Browse > Article

Optimal Culture Conditions and XAD Resin on Tropane Alkaloid production in Scopolia parviflora Hairy Root Cultures  

정희영 (경상대학교 산림과학부)
강민정 (경상대학교 산림과학부)
강영민 (경상대학교 산림과학부)
윤대진 (경상대학교 응용생명과학부)
박정동 (경상대학교 응용생명과학부)
정영관 (경상대학교 농업생명과학연구원)
최명석 (경상대학교 산림과학부)
Publication Information
KSBB Journal / v.17, no.6, 2002 , pp. 525-530 More about this Journal
Abstract
The optimum culture conditions for tropane alkaloid production in hairy root cultures of Korea native Scopolia paviflora Nak. were investigated. Hairy root was induced from the rhizome of the mother plant on B5 medium containing 1.0 mg/L IBA. Among the culture media examined, 1/2 B5 medium was the best for tropane alkaloid production, whereas the growth of hairy root increased in SH medium. The best result on the growth of hairy root was obtained in 1.0 mg/L NAA, and tropane alkaloid production was obtained in plant growth regulator-free medium. Of the carbone sources tested, 3% sucrose promoted the growth of hairy root, whereas 5% sucrose increased tropane alkaloid production. Optimum inoculum densities for root growth and tropane alkaloid production were 0.5 g and 1 g, respectively. The addition of XAD resins (1 % w/v) to hairy root cultures led to increases in tropans alkaloid production, and the release of alkaloid into the medium and its adsorption by the resin accounted for about 50 to 80％ of total production. It is concluded that optimized culture conditions and the addition of XAD resins could be used in the development of a bioprocess for tropane alkaloid production in hairy root cultures of S. paviflora Nak.
Keywords
Scopolia parviflora Nak.; tropane alkaloid; hairy roots; HPLC; XAD resin;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Nutrient requirements of suspension cultures of soybean root cells /
[ Gamborg,O.L.;R.A.Miller;K.Ojima ] / Exp. Cell Res.   DOI   ScienceOn
2 Haploid plants from pollen grains /
[ Nitsch,J.P.;C.Nitsch ] / Science   DOI   ScienceOn
3 Tropane alkaloid production in cultured cells of Duboisia /
[ Endo,T.;Y.Yamada ] / Phytochem.
4 Medium and techniques for induction and growth of monocotyledonus and dicotyledonous plant cell cultures /
[ Schenk,R.U.;A.C.Hildebrandt ] / Can. J. Bot.   DOI
5 The biosynthetic relationship between littorine and hyoscyamine in transformed roots of Datura stramonium /
[ Zabetakis,I.;R.Edwards;J.T.G.Hamilton;D.O'Hagan ] / Plant Cell Rep.   DOI   ScienceOn
6 Production of tropane alkaloids in cultured cells of Hyoscyamus niger /
[ Yamada,Y.;T.Hashimoto ] / Plant Cell Rep.   DOI
7 Adsorbent culture of tabacco cell sustensions with different adsorbants /
[ Maisch,R.;B.Kroop;R.Beiderbeck ] / Z. Naturforsch.
8 Growth and hyoscyamine production of hairy root cultures of Datura stramonium in a modified sirred tank reactor /
[ Hilton,M.G.;M.J.C.Rhodes ] / Appl. Microbiol. Biotechnol.
9 /
[ White,P.R. ] / The Cultivation of Animal and Plant Cells(2nd ed.)
10 Plant cell biotechnology for the production of alkaloids present status and prospects /
[ Verpoorte,R.R.;J.S.van der Heijden ] / J. Nat Prod.   DOI   ScienceOn
11 Commercially-feasible micropropagation of mountain laurel(Kalmia latifolia) by use of shoot tip culture /
[ Lloyd,G.;B.McCown ] / Prc. Inc. Proc, Int. Plant Prop. Soc.
12 A revised medium for rapid growth and bioassays with tobacco tissue culture /
[ Murashige,E.;F.Skoog ] / Physiol. Plant.   DOI
13 Transgenic crops for improved pharmaceutical products /
[ Oksman-Caldentey K.M.;R.Hiltunen ] / Field Crops Res.   DOI
14 Scopolamine and hyoscyamine production by hairy root cultures of Brugmansia candida: influence of calcium chloride, hemicellulase and theophylline /
[ Pitta-Alvarez,S.I.;T.C.Spollansky;A.M.Giulietti ] / Biotechnol. Lett.   DOI   ScienceOn
15 Commercial applications and economic aspects of mass plant cell culture /
[ Fowler,M.W.;S.H.Mantell(ed.);H.Smith(ed.) ] / Plant Biotechnology
16 Berberine production by cell cultures of cork tree(Phellodendron amurence Rupr.) /
[ Choi,M.S.;D.I.Shin;Y.G.Park ] / Kor. J. Pharmacogn.
17 Regulation of product synthesis in cell cultures of Catharanthus roseus(L) G. Don. growth-related indole alkaloid accumulation in batch cultures /
[ Stafford,A.;L.Smith;M.W.Fowler ] / Plant Cell Tiss. Org. Cult.   DOI
18 Variation of alkaloid produtivity among several clones of hairy roots regenerated plants of Atropa belladonna with Agrobacterium rhizogenes 15834 /
[ Aoki,J.;H.Matsumoto;Y.Asako;Y.Matsunaga;K.Shimomura ] / Plant Cell Rep.
19 /
[ George,E.F;P.D.Sherrington ] / Plant Propagation by Tissue Culture
20 Improved media for in vitro culture of Prunus sp. /
[ Quoirin,M.;P.Lepoivre ] / Acta Hort.
21 Enhanced indole alkaloid production in suspension compact callus clusters of Catharanthus roseus: impacts of plant growth regulators and sucrose /
[ Zhao,J.;W.H.Q.Zhu;X.W.He;J.Zhao;W.H.Zhu;Q.Hu;X.W.He ] / Plant Growth Regulation   DOI   ScienceOn
22 Alkaloid production by hairy root cultures in Atropa belladonna /
[ Kamada,H.;N.Okamura;M.Satake;H.Harada;K.Shimomura ] / Plant Cell Rep.   DOI