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검색결과 31,197건 처리시간 0.061초

Analysis of Genetic Variation in Botrytis cinerea Isolates Using Random Amplified Polymorphic DNA Markers

  • Choi, In-Sil;Kim, Dae-Hyuk;Lee, Chang-Won;Kim, Jae-Won;Chung, Young-Ryun
    • Journal of Microbiology and Biotechnology
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    • 제8권5호
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    • pp.490-496
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    • 1998
  • Random amplified polymorphic DNA (RAPD) markers were used to survey genetic variability among 34 Botrytis cinerea isolates from nine different host plants in Korea. For RAPD analysis, 115 arbitrary decamer primers were initially screened for polymorphic major DNA bands with 11 representative B. cinerea isolates. Eleven primers that initially detected polymorphisms were tested a second time with additional 23 isolates of B. cinerea as well as one isolate of Botrytis squamosa as an outgroup. The RAPD analyses revealed that all isolates except one showed different molecular phenotypes. Dendrograms obtained from dissimilarity matrices using the unweighted paired group method of arithmetic means (UPGMA) showed the 36.4% to 90.0% similarity among all B. cinerea isolates. The B. squamosa isolate showed the least similarity to all B. cinerea isolates. The cluster analyses indicated no correlation among all the characteristics examined including molecular phenotypes, host and geographic origins, year of isolation, or pathogenicity. The RAPD data suggest that a high level of genetic variation exists among Korean populations of B. cinerea and it seems to be caused by heterokaryosis among preexisting molecular phenotypes.

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AllEC: An Implementation of Application for EC Numbers Prediction based on AEC Algorithm

  • Park, Juyeon;Park, Mingyu;Han, Sora;Kim, Jeongdong;Oh, Taejin;Lee, Hyun
    • International Journal of Advanced Culture Technology
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    • 제10권2호
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    • pp.201-212
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    • 2022
  • With the development of sequencing technology, there is a need for technology to predict the function of the protein sequence. Enzyme Commission (EC) numbers are becoming markers that distinguish the function of the sequence. In particular, many researchers are researching various methods of predicting the EC numbers of protein sequences based on deep learning. However, as studies using various methods exist, a problem arises, in which the exact prediction result of the sequence is unknown. To solve this problem, this paper proposes an All Enzyme Commission (AEC) algorithm. The proposed AEC is an algorithm that executes various prediction methods and integrates the results when predicting sequences. This algorithm uses duplicates to give more weights when duplicate values are obtained from multiple methods. The largest value, among the final prediction result values for each method to which the weight is applied, is the final prediction result. Moreover, for the convenience of researchers, the proposed algorithm is provided through the AllEC web services. They can use the algorithms regardless of the operating systems, installation, or operating environment.

Effects of Defaunation on Fermentation Characteristics and Methane Production by Rumen Microbes In vitro When Incubated with Starchy Feed Sources

  • Qin, W.Z.;Li, C.Y.;Kim, J.K.;Ju, J.G.;Song, Man-K.
    • Asian-Australasian Journal of Animal Sciences
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    • 제25권10호
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    • pp.1381-1388
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    • 2012
  • An in vitro experiment was conducted to examine the effects of defaunation (removal of protozoa) on ruminal fermentation characteristics, $CH_4$ production and degradation by rumen microbes when incubated with cereal grains (corn, wheat and rye). Sodium lauryl sulfate as a defaunation reagent was added into the culture solution at a concentration of 0.000375 g/ml, and incubated anaerobically for up to 12 h at $39^{\circ}C$. Following defaunation, live protozoa in the culture solution were rarely observed by microscopic examination. A difference in pH was found among grains regardless of defaunation at all incubation times (p<0.01 to 0.001). Defaunation significantly decreased pH at 12 h (p<0.05) when rumen fluid was incubated with grains. Ammonia-N concentration was increased by defaunation for all grains at 6 h (p<0.05) and 12 h (p<0.05) incubation times. Total VFA concentration was increased by defaunation at 6 h (p<0.05) and 12 h (p<0.01) for all grains. Meanwhile, defaunation decreased acetate and butyrate proportions at 6 h (p<0.05, p<0.01) and 12 h (p<0.01, p<0.001), but increased the propionate proportion at 3 h, 6 h and 12 h incubation (p<0.01 to 0.001) for all grains. Defaunation increased in vitro effective degradability of DM (p<0.05). Production of total gas and $CO_2$ was decreased by defaunation for all grains at 1 h (p<0.05, p<0.05) and then increased at 6 h (p<0.05, p<0.05) and 12 h (p<0.05, p<0.05). $CH_4$ production was higher from faunation than from defaunation at all incubation times (p<0.05).

반도체 광 증폭기를 이용한 전광 데이터 추출 (All-optical Data Extraction Based on Optical Logic Gates)

  • 이지석;정미;이혁재;이택진;전영민;이석;우덕하;이주한;김재헌
    • 한국광학회지
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    • 제23권4호
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    • pp.143-146
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    • 2012
  • 본 논문에서는 반도체 광 증폭기(Semiconductor Optical Amplifier)의 교차 진폭 변조 현상을 이용하여 광 컴퓨팅이나 광통신에서 데이터의 패킷 교환, 데이터 리셋 혹은 추가나 제거에 필수적인 전광 데이터 추출을 시뮬레이션하고 실험적으로 구현하였다. 전광 데이터 추출의 실험적 시뮬레이션에는 상용 프로그램인 VPI Tool을 사용하였으며 기본적으로 두 개의 반도체 광 증폭기(SOA)를 사용하여 AND 논리를 구성한 후 이를 이용하여 데이터를 추출하였다. 또한 높은 집적성과 효율로 고속으로 연산할 수 있는 가능성을 확인하였다.

ADVANTAGES OF THE AKARI FIR ALL-SKY MAPS

  • Doi, Yasuo;Takita, Satoshi;Ootsubo, Takafumi;Arimatsu, Ko;Tanaka, Masahiro;Morishima, Takahiro;Kawada, Mitsunobu;Matsuura, Shuji;Kitamura, Yoshimi;Hattori, Makoto;Nakagawa, Takao;White, Glenn;Ikeda, Norio
    • 천문학논총
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    • 제32권1호
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    • pp.11-15
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    • 2017
  • We present the AKARI far-infrared (FIR) all-sky maps and describe its characteristics, calibration accuracy and scientific capabilities. The AKARI FIR survey has covered 97% of the whole sky in four photometric bands, which cover continuously 50-180 micron with band central wavelengths of 65, 90, 140, and 160 microns. The data have been publicly released in 2014 (Doi et al., 2015) with improved data quality that have been achieved since the last internal data release (Doi et al., 2012). The accuracy of the absolute intensity is ${\leq}10%$ for the brighter regions. Quantitative analysis of the relative intensity accuracy and its dependence upon spatial scan numbers has been carried out. The data for the first time reveal the whole sky distribution of interstellar matter with arcminute-scale spatial resolutions at the peak of dust continuum emission, enabling us to investigate large-scale distribution of interstellar medium in great detail. The filamentary structure covering the whole sky is well traced by the all-sky maps. We describe advantages of the AKARI FIR all-sky maps for the study of interstellar matter comparing to other observational data.

Assessment of the Non All-in All-out System and the All-in All-out System for Sows and Their Suckling Piglets

  • Jeon, J.H.;Yeon, S.C.;Kim, S.;Kim, D.H.;Chang, H.H.
    • Asian-Australasian Journal of Animal Sciences
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    • 제19권6호
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    • pp.909-914
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    • 2006
  • The goal of this study was to investigate the behavior of sows and their piglets reared using the non-all-in all-out system and the all-in all-out system during a 3-week lactation period. The 24 Landrace${\times}$Yorkshire sows (range of parity: 2 to 4) and their litters (range of litter size: 8 to 13 piglets) for each treatment (8 sows/replication) were used in this experiment. The sows were housed in farrowing crates ($0.6{\times}2.1m$) located in pens ($2.4{\times}1.8m$) with totally perforated metal flooring. The crates were kept in an experimental room that was thermostatically controlled to approximately $23^{\circ}C$. A 250-W infrared heat lamp was turned on above the creep area during lactation. The sows and piglets were conventionally managed. The animals were recorded during a 24-h period at 1, 4, 7, 14 and 21 days postpartum. The videotapes were scanned every minute to obtain an instantaneous behavioral sample. In the behaviors of sows, lateral recumbency decreased and ventral recumbency increased from 1 day postpartum to 21 days postpartum. In comparison to the AIAOS, sows in the NAIAOS spent much less time on lateral recumbency (p<0.01), whereas they spent much more time on ventral recumbency and sitting at 4 days postpartum (p<0.05 and p<0.05). In the behaviors of piglets, lying increased and unsuccessful suckling decreased until 21 days postpartum. Compared to the AIAOS at 4 days postpartum, piglets in the NAIAOS spent less time lying (p<0.01), whereas they spent much more time walking and unsuccessful suckling (p<0.01 and p<0.05). These results suggest that the all-in all-out system may be preferable to the non-all-in all-out system for promoting welfare.

재난대응 황금시간 목표제의 효과성에 관한 연구 서울특별시 마포소방서 현장대응 소방공무원의 인식을 중심으로 - (A Study on the Effectiveness of Golden Time Targeting in the Field of Disaster Response : Focusing on Mapo Fire Fighters' Realization)

  • 이은와;윤명오;박찬석
    • 대한안전경영과학회지
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    • 제19권4호
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    • pp.1-9
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    • 2017
  • In recent years after the Sewol Sank, the surroundings of Fire have rapidly changed and citizens need for Fire have been demanding very high. Under these situations, in order for satisfaction to all these demands, Seoul Metropolitan Fire & Disaster Headquarters(SMFDH) have made improvements of Golden Time in the field of disaster as the leader of Fire Department in Korea. SMFDH has been practicing many kinds of plans, or projects to achieve Gold Time of 55 types of disaster. However, There have been questioning about all these projects if they are working for the achievement. The purpose of this study is to scrutinize current projects for Golden Time Targeting focused on Mapo fire fighters as first responders and to cope with how to make them better for Seoul Citizens' safety by using SPSS(Statistical Package for the Social Science) Statistics 24. The result of this study presents that how we can find to be all the project for Golden Time Targeting running well. And This study will be positively applied to Seoul fire policies or helped for setting them up better.

Height Growth Models for Pinus thunbergii in Jeju Island

  • Park, Gildong;Lee, Daesung;Seo, Yeongwan;Choi, Jungkee
    • Journal of Forest and Environmental Science
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    • 제31권4호
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    • pp.255-260
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    • 2015
  • Height growth models for Pinus thunbergii in Jeju Island were developed in this study using four widely used nonlinear growth models; Exponential, Modified Logistic, Chapman-Richards, and Weibull. All functions were found to be significant at the 1% level. Chapman-Richards model for height-DBH allometry and Weibull model for height-age allometry was chosen as the best model on the all validation. All the model curves showed the similar pattern. Additionally, there was no abnormal pattern when the previous studies were compared. Therefore, these models are highly expected to be used to estimate the tree height using DBH or age for Pinus thunbergii especially in Jeju Island.

경호전공 대학생의 전공만족, 진로정체감 및 진로결정 자기효능감의 구조적 관계 (Structural Relations of Security Science Majors' Major Satisfaction, Career Identity and Career Decision-making Self Efficacy)

  • 염대관;김창호;김우진
    • 시큐리티연구
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    • 제43호
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    • pp.121-145
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    • 2015
  • 본 연구는 경호전공 대학생 중심으로 전공만족, 진로정체감 및 진로결정 자기효능감의 구조적 관계를 규명하는데 그 목적이 있다. 또한 각 변인들간의 직접효과, 간접효과와 총효과를 확인하여 합리적인 진로탐색과 진로프로그램 개발의 기초를 제공해주고자 한다. 이를 위해 경호전공 대학생 239명의 설문자료를 수집하였으며 구조방정식 모형분석을 적용하였다. 연구결과를 요약하면 다음과 같다. 첫째, 교과만족은 진로결정, 진로확신, 진로흥미에 유의한 영향을 미치는 것으로 나타났다. 둘째, 관계만족과 일반적 만족은 진로결정과 진로확신에 유의한 영향을 미치는 것으로 나타났다. 셋째, 사회적 인식은 진로흥미에 유의한 영향을 미치는 것으로 나타났다. 넷째, 진로결정, 진로확신은 문제해결, 정보수집, 계획설정에 유의한 영향을 미치는 것으로 나타났으며 진로흥미는 문제해결과 정보수집에 유의한 영향을 미치는 것으로 나타났다. 다섯째, 교과만족은 진로결정을 통하여 문제해결과 정보수집에 매개효과를 가지는 것으로 나타났다. 여섯째, 교과만족은 진로확신을 통하여 문제해결과 정보수집에 매개효과를 가지는 것으로 나타났다. 일곱째, 관계만족은 진로흥미를 통하여 문제해결, 정보수집과 계획설정에 매개효과를 가지는 것으로 나타났다. 여덟째, 일반적 만족은 진로결정을 통하여 문제해결과 정보수집에 매개효과를 가지는 것으로 나타났다. 이상의 연구결과를 중심으로 경호학에서의 시사점과 추후연구를 위한 제언들을 논의하였다.

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