine production and the elicitor resulted in the highly increased production, approximately 10 times higher than the control culture.

• KSBB Journal
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• 제17권6호
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• pp.525-530
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• 2002

멸종위기의 자생식물인 미치광이풀로부터 중요 의약품인 tropane alkaloid를 대량 생산하기 위한 연구의 일환으로 모상근의 최적 배양조건 및 XAD를 이용한 물질 생산 최적 조건을 확립하였다. 모상근은 표면 살균된 지하경을 1.0 mg/1 IBA가 첨가된 B5배지에 배양하여 유도하였으며, 다양한 배양조건에서 생장된 모상근의 hyoscyamine과 scopolamine 함량은 HPLC로 분석하였다. 모상근의 생장에 적합한 배지로는 SH배지로 구명되었고, tropane alkaloid 생산을 위한 최적 배지로는 l/2B5배지로 나타났다. 모상근 생장에 가장 좋은 생장조절제로는 NAA로 나타났으며, 물질생산에는 생장조절제가 첨가되지 않은 배지에서 좋았다. 모상근 생장에 적합한 탄소원으로는 3% sucrose였으며, 물질생산에는 5% scurose가 가장 좋았다. 모상근의 생장에 가장 양호한 접종농도는 0.5 g 였으며, 물질생산에는 1 g 처리구에서 좋았다. XAD 처리는 tropans alkaloid 생산성을 증가시켰으며, 배지내 물질방출을 유도하였으며, 총생산 물질의 50-80%를 회수하는 것으로 나타났다. 본 연구 결과는 미치광이풀 모상근 배양을 통한 tropane alkaloid 생산을 위한 생물공정 발전에 기여할 것이다.