• Mycobiology
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• v.45 no.4
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• pp.385-391
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• 2017

The ability of Bacillus subtilis, strain ALICA to produce three mycolytic enzymes (chitinase, ${\beta}$-1,3-glucanase, and protease), was carried out by the chemical standard methods. Bacillus subtilis ALICA was screened based on their antifungal activity in dual plate assay and cell-free culture filtrate (25%) against five different phytopathogenic fungi Alternaria alternata, Macrophomina sp., Colletotrichum gloeosporioides, Botrytis cinerea, and Sclerotium rolfesii. The B. subtilis ALICA detected positive for chitinase, ${\beta}$-1,3-glucanase and protease enzymes. Fungal growth inhibition by both strain ALICA and its cell-free culture filtrate ranged from 51.36% to 86.3% and 38.43% to 68.6%, respectively. Moreover, hyphal morphological changes like damage, broken, swelling, distortions abnormal morphology were observed. Genes expression of protease, ${\beta}$-1,3-glucanase, and lipopeptides (subtilosin and subtilisin) were confirmed their presence in the supernatant of strain ALICA. Our findings indicated that strain ALICA provided a broad spectrum of antifungal activities against various phytopathogenic fungi and may be a potential effective alternative to chemical fungicides.

• The Journal of Pediatrics of Korean Medicine
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• v.33 no.3
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• pp.56-68
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• 2019

Objectives The purpose of this study to analyze the effect of Korean medicine therapy of Attention deficit hyperactivity disorders. Methods Electronic research articles were selected by using NDSL, OASIS, KISS, KMBASE, K-portal, Pubmed, Cochrane, and Ebscohost. Results We analyzed fifteen studies about Korean medicine treatment of Attention deficit hyperactivity disorders. There are eleven studies on the acupuncture treatment, and the most commonly used acupoints were GV20 and EX-HN1. There are eight studies on the herb medicine treatment. The most commonly used herbal materials are Root of Rehmanniae Radix et Rhizoma Preparata, Root of Rehmanniae Radix et Rhizoma Preparata, Sclerotium of Poria cocos Wolf, and Rhizome of Acori Gramineri Rhizoma. Syncope and hematomas were common side effects of the acupuncture treatment. Also, the acupuncture needle can be stuck or bent during the treatment. In most studies, Attention deficit hyperactivity disorders was improved when treated with Korean medicine. Conclusions More studies are needed to demonstrate the effectiveness of Korean medicine in Attention deficit hyperactivity disorders. This study can be used for various studies of Attention deficit hyperactivity disorders.

• Korean journal of applied entomology
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• v.15 no.3 s.28
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• pp.153-159
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• 1976

Present paper was attempted to investigate the effects of the light on the sclerotium formation of some isolates of Sclerotinia sclerotiorum(Lib.) do Bary from lettuce, cucumber and rape. The investigations were performed in the both aspects inducing sclerotial primordia from the mycelial colony and developing such primordia. The cultures were grown on potato-dextrose agar and were treated with day-light fluorescent lamps. In the continuous light illumination for 21 days, numbers of sclerotial primordia and matured sclerotia were increased with increasing the light intensity up to about 500 Lux, but on the contrary the dry-weight of matured sclerotia was decreased. In the 800 Lux illumination, induction of the primordia was extremely depressed, and then matured sclerotia were almost not produced. Short-time illumination for 48 hours, although the light intensity was as high as 5000 Lux, increased the numbers of sclerotial primordia and matured sclerotia. Dry-weight of matured sclerotia, however, was slightly increased only at low intensity of 160 Lux. On the other hand, the light shock which u·as alternatively on-of light every one minute for 48 hours increased the number and dry-weight of sclerotia produced, but the former was more effective at 500 Lux shock and the later more effective at 160 Lux shock.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.3
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• pp.475-479
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• 2004

The proteinpolysaccharides (PPS) from Polyporus umbellatus (P. umbellatus) screlotium is composed by 78.2% of saccharide, 16.8% of protein, and 4.0% of ash. PPS from P. umbellatus showed antitumor activities against 180 solid tumor in ICR mice at the concentration of 20-160 mg/kg/day. PPS from P. umbellatus inhibited cell viability to 47.4% and 45.0% in leukemia cell lines, L-1210 and K-562 cells at 50-400 $\mu\textrm{g}$/mL concentration, respectively. But the hall mark of cell apoptosis, DNA fragmentation was not observed at those concentration. 2.5-10.0% of PPS from P. umbellatus inhibited mutagenecity evoked by 2-nitrofluorene and sodium azide in Salmonella typhimurium TA 98 and TA 100. From these results, it is suggested that the PPS of P. umbellatus has antitumor and antimutagenic effect, and its cytotoxic effect may not be ascribed to the apoptosis.

• The Korean Journal of Mycology
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• v.20 no.1
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• pp.44-50
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• 1992

The protoplast formation of Rhizoctonia solani in the fast growing anastomosis groups (AGs) 1 and 4, the intermediate AG-2 and AG-5, and the slow AG-3 yielded the most, moderate and the least in that order, respectively. Sclerotia formation varied with AGs. A high yield of protoplasts from AGs was obtained with a combined lytic enzyme system containing cellulase 'Onozuka' R-10, macerozyme R-10 and ${\beta}-glucuronidase$. When 3g (fresh weight) of 30 hr old mycelia was incubated for 3 hr at $32^{\circ}C$ with the enzyme mixture in 0.6 M mannitol, maximum protoplasts were obtained in the five AGs. A protoplast fusion between sclerotia forming AG-1 inactivated with heat and non-forming AG-5 was induced by polyethylene glycol and ${Ca}^{2+}$. Seven fusants obtained were based on characteristics of colony and sclerotium formation on culture plates. The fusants were confirmed by isozyme patterns of esterase and killing reaction between AG-1 and a fusant F1501.

• The Korean Journal of Mycology
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• v.41 no.1
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• pp.42-46
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• 2013

Burkholderia pyrrocinia CAB08106-4 was parceled out from the Chungnam Agricultural Research and Extension Center, Korea to evaluate the antagonistic activity against garlic white rot caused by Sclerotium cepivorum. The optimum cultural conditions including temperature, pH, enzyme activity, carbon and nitrogen sources were determined. The optimum culture conditions of B. pyrrocinia CAB08106-4 were $286^{\circ}C$, 150 rpm and pH 7. Chitinase only showed activity among several tested enzymes. The highest cell growth was obtained with 1% glucose and 0.1% $(NH_4)_2SO_4$, respectively.

• Applied Biological Chemistry
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• v.37 no.4
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• pp.287-294
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• 1994

New 3-phenyl-1-(2-furyl)propenones, 1 and 3-phenyl-1-(2-furyl)-3-thiophenyl-propanone, 2 derivatives were synthesized, and their fungicidal activities in vitro against Botrytis cineria (BC), Valsa ceratosperma (VC), Scelerotium cepivorum (SC) and Phytophthora capsici (PC) were investigated using a generalized structure-activity relationship (SAR). The activity of 1 was superior to those of 2, and nonsubstituent, 1a and chloro group substituent, 1d of E (Syn) conformer were the most effective $(EC_{50}=10{\sim}12\;ppm)$ compound to BC. Antifungal activities were able to predict to depend essentially on the ${\beta}$ carbon and their positive charge from the results that the good correlation $(r^2=0.90)$ was observed between hydrolysis rate constant (logk) of 1 and the electronic parameter $({\sigma})$ of X-substituent on the ${\beta}-phenyl$ ring.

• The Korean Journal of Pesticide Science
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• v.5 no.1
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• pp.1-11
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• 2001

Climate in the year of 2000 was characterized as a long severe drought in tile spring, unusually high and low temperature in summer, two times of typoons, and floods by heavy rains in fall. Rice leaf and panicle blast and bacterial grain rot occurred severely comparing with 1999 and Bipolaris leaf spot spread over tile country. Phytophthora blight and anthracnose in red-pepper became epidemic especially in the late season causing severe yield losses. Tomato fusaruim wilt, CGMMV, powdery mildew, and sudden wilt syndrom of cucurbits and strawberry powdery mildew were also severe in 2000. In garlic, sclerotium rot occurred severely mainly due to the frequent rainfalls in planting time and much snowfalls in 1999's winter. Spring potato had severe infection of viruses due to a long spring drought, and fall potato had high incidence of bacterial soft rot and bacterial wilt due to fall floods by heavy rains. In sweet potato fusarium wilt was the most severe as in other year. Disease incidence of apple and pear trees was rotatively mild compared with previous years. In wheat and barley, Gibberella petch rarely occurred because of spring drought.

• Weed & Turfgrass Science
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• v.7 no.3
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• pp.269-274
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• 2018

In the middle of May 2018, typical red thread disease symptoms were observed on Kentucky bluegrass (Poa pratensis L.) on a golf course, which locates at Yangsan, Gyeongnam province in Korea. Irregular-shaped patched symptoms were observed in fairway of golf course. The foliar symptom was dried out and faded to straw color and tip of the grass leaves were tangled like thread. Early morning, infected and tangled leaves were covered with the pinkish gelatinous antler-like structure (sclerotinia) as a typical red thread disease symptom. Causal fungal pathogens were isolated from the symptom in Kentucky bluegrass. The fungal culture characteristic on potato dextrose agar color of colony was pale pink and conjugated hyphae, sclerotium of irregular shape was pale pink and 3~5mm diameter in size. The pathogen was identified as Laetisaria fuciformis based on morphological and culture characteristics as well as molecular characteristics. Pathogenicity test was verified on the Kentucky bluegrass by Koch's postulates. This is the first report of red thread disease occurrence in Kentucky bluegrass by L. fuciformis in Korea.

• Research in Plant Disease
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• v.12 no.3
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• pp.267-271
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• 2006

Botrytis cinerea Pers. was found to be highly virulent to the grapevine plant, especially in greenhouse condition. Pseudomonas species play key roles for the biocontrol of many plant diseases especially in soil. Of the 83 isolates of Pseudomonas spp., a bacterial strain P84, isolated from tomato rhizosphere, was shown to suppress a wide range of phytopathogenic fungi in vitro. The isolate was identified as Pseudomonas putida on the basis of its bacteriological and genetic characteristics. The P. putida P84 strain carry the phlD gene for 2,4-diacetylphloroglucinol biosynthesis and may produce the antibiotics as an antagonistic mechanism involved in biocontrol. The antagonistic activity of the bacterium has a promising implication for its use as a biocontrol agent to control grapevine gray mold.