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Indoor Tank Culture of the Abalone Haliotis discus hannai II. Effects of diets on the growth of young abalone (참전복 Haliotis discus hannai의 육상수조사육에 관한 연구 II. 먹이별 사육실험)

  • JEONG Seong-Chae;JEE Young-Ju;SON Pal-Won
    • Journal of Aquaculture
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    • v.7 no.2
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    • pp.77-87
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    • 1994
  • The study was conducted to develop the suitable abalone's diets for the indoor tank culture. The young abalone Haliotis discus hannai Ino sized ca. 20, 30 and 40 nun in shell length were fed three different diets, dried sea mustard Undaria pinnatifida HARVEY (control), artificial diet and terrestrial plants e. g. Chinese cabbage and Perilla leaf for 2 years. The growth rate of abalones fed an artificial diet was slightly higher than that of abalones from the control. However, the growth rate of abalones from terrestrial plants was significantly lower than that of abalones fed a control or an artificial diet (P< 0.05). The daily feeding rate of young abalones from terrestrial plants was slightly higher than that of abalones from the control. While, that of abalones from an artificial diet was approximately $30\%$ of that of abalones from a control or a terrestrial plants diet. The feed efficiency of abalone from artificail diet was higher than that of abalones from a control or a terrestrial plants diet, and the smaller size of young abalone showed the higher the feed efficiency. The EFA index values ${{\sum}n9/(20:4n6+20:5n3+22:6n3}$ of the dried sea mustard and the artificial diet were 1.26 and 3.64, respectively. These values were favorable, but the value of terrestrial plant, Perilla leaf was 127.00 indicating poor EFA index for normal growth of abalone.

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Uncoupling Protein 3 in the Rainbow Trout, Oncorhynchus mykiss Sequence, Splicing Variants, and Association with the AvaIII SINE element

  • Kim, Soon-Hag;Choi, Cheol-Young;Hwang, Joo-Yeon;Kim, Young-Youl;Park, Chan;Oh, Berm-Seok;Kimm, Ku-Chan;Scott A. Gahr;Sohn, Young-Chang
    • Journal of Aquaculture
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    • v.17 no.1
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    • pp.1-7
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    • 2004
  • A rainbow trout uncoupling protein 3 (UCP3) cDNA clone, encoding a 310 amino acid protein, was cloned and sequenced from a liver cDNA library. Two different splice variants designated UCP3-vl and UCP3-v2, were identified through liver cDNA library screening using rainbow trout UCP3 cDNA clone as a probe. UCP3-vl has 3 insertions in the UCP3 cDNA: the first insertion (133 bp), the second (141 bp), and the third (370 bp) were located 126 bp, 334 bp and 532 bp downstream from the start codon, respectively. UCP3-v2 contained a single insertion, identical in sequence and location to the second insertion of UCP3-vl. UCP3, a mitochondrial protein, functions to modulate the efficiency of oxidative phosphorylation. UCP3 has been detected from heart, testis, spinal cord, eye, retina, colon, muscle, brown adipose tissue and white adipose tissue in mammalian animals. Human and rodent UCP3s are highly expressed in skeletal muscle and brown adipose tissue, while they show weak expression of UCP3 in heart and white adipose tissue. In contrast to mammalian studies, RT-PCR and Southern blot analysis of the rainbow trout demonstrated that UCP3 is strongly expressed in liver and heart. UCP3, UCP3-vl, and UCP3-v2 all contain an Ava III short interspersed element (SINE), located in the 3'untraslated region (UTR). PCR using primers from the Ava III SINE and the UCP3 3'UTR region indicates that the UCP3 cDNA is structurally conserved among salmonids and that these primers may be useful for salmonid species genotyping.

A Case Analysis of Home Health Care for Cesarean Postpartum Women and Their Newborns (제왕절개분만 산욕부와 신생아의 가정간호 사례분석)

  • 김혜숙;최연순;전은미
    • Journal of Korean Academy of Nursing
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    • v.24 no.4
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    • pp.696-705
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    • 1994
  • This study was done to provide a basis for home health care management for women following Cesarean delivery. Furthermore it was initiated as an possible application of home health care in the future. In this study, client selection criteria was developed by the researcher and assessment tools for home health care, recording system and problem oriented recording system were revised from Jun's(1993) methods. The selection criteria tool for home health care for women who had a Cesarean delivery was structured and consisted of five areas : physical status, functional status, psychological-emetional status, educational needs status and environmental status. The structured assessment tool consisted of general items, obstetric history, past medical history, methods of feeding, medications taken before ad-mission, laboratory results, discharge instructions, discharge medications, family tree, economic status, environmental status, a map, health assessment of women and their newborns. The visit note consisted of the date : nursing problems : nursing process including initial assessment : nursing goals : visit plan : health status of the postpartum women and their newborn : nursing diagnoses : nursing implementation evaluation : summary : next visit plan and revision. The problem oriented recording system consisted of the date, problem numbers, nursing diagnoses, problem appearance date, problem resolution date. The results of the research are as follows : The seven cases having had a Cesarean delivery were discharged on an average on the 5th day after the Cesarean birth. The total number of home visits was 13. According to Cordon's functional health patterns the total possible nursing diagnoses was 34 diagnoses for the methers and their newborns. Among the 34 diagnoses, there were 13 diagnoses in the health perception /management pattern, 7 in the psychosocial health perception / management pattern, 8 in the psychosocial self-perception, 2 in the nutrition / metabolism pattern of physical function, 2 in the knowledge deficit of newborn management, anxiety related to newborn management, knowledge deficit related to disease process of new-born, anxiety related to disease process of newborn anxiety related to prognosis of baby's condition, knowledge deficit related to newborn jaundice each appeared once. The changes in the number of nursing diagnoses was related to not the number of visits but to the number of nursing diagnoses decreasing. The con-tent of the home health care was categorized ac-cording to assessment, direct care, counseling, education, family care. The recommendation based on the results of this research are Home health care nurses for Cesarean postpartum women and their neonates requires comprehensive knowledge of pregnancy, delivery, and the postpartum period and of the neonate so that they can provide appropriate care and holistic views. Most of cases terminated after the second visit, this outcome may be related to the subjects being discharged on the 5th day after delivery. Therefore, study done with earlier discharge after delivery may have different outcome. It is very hard to assess psychological aspects that need follow-up and to develop communication channels.

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A study of Expression of TGF-β1, c-Myc, Erb-B2 and Thymosin-β4 Gene in Alcoholic Liver Damage Tissue. (알코올성 간 손상 조직에서 TGF-β1와 c-Myc, Erb-B2, Thymosin-β4 유전자 발현 융합 연구)

  • Kim, Jean-Soo;Choi, Sang-Ki
    • Journal of the Korea Convergence Society
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    • v.9 no.5
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    • pp.91-97
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    • 2018
  • This study has been conducted to see the expression of $TGF-{\beta}_1$, c-Myc, Erb-B2 and $Thymosin-{\beta}_4$ genes in ethanol - damaged liver tissues. Experimental groups were divided into 2 groups, one where damaged liver was caused by 25% ethanol and normal group administered with purified water. Results of test showed the expression of $TGF-{\beta}_1$, c-Myc, and $Thymosin-{\beta}_4$ genes was higher in the experimental group treated with 25% ethanol than in the normal group. Erb-B2 gene was not expressed clearly. Thus, it is considered that we can expect to utilize $TGF-{\beta}_1$, c-Myc 및 $Thymosin-{\beta}_4$ as auxiliary data and find clinical meanings of diagnosis on hepatic diseases, In addition to serologic and histological examination by convergence examining the gene expression status by molecular diagnostic techniques in liver-related disease prevention and diagnosis through results of this study.

Egg Development and Early Life History of the Korean Southern Spine Loach, Iksookimia hugowolfeldi (Pisces: Cobitidae) (한국고유종 남방종개 Iksookimia hugowolfeldi (미꾸리과)의 난발생 및 초기생활사)

  • Ko, Myeong-Hun;Bang, In-Chul
    • Korean Journal of Ichthyology
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    • v.28 no.3
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    • pp.156-163
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    • 2016
  • Egg development and early life history of Korean southern spine loach, Iksookimia hugowolfeldi, were observed in the present study. Eggs were obtained after injecting females with 0.5 mL/kg of Ovarprim. Eggs were artificially fertilized using the dry method in the laboratory. Number of spawned eggs were $1,933{\pm}530per$ individual. Mature eggs were slightly adhesive with light yellowish coloring, and they measured $1.35{\pm}0.03mm$ in diameter. Hatching of the embryo occurred 56 h (50%) after fertilization at water temperature $25^{\circ}C$, and newly hatched larvae were averaged of $5.6{\pm}0.18mm$ in total length. At 5 days after hatching, larvae averaged $7.8{\pm}0.31mm$ in total length and their yolk sacs had been completely absorbed. Beginning at 15 days after hatching, the fish entered the juvenile stage and reached $13.2{\pm}0.87mm$ in total length. At 100 days after hatching, the band patterns and external form of juvenile were similar to those of adults, and they averaged $49.2{\pm}4.29mm$ in total length.

Effects of Civil Blasting on Noise, Vibration and Total Suspended Particles (토목 발파가 소음, 진동, 부유 분진에 미치는 영향)

  • Jeong, Jin Do;Jeong, Yeong Guk
    • Journal of Environmental Science International
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    • v.13 no.1
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    • pp.99-107
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    • 2004
  • This research is to determine the level of environmental pollution at a blasting construction area which is the origin of noise, vibration, and suspended particle, and to compare the results with other domestic and international standard data. This experiment is also to find out the effects resulting from blasting construction and to propose a plan that can decrease environmental pollution. The blasting construction area is a factory site which is about one and half million square meter and sewage disposal plant is about ninety thousand square meter. Both were selected as the areas for the tests to be conducted in determination test. The test to determine the level of noise, vibration, and total suspended particle was conducted thirty times around the blasting construction area by comparing measurement results and numerical analysis. However, as the test was not conducted in the laboratory but in the actual blasting construction area, it was not possible to do the test with the same exact conditions each true. Therefore, the test was not ideal as conditions could change from test to test. For the most part, the level of noise was below the standard level of 70dB. Every vibration test was under the standard limitation. For example, a house, 200m away was tested for noise and vibration and the level was found to be under the 0.2 cm/sec which is the standard for specialty designed cultural sites., i.e very low level. Also a buried oil pipeline that was 30m away also marked under 2.0cm/sec which is the norm for an industrial area. However, if there were an oil pipeline under the house, the amount of charging gunpowder per hole should be decreased compared to the amount used in the test. The test result for suspended particles under the standard limitation which is 24hour average 300$\mu\textrm{g}$/㎥ at a distance from blasting wavelength, but at detonator, total suspended particle from the blast origin exceeded the standard limitation. If explosion occurs when it detonates in the hole, most of the energy would be absorbed in the crushing of rocks, but some remaining energy would make noises and vibration inevitable. So the important thing is how to minimize the environmental pollution from the blasting. There should be regulations in order that the standard limitation is not exceeded, and to decrease the environmental pollution from the blasting.

Applicability of a Photosynthetic Ciliate, Mesodinium rubrum MR-MAL01 -Usefulness as a Live Prey Species for the Marine Aquaculture of Bivalves- (광합성 섬모류 Mesodinium rubrum MR-MAL01의 응용성(1) 이매패류 종묘생산을 위한 먹이생물 유용성)

  • 김형섭;명금옥;조수근;이원호
    • Journal of Aquaculture
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    • v.17 no.2
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    • pp.115-121
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    • 2004
  • In a series of feeding experiments using the newly established the first laboratory strain of a temperate photosynthetic ciliate, Mesodinium rubrum MR-MAL01, direct evidence was obtained for ingestion of the cultured M. rubrum cells by the bivalves like the Korean scallop, Chlamys farreri and Manila clam. Ruditapes phil-ippinarum. Each experimental Korean scallop and Manila clam removed up to 9,590 and 23,200 cells min-1, respectively. Small particles almost identical to the ruptured cells of MR-MAL01 culture were observed in the gut of the experimental bivalves, although no intact M. rubrum cell was found. In a 28 days rearing experiment, Isoshrysis galbana (KMCC H-2), a microalga, supported better growth of small Manila clam spat (0.46 mm in shell length) than MR-MAL01 strain. For the large Manila clam spat (0.84 mm in shell length), however. MR-MAL01 strain was a better prey item. Mass culture methods for this photosynthetic marine ciliate may be developed for use as live feed in aquaculture of the spat and broodstock of bivalve and small-mouthed fish larvae.

Development of an Experimental Model for Vitamin C Requirement Study in Korean Rockfish, Sebastes schlegeli (조피볼락(Sebastes schlegeli)의 사료내 비타민 C 요구량 설정을 위한 기초연구)

  • Bai Sungchul C.;Lee Kyeong-Jun;Jang Hye-Kyung
    • Journal of Aquaculture
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    • v.9 no.2
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    • pp.169-178
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    • 1996
  • This experiment was conducted to develop an experimental model and a semipurified diet for vitamin C requirement study in juvenile Korean rockfish, Sebastes schlegeli. Prior to the start of the feeding trial, fish were fed the control diet for four weeks to deplete their body reserves of vitamin C, Then fish were divided into six groups and given one of the laboratory developed semipurified diets supplementing either 0 (control), 25, 50, 75, 150, o. 1500 mg L-ascorhic acid (AA)/kg diet for eight weeks. Fish fed control diet had lower hematocrit than did fish from groups fed the other diets (P<0.05). Also these fish had significantly lower percent weight gain (WG), feed conversion ratio (FCR) and muscle AA than did fish fed diets containing 150 and 1500 mg AA/kg diet (P<0.05). Diet analysis of vitamin C showed that the control diet had 39,7 mg AA/kg diet. Therefore, these fingings suggest that the experimental model and the semipurified diet can be useful for vitamin C requirement study, and the dietary vitamin C requirement is greater than 40 mg AA/kg diet, but 150 mg AA/kg diet is adequate for the maximum growth in juvenile Korean rockfish.

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Tylosin Production by Mutant Resistant to Oleic Acid (Oleic Acid 내성균주로부터 Tylosin 생산)

  • Choi, Du-Bok;Choi, On-You;Moon, Ok-Ran;Yoon, Mi-Ran;Ji, Sung-Nam;Shin, Dae-Yewn
    • Journal of Environmental Health Sciences
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    • v.31 no.5 s.86
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    • pp.360-364
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    • 2005
  • When rapeseed oil as the carbon source was used for tylosin production from Streptomyces fradiae TP-1239 was very sensitive to oleic acid. Cell growth was restrained by adding 0.8 g/l of oleic acid to the culture broth. Mutant strain TM-224-1 resistant to 1.2 g/l of oleic acid was obtained by screening in solid and liquid media containing oleic acid. The uptake rate of oleic acid by TM-224-1 was approximately 3.8 fold higher than the parent strain. For comparing the TM-224-1 and the parent strain, batch cultures were carried out in a jar fermentor. Cell growth of TM-224-1 strain was higher than the parent strain after two days of culturing. However, after four days of culturing, it was similar to that of the parent strain. The amount of rapeseed oil consumed by TM-224-1 and the parent strain were 60.5 and 78.2 g/l, respectively. The production and yield of tylosin was aproximately 2.0 and 3.2 fold higher than the parent strain, respectively. From these results, it was concluded that this mutant, which was resistant to oleic acid, has improved tylosin production.

Development of technology in estimating of high-risk driver's behavior (고위험군 운전자의 운행행태 판단기술 개발)

  • Jin, Ju-Hyun;Yoo, Bong-Seok;Lee, Wook-Soo;Kim, Gyu-Ho
    • The Journal of the Korea institute of electronic communication sciences
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    • v.11 no.5
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    • pp.531-538
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    • 2016
  • Driving behaviors such as speeding and illegal u-turn which violate traffic rules are main causes of car accidents, and they can lead to serious accidents. Bus drivers are less aware of dangers of illegal u-turn, and infrastructures such as traffic enforcement equipment and watchmen are deficient. This research aims to develop technology for estimating driving behaviors based on map-matching in order to prevent illegal u-turns. For this research, 23,782 of u-turn permit data and 146,000 of speed limit data are collected nationwide, and an estimation algorithm is built with these data. Then, an application based on android is developed, and finally, tests are conducted to assess the accuracy in data computations and GPS data map-matching, and to extrapolate driving behavior. As a result of the tests, the accuracy results in the map-matching is 86% and the assessment of driving behavior is 83%, while the display of the data output yielded 100% accuracy. Additional research should focus on improvement in accuracy through the development of a robust monitoring system, and study of service scenarios for technology application.