Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Selection of Antagonistic Bacteria for Biocontrol of Botrytis cinerea Causing Gray Mold on Vitis spp

포도 잿빛곰팡이병의 생물적 방제를 위한 길항세균 선발

  • Seo, Sang-Tae (Horticultural Environment Division, National Horticultural Research Institute, Rural Development Administration) ;
  • Park, Jong-Han (Horticultural Environment Division, National Horticultural Research Institute, Rural Development Administration) ;
  • Han, Kyoung-Suk (Horticultural Environment Division, National Horticultural Research Institute, Rural Development Administration) ;
  • Cheong, Seung-Ryong (Horticultural Environment Division, National Horticultural Research Institute, Rural Development Administration)
  • Published : 2006.12.01
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Abstract

Botrytis cinerea Pers. was found to be highly virulent to the grapevine plant, especially in greenhouse condition. Pseudomonas species play key roles for the biocontrol of many plant diseases especially in soil. Of the 83 isolates of Pseudomonas spp., a bacterial strain P84, isolated from tomato rhizosphere, was shown to suppress a wide range of phytopathogenic fungi in vitro. The isolate was identified as Pseudomonas putida on the basis of its bacteriological and genetic characteristics. The P. putida P84 strain carry the phlD gene for 2,4-diacetylphloroglucinol biosynthesis and may produce the antibiotics as an antagonistic mechanism involved in biocontrol. The antagonistic activity of the bacterium has a promising implication for its use as a biocontrol agent to control grapevine gray mold.

Botrytis cinerea에 의한 포도 잿빛곰팡이병은 특히 하우스 재배시 큰 피해를 주는 병원 진균이다. Pseudomonas속 세균들은 토양 미생물중 가장 잘 연구되어 있고, 토양 내에서 중요한 역할을 담당하고 있다. 근권토양에서 분리한 형광성 Pseudomonas속 세균 83균주 중 P84균주는 실내 항균력 실험결과 다양한 식물병원진균(Phytophthora capsici, Sclerotium spp., Botryosphaeria dothidea, Fusarium spp.)에 대해 항균효과를 나타내었다. 생리적 실험과 유전적 실험결과 P84균주는 P. putida로 동정되었다. 이 세균의 항균력은 항생물질(2,4-diacetylphloroglucinol)의 생산과 관련되어 있는 것으로 사료되며, 이 세균이 포도 잿빛곰팡이병의 생물적 방제에 이용될 수 있는 가능성이 시사되었다.

Keywords

References

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